LebrA.18247.a

Putative mitogen-activated protein kinase kinase 3

CENTER ID: LebrA.18247.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.18247.a.HZ2.GE43705 kinase domain 22 345
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.17.0070
UniProt: A4H8V2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYAVKRIDKR QAGTKGLRSV MGEVETMSLL SHPNIVKLEE TYQDDACLSI VMEYLPGGSL QHHIDRQSVS EGETRRIITQ LLMAVEYIHE KGIVHRDLKP SNCLLSQNDL VVKIADFGLS VFAGNEQCLT TCCGTLHFMA PEILLEKNYG KPVDMWAMGV MTHVMFLGRY PFQARTETAL TKDICRGYRL PEEGGDRPRC PPQLQDFISQ LLYYDPHRRM SAKDALKHRW IKEGLDANRR TSLYASQAPS AAVVKPSARW RAAVLTVMGA QRLLYLQKIK RLVRLGYDGF PILRDYRYLV TGNYVPASTS LECSHMFHAR PMALLELISM IDTCPLLKHV DLSWNNIHSL SIIQSLLKVV TRLPSLQSID LSHNPIPAVA GRSIVRLIRN PCSHVTNINI GDTGISADTI GQINSFLKEK LTHAALPYSI STGEMQSRGI QGGYDEGHMT SSSTTVTSLR QKANICSGPT KQPAKRDSHP VRLPPISRLP MARRNEKMS
NT Sequence
atgtacgcgg tgaagcgcat cgacaagcgt caggcaggca caaagggatt gcggagcgtt atgggggaag tggagacaat gagcctcctt tcccatccca acatcgtcaa gctcgaggag acgtatcaag acgacgcatg cctttctatt gtgatggaat acctccctgg tggttctctt cagcaccaca tagaccgaca aagcgtctct gaaggcgaga cgcggcgcat catcacgcag ttactgatgg ctgtagagta tattcatgaa aaggggatcg tacaccgcga tttgaagcca agcaactgcc tgctatcgca aaacgacctt gtcgtgaaga tagcagactt tggactctcc gtgtttgccg gtaatgagca atgtttgacg acgtgctgtg gcacgctgca cttcatggca cccgagatcc tgctagagaa gaactatgga aagcccgtgg acatgtgggc catgggtgtc atgacacacg tgatgtttct cggtcgttac ccgtttcagg cccgtacaga gactgcgctg acgaaagaca tctgccgtgg gtatcgtttg ccggaggagg gcggtgatag gccacgttgt cctccacagc ttcaagattt tatcagccaa cttctctatt acgacccaca ccgtcgaatg agcgccaaag atgctctcaa gcaccgatgg atcaaggagg gtttggatgc aaatcgacgg acgtccctct acgcatcaca agcaccttcg gcagcggtgg ttaagccatc cgcgcggtgg cgggcggcgg tgctcacagt catgggcgcg cagcgtctgt tgtacctgca gaaaattaag cggttagtgc ggcttggtta tgacgggttc cccattttgc gggactatcg ctatctcgtg accggcaatt atgtacctgc aagcacgtct ctagaatgta gtcacatgtt ccacgcacgg ccgatggcgc tgctcgagtt gatctcgatg atagacacgt gcccgctcct gaaacatgtc gatcttagct ggaataacat tcacagctta tctataattc agtcgctgct caaggtggtg acgcgactcc cttcactgca gtcgatcgat ttgtctcaca atccaatccc ggctgtggcc gggcgttcca ttgtgcgact gatccgcaac ccgtgttcgc acgtgacaaa catcaacatt ggggacaccg gaatcagcgc cgacaccatc ggacagatca actccttctt aaaggagaag ctgacccacg ccgctctgcc gtacagcatt tccacaggag agatgcaaag ccgaggcatc caaggtggct atgacgaggg acacatgaca tcgtcgtcaa ctacagtgac ctccttgagg caaaaggcta acatttgtag cgggcccacc aaacagccgg cgaagaggga ctcgcatccc gtgcggctgc ctccaatctc acgtctacca atggctaggc gcaatgagaa gatgtcgtag
Details for LebrA.18247.a.HZ2.GE43705
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FXKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMG EVETMSLLSH PNIVKLEETY QDDACLSIVM EYLPGGSLQH HIDRQSVSEG ETRRIITQLL MAVEYIHEKG IVHRDLKPSN CLLSQNDLVV KIADFGLSVF AGNEQCLTTC CGTLHFMAPE ILLEKNYGKP VDMWAMGVMA HVMFLGRYPF QARTETALTK DICRGYRLPE EGGNRPRCPP QLQDFISQLL YYDPHRRMSA KDALKHRWIK EGLDANRRTS LYASQAPSAA VVKPSARWRA AVLTVMGAQR LLYLQKIKRL VRLGYDGFPI LRDYRYLVTG NYVPASTSLE CSHMFHARPM ALLELISMID TCPLLKHVDL SW
Validated NT Sequence
attccagcta agatcgacat gtttcaggag cgggcacgtg tctatcatcg agatcaactc gagcagcgcc attggccgtg cgtggaacat gtgactacat tctagagacg tgcttgcagg tacataattg ccggtcacga gatagcgata gtcccgcaaa atggggaacc cgtcataacc aagccgcact aaccgcttaa ttttctgcag gtacaacaga cgctgcgcgc ccatgactgt gagcaccgcc gcccgccacc gcgcggatgg cttaaccacc gctgccgaag gtgcctgtga tgcgtagagg gacgtccgtc gatttgcatc caaaccctcc ttgatccatc ggtgcttgag agcatctttg gcgctcattc gacggtgtgg gtcgtaatag agaagttgac tgataaaatc ttgaagctgt ggaggacaac gtggcctatt accgccctcc tccggcaaac gatacccacg gcagatgtct ttcgtcagcg cagtctctgt acgggcctga aaggggtaac gaccgagaaa catcacgtgt gccatgacac ccatggccca catgtccacc ggttttccat agttcttctc tagcaggatc tcgggtgcca tgaagtgcag cgtgccacag cacgtcgtca aacattgttc attaccggca aacacggaga gtccaaagtc tgctatcttc acgacaaggt cgttttgcga tagcaggcag ttgcttggct tcaaatcgcg gtgtacgatc cccttttcat gaatatactc tacagccatc agtaactgcg tgatgatgcg ccgcgtctcg ccttcagaga cgctttgtcg gtctatgtgg tgttgaagag aaccaccagg gaggtattcc atcacaatag aaaggcatgc gtcgtcttga tacgtctcct cgagcttgac gatgttggga tgggaaagga ggctcattgt ctccacttcc cccatcgaac caggaccctg ggtctgagct tccagggtac caccaatctg ttctctgtga gcctcaataa tatcgttatc ctccatgtcc aaatcttcag gggtctgatc agcttgaatt ctaataccgt cgtacaagaa tcttaaggag tccatttcct taccctgtct tttagngaac gcttccatca gccttcttaa aggagtggtc tttttgatct tgaagaagat ctctgaagat ccatcggaca cctttaaatt gatgtgagtc tcaggcttga cttctggctt gacctctggc ttagcttctt gattgacttc tgagtccgac ttcatatgat ggtgatggtg atgagccat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSGE VETMSLLSHP NIVKLEETYQ DDACLSIVME YLPGGSLQHH IDRQSVSEGE TRRIITQLLM AVEYIHEKGI VHRDLKPSNC LLSQNDLVVK IADFGLSVFA GNEQCLTTCC GTLHFMAPEI LLEKNYGKPV DMWAMGVMTH VMFLGRYPFQ ARTETALTKD ICRGYRLPEE GGDRPRCPPQ LQDFISQLLY YDPHRRMSAK DALKHRWIKE GLDANRRTSL YASQAPSAAV VKPSARWRAA VLTVMGAQRL LYLQKIKRLV RLGYDGFPIL RDYRYLVTGN YVPASTSLEC SHMFHARPMA LLELISMIDT CPLLKHVDLS WN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgg gggaagtgga gacaatgagc ctcctttccc atcccaacat cgtcaagctc gaggagacgt atcaagacga cgcatgcctt tctattgtga tggaatacct ccctggtggt tctcttcagc accacataga ccgacaaagc gtctctgaag gcgagacgcg gcgcatcatc acgcagttac tgatggctgt agagtatatt catgaaaagg ggatcgtaca ccgcgatttg aagccaagca actgcctgct atcgcaaaac gaccttgtcg tgaagatagc agactttgga ctctccgtgt ttgccggtaa tgagcaatgt ttgacgacgt gctgtggcac gctgcacttc atggcacccg agatcctgct agagaagaac tatggaaagc ccgtggacat gtgggccatg ggtgtcatga cacacgtgat gtttctcggt cgttacccgt ttcaggcccg tacagagact gcgctgacga aagacatctg ccgtgggtat cgtttgccgg aggagggcgg tgataggcca cgttgtcctc cacagcttca agattttatc agccaacttc tctattacga cccacaccgt cgaatgagcg ccaaagatgc tctcaagcac cgatggatca aggagggttt ggatgcaaat cgacggacgt ccctctacgc atcacaagca ccttcggcag cggtggttaa gccatccgcg cggtggcggg cggcggtgct cacagtcatg ggcgcgcagc gtctgttgta cctgcagaaa attaagcggt tagtgcggct tggttatgac gggttcccca ttttgcggga ctatcgctat ctcgtgaccg gcaattatgt acctgcaagc acgtctctag aatgtagtca catgttccac gcacggccga tggcgctgct cgagttgatc tcgatgatag acacgtgccc gctcctgaaa catgtcgatc ttagctggaa ttaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa