LebrA.10201.a

methionyl-tRNA synthetase, putative

CENTER ID: LebrA.10201.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.10201.a.B2.GE42662 soluble domain 38 752
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.21.0900
UniProt: A4HBS8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMLRFFTDKA NHQALKAVLC ALFLQKPLEV TLSSTYSAPY LQLPKSKAAL YSCNETARLL WNTHAAPSLA DNSLEGEVEW LEWESTVLTP ALTPLYTQRR ITSEAVAALK KLDSTIEEHQ GTVAVKGLPS SKSFLVDSVL FASLLPALCE GGLLPATEAA AVPYLIKWFQ AFQAEHAELI ASAFDVLSVQ EYGDFLRVPR TYQVSPKKQK TFFATSPIYY VNASPHIGHV YSTLIVDVLG RYHRVKGEEV FVMTGTDEHG QKVAEAAAKQ GVSTMDFTTS VSNEFKQCFK EMNYDMNYFI RTTNPMHEKL VGDIWKKLEA KGDIYLGKYE GWYSVSDESF LTAQNVADSV DKDGNPCKIS LESGHVVTWV VEENYMFRLS AFRERLLKHF HDHPNCIVPE FRRREVIKTV EKGLFDLSIS RKCESVMNWS IPVPGDDRHC IYVWLDALFS YYVGSIVRVA ADGTEALDED YRTLSRWPAD LQVVGKDILK FHAIYWPAFL MSADLPLPER LVSHGWWTKD HKKISKSLGN AFDPVEKANE FGIDAFKYFL LRESNFQDDG DYSDRNMVAR LNGELADTLG NLVSRCVTPK INVGGVWPEP AEYNETDRTL IASLNNLAGT VDHYYCLPDI QNALIAIFDV LRSLNVYVTD NAPWKLVKTD TARLGTVLYV TMEGLRICNM FLQPVMPQKA KEIMDALGVP EGVRMDMENY PFGIVKPGTK IGGLAEGQVI FQKVTLPAEE GNAAQGDSKK AM
NT Sequence
atgatgttga ggttctttac cgataaggcg aatcaccagg ccctcaaggc tgtgctttgt gccttgtttc tgcagaagcc cctcgaggtg actctgagca gtacctacag cgcgccgtac cttcagctgc ccaagtcgaa ggcggccctg tacagctgca atgagacagc gcgtcttctc tggaacacac acgccgctcc atcgctcgct gacaattcgc ttgaaggaga ggtggaatgg ctcgagtggg agtcgacggt gctgactcca gccttgaccc ccctctacac acagcgacgc atcaccagcg aggctgtggc tgccctgaag aagctcgact ccaccattga ggagcaccaa ggcaccgtgg cggtaaaggg acttccgtcg tccaagtctt tcctagtgga tagcgtcctc ttcgcctccc tcctgccggc gctgtgcgag ggtgggctgc tccccgccac ggaggcagcg gcggtaccgt atctaattaa gtggttccag gccttccagg cagagcacgc ggagctgatc gcctctgcgt ttgacgtgct gtcggtgcaa gagtacgggg acttcttgcg ggtgccacgc acgtaccagg tgagccccaa gaagcagaag accttcttcg ccacatcgcc gatctactac gtgaacgcgt cgccacacat cggccacgtc tacagtacgc ttatcgttga cgtgctcggc cgctaccacc gagtgaaggg ggaggaagtg ttcgtaatga cgggcacgga cgagcacggt cagaaggtgg ccgaggcggc ggcgaagcag ggggtgtcga cgatggactt cacgacgagc gtgtcgaacg agtttaagca gtgcttcaag gagatgaatt acgacatgaa ttacttcatc cgcaccacaa acccgatgca tgagaagctg gtgggggaca tctggaagaa actggaggcg aagggcgaca tctacctcgg caagtacgag ggctggtact cggtgagcga cgagtcgttc ttgacggcgc agaacgtggc ggacagtgta gacaaggatg gaaatccctg caagatcagt ctggagagcg gccacgtcgt gacgtgggtg gtggaggaga actacatgtt ccgccttagc gcgttccgcg agcgtctcct caagcacttc catgaccacc ccaactgcat tgtcccggag ttccgccgcc gcgaggtgat caagacggtc gagaaggggc tcttcgactt gtccatctcc cgtaagtgcg agtccgtcat gaactggtcc atcccagtcc ccggtgatga tcgccactgc atctacgtgt ggctggacgc cctcttcagc tattacgtcg gctccattgt ccgtgtcgcg gccgacggca ccgaggccct ggacgaggac taccgcacac tgagccgctg gccggccgac ctgcaggtgg ttgggaagga cattctcaag ttccacgcta tttactggcc ggccttcttg atgtccgctg acttgccgct gccggagcgg ttggtgtcac atggctggtg gacgaaagat cacaagaaga tcagcaagtc gctcggcaac gccttcgacc cagtggaaaa ggcgaacgag ttcggcatcg acgcgttcaa gtacttcctg ctacgcgagt caaacttcca ggatgatggc gattacagcg ataggaacat ggtagcccgc ctgaacggcg aactcgccga cacgctcgga aacctggtgt cgcgctgcgt gacgccaaag atcaatgtgg gtggtgtgtg gccggagcct gcagaataca acgagactga caggacgctg attgcctcgc tcaacaatct ggctggcacg gtggatcact actactgcct gcctgacatc cagaacgcgt tgattgccat cttcgacgtg ctccgcagcc tcaacgtcta cgtgaccgat aatgcgccgt ggaagttggt gaagacggac acggcgcgcc tcggcacggt gctgtacgtg acgatggagg ggctgcgcat ctgcaatatg ttcctgcagc cggtgatgcc gcagaaggca aaggagatca tggacgcgct tggggtgccg gagggggtgc gcatggatat ggagaactac ccgttcggca ttgtgaagcc ggggacgaag attggcggct tggcggaggg ccaggtcatt ttccaaaagg tgacactccc agccgaggag gggaatgcgg cacaggggga cagcaagaag gccatgtag
Details for LebrA.10201.a.B2.GE42662
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 81kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAP YLQLPKSKAA LYSCNETARL LWNTHAAPSL ADNSLEGEVE WLEWESTVLT PALTPLYTQR RITSEAVAAL KKLDSTIEEH QGTVAVKGLP SSKSFLVDSV LFASLLPALC EGGLLPATEA AAVPYLIKWF QAFQAEHAEL IASAFDVLSV QEYGDFLRVP RTYQVSPKKQ KTFFATSPIY YVNASPHIGH VYSTLIVDVL GRYHRVKGEE VFVMTGTDEH GQKVAEAAAK QGVSTMDFTT SVSNEFKQCF KEMNYDMNYF IRTTNPMHEK LVGDIWKKLE AKGDIYLGKY EGWYSVSDES FLTAQNVADS VDKDGNPCKI SLESGHVVTW VVEENYMFRL SAFRERLLKH FHDHPNCIVP EFRRREVIKT VEKGLFDLSI SRKCESVMNW SIPVPGDDRH CIYVWLDALF SYYVGSIVRV AADGTEALDE DYRTLSRWPA DLQVVGKDIL KFHAIYWPAF LMSADLPLPE RLVSHGWWTK DHKKISKSLG NAFDPVEKAN EFGIDAFKYF LLRESNFQDD GDYSDRNMVA RLNGELADTL GNLVSRCVTP KINVGGVWPE PAEYNETDRT LIASLNNLAG TVDHYYCLPD IQNALIAIFD VLRSLNVYVT DNAPWKLVKT DTARLGTVLY VTMEGLRICN MFLQPVMPQK AKEIMDALGV PEGVRMDMEN YPFGIVKPGT KIGGLAEGQV IFQKVTLPAE EGNAAQGDSK KAM
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgccgt accttcagct gcccaagtcg aaggcggccc tgtacagctg caatgagaca gcgcgtcttc tctggaacac acacgccgct ccatcgctcg ctgacaattc gcttgaagga gaggtggaat ggctcgagtg ggagtcgacg gtgctgactc cagccttgac ccccctctac acacagcgac gcatcaccag cgaggctgtg gctgccctga agaagctcga ctccaccatt gaggagcacc aaggcaccgt ggcggtaaag ggacttccgt cgtccaagtc tttcctagtg gatagcgtcc tcttcgcctc cctcctgccg gcgctgtgcg agggtgggct gctccccgcc acggaggcag cggcggtacc gtatctaatt aagtggttcc aggccttcca ggcagagcac gcggagctga tcgcctctgc gtttgacgtg ctgtcggtgc aagagtacgg ggacttcttg cgggtgccac gcacgtacca ggtgagcccc aagaagcaga agaccttctt cgccacatcg ccgatctact acgtgaacgc gtcgccacac atcggccacg tctacagtac gcttatcgtt gacgtgctcg gccgctacca ccgagtgaag ggggaggaag tgttcgtaat gacgggcacg gacgagcacg gtcagaaggt ggccgaggcg gcggcgaagc agggggtgtc gacgatggac ttcacgacga gcgtgtcgaa cgagtttaag cagtgcttca aggagatgaa ttacgacatg aattacttca tccgcaccac aaacccgatg catgagaagc tggtggggga catctggaag aaactggagg cgaagggcga catctacctc ggcaagtacg agggctggta ctcggtgagc gacgagtcgt tcttgacggc gcagaacgtg gcggacagtg tagacaagga tggaaatccc tgcaagatca gtctggagag cggccacgtc gtgacgtggg tggtggagga gaactacatg ttccgcctta gcgcgttccg cgagcgtctc ctcaagcact tccatgacca ccccaactgc attgtcccgg agttccgccg ccgcgaggtg atcaagacgg tcgagaaggg gctcttcgac ttgtccatct cccgtaagtg cgagtccgtc atgaactggt ccatcccagt ccccggtgat gatcgccact gcatctacgt gtggctggac gccctcttca gctattacgt cggctccatt gtccgtgtcg cggccgacgg caccgaggcc ctggacgagg actaccgcac actgagccgc tggccggccg acctgcaggt ggttgggaag gacattctca agttccacgc tatttactgg ccggccttct tgatgtccgc tgacttgccg ctgccggagc ggttggtgtc acatggctgg tggacgaaag atcacaagaa gatcagcaag tcgctcggca acgccttcga cccagtggaa aaggcgaacg agttcggcat cgacgcgttc aagtacttcc tgctacgcga gtcaaacttc caggatgatg gcgattacag cgataggaac atggtagccc gcctgaacgg cgaactcgcc gacacgctcg gaaacctggt gtcgcgctgc gtgacgccaa agatcaatgt gggtggtgtg tggccggagc ctgcagaata caacgagact gacaggacgc tgattgcctc gctcaacaat ctggctggca cggtggatca ctactactgc ctgcctgaca tccagaacgc gttgattgcc atcttcgacg tgctccgcag cctcaacgtc tacgtgaccg ataatgcgcc gtggaagttg gtgaagacgg acacggcgcg cctcggcacg gtgctgtacg tgacgatgga ggggctgcgc atctgcaata tgttcctgca gccggtgatg ccgcagaagg caaaggagat catggacgcg cttggggtgc cggagggggt gcgcatggat atggagaact acccgttcgg cattgtgaag ccggggacga agattggcgg cttggcggag ggccaggtca ttttccaaaa ggtgacactc ccagccgagg aggggaatgc ggcacagggg gacagcaaga aggccatgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat