LebrA.18664.a

kinetoplastid kinetochore protein 2, putative (kkt2)

CENTER ID: LebrA.18664.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.18664.a.B2.GE42788 PHoranyi kinase domain truncation 52 346
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.35.5600
UniProt: A4HQ49

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSHFRGSFSR TPPRSGAISM PRDLSQTPAI SRLGSTVKTP HIQKCVVDQT EDDDHPLEHM TVYFEEEDLR LVTTGLLGKG GFGKVFDAVS NSGDAYALKV SSKRMSENDW KRLKEEVTLM SHFSRHPNIV KFYAAGKDED RAYVVMERCA GKSLHDIIAS RSLDVPEILW IGWALISTIS YIHSKGCIHR DLKPQNLLFD TEGNLKITDF GLSSRISEAH PRKTVAGTAM YMAPEMAVEV YKRMTQNSDA PSLSYGKEVD TWSIGVVLYV LLTRMNPYLE AMEQKGMHQL NKGHKSLALF NAVAGAAWSW PREWKGDPQL CRLVERVLHR EASRRATLME VLEDSVWNRR PLSCPLSLLQ KLNLLEPSPS GALPLNNLAE NLQFRPKRTT EAVLREGLER VEATEQRGRA QVELEYYETY NVLWSLLTLA RAEVDTRADI LQSEVVQRGK LRNQFLTRQS TRDRGGSVSL VSEVADCETT TPRASRSVRR SVSLTEEERG RIVRSSPVQY AVVYPGRDTA TRWNLRAVVS LPRDMTDEIE REFKCMNGHL MTKLTSMPHG YNGFDCNVCD RGILKITGES PAFRCYKCDY DVCMKCAYSG KFKDVSFVCV TCAKRFTSTA KLQGHSLRCR GPSESPSPRR SSRMNTMLWD EPKRPSLLEV QLPEASQSES KLHASRCRSG RRTYNRTSTG GRISIGDPNV HSVVDFDAMV AEHREADFPD VSACASATGR ELSQQRKLAG NRRGRPSTMS SGSLSLDLPP QVQVPSKGSR APVQPRSSAE LRDIMEEVEQ QKQAMRRDPL LYGPATPPQY NSNGEIIGIA ARRRAESVEM TRAEVITIRA EVANRPHELQ RQPRVPRSAS SSRLENAPQS LHKRRREEWQ QPVHAPSPSG AVNRVAVEEH VAKHAVLPPQ VPRGKGQQPR APSVSGHTAQ GGPPLPRRGP AAPSPAATLK THLSAFQIPA AIPPKNFTSI RQSSYSMANT MAATYSASAT EPADRAGAAN AALGYGAAST RSHALSRPNG AFLALPREEH SRRQFLDDFL SGGWVRFYSF TNEDTVVMYY SLQPGRYGAM FPTEAGVGTA VLDVYAKLVL YVPCMNNEST NRSQPHPHVQ TFYDEEARIL SLTEAQRYLG GVLRCITGFV DEFSRLKSEG LTPAAVHAAY IHHRSMSHVP RDTKFVYIRK VFPDPSGSFT LFRLSNLRSQ VVCNAMVDIR WQSDRRHNVG QKYYINADGT AEPFLVDQTG ILSQLETVLN NSFRR
NT Sequence
atgtcgcact ttcgcggctc tttctcgcga actccaccaa ggagtggggc gatttctatg ccgcgtgact tgtcacagac ccctgccatc tctcgacttg gaagcactgt gaagacgcca cacatccaaa agtgtgtcgt tgaccaaaca gaggacgatg atcacccact agagcacatg acggtctact tcgaggagga ggaccttaga ctagttacca ctgggcttct cggaaagggt ggttttggaa aggtgttcga tgccgtttcc aacagcggtg atgcttacgc gctcaaggtg tcatcaaagc gcatgagcga aaatgactgg aagcgactaa aggaggaagt gacgctcatg agtcatttct cgcgccaccc caacattgtc aaattttacg ctgctggtaa ggatgaagat cgcgcctacg tggtaatgga gcgctgcgca ggcaagtcac tccacgatat catagccagc aggagtcttg atgtgccgga gattttgtgg attgggtggg ccctgatcag caccatctcc tacattcatt ccaaaggctg cattcaccgc gacctgaagc cacagaatct tttgtttgac actgagggta atttaaagat tacagatttt ggtctttcca gccgcatatc agaggcgcat ccccgcaaga cggttgcggg gacggcaatg tacatggcgc ctgaaatggc agttgaggtt tacaagcgaa tgacccagaa ctcggacgcc ccgtcactga gctacggcaa ggaggtggac acgtggagta tcggtgtggt cctctatgtg ctcttgacac gcatgaatcc atatctcgag gcgatggagc aaaaaggtat gcaccagctg aacaagggac acaagtcact tgccctcttt aatgctgtag cgggcgctgc gtggagttgg ccaagagagt ggaaaggtga tccacagctc tgcaggcttg tggaacgcgt gttgcaccgc gaggcatcga ggcgcgccac gctaatggag gtgctcgagg actctgtgtg gaaccgccgg cccctctcct gtccactttc tctgctacag aagctcaact tgctggagcc ttcgccgtca ggtgcactgc ctctgaacaa ccttgccgag aatctacaat tccgcccgaa gcgcacgaca gaggcggtgc tacgcgaagg actagagcgt gtcgaagcca ctgagcaacg cggtcgtgct caggtggagc ttgagtacta tgaaacctac aatgtcctct ggagtcttct tactctggcg cgggcggaag tggacaccag ggctgacatc ctccagtctg aggtggtgca gcgagggaag ctgcgcaatc agtttctgac tcgccagtct actcgtgaca ggggtggcag cgtctcgttg gtgtcagagg ttgccgattg cgagacaacc acccctcgtg catctcgatc agtgcgtcgc agcgtcagct tgaccgagga ggagcggggc agaatcgtgc gttctagccc ggtccagtac gcagtggtgt accccggacg cgacaccgcc actcgttgga acctccgtgc cgtagtctcg cttccacgcg acatgacgga cgaaatcgag cgcgagttca agtgtatgaa cgggcatctg atgacaaagt tgacgtcgat gccgcacggc tacaatggtt tcgactgcaa tgtgtgcgat cgtggcattc tcaagatcac cggcgagtcg ccagccttcc gatgctacaa gtgtgactac gatgtgtgca tgaagtgcgc atactccggc aagtttaaag acgtcagctt tgtctgcgtg acatgcgcga agcgcttcac ttcgaccgca aagctgcagg ggcattcctt gcgctgtcgc ggaccgagcg aaagtccctc accgcgacga tcgtcgcgca tgaacaccat gctctgggac gagccgaaga gaccgagctt actggaggtg cagctgcctg aagcctccca gagcgagtcg aagctacacg ccagccgctg ccgctcggga cgccgcacgt acaaccgcac atccaccggt ggccgcatca gcattgggga cccgaatgtg cacagtgtgg tggactttga cgcaatggtg gccgagcacc gcgaggcgga ctttcccgat gtgagtgcat gcgcgtccgc cactggccgc gaactctcgc agcaacgaaa gctcgcgggt aatcggcgcg gccggccatc caccatgtcc agcggcagcc tttcgctaga cttgccgccg caagtgcagg taccaagcaa ggggtcgcgc gcaccagtgc agccacgcag ctccgccgag ctgcgcgata tcatggagga ggtggaacaa cagaagcagg caatgcgccg tgaccccctc ttgtacggac cggctacgcc gccgcagtac aatagcaacg gcgagattat cggcatcgcg gctcgtcgcc gcgcagagag cgtggagatg acacgggcag aggtaatcac gattcgtgca gaggtcgcga atcggccgca cgagctgcag cgtcaaccgc gcgtaccacg cagcgcctcc tcatcacgtc tagagaatgc gcctcagagc ctccacaagc ggcgtcgcga ggagtggcag cagcccgtgc atgcaccatc gccatccggt gcggtgaacc gagtcgccgt ggaggagcac gtggcgaagc atgctgtcct gccgcctcag gtgccgcgcg ggaaaggaca acaaccacgc gccccctccg ttagtgggca taccgcccag ggtggcccgc cactaccgcg ccgcggccca gccgcgccgt ctccagcagc aactttgaag acccacctca gcgccttcca gatccccgct gccattcctc ctaagaactt tacctccatc cggcagtcga gctactccat ggcgaacacg atggcagcca cgtacagcgc ttccgcaacc gagccggcag accgagcagg agctgcgaac gctgctcttg gctacggggc tgcttcgaca cgcagccatg cgctaagccg acccaacggt gcctttttgg cgttgccgcg cgaggagcac agtcgccggc aattcttgga cgactttctt agtggtggct gggtgcgttt ctactccttc acaaacgagg acactgtcgt catgtactac tcactgcaac ctggtcgcta cggggccatg ttccccaccg aggcgggcgt cggcactgct gtgttagatg tgtacgcgaa actggtcctc tacgtaccgt gcatgaacaa tgagagcacg aaccgcagtc aaccccaccc acacgtgcaa acgttctacg acgaggaggc gcgtattctt agcctgactg aggcgcaacg gtacctaggc ggtgtgctac gctgcatcac aggatttgta gatgagttta gtcgcttgaa atccgagggt cttactccag cggcggtgca tgctgcctat attcatcacc gtagcatgtc ccatgtgccg cgggacacga agtttgtgta cattcgcaaa gtgttccccg acccctctgg gtctttcaca ctattccgcc tgtcaaacct gcgctcgcaa gtcgtttgta acgccatggt agatattcga tggcagagtg atcggcgcca caacgttggg caaaaatatt acatcaacgc ggacggtacc gctgagcctt ttctcgtcga tcaaaccggc attttgtcgc agctggagac ggtcctcaat aacagcttca ggaggtga
Details for LebrA.18664.a.B2.GE42788
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD DDHPLEHMTV YFEEEDLRLV TTGLLGKGGF GKVFDAVSNS GDAYALKVSS KRMSENDWKR LKEEVTLMSH FSRHPNIVKF YAAGKDEDRA YVVMERCAGK SLHDIIASRS LDVPEILWIG WALISTISYI HSKGCIHRDL KPQNLLFDNE GNLKITDFGL SSRISEAHPR KTVAGTAMYM APEMAVEVYK RMTQNSDAPS LSYGKEVDTW SIGVVLYVLL TRMNPYLEAM EQKGMHQLNK GHKSLALFNA VAGAAWSWPR EWKGDPQLCR LVERVLHREA SRRATLXXVL EDSV
Validated NT Sequence
ttactcactt acacagagtc ctcgagcacn nnnnntagcg tggcgcgcct cgatgcctcg cggtgcaaca cgcgttccac aagcctgcag agctgtggat cacctttcca ctctcttggc caactccacg cagcgcccgc tacagcatta aagagggcaa gtgacttgtg tcccttgttc agctggtgca tacctttttg ctccatcgcc tcgagatatg gattcatgcg tgtcaagagc acatagagga ccacaccgat actccacgtg tccacctcct tgccgtagct cagtgacggg gcgtccgagt tctgggtcat tcgcttgtaa acctcaactg ccatttcagg cgccatgtac attgccgtcc ccgcaaccgt cttgcgggga tgcgcctctg atatgcggct ggaaagacca aaatctgtaa tctttaaatt accctcattg tcaaacaaaa gattctgtgg cttcaggtcg cggtgaatac agcctttgga atgaatgtag gagatggtgc tgatcagggc ccacccaatc cacaaaatct ccggcacatc aagactcctg ctggctatga tatcgtggag tgacttgcct gcgcagcgct ccattaccac gtaggcgcga tcttcatcct taccagcagc gtaaaatttg acaatgttgg ggtggcgcga gaaatgactc atgagtgtca cttcctcctt tagtcgcttc cagtcatttt cgctcatgcg ctttgatgac accttgagcg cgtaagcatc accgctgttg gaaacggcat cgaacacctt tccaaaacca ccctttccga gaagcccagt ggtaactagt ctaaggtcct cctcctcgaa gtagaccgtc atgtgctcca gtgggtgatc atcgtccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHDD DHPLEHMTVY FEEEDLRLVT TGLLGKGGFG KVFDAVSNSG DAYALKVSSK RMSENDWKRL KEEVTLMSHF SRHPNIVKFY AAGKDEDRAY VVMERCAGKS LHDIIASRSL DVPEILWIGW ALISTISYIH SKGCIHRDLK PQNLLFDTEG NLKITDFGLS SRISEAHPRK TVAGTAMYMA PEMAVEVYKR MTQNSDAPSL SYGKEVDTWS IGVVLYVLLT RMNPYLEAME QKGMHQLNKG HKSLALFNAV AGAAWSWPRE WKGDPQLCRL VERVLHREAS RRATLMEVLE DSV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgacgatg atcacccact agagcacatg acggtctact tcgaggagga ggaccttaga ctagttacca ctgggcttct cggaaagggt ggttttggaa aggtgttcga tgccgtttcc aacagcggtg atgcttacgc gctcaaggtg tcatcaaagc gcatgagcga aaatgactgg aagcgactaa aggaggaagt gacgctcatg agtcatttct cgcgccaccc caacattgtc aaattttacg ctgctggtaa ggatgaagat cgcgcctacg tggtaatgga gcgctgcgca ggcaagtcac tccacgatat catagccagc aggagtcttg atgtgccgga gattttgtgg attgggtggg ccctgatcag caccatctcc tacattcatt ccaaaggctg cattcaccgc gacctgaagc cacagaatct tttgtttgac actgagggta atttaaagat tacagatttt ggtctttcca gccgcatatc agaggcgcat ccccgcaaga cggttgcggg gacggcaatg tacatggcgc ctgaaatggc agttgaggtt tacaagcgaa tgacccagaa ctcggacgcc ccgtcactga gctacggcaa ggaggtggac acgtggagta tcggtgtggt cctctatgtg ctcttgacac gcatgaatcc atatctcgag gcgatggagc aaaaaggtat gcaccagctg aacaagggac acaagtcact tgccctcttt aatgctgtag cgggcgctgc gtggagttgg ccaagagagt ggaaaggtga tccacagctc tgcaggcttg tggaacgcgt gttgcaccgc gaggcatcga ggcgcgccac gctaatggag gtgctcgagg actctgtgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat