SSGCID: Target Page

LebrA.19333.a

Contig, possible fusion of chromosomes 20 and 34 (EC 2.7.1.67) (Pi4K)

CENTER ID:	LebrA.19333.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.19333.a.B1.GE42067	full length		1	611

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.20.3170
UniProt:	A4HB77

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGDLAHRRMA AHRRSLLENL QRIFFTNQSA EKQEQCVRQL YDTNLHTLEE CLLQVTHVCI THQDPNAQEL LHNFMLWLAG RSLYIALRLA WIVDSVSTFD FSTGLAGRLK HFHDKLESYA INQGQAGTHS VSTSGGDGSW TSSDPEEGFS DVDASVVRRK EVRLKLYNDE RTFVTTLTNL SNMLRFFPDR TGRKDELRRG LRVLNQRLQS MWLVHPLCTS SESVQWIVNI SVEECTVFSS RERVPCLIRY EVIVDETATM RDPTATRLRL PDGRFRVRAD SDELFVPAPP PRSESPAASL VEESEGGAVE DASPEALQCL RAVFGELKVA RMARVRKSSP WGAHPNWCMN AMIIKAGEDL RQEELALQLI YAFQNIWQEA GLTVRVKPYA VLPTHRDCGL IEVIEDSSSM DGIKKATQVS SIYSYYLKAY DGEDSVLYRK AQQNFVESMA GYSIISYILQ IKDRHNGNLM IQRDGSLVHI DFGFLFVTSP GGLNFESAPF KLSQELIDVM GGVSSDLFNY YRILLYEALA AARERCEDLL ALVSILTPRN AMPCFGADPG AVVRQLRGRF REDLRSEADY AVYAKELILS SADNWRTRRY DQFQSLQNGI L

NT Sequence

atgggcgacc tagcacaccg acgcatggcg gcgcatcgcc gctcactgct ggagaatttg cagcggatct tcttcaccaa ccagtctgct gaaaagcagg aacagtgcgt ccgacagctc tatgacacga atctacacac tttggaggaa tgcctgctgc aagtcacgca tgtctgcatc acgcaccagg accccaacgc gcaggagctg ctgcacaact ttatgctctg gcttgccggc cggtcgctat acatcgcgct tcgccttgcg tggattgtgg attcagtgtc aacctttgat ttctctactg ggcttgccgg tcggctcaaa cactttcacg acaaacttga gagctacgcc atcaatcaag gacaggcagg cactcactcc gtcagcacca gcggcggtga cggcagctgg acctccagcg accccgagga ggggttcagc gacgtagatg ccagtgtcgt gcgacggaag gaagtgcgcc taaagctgta caacgacgaa cgtacgttcg tgactacgtt gactaacctg agcaacatgc tccgcttttt tcccgatcga accggccgca aggacgagct gcgcaggggt ctgcgagtgc tgaatcaacg actacagtcc atgtggctcg ttcacccact ctgtaccagc agtgaatctg tacagtggat tgtgaacatc tctgtcgaag agtgcaccgt cttctcgtcg cgcgagcggg tgccatgcct cattcgctac gaggtcatcg tagacgagac agcgactatg cgagacccaa cggcgacgcg gctccgactg cctgacgggc gctttcgtgt tcgggcggac tccgatgagc tctttgttcc agcaccgccg ccgaggtctg agtcgccggc cgcgtcgctg gtcgaagagt ctgagggcgg cgctgtggag gacgcctcgc cagaggcgct gcagtgtctg cgggctgtgt tcggcgagct caaagtggcg cgcatggcgc gagtgcgcaa gtcgtctcct tggggtgcgc accccaactg gtgcatgaac gcgatgatta tcaaggccgg agaagatctc cggcaggagg agctggcact gcagctcatc tacgctttcc aaaatatctg gcaggaggcg ggtctgacgg tgcgtgtgaa gccgtatgca gtgctaccta cacaccgtga ctgtggcctg attgaggtga tcgaggactc ttcgtctatg gatggtatca agaaggcgac ccaggtcagc agcatataca gctactacct caaggcctac gatggcgagg actccgtcct ctatcgcaaa gcgcaacaga actttgtgga gagcatggct ggctacagca tcatctcata catcctccaa attaaggacc gtcacaacgg caatcttatg attcaacgtg atggcagctt ggttcacatc gactttggct ttctgttcgt gacatcacca gggggcctga actttgaatc tgcgccgttt aaactgtcgc aggagctcat cgacgtcatg ggtggcgtct cgagcgacct ctttaactac tatcgaatcc ttctctacga ggcgctcgcg gcggcacgcg agcgctgcga ggacctgctt gcgttggtct ctatcttgac gccgaggaac gcgatgccgt gctttggggc agacccgggc gccgtcgttc gtcagctgcg cggacgcttt cgtgaggacc tgcgctccga ggcggactac gccgtgtacg caaaggagct catcctgagc agcgcggaca actggcgcac tcgacgctac gaccagtttc agagcctcca aaatgggatt ctttag

Details for LebrA.19333.a.B1.GE42067

HARVESTED ON:	12/5/2017
SEQUENCED ON:	12/7/2017
EXPECTED MW:	70kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG DLAHRRMAAH RRSLLENLQR IFFTNQSAEK QEQCVRQLYD TNLHTLEECL LQVTHVCITH QDPNAQELLH NFMLWLAGRS LYIALRLAWI VDSVSTFDFS TGLAGRLKHF HDKLESYAIN QGQAGTHSVS TSGGDGSWTS SDPEEGFSDV DASVVRRKEV RLKLYNDERT FVTTLTNLSN MLRFFPDRTG RKDELRRGLR VLNQRLQSMW LVHPLCTSSE SVQWIVNISV EECTVFSSRE RVPCLIRYEV IVDETATMRD PTATRLRLPD GRFRVRADSD ELFVPAPPPR SESPAASLVE ESEGGAVEDA SPEALQCLRA VFGELKVARM ARVRKSSPWG AHPNWCMNAM IIKAGEDLRQ EELALQLIYA FQNIWQEAGL TVRVKPYAVL PTHRDCGLIE VIEDSSSMDG IKKATQVSSI YSYYLKAYDG EDSVLYRKAQ QNFVESMAGY SIISYILQIK DRHNGNLMIQ RDGSLVHIDF GFLFVTSPGG LNFESAPFKL SQELIDVMGG VSSDLFNYYR ILLYEALAAA RERCEDLLAL VSILTPRNAM PCFGADPGAV VRQLRGRFRE DLRSEADYAV YAKELILSSA DNWRTRRYDQ FQSLQNGIL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg acctagcaca ccgacgcatg gcggcgcatc gccgctcact gctggagaat ttgcagcgga tcttcttcac caaccagtct gctgaaaagc aggaacagtg cgtccgacag ctctatgaca cgaatctaca cactttggag gaatgcctgc tgcaagtcac gcatgtctgc atcacgcacc aggaccccaa cgcgcaggag ctgctgcaca actttatgct ctggcttgcc ggccggtcgc tatacatcgc gcttcgcctt gcgtggattg tggattcagt gtcaaccttt gatttctcta ctgggcttgc cggtcggctc aaacactttc acgacaaact tgagagctac gccatcaatc aaggacaggc aggcactcac tccgtcagca ccagcggcgg tgacggcagc tggacctcca gcgaccccga ggaggggttc agcgacgtag atgccagtgt cgtgcgacgg aaggaagtgc gcctaaagct gtacaacgac gaacgtacgt tcgtgactac gttgactaac ctgagcaaca tgctccgctt ttttcccgat cgaaccggcc gcaaggacga gctgcgcagg ggtctgcgag tgctgaatca acgactacag tccatgtggc tcgttcaccc actctgtacc agcagtgaat ctgtacagtg gattgtgaac atctctgtcg aagagtgcac cgtcttctcg tcgcgcgagc gggtgccatg cctcattcgc tacgaggtca tcgtagacga gacagcgact atgcgagacc caacggcgac gcggctccga ctgcctgacg ggcgctttcg tgttcgggcg gactccgatg agctctttgt tccagcaccg ccgccgaggt ctgagtcgcc ggccgcgtcg ctggtcgaag agtctgaggg cggcgctgtg gaggacgcct cgccagaggc gctgcagtgt ctgcgggctg tgttcggcga gctcaaagtg gcgcgcatgg cgcgagtgcg caagtcgtct ccttggggtg cgcaccccaa ctggtgcatg aacgcgatga ttatcaaggc cggagaagat ctccggcagg aggagctggc actgcagctc atctacgctt tccaaaatat ctggcaggag gcgggtctga cggtgcgtgt gaagccgtat gcagtgctac ctacacaccg tgactgtggc ctgattgagg tgatcgagga ctcttcgtct atggatggta tcaagaaggc gacccaggtc agcagcatat acagctacta cctcaaggcc tacgatggcg aggactccgt cctctatcgc aaagcgcaac agaactttgt ggagagcatg gctggctaca gcatcatctc atacatcctc caaattaagg accgtcacaa cggcaatctt atgattcaac gtgatggcag cttggttcac atcgactttg gctttctgtt cgtgacatca ccagggggcc tgaactttga atctgcgccg tttaaactgt cgcaggagct catcgacgtc atgggtggcg tctcgagcga cctctttaac tactatcgaa tccttctcta cgaggcgctc gcggcggcac gcgagcgctg cgaggacctg cttgcgttgg tctctatctt gacgccgagg aacgcgatgc cgtgctttgg ggcagacccg ggcgccgtcg ttcgtcagct gcgcggacgc tttcgtgagg acctgcgctc cgaggcggac tacgccgtgt acgcaaagga gctcatcctg agcagcgcgg acaactggcg cactcgacgc tacgaccagt ttcagagcct ccaaaatggg attctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t