LebrA.01510.a

Repressor of differentiation kinase 2, putative (RDK2)

CENTER ID: LebrA.01510.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.01510.a.B2.GE42787 PHoranyi kinase domain truncation 33 441
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.31.3330
UniProt: A4HJT5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEDPDQRKPN GGNNGLPVPP STTETGKDPL QGILGRMPDC QYVKKRLLGQ GSFGSAWRVE ESATGSVFAA KVMDLNSMSE KDRGFVTNEV KCLSRSRNPH IVRCENAIER EGHLLIVMEY ADGGDLYKQI KARVREMKFF REHEVVFIFL QLCLALDHIH SNKMMHRDLK TANILLTTTG LVKLGDFGFS RQYEDSLSNA VGSTFCGTPY YLSPELWHRQ PYSKKSEMWA LGVVLYEVMA LRRPFTGKNM DELITNICHG RRADLPPSYT ASLRDVCNRL LSVDANMRPT IQELFHEPFI RSNLETLKRS VEKHNRIPAD VRDAIARCIT QSLSTEEPPA QLEEPSPFIG TVSRHTALRG WQKCLLSFDK KQISIRDPSG VDPEEVVPVA TLTSVCPIDV MMAGEEFVFA MKNQAGKAFW FMAPNQRAYE QWLSTLQEAA P
NT Sequence
atggaggatc cagaccagcg aaagccgaac ggcggcaata acggtctgcc agtgccaccc agcacaacag agaccggtaa ggaccctctg cagggcatcc tcggtcggat gccggactgc caatatgtca agaagcgttt gctgggacag ggcagctttg gtagcgcgtg gcgtgtcgag gagagcgcga ccggcagcgt cttcgccgcg aaggtgatgg acctgaatag tatgagcgag aaggatcgtg gctttgtgac caacgaggtg aagtgtctct cgcgctcccg caacccgcac atcgtgcgct gcgagaacgc gatcgagcga gagggacact tgctgattgt gatggagtac gccgacggtg gagatctgta caagcagatc aaggctcgcg tgcgcgagat gaagttcttc cgggagcacg aggtggtgtt catctttcta cagctgtgcc ttgccctgga ccacatccac tcgaacaaga tgatgcaccg cgacctgaaa accgccaaca tcctcctcac taccaccggc ctcgtcaaac tgggcgactt tgggttttca cgccagtacg aggactccct ttccaatgcc gtcggctcga cgttctgcgg cacgccgtat tacctcagcc cggagttgtg gcaccgccag ccgtacagca aaaaaagtga gatgtgggcc ctgggcgttg tgctctacga agtcatggca ctccgccgcc ccttcaccgg caagaacatg gatgagctca tcacgaacat ttgccacggc cggcgtgctg acctgccccc atcgtacacg gcgagtcttc gcgatgtgtg taaccggctc ctgtccgtcg acgccaatat gcggccaacg atccaggaac tcttccacga gccctttatc cgctccaacc tcgagactct caagcgcagc gttgagaagc acaaccgcat ccccgccgac gttcgggacg ccatcgcacg ctgcattaca cagtcactga gcacggagga gccccctgcg cagttggagg agccgtcgcc attcattggc accgtaagtc gccacacggc gctgcgcggc tggcagaaat gtctcttgag cttcgacaag aagcagattt cgatccgtga cccaagcggg gtggatccgg aagaagtggt gccggttgct accctcacct ccgtctgccc catcgatgtg atgatggctg gcgaagagtt cgtctttgcc atgaagaacc aggccggtaa ggccttttgg ttcatggcgc caaaccagag ggcatacgag cagtggctga gtacgttgca ggaagctgcc ccgtaa
Details for LebrA.01510.a.B2.GE42787
HARVESTED ON: 1/9/2019
SEQUENCED ON: 1/15/2019
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMI LGRMPDCQYV KKRLLGQGSF GSAWRVEESA TGSVFAAKVM DLNSMSEKDR GFVTNEVKCL SRSRNPHIVR CENAIEREGH LLIVMEYADG GDLYKQIKAR VREMKFFREH EVVFIFLQLC LALDHIHSNK MMHRDLKTAN ILLTTTGLVK LGDFGFSRQY EDSLSNAVGS TFCGTPYYLS PELWHRQPYS KKSEMWALGV VLYEVMALRR PFTGKNMDEL ITNICHGRRA DLPPSYTASL RDVCNRLLSV DANMRPTIQE LFHEPFIRSN LETLKRSVEK HNRIPADVRD AIARCITQSL STEEPPAQLE EPSPFIGTVS RHTALRGWQK CLLSFDKKQI SIRDPSGVDP EEVVPVATLT SVCPIDVMMA GEEFVFAMKN QAGKAFWFMA PNQKAYEQW
Validated NT Sequence
cagccactgc tcgtatgcct tctggtttgg cgccatgaac caaaaggcct tgccggcctg gttcttcata gcaaagacga actcttcgcc agccatcatc acatcgatag ggcagacgga ggtgagggta gcaaccggca ccacttcttc cggatccacc ccgcttgggt cacggatcga aatctgcttc ttgtcgaagc tcaagagaca tttctgccag ccgcgcagcg ccgtgtggcg acttacggtg ccaatgaatg gcgacggctc ctccaactgc gcagggggct cctccgtgct cagtgactgt gtaatgcagc gtgcaatggc gtcccgaacg tcggcgggga tgcggttgtg cttctcaacg ctgcgcttga gagtctcgag gttggagcgg ataaagggct cgtggaagag ttcctggatt gttggccgca tattggcgtc gacggacagg agccggttac acacatcgcg aagactcgcc gtgtacgatg gcggcaggtc agcacgccgg ccgtggcaaa tgttcgtgat gagctcatcc atgttcttgc cggtgaaggg gcggcggagt gccatgactt cgtagagcac gacgccgagg gcccacatct cacttttttt gctgtacggc tggcggtgcc acaactccgg gctgaggtaa tacggcgtgc cacagaacgt cgagccgacg gcattggaaa gggagtcctc gtactggcgt gaaaaaccaa agtcgcccag tttgacgagg ccggtggtag tgaggagaat gttggcggtt ttcaggtcgc ggtgcatcat cttgttcgag tggatgtggt ccagggcaag gcacagctgt agaaagatga acaccacctc gtgctcccgg aagaacttca tctcgcgcac gcgagcctta atctgcttgt acagatctcc accgtcggcg tactccatca caatcagcaa gtgtccctct cgctcgatcg cgttctcgca gcgcacgatg tgcgggttgc gggagcgcga gagacacttc acctcgttgg tcacaaagcc acgatccttc tcgctcatgc tattcaggtc catcaccttt gcggcgaaga cgctgccggt cgcgctctcc tcgacacgcc acgcgctacc aaagctgccc tgtcccagca aacgcttctt gacatattgg cagtccggca tccgaccgag gatcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHIL GRMPDCQYVK KRLLGQGSFG SAWRVEESAT GSVFAAKVMD LNSMSEKDRG FVTNEVKCLS RSRNPHIVRC ENAIEREGHL LIVMEYADGG DLYKQIKARV REMKFFREHE VVFIFLQLCL ALDHIHSNKM MHRDLKTANI LLTTTGLVKL GDFGFSRQYE DSLSNAVGST FCGTPYYLSP ELWHRQPYSK KSEMWALGVV LYEVMALRRP FTGKNMDELI TNICHGRRAD LPPSYTASLR DVCNRLLSVD ANMRPTIQEL FHEPFIRSNL ETLKRSVEKH NRIPADVRDA IARCITQSLS TEEPPAQLEE PSPFIGTVSR HTALRGWQKC LLSFDKKQIS IRDPSGVDPE EVVPVATLTS VCPIDVMMAG EEFVFAMKNQ AGKAFWFMAP NQRAYEQWLS TLQEAAP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatcctcg gtcggatgcc ggactgccaa tatgtcaaga agcgtttgct gggacagggc agctttggta gcgcgtggcg tgtcgaggag agcgcgaccg gcagcgtctt cgccgcgaag gtgatggacc tgaatagtat gagcgagaag gatcgtggct ttgtgaccaa cgaggtgaag tgtctctcgc gctcccgcaa cccgcacatc gtgcgctgcg agaacgcgat cgagcgagag ggacacttgc tgattgtgat ggagtacgcc gacggtggag atctgtacaa gcagatcaag gctcgcgtgc gcgagatgaa gttcttccgg gagcacgagg tggtgttcat ctttctacag ctgtgccttg ccctggacca catccactcg aacaagatga tgcaccgcga cctgaaaacc gccaacatcc tcctcactac caccggcctc gtcaaactgg gcgactttgg gttttcacgc cagtacgagg actccctttc caatgccgtc ggctcgacgt tctgcggcac gccgtattac ctcagcccgg agttgtggca ccgccagccg tacagcaaaa aaagtgagat gtgggccctg ggcgttgtgc tctacgaagt catggcactc cgccgcccct tcaccggcaa gaacatggat gagctcatca cgaacatttg ccacggccgg cgtgctgacc tgcccccatc gtacacggcg agtcttcgcg atgtgtgtaa ccggctcctg tccgtcgacg ccaatatgcg gccaacgatc caggaactct tccacgagcc ctttatccgc tccaacctcg agactctcaa gcgcagcgtt gagaagcaca accgcatccc cgccgacgtt cgggacgcca tcgcacgctg cattacacag tcactgagca cggaggagcc ccctgcgcag ttggaggagc cgtcgccatt cattggcacc gtaagtcgcc acacggcgct gcgcggctgg cagaaatgtc tcttgagctt cgacaagaag cagatttcga tccgtgaccc aagcggggtg gatccggaag aagtggtgcc ggttgctacc ctcacctccg tctgccccat cgatgtgatg atggctggcg aagagttcgt ctttgccatg aagaaccagg ccggtaaggc cttttggttc atggcgccaa accagagggc atacgagcag tggctgagta cgttgcagga agctgccccg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat