SSGCID: Target Page

LebrA.18681.a

prolyl-tRNA synthetase, putative

CENTER ID:	LebrA.18681.a
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.18681.a.B1.GE42666	full length		1	719

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.18.1310
UniProt:	A4H9N1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSIEDCRGFD EVKALFQELG LDIPILHHDE KATVEEVLDL LKEKMGITAV GTKTLFLKSK KGDLVMATAL KSTATELKFI QNVTNTKDLR FASNEVLHEC LAVVQGCVTP FPLLNHTENR PITLLLDTSM AHSTQLLAFC LCRNDYTAAI TFQQLKMYLE KIGYAYKLVD FGAASADAAV TDSTAAPAAK PKRAAVAAAT PAPAPSGETR LGIAARREEN FSAWYIDVIT KAEMVEYYDV SGCYIIRPWA YYVWKCVQRF LGGRIERLGV EDCYFPMFVS RSCLEREKDH IEGFAPEVAW VTRAGDTELE QPVAVRPTSE TVMYPYYAKW IRSHRDLPVR LNMWNNVIRW EFSHPTPFIR TREFLWQEGH CAWAKAEDCA KEVLDILECY AAVYEQLLAV PVVRGRKTEK EKFAGGSYTT TVETFIEAVG RGCQGATSHN LGQNFGKMFD IRFQDPENNE QTLIPWQNSW GLSTRVIGVM IMVHGDNRGM VMPPRVASTQ VIIVPVGITK DTTEEARQAL LASCRQLEGE LCEGGVRAKC DLRDNYSPGW RFNHWEVKGV PLRVELGPRE LAERSLVVAV RHSCAKQSLT WNAQTPTAVA ALLEDVQAQM YARAKETMET HRVRVTEWAE FVPALNRKCL ILAPWCGAIE CEDQVRKDSA EESRAAQAQE VREDARAPSM GAKTLCIPFS QPEPAESHTC ICKGCTKAAT TWVLFGRSY

NT Sequence

atgtccatcg aggactgccg cggctttgat gaggtgaagg ccctcttcca agagctaggg cttgacattc ccattctgca ccatgacgag aaagccaccg tcgaggaggt gctggacctt ctgaaggaga agatgggcat caccgcggtg ggcaccaaga cactcttcct caagagcaaa aaaggcgacc tggtgatggc gacggcgctc aagtcaaccg cgacagaact caagttcatt caaaacgtta ctaacaccaa ggacctgcgc tttgcctcca acgaggtgtt gcatgagtgc ctcgccgtcg tgcagggatg cgtcacgccg ttcccactcc tcaaccacac agaaaaccgt cccattacgc tcctactgga cacctcaatg gcgcacagca cgcagctgct cgccttttgt ctgtgccgta acgattacac cgccgctatc acattccagc agctcaagat gtacctcgag aagatcgggt acgcgtacaa actggtggac tttggtgctg cctcggcgga cgctgcggtg acggacagca ctgctgcccc tgctgcaaag ccgaagaggg cggctgtagc tgcggctacg cctgcgcctg ccccgtctgg ggagacgagg ctcggcattg cggcgaggcg cgaggagaac ttctctgcgt ggtacatcga tgtgatcaca aaggcggaga tggtcgagta ctacgacgtg tccgggtgct acatcatccg tccgtgggcg tactacgtgt ggaagtgcgt gcagaggttc cttggaggga ggatcgagag gcttggcgtg gaggactgct actttccgat gttcgtgtcg cgcagctgcc tggagcgcga gaaggaccac atcgagggct ttgcgccgga ggttgcgtgg gtgacgcgtg ccggcgacac ggagttggag cagccggtcg ctgtgcggcc aacaagcgag acggtgatgt acccgtacta cgcgaagtgg atccggtcgc accgcgacct gcccgtgcga ctgaacatgt ggaacaacgt gattcggtgg gagttctcgc acccgacgcc gttcattcgg acgcgcgagt tcctgtggca ggagggacac tgcgcgtggg cgaaggcgga ggactgcgcg aaggaggtgc tggacatcct ggagtgctat gcggctgtgt acgagcagct gctggcggtg ccggtggtgc gcgggcgcaa gacggagaag gagaagttcg ctggtgggtc ctacacgacg acggtggaga cgttcatcga ggctgttggg cgcgggtgcc agggcgcgac gagccacaac ctggggcaga acttcggcaa gatgttcgac atccgcttcc aggacccgga gaacaatgag cagacgttga tcccgtggca gaacagctgg gggctgtcga cgcgcgtgat cggcgtgatg atcatggtgc acggcgacaa ccgcggcatg gtgatgccgc cgcgcgttgc gtcgacgcag gtgatcatcg tcccggtggg gatcacgaag gacacgacgg aggaggcgcg gcaggcgctg cttgcgagct gccggcagct ggagggcgag ctgtgcgagg gcggcgtgcg cgcgaagtgc gacctgcgcg acaactacag ccctgggtgg cggttcaacc actgggaggt gaagggcgtg ccgctgcgcg tggagcttgg gccgagggaa cttgcggagc ggtcgcttgt ggttgctgtg cggcacagct gcgcgaagca gtcgcttacg tggaacgcgc agacgccgac agcggtcgct gcgctgctgg aggacgtgca ggctcagatg tacgcgcggg cgaaggagac gatggagacg caccgcgtgc gtgtgacgga gtgggcggag tttgtgccgg cactgaaccg gaagtgcctg atccttgcgc cgtggtgcgg ggcgatagag tgcgaggacc aggtgcggaa agacagcgcg gaggagtcga gggctgcgca ggcgcaggag gtacgcgagg acgcgcgcgc gccgagcatg ggtgcgaaga cgctgtgcat cccattttcg cagcccgagc ccgcggagag ccacacgtgc atctgcaagg gctgcacgaa ggcggcgacg acgtgggtgc tgtttggccg cagctactga

Details for LebrA.18681.a.B1.GE42666

HARVESTED ON:	9/28/2018
SEQUENCED ON:	10/17/2018
EXPECTED MW:	82kDa
OBSERVED MW:	82kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS IEDCRGFDEV KALFQELGLD IPILHHDEKA TVEEVLDLLK EKMGITAVGT KTLFLKSKKG DLVMATALKS TATELKFIQN VTNTKDLRFA SNEVLHECLA VVQGCVTPFP LLNHTENRPI TLLLDTSMAH STQLLAFCLC RNDYTAAITF QQLKMYLEKI GYAYKLVDFG AASADAAVTD STAAPAAKPK RAAVAAATPA PAPSGETRLG IAARREENFS AWYIDVITKA EMVEYYDVSG CYIIRPWAYY VWKCVQRFLG GRIERLGVED CYFPMFVSRS CLEREKDHIE GFAPEVAWVT RAGDTELEQP VAVRPTSETV MYPYYAKWIR SHRDLPVRLN MWNNVIRWEF SHPTPFIRTR EFLWQEGHCA WAKAEDCAKE VLDILECYAA VYEQLLAVPV VRGRKTEKEK FAGGSYTTTV ETFIEAVGRG CQGATSHNLG QNFGKMFDIR FQDPENNEQT LIPWQNSWGL STRVIGVMIM VHGDNRGMVM PPRVASTQVI IVPVGITKDT TEEARQALLA SCRQLEGELC EGGVRAKCDL RDNYSPGWRF NHWEVKGVPL RVELGPRELA ERSLVVAVRH SCAKQSLTWN AQTPTAVAAL LEDVQAQMYA RAKETMETHR VRVTEWAEFV PALNRKCLIL APWCGAIECE DQVRKDSAEE SRAAQAQEVR EDARAPSMGA KTLCIPFSQP EPAESHTCIC KGCTKAATTW VLFGRSY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcca tcgaggactg ccgcggcttt gatgaggtga aggccctctt ccaagagcta gggcttgaca ttcccattct gcaccatgac gagaaagcca ccgtcgagga ggtgctggac cttctgaagg agaagatggg catcaccgcg gtgggcacca agacactctt cctcaagagc aaaaaaggcg acctggtgat ggcgacggcg ctcaagtcaa ccgcgacaga actcaagttc attcaaaacg ttactaacac caaggacctg cgctttgcct ccaacgaggt gttgcatgag tgcctcgccg tcgtgcaggg atgcgtcacg ccgttcccac tcctcaacca cacagaaaac cgtcccatta cgctcctact ggacacctca atggcgcaca gcacgcagct gctcgccttt tgtctgtgcc gtaacgatta caccgccgct atcacattcc agcagctcaa gatgtacctc gagaagatcg ggtacgcgta caaactggtg gactttggtg ctgcctcggc ggacgctgcg gtgacggaca gcactgctgc ccctgctgca aagccgaaga gggcggctgt agctgcggct acgcctgcgc ctgccccgtc tggggagacg aggctcggca ttgcggcgag gcgcgaggag aacttctctg cgtggtacat cgatgtgatc acaaaggcgg agatggtcga gtactacgac gtgtccgggt gctacatcat ccgtccgtgg gcgtactacg tgtggaagtg cgtgcagagg ttccttggag ggaggatcga gaggcttggc gtggaggact gctactttcc gatgttcgtg tcgcgcagct gcctggagcg cgagaaggac cacatcgagg gctttgcgcc ggaggttgcg tgggtgacgc gtgccggcga cacggagttg gagcagccgg tcgctgtgcg gccaacaagc gagacggtga tgtacccgta ctacgcgaag tggatccggt cgcaccgcga cctgcccgtg cgactgaaca tgtggaacaa cgtgattcgg tgggagttct cgcacccgac gccgttcatt cggacgcgcg agttcctgtg gcaggaggga cactgcgcgt gggcgaaggc ggaggactgc gcgaaggagg tgctggacat cctggagtgc tatgcggctg tgtacgagca gctgctggcg gtgccggtgg tgcgcgggcg caagacggag aaggagaagt tcgctggtgg gtcctacacg acgacggtgg agacgttcat cgaggctgtt gggcgcgggt gccagggcgc gacgagccac aacctggggc agaacttcgg caagatgttc gacatccgct tccaggaccc ggagaacaat gagcagacgt tgatcccgtg gcagaacagc tgggggctgt cgacgcgcgt gatcggcgtg atgatcatgg tgcacggcga caaccgcggc atggtgatgc cgccgcgcgt tgcgtcgacg caggtgatca tcgtcccggt ggggatcacg aaggacacga cggaggaggc gcggcaggcg ctgcttgcga gctgccggca gctggagggc gagctgtgcg agggcggcgt gcgcgcgaag tgcgacctgc gcgacaacta cagccctggg tggcggttca accactggga ggtgaagggc gtgccgctgc gcgtggagct tgggccgagg gaacttgcgg agcggtcgct tgtggttgct gtgcggcaca gctgcgcgaa gcagtcgctt acgtggaacg cgcagacgcc gacagcggtc gctgcgctgc tggaggacgt gcaggctcag atgtacgcgc gggcgaagga gacgatggag acgcaccgcg tgcgtgtgac ggagtgggcg gagtttgtgc cggcactgaa ccggaagtgc ctgatccttg cgccgtggtg cggggcgata gagtgcgagg accaggtgcg gaaagacagc gcggaggagt cgagggctgc gcaggcgcag gaggtacgcg aggacgcgcg cgcgccgagc atgggtgcga agacgctgtg catcccattt tcgcagcccg agcccgcgga gagccacacg tgcatctgca agggctgcac gaaggcggcg acgacgtggg tgctgtttgg ccgcagctac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat