LebrA.18771.a

leucyl-tRNA synthetase, putative

CENTER ID: LebrA.18771.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.18771.a.B1.GE42668 full length 1 1075
LebrA.18771.a.B2.GE43046 XtalPred domain #1 42 1046
LebrA.18771.a.B3.GE43047 XtalPred domain #2 42 975
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.13.0900
UniProt: A4H796

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSTARRDTLV AIEQKKQADW AAKKLHEFDA PAPGEARAAK YLTTFPFPYM NGKLHLGHGF SLTKAEFASR FQRMMGRRSL WPFGFHVTGT PIAACAQKIA KEMQQYGNPP QFPSEVLEDK PKTPVDSKPT VALGQHKSKR GKSGPAKPQW VIMQSMGIPD SEIPKFADPQ HWLDYFPPIA IEDLKSFGCH IDWRRSFMTT ERNPYFDRFV QWQFHILRRE NLLNYGKRYC VYSPRDGQPC ADHDRASGEG VLPQEYTVVK LIVQNPLSQP CFAEHKDIIG DRNVILPGAT LRPETVIGQT NCWVSNKLNY KAYNVRNAKG EDEVYIMTAR AARNMAYQNF YVNGQTGVDP EPLFEVKGTD MVGMPLSAPL APYETIYTLP MSTITEAKGT GVVMSVPSDS PDDYINFSQL LNKPDYRAKL GIKDEWVVPF AMIPIIDIPE LGTEGAKFMC EKLKVNGPNA TELLEEAKKV CYQQGFYHGT MIVGPYTGVK VSEAKVKTQR DMEAADQCIR YYEPVRQVMS RSGDECVVAL CDQWYLEYGK DDWKRMVQDH VKKMDMFFPG VRNGFEETLN WLADWPCSRT FGLGTYLPCD ASHTMIIDSL SDSTIYMAYY TIDRFFNVGV DGSMDLCGKS DNPYGLTPEM FTDEVFEYIY HGVGDAATVA GAVSMPVESL KLMRNEFEYW YPVDLRCSGK DLIQNHLTMF LYNHAAIWPA DESKWPQRIF CNGHIQVDSE KMAKSKGNFI SLREAIDTYG ADATRLACAD AGDSMDDANF VRETAAGFVL KMTTLLEQAK ETVERADLRS GDYNEFDKIF QNTMNTIITR TEGYYHRMQF RMVLNTAFHE LSNEYSQYKM CCDDLNVHAH LGRRYYEVVA LMMMPLAPHT MEYLWQNILQ NEGSVVTQLF PKPTAELDYS LIVASRVMTT VIKEIRSQVI KNAKKRGPIE EVIVYTRREY MDWQRQALDI LQELYQANGN TFPEDMTKQV AARDPKIMSK GLMAFMSFVK ANVEKYGAQA MSTQPVVDDA AMLSNITHNL SRLSGVPVVR ILSAEDETYK EHDAARRKCL PGEPSVGFPP ASKSA
NT Sequence
atgtccacag cacgtcgcga tacgctcgtg gcgattgagc agaagaagca agcggactgg gccgccaaga agctgcatga gtttgatgcc ccggcgcctg gggaggcgcg ggcggcgaaa tacctgacga ccttcccctt cccgtacatg aacgggaaac ttcacctcgg ccacggcttc tcgctcacca aggccgagtt cgcctcgcgc tttcagcgca tgatgggccg ccgctcgctc tggccctttg gcttccatgt caccggcacc ccgattgccg cgtgcgcgca gaaaatcgcg aaggagatgc agcagtacgg caacccgccg cagtttccgt cagaggtgct ggaggacaag cccaagacac cggtggacag caagcctacg gtggcgctcg ggcagcacaa aagcaagcgc ggcaaaagtg ggccagcgaa gccgcagtgg gtcattatgc agagcatggg catccccgat tcggagatcc cgaagtttgc cgacccacag cactggttgg actacttccc gcccatcgct attgaagact tgaagagctt tggctgccac atcgactggc gccgttcctt tatgacgacg gagcgcaacc cgtactttga ccgctttgtc cagtggcagt tccacatcct gcgccgcgag aacctgctca actacggcaa gcgctactgc gtatattcgc cgcgcgacgg ccagccgtgc gccgaccacg atcgcgccag cggtgagggt gtgctgccgc aggaatacac ggtggtgaag ctcatcgtgc agaaccccct ctcgcagccg tgcttcgccg agcacaagga catcatcggt gaccgcaacg tcatcctgcc cggcgccacg ctccgcccgg aaacggtgat tggccagacg aactgctggg tgagcaacaa gctcaactac aaggcgtaca acgtgcgcaa cgcgaagggg gaggatgaag tgtacatcat gactgcccgc gccgcgcgca acatggcgta ccagaacttc tacgtgaacg gtcagaccgg cgtcgacccg gagccgctct tcgaagtgaa gggaacagac atggtcggca tgccgctttc ggcaccgctg gcgccttacg agacgatcta cacactgccc atgtccacca tcacggaggc gaagggtacg ggcgtcgtca tgtccgtgcc gtccgactcg cccgatgact acatcaactt ctcccagctg ctgaacaagc cagactaccg cgcgaagctc ggcatcaagg atgagtgggt agtcccgttc gccatgattc caatcatcga cattcccgag ctaggcactg aaggcgccaa gtttatgtgc gagaagctca aggtcaacgg cccgaacgcg acggagctgc tggaggaggc gaaaaaggta tgctaccagc aggggttcta ccatggcacc atgatcgtcg gtccctacac aggtgtcaag gtcagtgagg ccaaggtgaa gacgcagcgc gacatggagg cggcggacca gtgcatccgc tactacgagc cggtacgtca ggtgatgagc cgcagcggcg acgagtgcgt tgtcgcgctg tgcgatcagt ggtaccttga gtacggcaag gatgactgga agaggatggt acaggaccac gtcaagaaga tggacatgtt cttccccggt gtacgcaacg gcttcgagga gacgctgaac tggctagcag actggccgtg cagccgcacc ttcggcctcg gcacctacct accgtgcgac gcgtcgcaca cgatgatcat cgacagcctg tccgactcca ccatctacat ggcctactac acgatcgacc gcttcttcaa cgtcggcgtc gacggctcga tggacctgtg tggcaagtcg gacaacccgt acggcctcac gccagagatg ttcacagacg aggtgttcga gtacatatac cacggcgtcg gcgatgcggc caccgtcgcg ggtgcggtga gcatgccggt tgagtcgctg aagttgatgc gcaacgagtt cgagtactgg taccccgtcg acctgcgctg ctctggtaag gatctcatcc agaaccacct gacgatgttc ctctacaacc acgccgcgat ctggccggcg gacgagagca agtggcctca gaggatcttc tgcaacggtc acatccaggt cgacagcgag aagatggcca agtcaaaggg gaacttcatc tccctgcgcg aggcgatcga cacgtacggc gctgacgcca cacgcctcgc ctgtgctgac gccggtgaca gcatggacga cgccaacttt gtgcgcgaga cggcagctgg cttcgtgctg aagatgacga cactgctgga gcaggcgaag gagacagtag agcgcgccga tctgcgcagc ggcgactaca acgagtttga caagatcttc cagaacacaa tgaacacgat catcacccgt accgagggct actatcaccg catgcagttc cgcatggtgc tcaacacggc cttccacgag ctatcgaatg aatacagcca gtacaagatg tgctgcgacg acctcaacgt gcatgcacat ctcgggcgcc gctactacga ggtggtcgcg ctgatgatga tgccgctggc gccgcacacg atggagtatc tgtggcagaa catcctccag aacgagggtt ccgtcgtcac acagctgttc ccgaagccga cggcagagtt ggactactcg ctgatcgtcg caagccgggt catgacgact gttatcaagg aaattcggtc gcaggtgatc aagaacgcga agaagcgtgg tccaatcgag gaggtcattg tgtacacacg ccgcgagtac atggactggc agcggcaggc gctcgacatc ctgcaagagc tctaccaggc gaacggcaac acgtttcctg aagacatgac gaagcaggtt gccgcccgcg accccaagat catgagcaag ggtctgatgg ccttcatgtc cttcgtcaag gccaacgtag agaagtacgg ggcgcaggcc atgtcgacgc aacccgtcgt cgacgacgcc gcgatgctgt ccaacatcac gcacaacctc agccgcctct ccggggtgcc agtggtgcgc atcctgtccg ccgaagacga gacctacaag gagcacgatg cggcgcgccg caagtgcctg cctggtgagc cgtccgtggg cttcccaccg gcgtcgaaga gtgcgtag
Details for LebrA.18771.a.B1.GE42668
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 123kDa
OBSERVED MW: 122kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS TARRDTLVAI EQKKQADWAA KKLHEFDAPA PGEARAAKYL TTFPFPYMNG KLHLGHGFSL TKAEFASRFQ RMMGRRSLWP FGFHVTGTPI AACAQKIAKE MQQYGNPPQF PSEVLEDKPK TPVDSKPTVA LGQHKSKRGK SGPAKPQWVI MQSMGIPDSE IPKFADPQHW LDYFPPIAIE DLKSFGCHID WRRSFMTTER NPYFDRFVQW QFHILRRENL LNYGKRYCVY SPRDGQPCAD HDRASGEGVL PQEYTVVKLI VQNPLSQPCF AEHKDIIGDR NVILPGATLR PETVIGQTNC WVSNKLNYKA YNVRNAKGED EVYIMTARAA RNMAYQNFYV NGQTGVDPEP LFEVKGTDMV GMPLSAPLAP YETIYTLPMS TITEAKGTGV VMSVPSDSPD DYINFSQLLN KPDYRAKLGI KDEWVVPFAM IPIIDIPELG TEGAKFMCEK LKVNGPNATE LLEEAKKVCY QQGFYHGTMI VGPYTGVKVS EAKVKTQRDM EAADQCIRYY EPVRQVMSRS GDECVVALCD QWYLEYGKDD WKRMVQDHVK KMDMFFPGVR NGFEETLNWL ADWPCSRTFG LGTYLPCDAS HTMIIDSLSD STIYMAYYTI DRFFNVGVDG SMDLCGKSDN PYGLTPEMFT DEVFEYIYHG VGDAATVAGA VSMPVESLKL MRNEFEYWYP VDLRCSGKDL IQNHLTMFLY NHAAIWPADE SKWPQRIFCN GHIQVDSEKM AKSKGNFISL REAIDTYGAD ATRLACADAG DSMDDANFVR ETAAGFVLKM TTLLEQAKET VERADLRSGD YNEFDKIFQN TMNTIITRTE GYYHRMQFRM VLNTAFHELS NEYSQYKMCC DDLNVHAHLG RRYYEVVALM MMPLAPHTME YLWQNILQNE GSVVTQLFPK PTAELDYSLI VASRVMTTVI KEIRSQVIKN AKKRGPIEEV IVYTRREYMD WQRQALDILQ ELYQANGNTF PEDMTKQVAA RDPKIMSKGL MAFMSFVKAN VEKYGAQAMS TQPVVDDAAM LSNITHNLSR LSGVPVVRIL SAEDETYKEH DAARRKCLPG EPSVGFPPAS KSA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca cagcacgtcg cgatacgctc gtggcgattg agcagaagaa gcaagcggac tgggccgcca agaagctgca tgagtttgat gccccggcgc ctggggaggc gcgggcggcg aaatacctga cgaccttccc cttcccgtac atgaacggga aacttcacct cggccacggc ttctcgctca ccaaggccga gttcgcctcg cgctttcagc gcatgatggg ccgccgctcg ctctggccct ttggcttcca tgtcaccggc accccgattg ccgcgtgcgc gcagaaaatc gcgaaggaga tgcagcagta cggcaacccg ccgcagtttc cgtcagaggt gctggaggac aagcccaaga caccggtgga cagcaagcct acggtggcgc tcgggcagca caaaagcaag cgcggcaaaa gtgggccagc gaagccgcag tgggtcatta tgcagagcat gggcatcccc gattcggaga tcccgaagtt tgccgaccca cagcactggt tggactactt cccgcccatc gctattgaag acttgaagag ctttggctgc cacatcgact ggcgccgttc ctttatgacg acggagcgca acccgtactt tgaccgcttt gtccagtggc agttccacat cctgcgccgc gagaacctgc tcaactacgg caagcgctac tgcgtatatt cgccgcgcga cggccagccg tgcgccgacc acgatcgcgc cagcggtgag ggtgtgctgc cgcaggaata cacggtggtg aagctcatcg tgcagaaccc cctctcgcag ccgtgcttcg ccgagcacaa ggacatcatc ggtgaccgca acgtcatcct gcccggcgcc acgctccgcc cggaaacggt gattggccag acgaactgct gggtgagcaa caagctcaac tacaaggcgt acaacgtgcg caacgcgaag ggggaggatg aagtgtacat catgactgcc cgcgccgcgc gcaacatggc gtaccagaac ttctacgtga acggtcagac cggcgtcgac ccggagccgc tcttcgaagt gaagggaaca gacatggtcg gcatgccgct ttcggcaccg ctggcgcctt acgagacgat ctacacactg cccatgtcca ccatcacgga ggcgaagggt acgggcgtcg tcatgtccgt gccgtccgac tcgcccgatg actacatcaa cttctcccag ctgctgaaca agccagacta ccgcgcgaag ctcggcatca aggatgagtg ggtagtcccg ttcgccatga ttccaatcat cgacattccc gagctaggca ctgaaggcgc caagtttatg tgcgagaagc tcaaggtcaa cggcccgaac gcgacggagc tgctggagga ggcgaaaaag gtatgctacc agcaggggtt ctaccatggc accatgatcg tcggtcccta cacaggtgtc aaggtcagtg aggccaaggt gaagacgcag cgcgacatgg aggcggcgga ccagtgcatc cgctactacg agccggtacg tcaggtgatg agccgcagcg gcgacgagtg cgttgtcgcg ctgtgcgatc agtggtacct tgagtacggc aaggatgact ggaagaggat ggtacaggac cacgtcaaga agatggacat gttcttcccc ggtgtacgca acggcttcga ggagacgctg aactggctag cagactggcc gtgcagccgc accttcggcc tcggcaccta cctaccgtgc gacgcgtcgc acacgatgat catcgacagc ctgtccgact ccaccatcta catggcctac tacacgatcg accgcttctt caacgtcggc gtcgacggct cgatggacct gtgtggcaag tcggacaacc cgtacggcct cacgccagag atgttcacag acgaggtgtt cgagtacata taccacggcg tcggcgatgc ggccaccgtc gcgggtgcgg tgagcatgcc ggttgagtcg ctgaagttga tgcgcaacga gttcgagtac tggtaccccg tcgacctgcg ctgctctggt aaggatctca tccagaacca cctgacgatg ttcctctaca accacgccgc gatctggccg gcggacgaga gcaagtggcc tcagaggatc ttctgcaacg gtcacatcca ggtcgacagc gagaagatgg ccaagtcaaa ggggaacttc atctccctgc gcgaggcgat cgacacgtac ggcgctgacg ccacacgcct cgcctgtgct gacgccggtg acagcatgga cgacgccaac tttgtgcgcg agacggcagc tggcttcgtg ctgaagatga cgacactgct ggagcaggcg aaggagacag tagagcgcgc cgatctgcgc agcggcgact acaacgagtt tgacaagatc ttccagaaca caatgaacac gatcatcacc cgtaccgagg gctactatca ccgcatgcag ttccgcatgg tgctcaacac ggccttccac gagctatcga atgaatacag ccagtacaag atgtgctgcg acgacctcaa cgtgcatgca catctcgggc gccgctacta cgaggtggtc gcgctgatga tgatgccgct ggcgccgcac acgatggagt atctgtggca gaacatcctc cagaacgagg gttccgtcgt cacacagctg ttcccgaagc cgacggcaga gttggactac tcgctgatcg tcgcaagccg ggtcatgacg actgttatca aggaaattcg gtcgcaggtg atcaagaacg cgaagaagcg tggtccaatc gaggaggtca ttgtgtacac acgccgcgag tacatggact ggcagcggca ggcgctcgac atcctgcaag agctctacca ggcgaacggc aacacgtttc ctgaagacat gacgaagcag gttgccgccc gcgaccccaa gatcatgagc aagggtctga tggccttcat gtccttcgtc aaggccaacg tagagaagta cggggcgcag gccatgtcga cgcaacccgt cgtcgacgac gccgcgatgc tgtccaacat cacgcacaac ctcagccgcc tctccggggt gccagtggtg cgcatcctgt ccgccgaaga cgagacctac aaggagcacg atgcggcgcg ccgcaagtgc ctgcctggtg agccgtccgt gggcttccca ccggcgtcga agagtgcgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LebrA.18771.a.B2.GE43046
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 115kDa
OBSERVED MW: 115kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLT TFPFPYMNGK LHLGHGFSLT KAEFASRFQR MMGRRSLWPF GFHVTGTPIA ACAQKIAKEM QQYGNPPQFP SEVLEDKPKT PVDSKPTVAL GQHKSKRGKS GPAKPQWVIM QSMGIPDSEI PKFADPQHWL DYFPPIAIED LKSFGCHIDW RRSFMTTERN PYFDRFVQWQ FHILRRENLL NYGKRYCVYS PRDGQPCADH DRASGEGVLP QEYTVVKLIV QNPLSQPCFA EHKDIIGDRN VILPGATLRP ETVIGQTNCW VSNKLNYKAY NVRNAKGEDE VYIMTARAAR NMAYQNFYVN GQTGVDPEPL FEVKGTDMVG MPLSAPLAPY ETIYTLPMST ITEAKGTGVV MSVPSDSPDD YINFSQLLNK PDYRAKLGIK DEWVVPFAMI PIIDIPELGT EGAKFMCEKL KVNGPNATEL LEEAKKVCYQ QGFYHGTMIV GPYTGVKVSE AKVKTQRDME AADQCIRYYE PVRQVMSRSG DECVVALCDQ WYLEYGKDDW KRMVQDHVKK MDMFFPGVRN GFEETLNWLA DWPCSRTFGL GTYLPCDASH TMIIDSLSDS TIYMAYYTID RFFNVGVDGS MDLCGKSDNP YGLTPEMFTD EVFEYIYHGV GDAATVAGAV SMPVESLKLM RNEFEYWYPV DLRCSGKDLI QNHLTMFLYN HAAIWPADES KWPQRIFCNG HIQVDSEKMA KSKGNFISLR EAIDTYGADA TRLACADAGD SMDDANFVRE TAAGFVLKMT TLLEQAKETV ERADLRSGDY NEFDKIFQNT MNTIITRTEG YYHRMQFRMV LNTAFHELSN EYSQYKMCCD DLNVHAHLGR RYYEVVALMM MPLAPHTMEY LWQNILQNEG SVVTQLFPKP TAELDYSLIV ASRVMTTVIK EIRSQVIKNA KKRGPIEEVI VYTRREYMDW QRQALDILQE LYQANGNTFP EDMTKQVAAR DPKIMSKGLM AFMSFVKANV EKYGAQAMST QPVVDDAAML SNITHNLSRL SGVPVVRILS AED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgacga ccttcccctt cccgtacatg aacgggaaac ttcacctcgg ccacggcttc tcgctcacca aggccgagtt cgcctcgcgc tttcagcgca tgatgggccg ccgctcgctc tggccctttg gcttccatgt caccggcacc ccgattgccg cgtgcgcgca gaaaatcgcg aaggagatgc agcagtacgg caacccgccg cagtttccgt cagaggtgct ggaggacaag cccaagacac cggtggacag caagcctacg gtggcgctcg ggcagcacaa aagcaagcgc ggcaaaagtg ggccagcgaa gccgcagtgg gtcattatgc agagcatggg catccccgat tcggagatcc cgaagtttgc cgacccacag cactggttgg actacttccc gcccatcgct attgaagact tgaagagctt tggctgccac atcgactggc gccgttcctt tatgacgacg gagcgcaacc cgtactttga ccgctttgtc cagtggcagt tccacatcct gcgccgcgag aacctgctca actacggcaa gcgctactgc gtatattcgc cgcgcgacgg ccagccgtgc gccgaccacg atcgcgccag cggtgagggt gtgctgccgc aggaatacac ggtggtgaag ctcatcgtgc agaaccccct ctcgcagccg tgcttcgccg agcacaagga catcatcggt gaccgcaacg tcatcctgcc cggcgccacg ctccgcccgg aaacggtgat tggccagacg aactgctggg tgagcaacaa gctcaactac aaggcgtaca acgtgcgcaa cgcgaagggg gaggatgaag tgtacatcat gactgcccgc gccgcgcgca acatggcgta ccagaacttc tacgtgaacg gtcagaccgg cgtcgacccg gagccgctct tcgaagtgaa gggaacagac atggtcggca tgccgctttc ggcaccgctg gcgccttacg agacgatcta cacactgccc atgtccacca tcacggaggc gaagggtacg ggcgtcgtca tgtccgtgcc gtccgactcg cccgatgact acatcaactt ctcccagctg ctgaacaagc cagactaccg cgcgaagctc ggcatcaagg atgagtgggt agtcccgttc gccatgattc caatcatcga cattcccgag ctaggcactg aaggcgccaa gtttatgtgc gagaagctca aggtcaacgg cccgaacgcg acggagctgc tggaggaggc gaaaaaggta tgctaccagc aggggttcta ccatggcacc atgatcgtcg gtccctacac aggtgtcaag gtcagtgagg ccaaggtgaa gacgcagcgc gacatggagg cggcggacca gtgcatccgc tactacgagc cggtacgtca ggtgatgagc cgcagcggcg acgagtgcgt tgtcgcgctg tgcgatcagt ggtaccttga gtacggcaag gatgactgga agaggatggt acaggaccac gtcaagaaga tggacatgtt cttccccggt gtacgcaacg gcttcgagga gacgctgaac tggctagcag actggccgtg cagccgcacc ttcggcctcg gcacctacct accgtgcgac gcgtcgcaca cgatgatcat cgacagcctg tccgactcca ccatctacat ggcctactac acgatcgacc gcttcttcaa cgtcggcgtc gacggctcga tggacctgtg tggcaagtcg gacaacccgt acggcctcac gccagagatg ttcacagacg aggtgttcga gtacatatac cacggcgtcg gcgatgcggc caccgtcgcg ggtgcggtga gcatgccggt tgagtcgctg aagttgatgc gcaacgagtt cgagtactgg taccccgtcg acctgcgctg ctctggtaag gatctcatcc agaaccacct gacgatgttc ctctacaacc acgccgcgat ctggccggcg gacgagagca agtggcctca gaggatcttc tgcaacggtc acatccaggt cgacagcgag aagatggcca agtcaaaggg gaacttcatc tccctgcgcg aggcgatcga cacgtacggc gctgacgcca cacgcctcgc ctgtgctgac gccggtgaca gcatggacga cgccaacttt gtgcgcgaga cggcagctgg cttcgtgctg aagatgacga cactgctgga gcaggcgaag gagacagtag agcgcgccga tctgcgcagc ggcgactaca acgagtttga caagatcttc cagaacacaa tgaacacgat catcacccgt accgagggct actatcaccg catgcagttc cgcatggtgc tcaacacggc cttccacgag ctatcgaatg aatacagcca gtacaagatg tgctgcgacg acctcaacgt gcatgcacat ctcgggcgcc gctactacga ggtggtcgcg ctgatgatga tgccgctggc gccgcacacg atggagtatc tgtggcagaa catcctccag aacgagggtt ccgtcgtcac acagctgttc ccgaagccga cggcagagtt ggactactcg ctgatcgtcg caagccgggt catgacgact gttatcaagg aaattcggtc gcaggtgatc aagaacgcga agaagcgtgg tccaatcgag gaggtcattg tgtacacacg ccgcgagtac atggactggc agcggcaggc gctcgacatc ctgcaagagc tctaccaggc gaacggcaac acgtttcctg aagacatgac gaagcaggtt gccgcccgcg accccaagat catgagcaag ggtctgatgg ccttcatgtc cttcgtcaag gccaacgtag agaagtacgg ggcgcaggcc atgtcgacgc aacccgtcgt cgacgacgcc gcgatgctgt ccaacatcac gcacaacctc agccgcctct ccggggtgcc agtggtgcgc atcctgtccg ccgaagactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for LebrA.18771.a.B3.GE43047
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 108kDa
OBSERVED MW: 108kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHLT TFPFPYMNGK LHLGHGFSLT KAEFASRFQR MMGRRSLWPF GFHVTGTPIA ACAQKIAKEM QQYGNPPQFP SEVLEDKPKT PVDSKPTVAL GQHKSKRGKS GPAKPQWVIM QSMGIPDSEI PKFADPQHWL DYFPPIAIED LKSFGCHIDW RRSFMTTERN PYFDRFVQWQ FHILRRENLL NYGKRYCVYS PRDGQPCADH DRASGEGVLP QEYTVVKLIV QNPLSQPCFA EHKDIIGDRN VILPGATLRP ETVIGQTNCW VSNKLNYKAY NVRNAKGEDE VYIMTARAAR NMAYQNFYVN GQTGVDPEPL FEVKGTDMVG MPLSAPLAPY ETIYTLPMST ITEAKGTGVV MSVPSDSPDD YINFSQLLNK PDYRAKLGIK DEWVVPFAMI PIIDIPELGT EGAKFMCEKL KVNGPNATEL LEEAKKVCYQ QGFYHGTMIV GPYTGVKVSE AKVKTQRDME AADQCIRYYE PVRQVMSRSG DECVVALCDQ WYLEYGKDDW KRMVQDHVKK MDMFFPGVRN GFEETLNWLA DWPCSRTFGL GTYLPCDASH TMIIDSLSDS TIYMAYYTID RFFNVGVDGS MDLCGKSDNP YGLTPEMFTD EVFEYIYHGV GDAATVAGAV SMPVESLKLM RNEFEYWYPV DLRCSGKDLI QNHLTMFLYN HAAIWPADES KWPQRIFCNG HIQVDSEKMA KSKGNFISLR EAIDTYGADA TRLACADAGD SMDDANFVRE TAAGFVLKMT TLLEQAKETV ERADLRSGDY NEFDKIFQNT MNTIITRTEG YYHRMQFRMV LNTAFHELSN EYSQYKMCCD DLNVHAHLGR RYYEVVALMM MPLAPHTMEY LWQNILQNEG SVVTQLFPKP TAELDYSLIV ASRVMTTVIK EIRSQVIKNA KKRGPIEEVI VYTRREYMDW QRQALDILQE LYQANGNTFP ED
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catctgacga ccttcccctt cccgtacatg aacgggaaac ttcacctcgg ccacggcttc tcgctcacca aggccgagtt cgcctcgcgc tttcagcgca tgatgggccg ccgctcgctc tggccctttg gcttccatgt caccggcacc ccgattgccg cgtgcgcgca gaaaatcgcg aaggagatgc agcagtacgg caacccgccg cagtttccgt cagaggtgct ggaggacaag cccaagacac cggtggacag caagcctacg gtggcgctcg ggcagcacaa aagcaagcgc ggcaaaagtg ggccagcgaa gccgcagtgg gtcattatgc agagcatggg catccccgat tcggagatcc cgaagtttgc cgacccacag cactggttgg actacttccc gcccatcgct attgaagact tgaagagctt tggctgccac atcgactggc gccgttcctt tatgacgacg gagcgcaacc cgtactttga ccgctttgtc cagtggcagt tccacatcct gcgccgcgag aacctgctca actacggcaa gcgctactgc gtatattcgc cgcgcgacgg ccagccgtgc gccgaccacg atcgcgccag cggtgagggt gtgctgccgc aggaatacac ggtggtgaag ctcatcgtgc agaaccccct ctcgcagccg tgcttcgccg agcacaagga catcatcggt gaccgcaacg tcatcctgcc cggcgccacg ctccgcccgg aaacggtgat tggccagacg aactgctggg tgagcaacaa gctcaactac aaggcgtaca acgtgcgcaa cgcgaagggg gaggatgaag tgtacatcat gactgcccgc gccgcgcgca acatggcgta ccagaacttc tacgtgaacg gtcagaccgg cgtcgacccg gagccgctct tcgaagtgaa gggaacagac atggtcggca tgccgctttc ggcaccgctg gcgccttacg agacgatcta cacactgccc atgtccacca tcacggaggc gaagggtacg ggcgtcgtca tgtccgtgcc gtccgactcg cccgatgact acatcaactt ctcccagctg ctgaacaagc cagactaccg cgcgaagctc ggcatcaagg atgagtgggt agtcccgttc gccatgattc caatcatcga cattcccgag ctaggcactg aaggcgccaa gtttatgtgc gagaagctca aggtcaacgg cccgaacgcg acggagctgc tggaggaggc gaaaaaggta tgctaccagc aggggttcta ccatggcacc atgatcgtcg gtccctacac aggtgtcaag gtcagtgagg ccaaggtgaa gacgcagcgc gacatggagg cggcggacca gtgcatccgc tactacgagc cggtacgtca ggtgatgagc cgcagcggcg acgagtgcgt tgtcgcgctg tgcgatcagt ggtaccttga gtacggcaag gatgactgga agaggatggt acaggaccac gtcaagaaga tggacatgtt cttccccggt gtacgcaacg gcttcgagga gacgctgaac tggctagcag actggccgtg cagccgcacc ttcggcctcg gcacctacct accgtgcgac gcgtcgcaca cgatgatcat cgacagcctg tccgactcca ccatctacat ggcctactac acgatcgacc gcttcttcaa cgtcggcgtc gacggctcga tggacctgtg tggcaagtcg gacaacccgt acggcctcac gccagagatg ttcacagacg aggtgttcga gtacatatac cacggcgtcg gcgatgcggc caccgtcgcg ggtgcggtga gcatgccggt tgagtcgctg aagttgatgc gcaacgagtt cgagtactgg taccccgtcg acctgcgctg ctctggtaag gatctcatcc agaaccacct gacgatgttc ctctacaacc acgccgcgat ctggccggcg gacgagagca agtggcctca gaggatcttc tgcaacggtc acatccaggt cgacagcgag aagatggcca agtcaaaggg gaacttcatc tccctgcgcg aggcgatcga cacgtacggc gctgacgcca cacgcctcgc ctgtgctgac gccggtgaca gcatggacga cgccaacttt gtgcgcgaga cggcagctgg cttcgtgctg aagatgacga cactgctgga gcaggcgaag gagacagtag agcgcgccga tctgcgcagc ggcgactaca acgagtttga caagatcttc cagaacacaa tgaacacgat catcacccgt accgagggct actatcaccg catgcagttc cgcatggtgc tcaacacggc cttccacgag ctatcgaatg aatacagcca gtacaagatg tgctgcgacg acctcaacgt gcatgcacat ctcgggcgcc gctactacga ggtggtcgcg ctgatgatga tgccgctggc gccgcacacg atggagtatc tgtggcagaa catcctccag aacgagggtt ccgtcgtcac acagctgttc ccgaagccga cggcagagtt ggactactcg ctgatcgtcg caagccgggt catgacgact gttatcaagg aaattcggtc gcaggtgatc aagaacgcga agaagcgtgg tccaatcgag gaggtcattg tgtacacacg ccgcgagtac atggactggc agcggcaggc gctcgacatc ctgcaagagc tctaccaggc gaacggcaac acgtttcctg aagactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat