LebrA.01480.a

Protein kinase domain-containing protein

CENTER ID: LebrA.01480.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.01480.a.HZ2.GE43704 kinase domain 124 662
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.31.3220
UniProt: A4HJS4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEAAPVAAAQ QPPQAQKRPR EESDASPQEG SNDSVPATFS NEHSLTTPVV GAELVVDVDQ SETNAQASAV DAPRSRSRGL PAVGPGPISP VAASRSGTEG ASSCQAAAVV ATLGSSSSAF ADARRFKQPT LAAYGMVSDT IALKKEISDR DSHIEELNRE VEGLRAEVGR RDDQIHFMDG QCRQYQKKLE HFQSVMRQEM LSAAQKDRSE ARRVLYQKHF ELGQTATWHS NGQTVWMEGD RVRTLFLTLA SLTQKREEAE ALKKAAQSSV KHLARQERER EDSGAGADLQ DALMAAQMEY QLRTSEHAAL TNSIADVKLK QAEVEHEKKA FVKEMRRVND EDTSEFLAIP TIGEGDRYVL MHLLGKGGFS EVWKAFDLVE GRYVACKIHH IQREWPTQTR THYLRHAQRE LDIMRALDHP HLTHLYDVFP RGDNMFISVM EYSNGMDLDT YLKRYRTFKE ADARLIFLQV VDVLRYLASL ESPIIHYDLK PANILLHRDD PTILDIKITD FGLSKIIGAT REGPSDNPSI ELTSQGTGTY WYLPPECFET ASTPRISNKV DIWSAGVIFY QMLFGRRPFA EGESQRRIWQ EKLIIQSART LDFPDAPKVS EEAKDVIRAC LAFNEHERCD VFQLSQDPYL FRTSRRSTHK AKSAALPGGG NSCSPLMPPV ADATPAS
NT Sequence
atggaagctg cacccgtggc ggcggcgcag caaccaccgc aggcccaaaa gcggccccgc gaggaaagcg atgcttctcc acaggagggc agcaacgaca gcgtgcccgc caccttctca aacgagcact ccctcacgac gccagttgtg ggcgcggagc ttgttgtgga cgtggatcag tcagagacga acgcacaggc gtcggccgta gatgccccgc gttcacgatc gcgtgggcta cccgccgtag ggccaggtcc catctcacct gtcgcggcct ctcgcagcgg tactgagggc gccagcagct gccaggcggc tgcagtggtc gcaacgctgg ggtcgtcctc ttcggccttt gcggacgcgc gccgcttcaa gcagcccacc cttgcggcct acggcatggt cagcgacacg atagcgctca agaaggagat tagcgaccgc gacagccaca ttgaggagtt gaaccgcgag gtggaggggc tgcgtgcgga ggtggggcgg cgcgatgacc agatacactt catggacggt caatgccggc agtaccagaa gaagctggag cacttccagt ccgtcatgcg gcaggagatg ctgagcgctg ctcagaagga tcgttcagag gctcgacgcg tcctctacca gaagcacttc gagctcggcc aaacggcgac gtggcacagc aatggccaga cggtatggat ggagggcgat cgggtgcgca cactctttct gacgctggcg agcctcaccc aaaaacgcga ggaagccgag gcgctcaaga aggcggcgca gagcagcgtg aagcatctcg cccggcagga gcgggagcgg gaggactcgg gcgccggcgc cgacctgcag gacgcgctga tggctgccca gatggagtat cagctgcgca cctctgagca cgccgcgctg acgaacagca tcgctgacgt caagctgaag caggcggagg tggagcacga aaagaaggca ttcgtaaagg agatgcgacg ggtgaatgac gaggacacgt ccgagttctt ggctatcccc accatcggcg agggagaccg ctacgtcctc atgcatctac taggcaaggg cggcttctct gaagtgtgga aggcgttcga cctcgtcgaa gggcgctatg tagcgtgtaa gattcaccac atccagcgcg agtggcctac ccagacgcgc actcactacc tgcgccatgc gcagcgggag ctcgacatta tgcgcgcgct cgatcacccg cacctgacac acctgtacga cgtcttcccc cgcggcgaca acatgttcat ttctgtgatg gagtacagca acggcatgga cctcgacacc tacctgaagc gctaccgcac cttcaaggag gccgacgcgc gactcatctt tctccaagtc gtggacgtac tgcgctacct cgcctccctg gagagcccca tcatccacta cgaccttaag ccggccaaca tactgctgca ccgcgacgac ccgaccatcc tcgacatcaa gattaccgac tttgggctct ccaagatcat cggcgccaca cgcgaaggcc ctagtgacaa cccgtccatc gagctcacct cgcagggcac cggcacctac tggtacctgc cgcccgagtg cttcgaaacc gcttcgacgc cgcgcatcag caacaaagtg gacatctggt ccgccggtgt tattttctac caaatgctct tcggcaggcg accgtttgcc gagggcgagt cgcagcgccg catatggcag gagaagctca tcattcaatc agcgcgtacg ctcgactttc cagacgcgcc caaggtgagt gaggaggcga aggacgtcat ccgcgcctgc ctcgccttca acgagcacga gcgatgcgac gtgtttcagc tgagtcagga cccctacctc tttcgaacga gccgccgctc cactcacaag gcaaagagcg ccgctctccc tggcggcgga aactcgtgtt cgccgctgat gccgcccgtc gccgacgcaa caccggcatc atag
Details for LebrA.01480.a.HZ2.GE43704
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 76kDa
OBSERVED MW: 75kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSRR FKQPTLAAYG MVSDTIALKK EISDRDSHIE ELNREVEGLR AEVGRRDDQI HFMDGQCRQY QKKLEHFQSV MRQEMLSAAQ KDRSEARRVL YQKHFELGQT ATWHSNGQTV WMEGDRVRTL FLTLASLTQK REEAEALKKA AQSSVKHLAR QEREREDSGA GADLQDALMA AQMEYQLRTS EHAALTNSIA DVKLKQAEVE HEKKAFVKEM RRVNDEDTSE FLAIPTIGEG DRYVLMHLLG KGGFSEVWKA FDLVEGRYVA CKIHHIQREW PTQTRTHYLR HAQRELDIMR ALDHPHLTHL YDVFPRGDNM FISVMEYSNG MDLDTYLKRY RTFKEADARL IFLQVVDVLR YLASLESPII HYDLKPANIL LHRDDPTILD IKITDFGLSK IIGATREGPS DNPSIELTSQ GTGTYWYLPP ECFETASTPR ISNKVDIWSA GVIFYQMLFG RRPFAEGESQ RRIWQEKLII QSARTLDFPD APKVSEEAKD VIRACLAFNE HERCDVFQLS QDPYLFRTSR RSTHKAKSAA LPGGGNS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcgc gccgcttcaa gcagcccacc cttgcggcct acggcatggt cagcgacacg atagcgctca agaaggagat tagcgaccgc gacagccaca ttgaggagtt gaaccgcgag gtggaggggc tgcgtgcgga ggtggggcgg cgcgatgacc agatacactt catggacggt caatgccggc agtaccagaa gaagctggag cacttccagt ccgtcatgcg gcaggagatg ctgagcgctg ctcagaagga tcgttcagag gctcgacgcg tcctctacca gaagcacttc gagctcggcc aaacggcgac gtggcacagc aatggccaga cggtatggat ggagggcgat cgggtgcgca cactctttct gacgctggcg agcctcaccc aaaaacgcga ggaagccgag gcgctcaaga aggcggcgca gagcagcgtg aagcatctcg cccggcagga gcgggagcgg gaggactcgg gcgccggcgc cgacctgcag gacgcgctga tggctgccca gatggagtat cagctgcgca cctctgagca cgccgcgctg acgaacagca tcgctgacgt caagctgaag caggcggagg tggagcacga aaagaaggca ttcgtaaagg agatgcgacg ggtgaatgac gaggacacgt ccgagttctt ggctatcccc accatcggcg agggagaccg ctacgtcctc atgcatctac taggcaaggg cggcttctct gaagtgtgga aggcgttcga cctcgtcgaa gggcgctatg tagcgtgtaa gattcaccac atccagcgcg agtggcctac ccagacgcgc actcactacc tgcgccatgc gcagcgggag ctcgacatta tgcgcgcgct cgatcacccg cacctgacac acctgtacga cgtcttcccc cgcggcgaca acatgttcat ttctgtgatg gagtacagca acggcatgga cctcgacacc tacctgaagc gctaccgcac cttcaaggag gccgacgcgc gactcatctt tctccaagtc gtggacgtac tgcgctacct cgcctccctg gagagcccca tcatccacta cgaccttaag ccggccaaca tactgctgca ccgcgacgac ccgaccatcc tcgacatcaa gattaccgac tttgggctct ccaagatcat cggcgccaca cgcgaaggcc ctagtgacaa cccgtccatc gagctcacct cgcagggcac cggcacctac tggtacctgc cgcccgagtg cttcgaaacc gcttcgacgc cgcgcatcag caacaaagtg gacatctggt ccgccggtgt tattttctac caaatgctct tcggcaggcg accgtttgcc gagggcgagt cgcagcgccg catatggcag gagaagctca tcattcaatc agcgcgtacg ctcgactttc cagacgcgcc caaggtgagt gaggaggcga aggacgtcat ccgcgcctgc ctcgccttca acgagcacga gcgatgcgac gtgtttcagc tgagtcagga cccctacctc tttcgaacga gccgccgctc cactcacaag gcaaagagcg ccgctctccc tggcggcgga aactcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa