LebrA.19448.a

Mitogen-activated protein kinase (EC 2.7.11.24)

CENTER ID: LebrA.19448.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.19448.a.HZ2.GE43706 kinase domain 1 359
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.35.0840
UniProt: A4HNT2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNEIESHIL KKYEIQTQLG QGAYGIVWRA VERKYNRIVA LKKIYDAFQN STDAQRTFRE IMFLHRLHHP NIIKLLHVHR AYNDRDIYLV FEYMETDLHV VIRANILEEI HKQFIMYQLL KTMKYLHSAE ILHRDMKPSN LLINSDCTMK VADFGLARSI LSLESEQASR PVLTDYIATR WYRPPEILLG STRYTKGVDM WSVGCILGEL MLGKPIFPGR STTNQLELIC SVTGMPSSAD VAATNSQFAH AMLRDIHLSH RRTFAELLPN ASADALDLIQ RLMCFNPNRR ISAKEALGHP YVAAFHRPDE EPVAPDPITI SLPDNERLPL DKYRDAIYEQ IAALRRCSAS GEQRQRMERQ ASSGTPSRKT STSSGAAAAA AATSGSRAGT SSSTITRSNA SSSAAVRVVP QRSTVKSTST SGVNDSVPSK TYARPAFRQA APSTTAIESR PVARETVVRK
NT Sequence
atgtcgaacg agattgagtc gcatattcta aagaaatatg agattcaaac gcagctagga cagggtgcat acggcatcgt ttggcgcgcc gtggagcgca aatacaaccg catcgttgcg ctcaagaaga tctacgacgc ttttcaaaac tcgacagatg cgcagcgcac cttccgtgag attatgttct tgcaccggct gcaccacccc aacatcatca agcttctgca cgtccatcgc gcctacaatg atcgcgatat ctaccttgtc ttcgaataca tggagacgga cctgcacgtg gtgattcggg caaacatctt ggaagaaatt cacaagcagt tcatcatgta ccagctgctc aagacgatga agtacctgca ctctgccgag attcttcacc gtgacatgaa gccaagcaac ctgcttatca acagcgactg cacgatgaag gtggctgact tcggtctcgc gcgctccatc ctttcacttg agagtgagca ggcgtcgcgt ccggtgctaa cagactacat tgcgacgcgg tggtaccgtc cgccggagat tctgcttgga tcgacacgat acactaaggg cgtagatatg tggtcggtgg gctgcattct tggagagctg atgcttggca agcccatctt tcccggccgc tccaccacaa accagttaga gcttatctgc agcgtgacag gcatgccttc ctcggcggat gtggcggcga ccaactcgca gtttgcccac gccatgcttc gcgacatcca cctctcgcac cgacgcacat tcgccgaact tctgccaaac gcttctgcgg atgcgttgga ccttatccag cgcctgatgt gcttcaaccc aaaccggcgc atcagtgcga aagaggctct gggacacccg tacgtggctg cctttcaccg tcctgacgag gagccggtgg cgcccgatcc catcaccatc tctctccctg acaacgagcg cctgccattg gacaagtata gagacgccat ctacgagcaa attgccgcgc tgcgccgttg ctccgcgtct ggcgagcaac ggcagcgcat ggagcgacaa gcttccagcg gtacccctag tcgcaagacg tccaccagca gcggcgccgc cgccgctgct gctgctacca gtggctctcg agcaggcacc agctcctcta caatcacgcg atccaacgcg agcagcagcg ctgccgtacg agttgtaccg cagcgcagca ctgtaaagtc tacttcgacc agtggcgtca acgattctgt accttcgaag acgtatgccc gtcccgcctt ccgccaggca gcgccttcca ccactgctat agaaagtaga cccgtcgcgc gcgagacagt ggtacgcaag tga
Details for LebrA.19448.a.HZ2.GE43706
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS NEIESHILKK YEIQTQLGQG AYGIVWRAVE RKYNRXVALK KIYDAFQNST DAQRTFREIM FLHRLHHPNI IKLLHVHRAY XDRDIYLVFX YMETDLHVVI RANILEEIHK QFIMYQLLKT MKYLHSAEIL HRDMKPSNLL INSDCTMKVA DFGLARSILS XESEQASRPV LTDYIATRWY RPPEILLGST RYTKGVDMWS VGCILGELML GKPIFPGRST TNQLELICSV TGMPSSADVA ATNSQFAHAM LRDIHLSHRR TFAELLPNAS ADALDLIQRL MCFNPNRRIS AKEALGHPYV AAFHRPDEEP VAPDPITISL PDNERLPLDK YRDAIYEQIA ALRRRSASGE QRQRM
Validated NT Sequence
ccatgcgctg ccgttgctcg ccagacgcgg agcgacggcg cagcgcggcg atttgctcgt agatggcgtc tctatacttg tccaatggca ggcgctcgtt gtcagggaga gagatggtga tgggatcggg cgccaccggc tcttcgtcag gacggtgaaa ggcagccacg tacgggtgtc ccagagcctc tttggcactg atgcgccggt ttgggttgaa gcacatcagg cgctggataa ggtccaacgc atccgcagaa gcgtttggca gaagctcggc gaatgtgcgt cggtgcgaga ggtggatgtc gcgaagcatg gcgtgggcaa actgcgagtt ggtcgccgcc acatccgccg aggaaggcat gcctgtcacg ctgcagataa gctctaactg gtttgtggtg gagcggccgg gaaagatggg cttgccaagc atcagctctc caagaatgca gcccaccgac cacatatcta cgcccttagt gtatcgtgtc gatccaagca gaatctccgg cggacggtac caccgcgtcg caatgtagtc tgttagcacc ggacgcgacg cctgctcact ctcnngtgaa aggatggagc gcgcgagacc gaagtcagcc accttcatcg tgcagtcgct gttgataagc aggttgcttg gcttcatgtc acggtgaaga atctcggcag agtgcaggta cttcatcgtc ttgagcagct ggtacatgat gaactgcttg tggatttctt ccaagatgtt tgcccgaatc accacgtgca ggtccgtctc catgtattng aagacaaggt agatatcgcg atcatngtag gcgcgatgga cgtgcagaag cttgatgatg ttggggtggt gcagccgatg caagaacata atctcacgga aggtgcgctg cgcatctgtc gagttttgga aagcgtcgta gatcttcttg agcgcaacga ngcggttgta tttgcgctcc acggcgcgcc aaacgatgcc gtatgcaccc tgtcctagct gcgtttgaat ctcatatttc tttagaatat gcgactcaat ctcgttcgac atcgaaccag gaccctgggt ctgagcttcc agggtaccac caatctgttc tctgtgagcc tcaataatat cgttatcctc catgtccaaa tcttcagggg tctgatcagc ttgaattcta ataccgtcgt acaagaatct taaggagtcc atttccttac cctgtctttt agcgaacgct tccatcagcc ttcttaaagg agtggtcttt ttgatcttga agaagatctc tgaagatcca tcggacacct ttaaattgat gtgagtctca ggcttgactt ctggcttgac ctctggctta gcttcttgat tgacttctga gtccgacttc atatgatggt gatggtgatg agccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMS NEIESHILKK YEIQTQLGQG AYGIVWRAVE RKYNRIVALK KIYDAFQNST DAQRTFREIM FLHRLHHPNI IKLLHVHRAY NDRDIYLVFE YMETDLHVVI RANILEEIHK QFIMYQLLKT MKYLHSAEIL HRDMKPSNLL INSDCTMKVA DFGLARSILS LESEQASRPV LTDYIATRWY RPPEILLGST RYTKGVDMWS VGCILGELML GKPIFPGRST TNQLELICSV TGMPSSADVA ATNSQFAHAM LRDIHLSHRR TFAELLPNAS ADALDLIQRL MCFNPNRRIS AKEALGHPYV AAFHRPDEEP VAPDPITISL PDNERLPLDK YRDAIYEQIA ALRRCSASGE QRQRMER
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tgtcgaacga gattgagtcg catattctaa agaaatatga gattcaaacg cagctaggac agggtgcata cggcatcgtt tggcgcgccg tggagcgcaa atacaaccgc atcgttgcgc tcaagaagat ctacgacgct tttcaaaact cgacagatgc gcagcgcacc ttccgtgaga ttatgttctt gcaccggctg caccacccca acatcatcaa gcttctgcac gtccatcgcg cctacaatga tcgcgatatc taccttgtct tcgaatacat ggagacggac ctgcacgtgg tgattcgggc aaacatcttg gaagaaattc acaagcagtt catcatgtac cagctgctca agacgatgaa gtacctgcac tctgccgaga ttcttcaccg tgacatgaag ccaagcaacc tgcttatcaa cagcgactgc acgatgaagg tggctgactt cggtctcgcg cgctccatcc tttcacttga gagtgagcag gcgtcgcgtc cggtgctaac agactacatt gcgacgcggt ggtaccgtcc gccggagatt ctgcttggat cgacacgata cactaagggc gtagatatgt ggtcggtggg ctgcattctt ggagagctga tgcttggcaa gcccatcttt cccggccgct ccaccacaaa ccagttagag cttatctgca gcgtgacagg catgccttcc tcggcggatg tggcggcgac caactcgcag tttgcccacg ccatgcttcg cgacatccac ctctcgcacc gacgcacatt cgccgaactt ctgccaaacg cttctgcgga tgcgttggac cttatccagc gcctgatgtg cttcaaccca aaccggcgca tcagtgcgaa agaggctctg ggacacccgt acgtggctgc ctttcaccgt cctgacgagg agccggtggc gcccgatccc atcaccatct ctctccctga caacgagcgc ctgccattgg acaagtatag agacgccatc tacgagcaaa ttgccgcgct gcgccgttgc tccgcgtctg gcgagcaacg gcagcgcatg gagcgataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa