LebrA.18331.a

Putative fumarate hydratase (EC 4.2.1.2) (class I)

CENTER ID: LebrA.18331.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.18331.a.B1.GE42065 full length 1 549
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.24.0320
UniProt: A4HD88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFRRITPLLS EFNFVPLVSK VSHKETKYRL LTKDHVSVVQ PGAGLPEMLK VDPAALTLLS SVAFDDIEHL MRSSHLASLR KIFDDPEASD NDKFVALQLL KNANISSARL LPGCQDTGTA IISGHRGEQV FVLGNEEEAL SRGVYDIFQE RNLRYSQNVP LSMYDEKNTG TNLPAQIDLY ATKGMEYSFL FVAKGGGSAN KSFLLQESKS VLNPKSLRKF LKEKLAMFGT SACPPYHIAV VIGGTSAEMT MRMVKHASCH YYDDLITKPD MKTGYTFRDL ELEKEVLEIC QNIGMGAQFG GKYYAHDARV IRMPRHGASC PIGIGVSCSA DRQALGKINK DGVWLEVLEM EPSRFLPDVK EDELLKTPPV EVNLNRPMSE VLQELSKHPV KTRLSLTGTI VVARDSAHAR MREMLEAGKP LPQYMKDHPV YYAGPAKTPD GLPSGSFGPT TAGRMDPFVD LFQSLGGSMV MLAKGNRSKH VTEACKKHGG FYLGSIGGPA AVLAQDAIKK VECLDMKDLG MEAVWKIEVQ NFPAFIVVDD KGNDFFQQL
NT Sequence
atgtttcgcc gcattacccc gctactgtca gagttcaact ttgtgccact ggtgtccaag gtgtcgcaca aggagaccaa ataccgtctc ttgacgaagg accatgtctc tgtggtgcag cccggtgccg gactgccgga gatgctgaag gtggacccgg ctgcactcac gttgctgtcc tccgtggcgt tcgacgatat agagcacctg atgcgctcct cgcatctggc gtcactgcgc aaaatcttcg atgaccccga agcgagcgac aacgacaagt tcgtagcgct gcagctactt aagaatgcaa acatctcctc ggcccgtttg ctgcccggct gccaggacac cggaacggcc atcatctcgg gccaccgtgg cgagcaggtg tttgtgctcg gcaacgagga ggaggccctc agccgtggtg tgtacgatat cttccaggag cgcaacctcc gctacagcca gaacgtaccg ctcagcatgt atgacgagaa gaacaccggc acgaacttgc cagcccagat tgatctgtac gccaccaagg ggatggagta cagcttcttg tttgttgcga aggggggcgg ctcggcaaac aaatcgttcc tgctgcaaga gagcaagtcg gtgctcaacc ccaagtcgct gcgtaagttc ctcaaggaga agctagccat gtttggcacc tctgcctgcc cgccgtacca catcgccgtc gtcattggtg gcacgagcgc tgagatgacg atgaggatgg tgaaacacgc ctcctgccac tactacgacg acctgatcac gaagccagac atgaaaacag gctacacgtt ccgcgacctc gagctggaga aggaggtact ggagatctgc cagaacatcg ggatgggtgc ccagtttggc ggcaagtact acgcccatga cgcgcgcgtg atccgcatgc ctcgccatgg tgcgtcctgc cctatcggca taggtgtctc ttgcagcgcg gatcgccagg cgctcggcaa gattaacaaa gatggcgtgt ggttggaggt actggagatg gagccgtcca ggttcctgcc ggacgtgaag gaggatgagc tgcttaaaac gccgccggtc gaggtgaatc tgaaccgacc catgtcagag gtccttcagg agctctccaa gcaccccgtg aaaacccgcc tcagcctcac aggcaccatc gttgtggcgc gcgacagcgc ccatgcccga atgcgtgaga tgctagaggc tggcaagccg ctgccacagt acatgaaaga tcaccccgtg tactacgccg gcccagcgaa gacgcccgac ggtcttccgt ccggatcctt cggcccaacg acggctggtc gcatggaccc cttcgtcgac ctctttcagt cgctgggcgg ctcgatggtg atgctcgcca agggcaaccg cagcaagcac gtgacggagg catgcaaaaa gcacggcggc ttctacctcg gcagcatcgg cgggccggcg gctgtgttag cgcaggatgc gatcaaaaaa gtggagtgcc tcgacatgaa ggacctcggg atggaggcgg tgtggaagat cgaagtgcag aacttcccgg ccttcatcgt tgtcgatgat aagggcaacg acttcttcca gcagctctag
Details for LebrA.18331.a.B1.GE42065
HARVESTED ON: data unavailable
SEQUENCED ON: 12/7/2017
EXPECTED MW: 62kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 97
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMF RRITPLLSEF NFVPLVSKVS HKETKYRLLT KDHVSVVQPG AGLPEMLKVN PAALTLLSSV AFDDIEHLMR SSHLASLRKI FDDPEASDND KFVALQLLKN ANISSARLLP GCQDTGTAII SGHRGEQVFV LGNEEEALSR GVYEIFQERN LRYSQNVPLS MYDEKNTGTN LPAQIDLYAT KGMEYSFLFV AKGGGSANKS FLLQXSKSVL NPKSLRKFLK EKLAXFGTSA CPPYHIAXXI GGTSAEMTMR MVKHAXCHYX DDLITKPDMK TGYTFRDLEL EKEVLEICXN IGMGAQFGGK YYAHDARVIR MPRHGASCPI GIGVSCSADR QALGKINKDG VWLEVLEMEP SRFLPDVKED ELLKTPPVEV NLNRPMSEVL QELSKHPVKT RLSLTGTIVV ARDSAHARLR EMLEAGKPLP QYMKDHPVYY AGPAKTPDGL PSGSFGPTTA GRMDPFVDLF QSLGGSMVML AKGNRSRHVT EACKKHGGFY LGSIGGPAAV LAQDAIKKVE CLDMKDLGME AVWKIEVQNF PAFIVVDDK
Validated NT Sequence
gcccttatca tcgacaacga tgaaggccgg gaagttctgc acttcgatct tccacaccgc ctccatcccg aggtccttca tgtcgaggca ctccactttt ttgattgcat cctgcgctag cacagccgct ggcccgccaa tgctgccgag gtagaagccg ccgtgctttt tgcatgcctc cgtcacgtgc ctgctgcggt tgcccttggc gagcatcacc atcgagccgc ccagcgactg gaagaggtcg acgaaggggt ccatgcgacc agccgtcgtt gggccgaagg atccggacgg aaggccgtcg ggcgtcttcg ctgggccggc gtagtacacg gggtgatctt tcatgtactg tggcagcggc ttgccagcct ccagcatctc acgcaaccgg gcatgggcac tgtcgcgcgc cacaacgatg gtgcctgtga ggctgaggcg ggttttcacg gggtgcttgg agagctcctg aaggacctcg gacatgggtc ggttcagatt cacctcgacc ggcggcgttt tgagcagctc atcctctttc acgtccggca ggaacctgga cggctccatc tccagtacct ccaaccacac gccatccttg ttaatcttgc caagcgcctg gcgatccgcg ctgcaagaga cacctatgcc gatagggcag gacgcaccat ggcgaggcat gcggatcacg cgcgcgtcat gggcatagta cttgccgcca aactgggcac ccatcccgat gttcnngcag atctccagca cctccttctc cagctcgagg tcgcggaacg tgtagcctgt tttcatgtct ggcttcgtga tcaggtcgtc ntagtagtgg cannnggcgt gtttcaccat cctcatcgtc atctcagcgc tcgtgccacc aattnnnnng gcgatgtggt acggcgggca ggcagaggtg ccaaacanng ctagcttctc cttgaggaac ttacgcagcg acttggggtt gagcaccgac ttgctctntt gcagcaggaa cgatttgttt gccgagccgc cccccttcgc aacaaacaag aagctgtact ccatcccctt ggtggcgtac agatcaatct gggctggcaa gttcgtgccg gtgttcttct cgtcatacat gctgagcggt acgttctgac tgtagcggag gttgcgctcc tggaagatct cgtacacgcc gcggctgagg gcctcctcct cgttgccgag cacaaacacc tgctcgccac ggtggcccga gatgatggcc gttccggtgt cctggcagcc gggcagcaaa cgggccgagg agatgtttgc attcttaagc agctgcagcg ctacgaactt gtcgttatcg ctcgcttcgg gatcatcgaa gattttgcgc agtgacgcca gatgcgagga gcgcatcagg tgctctatat cgtcgaacgc cacggaggac agcaacgtga gtgcagcagg gttcaccttc agcatctccg gcagtccggc accgggctgc accacagaga catggtcctt cgtcaagaga cggtatttgg tctccttgtg cgacaccttg gacaccagtg gcacaaagtt gaactctgac agtagcgggg taatgcggcg aaacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMF RRITPLLSEF NFVPLVSKVS HKETKYRLLT KDHVSVVQPG AGLPEMLKVD PAALTLLSSV AFDDIEHLMR SSHLASLRKI FDDPEASDND KFVALQLLKN ANISSARLLP GCQDTGTAII SGHRGEQVFV LGNEEEALSR GVYDIFQERN LRYSQNVPLS MYDEKNTGTN LPAQIDLYAT KGMEYSFLFV AKGGGSANKS FLLQESKSVL NPKSLRKFLK EKLAMFGTSA CPPYHIAVVI GGTSAEMTMR MVKHASCHYY DDLITKPDMK TGYTFRDLEL EKEVLEICQN IGMGAQFGGK YYAHDARVIR MPRHGASCPI GIGVSCSADR QALGKINKDG VWLEVLEMEP SRFLPDVKED ELLKTPPVEV NLNRPMSEVL QELSKHPVKT RLSLTGTIVV ARDSAHARMR EMLEAGKPLP QYMKDHPVYY AGPAKTPDGL PSGSFGPTTA GRMDPFVDLF QSLGGSMVML AKGNRSKHVT EACKKHGGFY LGSIGGPAAV LAQDAIKKVE CLDMKDLGME AVWKIEVQNF PAFIVVDDKG NDFFQQL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtttc gccgcattac cccgctactg tcagagttca actttgtgcc actggtgtcc aaggtgtcgc acaaggagac caaataccgt ctcttgacga aggaccatgt ctctgtggtg cagcccggtg ccggactgcc ggagatgctg aaggtggacc cggctgcact cacgttgctg tcctccgtgg cgttcgacga tatagagcac ctgatgcgct cctcgcatct ggcgtcactg cgcaaaatct tcgatgaccc cgaagcgagc gacaacgaca agttcgtagc gctgcagcta cttaagaatg caaacatctc ctcggcccgt ttgctgcccg gctgccagga caccggaacg gccatcatct cgggccaccg tggcgagcag gtgtttgtgc tcggcaacga ggaggaggcc ctcagccgtg gtgtgtacga tatcttccag gagcgcaacc tccgctacag ccagaacgta ccgctcagca tgtatgacga gaagaacacc ggcacgaact tgccagccca gattgatctg tacgccacca aggggatgga gtacagcttc ttgtttgttg cgaagggggg cggctcggca aacaaatcgt tcctgctgca agagagcaag tcggtgctca accccaagtc gctgcgtaag ttcctcaagg agaagctagc catgtttggc acctctgcct gcccgccgta ccacatcgcc gtcgtcattg gtggcacgag cgctgagatg acgatgagga tggtgaaaca cgcctcctgc cactactacg acgacctgat cacgaagcca gacatgaaaa caggctacac gttccgcgac ctcgagctgg agaaggaggt actggagatc tgccagaaca tcgggatggg tgcccagttt ggcggcaagt actacgccca tgacgcgcgc gtgatccgca tgcctcgcca tggtgcgtcc tgccctatcg gcataggtgt ctcttgcagc gcggatcgcc aggcgctcgg caagattaac aaagatggcg tgtggttgga ggtactggag atggagccgt ccaggttcct gccggacgtg aaggaggatg agctgcttaa aacgccgccg gtcgaggtga atctgaaccg acccatgtca gaggtccttc aggagctctc caagcacccc gtgaaaaccc gcctcagcct cacaggcacc atcgttgtgg cgcgcgacag cgcccatgcc cgaatgcgtg agatgctaga ggctggcaag ccgctgccac agtacatgaa agatcacccc gtgtactacg ccggcccagc gaagacgccc gacggtcttc cgtccggatc cttcggccca acgacggctg gtcgcatgga ccccttcgtc gacctctttc agtcgctggg cggctcgatg gtgatgctcg ccaagggcaa ccgcagcaag cacgtgacgg aggcatgcaa aaagcacggc ggcttctacc tcggcagcat cggcgggccg gcggctgtgt tagcgcagga tgcgatcaaa aaagtggagt gcctcgacat gaaggacctc gggatggagg cggtgtggaa gatcgaagtg cagaacttcc cggccttcat cgttgtcgat gataagggca acgacttctt ccagcagctc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat