LebrA.18236.a

KREL2

CENTER ID: LebrA.18236.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.18236.a.B1.GE40856 full length 1 418
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: LbrM.20.5890
RefSeq: XP_003723137.1
UniProt: E9AIM1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRRCLLAQL VRRSPILFSG EREALFERYS EIENAHSRRI EALKNAGLFN DEWIATEKVH GANFGIYSTE HGKTLQYAKR SGIMPPHEHF FGYHILIPDL ARYAQKGREL LMEHLGSSPS TVIINGELFG GKYDHPSVPK RRQSVMVAGR PRTITAVQNN AFPQYCPDLH FYAFDIKYRM TEEEDYCTMT YDEALAIFEK IPGLLYAKAI IRGPLNKVTA FDVEHFVTTI PPLVGMGDYP LKGNWSEGLV VKHRRRGEPG FDPKGLTIMK LKCTAFQEIS TDRRQGPRVD EMEEVRRQSI QVSGVQLPDM ESVIREPEVR QATQHLLSHV CENRLQSVLS KIGTDPFETQ SMTPNELSTL LAKDALKDFL KEVDAKIINA PLLIRREMTR YVLFEARKYV ASRWKDILGQ QKNADGES
NT Sequence
atgcttagac gatgcctgct tgcacagctc gtgcgccgct cgccgattct cttttcgggg gaaagggaag cgctgtttga gcgctatagt gagattgaaa acgcccactc acgacgcatt gaggcactga agaacgctgg tctcttcaat gatgagtgga ttgccacgga aaaggtccat ggcgctaact ttggcatcta ctcaacagaa cacggaaaga ctcttcagta cgcaaagcgg agcggcatca tgccgccgca cgagcatttc tttggctacc acatactgat accagatctc gcaaggtatg cccagaaagg ccgagaactg ctcatggaac atctcggctc ttcacccagc accgttatta ttaacgggga gctctttggc ggcaagtatg accacccaag cgtgccaaag cggcggcaga gcgtgatggt ggccggcagg ccgcgcacca ttacagctgt gcagaacaac gccttcccac agtactgccc agacctgcac ttctacgcct tcgacatcaa gtaccggatg accgaggaag aggattactg cactatgaca tacgacgaag ccctggccat ctttgagaaa attccgggcc tgctctacgc gaaggctatc attcgagggc cactgaacaa agtgaccgcg tttgacgtgg agcacttcgt cacgacaatt cctccgctgg tggggatggg cgactacccg ctcaagggaa actggtcgga gggcctcgtg gtaaagcacc gccgtcgtgg cgagcccggt ttcgacccaa aggggctcac catcatgaag ttgaagtgca ctgccttcca ggagatctcg accgatcgtc gccaggggcc gcgtgtagac gagatggagg aggttcggcg gcagtccatc caagtctccg gcgttcagct accggacatg gaaagcgtca ttcgagagcc cgaggtgcgc caggcgacac agcacctact tagccacgtg tgtgaaaatc gccttcagag tgtcctgtcc aaaatcggta ccgacccgtt cgagacgcag tctatgactc ccaacgagct ctccacgctg ctcgcaaaag atgcgctgaa ggacttcttg aaggaggtgg acgcaaagat catcaacgca ccgctactca tccgccgcga gatgacccgc tacgttctgt tcgaggcgcg caagtacgtt gccagtagat ggaaggacat cctcgggcag caaaagaatg cagacggtga gtcatga
Details for LebrA.18236.a.B1.GE40856
HARVESTED ON: data unavailable
SEQUENCED ON: 12/6/2016
EXPECTED MW: 49kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 65
Validated AA Sequence
MAHHHHHHML RRCLLAQLVR RSPILFSGER EALFERYSEI ENAHSRRIEA LKNAGLFNDE WIATEKVHGA NFGIYSTEHG KTLQYAKRSG IMPPHEHFFG YHILIPDLAR YAQKGRELLM ERLGSSPSTV IINGELFGGK YDHPSVPKRR QSVMVAGRPR TITAVQNNAF PQYCPDLHFY AFDIKYRMTE EEDYYTMTYD EALAIFEKIP GLLYAKAIIR GPLSKVTAFD VEHFVTTIPP LVGMGDYPLK GNWSEGLVVK HRRRGEPGFD P
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML RRCLLAQLVR RSPILFSGER EALFERYSEI ENAHSRRIEA LKNAGLFNDE WIATEKVHGA NFGIYSTEHG KTLQYAKRSG IMPPHEHFFG YHILIPDLAR YAQKGRELLM EHLGSSPSTV IINGELFGGK YDHPSVPKRR QSVMVAGRPR TITAVQNNAF PQYCPDLHFY AFDIKYRMTE EEDYCTMTYD EALAIFEKIP GLLYAKAIIR GPLNKVTAFD VEHFVTTIPP LVGMGDYPLK GNWSEGLVVK HRRRGEPGFD PKGLTIMKLK CTAFQEISTD RRQGPRVDEM EEVRRQSIQV SGVQLPDMES VIREPEVRQA TQHLLSHVCE NRLQSVLSKI GTDPFETQSM TPNELSTLLA KDALKDFLKE VDAKIINAPL LIRREMTRYV LFEARKYVAS RWKDILGQQK NADGES
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctta gacgatgcct gcttgcacag ctcgtgcgcc gctcgccgat tctcttttcg ggggaaaggg aagcgctgtt tgagcgctat agtgagattg aaaacgccca ctcacgacgc attgaggcac tgaagaacgc tggtctcttc aatgatgagt ggattgccac ggaaaaggtc catggcgcta actttggcat ctactcaaca gaacacggaa agactcttca gtacgcaaag cggagcggca tcatgccgcc gcacgagcat ttctttggct accacatact gataccagat ctcgcaaggt atgcccagaa aggccgagaa ctgctcatgg aacatctcgg ctcttcaccc agcaccgtta ttattaacgg ggagctcttt ggcggcaagt atgaccaccc aagcgtgcca aagcggcggc agagcgtgat ggtggccggc aggccgcgca ccattacagc tgtgcagaac aacgccttcc cacagtactg cccagacctg cacttctacg ccttcgacat caagtaccgg atgaccgagg aagaggatta ctgcactatg acatacgacg aagccctggc catctttgag aaaattccgg gcctgctcta cgcgaaggct atcattcgag ggccactgaa caaagtgacc gcgtttgacg tggagcactt cgtcacgaca attcctccgc tggtggggat gggcgactac ccgctcaagg gaaactggtc ggagggcctc gtggtaaagc accgccgtcg tggcgagccc ggtttcgacc caaaggggct caccatcatg aagttgaagt gcactgcctt ccaggagatc tcgaccgatc gtcgccaggg gccgcgtgta gacgagatgg aggaggttcg gcggcagtcc atccaagtct ccggcgttca gctaccggac atggaaagcg tcattcgaga gcccgaggtg cgccaggcga cacagcacct acttagccac gtgtgtgaaa atcgccttca gagtgtcctg tccaaaatcg gtaccgaccc gttcgagacg cagtctatga ctcccaacga gctctccacg ctgctcgcaa aagatgcgct gaaggacttc ttgaaggagg tggacgcaaa gatcatcaac gcaccgctac tcatccgccg cgagatgacc cgctacgttc tgttcgaggc gcgcaagtac gttgccagta gatggaagga catcctcggg cagcaaaaga atgcagacgg tgagtcatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at