SSGCID: Target Page

LebrA.18708.a

isoleucyl-tRNA synthetase, putative

CENTER ID:	LebrA.18708.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.18708.a.B2.GE42667	soluble domain #1		1	606

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.35.5900
UniProt:	A4HQ78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAGESTQQHE DPQAGQQRGP LKNFPDVLNF SHMEEEVLAM WREKDCFRTS MKLSEGRRPF SFYDGPPFAT GLPHYGHILA GTIKDMVTRF AYQTNHHVVR RFGWDCHGLP VEYEIDKMLG IKTSHDVAKL GIDKYNAECK KIVTRYVDEW RKTVERVGRW IDFDDDYKTM HLTYMESVWW VFSQLWEKKL VYRGFRVMPY STACTTPLSN FEANSNYKEV SDLAVTIAFQ TRDDPNTYFL AWTTTPWTLP SNLSLCVHPE LDYVKVLDTK SKRHYWFAEA RLAEVYPKKE GKKANKAKDG DNAAEADAAP YAVVEKLKGA QLVGARYVPL FSYFESSMGA TAFRVISGTY VTTDAGTGIV HQAPAFGEDD YQVCLDAGIF EKGGKFVCPV DENGMFTGEV TDFAGKHVKE ADPEIIKALE AKGHLFHKAS IVHSYPFCWR SDTPLIYKAV ESWFVNVEAF RDRLIDCNDK TYWVPDFVKT RRFSNWLAEA RDWNVSRNRY WGTPLPIWHS EDWEEIVCIG SVKQLEELSG VTGITDIHRD FVDHITIPSK RPGMPRLKRV PMVFDCWFES GSMPYAQEHY PYENQDKFKS LFPADFVSEG LDQTRGWFYT LLVLGVALFD VSPFRNVAVS GLVLAEDGKK MSKRLKNYPE PKVVVDTYGA DALRMYMISS PVVRAEPLRF RETGVKAVVK DILLPLFNAA KFFISNTNYC IEAGGQVSLQ VRSTNEMDRW ILASCQTLLR YVKAEMKLYR LYNVVPGILR FVGDLSNWYV RMNRRRMKNS TDSEDRSQAL STMLYLLFSV SRIVGHIAPF VAEMLYQQIK PLLPVTTQVD SVHYLMIPDE DASYDDPELE RAMSRMMNIV DLVRVLRDQM VIPIKRPVRQ VVIVHPDQEY LDDVRKVANY IKDEVNAFEI VMSSGQEYLE TKLDANFEVL GKKYRKEMPA IRKGIQAMTP EEVANFLHDQ KGVVVGKELT IEDVKVLRQV KEGITDFQSN TDNDVVVLVD KREDQELIDS WRAREFVNRV QQLRKKAKLL VTDRVDVYFE AEEADLTASI LQSKDQINQT LRGMWTTMDQ KPSDATLVAE EDNSISGMAV RLVFTNPKA

NT Sequence

atggcgggtg agagtacgca gcagcacgaa gacccccaag cgggacagca acgtgggccg ctgaagaatt tccccgatgt gcttaatttc tcgcacatgg aggaggaggt gctggcaatg tggcgcgaga aagactgctt tcgcacctca atgaagctct ccgagggccg caggcccttt agcttctacg acggcccgcc gtttgcgact ggtctcccgc actacgggca tatcttggct ggcactatca aggacatggt cactcgcttt gcctatcaga cgaaccacca cgtcgtccgt cgctttggct gggactgcca cggcctcccg gtggagtacg agattgacaa gatgctcggg atcaagacgt cgcatgacgt ggcgaagctc ggcattgaca aatacaacgc cgagtgcaag aagattgtca cccgttacgt tgacgagtgg aggaagacgg tcgagcgcgt cggccgctgg atcgacttcg acgacgacta caagacgatg cacctcacgt acatggagag tgtctggtgg gtcttctcgc agctatggga aaagaagctg gtgtatcgcg gcttccgcgt gatgccatac tcgaccgcct gtacgacgcc gctgagcaac ttcgaggcga actccaacta caaggaggtc agcgacctgg ctgtgacgat tgccttccag actcgcgacg accccaacac gtacttcctt gcgtggacta caacgccatg gactttgccg agcaacctca gtttgtgcgt gcaccccgag ttggactacg tgaaggtgtt ggacaccaag tcgaagcggc actactggtt cgcggaggcc cgcctggcgg aggtgtaccc gaagaaggag ggcaagaaag ccaacaaggc aaaggatggc gacaacgctg cggaggccga tgccgcaccg tatgcggtcg tggagaagct caagggcgca cagctcgtgg gtgcgagata cgtaccgctt ttctcgtact ttgagtcgtc catgggtgca acggccttcc gtgtcatctc cggtacctac gtgacgacgg acgctggtac ggggattgtg catcaggccc ccgccttcgg tgaagatgat taccaggtgt gcctcgacgc cggcatcttt gagaagggtg gcaagttcgt ctgcccggtc gacgagaacg gcatgttcac gggtgaggta acagacttcg ccggcaagca cgtcaaggag gcggacccgg agattatcaa ggcgcttgag gcgaaggggc atctcttcca caaggcgtcc attgtgcaca gctatccctt ttgttggcgc agcgacaccc cgctcatcta caaggctgtc gagtcgtggt ttgtcaacgt cgaggccttc cgcgaccgtc tcatcgactg caacgacaag acgtactggg tgcctgactt cgtcaagacc cgccgctttt ccaactggct cgccgaggcc cgcgactgga acgtgtcgcg caaccgctac tggggaacac cactgccgat ctggcacagc gaggactggg aggagattgt gtgcatcggc agtgtcaagc agctcgagga gctctccggc gtcaccggca tcacagacat ccaccgcgat ttcgtcgacc atatcacgat ccccagtaag cgtcccggca tgccgcggtt gaagcgcgtg ccgatggtat tcgattgctg gtttgagtct ggctcgatgc cgtatgccca ggagcactac ccctatgaga accaggataa gttcaagagc ctgtttccgg ccgactttgt ctcggagggc ttagaccaga cgcgcggttg gttctacacc ctcctggtgc tcggcgttgc cctcttcgac gtgtcccctt tccgcaacgt cgccgtcagc gggctcgttc tcgccgagga cggtaagaaa atgagcaagc gcctcaagaa ctacccggag ccgaaggtgg tggtcgacac atacggcgcg gatgcactgc gcatgtacat gattagttct ccggtggtgc gcgccgagcc gctgcgcttc cgcgagactg gtgtgaaggc tgtggtcaag gacatcctcc tgcccctgtt caacgccgcg aagttcttca tctcgaacac gaactactgc attgaggccg gtggtcaggt gtcgctgcag gtgcgcagca cgaacgagat ggaccgctgg atcctcgcct cgtgccagac tctactgcgc tacgtgaagg ccgagatgaa gctgtaccgc ctgtacaacg tcgttcccgg cattctgcgc ttcgtcggtg acttgtccaa ttggtacgtg cgcatgaacc gccgccgcat gaagaactcc accgacagcg aggaccgctc gcaggcactg tcgacaatgt tgtatctcct cttttcggtc tcccgcattg tgggccacat cgcccccttt gtggcagaaa tgctttatca gcagatcaaa cccctcctcc cggtgaccac acaagtggac tctgtacact acctcatgat tcccgacgag gacgcctcct acgacgaccc ggaactggag cgcgccatgt cgcgcatgat gaacattgtg gatctggtcc gtgtgctgcg cgatcagatg gtcatcccta tcaaacggcc agtgcgacag gttgttattg tgcacccgga ccaagagtac cttgatgacg tgcgcaaagt ggcgaactac atcaaggacg aggtgaacgc attcgagatt gtcatgtcga gtggccagga gtacctggag accaaactgg acgcgaactt cgaggtgctt ggcaagaagt accgcaagga gatgcctgcg atccgcaaag gcatccaggc catgacaccg gaggaggtgg ccaacttcct gcatgaccaa aagggcgtcg tcgtcggcaa ggagctgacc atcgaggacg tcaaggtact gcgccaggtc aaggagggca tcaccgactt ccagagcaac acggacaacg atgtcgtcgt gctggtcgac aagcgcgagg atcaggaact cattgactcg tggcgcgcac gtgagttcgt taaccgtgta cagcagctgc ggaagaaggc gaagctgctg gtgacggacc gggtggatgt gtacttcgaa gcggaggagg cagacctgac agcctccatc ctccagtcca aggaccagat caaccagacg ttgaggggta tgtggacaac gatggaccag aagcccagcg acgccacgct tgtcgcggag gaagataaca gcatctcggg catggcggtg cgcctcgtct tcaccaatcc caaagcttaa

Details for LebrA.18708.a.B2.GE42667

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	73
PERCENT COVERAGE:	58

Validated AA Sequence

MAHHHHHHMA GESTQPHEDP QAGQQRGPLK NYPDVLNFSH MEEEVLTMWR EKDCFRTSMK LSEGRRPFSF YDGPPFATGL PHYGHILAGT IKDMVTRFAY QTNHHVVRRF GWDCHGLPVE YEIDKMLGIK TSHDVAKLGI DKYNAECKKI VTRYVDEWRK TVERVGRWID FDNDYKTMHL TYMESVWWVF SQLWEKKLVY RGFRVMPYST ACTTPLSNFE ANSNYKEVSD LAVTIAFQTR DDPNTYFLAW TXXXXTLPSX LXXCXHPXLD XXXVXXXXSX XXXXXXXXXL XXXXXXXXXX XXXXXXRXXX XXXXXXXXXX XXXXXXXXHX XXXXXXYXXX XTXXRPWVQX XXVSSPVPT

Validated NT Sequence

tccgagacaa agtcggccgg aaacaggctc ttgaacttat cctggttctc atacgggtag tgttcctggg catacggcat cgagccagac tcaaaccagc aatcgaatac catcggcaca cgcttcaacc gcggcatgcc gggacgctta ctggggatcg tgatatggtc gacgaaatcc cggtggatgt ctgtgatgcc ggtgacgccg gagagctcct cgagctgctt gacactgccg atgcacacaa tctcctccca gtcctcgctg tgccagattg gcagtggtgt tccccagtag cggttgcgcg acacgttcca gtcgcgggcc tcggcgagcc agttggaaaa gcggcgggtc ttgacgaagt caggcaccca gtacgtcttg tcgttgcagt cgatgagacg gtcgcggaag gcctcgacgt tgacaaacca cgactcgaca gccttgtaga tgagcggggt gtcgctgcgc caacagaagg gatagctgtg cacaatggac gccttgtgga agagatgccc cttcgcctca agcgccttga taatctccgg gtccgcctcc ttgacgtgct tgccggcgaa gtctgttacc tcacccgtga acatgccgtt ctcgtcgacc gggcagacga acttgccacc cttctcaaag atgccggcgt cgaggcacac ctggtaatca tcctcaccga aggcgggggc ctgatgcaca atccccgtac cagcgtccgt cgtcacgtag gtaccggaga tgacacggna nnnnnttgca cccatggacg acncnaagta ngnnnnnnnn ggtacnnnnn tnnnnccnnn gnnntgtgnn nnnntgnnnn ncnnnnnnnc ngcannnnnn nnnncnnnnn nnnnnnnnnn nnnnnnnnnn nccttnnnnn nngnnnnntt ncnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagnnnnn nnnnnnnnnn nnnnnnnnnn nnnttcgacn nnnnnnnnnn caccnnnnnn tagtccaacn nngggtgnnn gcacnnnnnn aggnngctcg gcaaagtnnn nnncnnnnta gtccacgcaa ggaagtacgt gttggggtcg tcgcgagtct ggaaggcaat cgtcacagcc aggtcgctga cctccttgta gttggagttc gcctcgaagt tgctcagcgg cgtcgtacag gcggtcgagt atggcatcac gcggaagccg cgatacacca gcttcttttc ccatagctgc gagaagaccc accagacact ctccatgtac gtgaggtgca tcgtcttgta gtcgttgtcg aagtcgatcc agcggccgac gcgctcgacc gtcttcctcc actcgtcaac gtaacgggtg acaatcttct tgcactcggc gttgtatttg tcaatgccga gcttcgccac gtcatgcgac gtcttgatcc cgagcatctt gtcaatctcg tactccaccg ggaggccgtg gcagtcccag ccaaagcgac ggacgacgtg gtggttcgtc tgataggcaa agcgagtgac catgtccttg atagtgccag ccaagatatg cccgtagtgc gggagaccag tcgcaaacgg cgggccgtcg tagaagctaa agggcctgcg gccctcggag agcttcattg aggtgcgaaa gcagtctttc tcgcgccaca ttgtcagcac ctcctcctcc atgtgcgaga aattaagcac atcggggtaa ttcttcagcg gcccacgttg ctgtcccgct tgggggtctt cgtgcggctg cgtactctca cccgccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMA GESTQQHEDP QAGQQRGPLK NFPDVLNFSH MEEEVLAMWR EKDCFRTSMK LSEGRRPFSF YDGPPFATGL PHYGHILAGT IKDMVTRFAY QTNHHVVRRF GWDCHGLPVE YEIDKMLGIK TSHDVAKLGI DKYNAECKKI VTRYVDEWRK TVERVGRWID FDDDYKTMHL TYMESVWWVF SQLWEKKLVY RGFRVMPYST ACTTPLSNFE ANSNYKEVSD LAVTIAFQTR DDPNTYFLAW TTTPWTLPSN LSLCVHPELD YVKVLDTKSK RHYWFAEARL AEVYPKKEGK KANKAKDGDN AAEADAAPYA VVEKLKGAQL VGARYVPLFS YFESSMGATA FRVISGTYVT TDAGTGIVHQ APAFGEDDYQ VCLDAGIFEK GGKFVCPVDE NGMFTGEVTD FAGKHVKEAD PEIIKALEAK GHLFHKASIV HSYPFCWRSD TPLIYKAVES WFVNVEAFRD RLIDCNDKTY WVPDFVKTRR FSNWLAEARD WNVSRNRYWG TPLPIWHSED WEEIVCIGSV KQLEELSGVT GITDIHRDFV DHITIPSKRP GMPRLKRVPM VFDCWFESGS MPYAQEHYPY ENQDKFKSLF PADFVSEGLD QTRG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgg gtgagagtac gcagcagcac gaagaccccc aagcgggaca gcaacgtggg ccgctgaaga atttccccga tgtgcttaat ttctcgcaca tggaggagga ggtgctggca atgtggcgcg agaaagactg ctttcgcacc tcaatgaagc tctccgaggg ccgcaggccc tttagcttct acgacggccc gccgtttgcg actggtctcc cgcactacgg gcatatcttg gctggcacta tcaaggacat ggtcactcgc tttgcctatc agacgaacca ccacgtcgtc cgtcgctttg gctgggactg ccacggcctc ccggtggagt acgagattga caagatgctc gggatcaaga cgtcgcatga cgtggcgaag ctcggcattg acaaatacaa cgccgagtgc aagaagattg tcacccgtta cgttgacgag tggaggaaga cggtcgagcg cgtcggccgc tggatcgact tcgacgacga ctacaagacg atgcacctca cgtacatgga gagtgtctgg tgggtcttct cgcagctatg ggaaaagaag ctggtgtatc gcggcttccg cgtgatgcca tactcgaccg cctgtacgac gccgctgagc aacttcgagg cgaactccaa ctacaaggag gtcagcgacc tggctgtgac gattgccttc cagactcgcg acgaccccaa cacgtacttc cttgcgtgga ctacaacgcc atggactttg ccgagcaacc tcagtttgtg cgtgcacccc gagttggact acgtgaaggt gttggacacc aagtcgaagc ggcactactg gttcgcggag gcccgcctgg cggaggtgta cccgaagaag gagggcaaga aagccaacaa ggcaaaggat ggcgacaacg ctgcggaggc cgatgccgca ccgtatgcgg tcgtggagaa gctcaagggc gcacagctcg tgggtgcgag atacgtaccg cttttctcgt actttgagtc gtccatgggt gcaacggcct tccgtgtcat ctccggtacc tacgtgacga cggacgctgg tacggggatt gtgcatcagg cccccgcctt cggtgaagat gattaccagg tgtgcctcga cgccggcatc tttgagaagg gtggcaagtt cgtctgcccg gtcgacgaga acggcatgtt cacgggtgag gtaacagact tcgccggcaa gcacgtcaag gaggcggacc cggagattat caaggcgctt gaggcgaagg ggcatctctt ccacaaggcg tccattgtgc acagctatcc cttttgttgg cgcagcgaca ccccgctcat ctacaaggct gtcgagtcgt ggtttgtcaa cgtcgaggcc ttccgcgacc gtctcatcga ctgcaacgac aagacgtact gggtgcctga cttcgtcaag acccgccgct tttccaactg gctcgccgag gcccgcgact ggaacgtgtc gcgcaaccgc tactggggaa caccactgcc gatctggcac agcgaggact gggaggagat tgtgtgcatc ggcagtgtca agcagctcga ggagctctcc ggcgtcaccg gcatcacaga catccaccgc gatttcgtcg accatatcac gatccccagt aagcgtcccg gcatgccgcg gttgaagcgc gtgccgatgg tattcgattg ctggtttgag tctggctcga tgccgtatgc ccaggagcac tacccctatg agaaccagga taagttcaag agcctgtttc cggccgactt tgtctcggag ggcttagacc agacgcgcgg ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat