LebrA.01238.a

seryl-tRNA synthetase

CENTER ID: LebrA.01238.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.01238.a.B1.GE42661 full length 1 474
LebrA.01238.a.B2.GE43043 XtalPred domain 1 404
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.01238.a.B1.PW38606 full length 1 474

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.19.0220
UniProt: A4H9U8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGLDIQLFRD PEMADVVRES ERRRYAKPEI VDKIIEVDKH WRRTQFLTEA SKKMINTCSK AVGAKKKAKE ADGDSSEVPA DVMSAAQEGT LDAQKLESLC ILQLKELSKA LTTQVEGLAK LAAEQEAERD RMVISVGNVL HESVPVSNDE NTGNVVVRTF GDVTRRMKMT HVDCMERLGL MDTSKTVTAM AGGRAFVLRG GLVQLQFALI SYALNFLIAR GYEPFYPPFF LNKEYMGAVA QLSEFDESLY KVSGDGDEKY LIATSEAPIA AYHTNKWFTE MNEPIKYAGV SSCFRKEAGA HGRDTLGIFR VHQFDKLEQF VVCSPREDQS WKMLEEMMQT SQDFNESLGL PYRVINICSG ALNNAAAKKY DLEAWFPGSG AFRELVSCSN CTDYQARSVN CRFGPNTKGS TANNTKEYCH MLNGTLCAVT RTMCCICENY QTEEGIVIPE VLRPFMMGTE MLKFPAEAPA GEKA
NT Sequence
atgggtctcg acatccagct tttccgcgat cccgagatgg cggatgtagt gcgcgagtcg gagcgccgcc gctacgccaa gccggagatc gttgataaaa ttatcgaagt cgacaagcac tggcgtcgca cgcagtttct aactgaagct agcaagaaga tgatcaacac ttgcagcaag gccgtcggtg ccaagaagaa ggccaaggag gcggacggcg acagctctga ggtgccggca gatgtcatga gcgcggccca ggagggcaca ctggacgcac agaagctgga gtcgttgtgc attcttcagc tgaaggagct ctccaaggca ctgacgacgc aggtggaggg acttgcaaag ctagccgcgg agcaagaagc ggaacgggat cgtatggtga tcagcgttgg caacgtcctg cacgagtcgg tgccggtttc gaacgacgag aatactggca acgtggtcgt gcgcaccttt ggtgatgtga cacgccgcat gaagatgaca catgtagact gcatggagcg cctgggcctc atggatacat ccaagacggt taccgcgatg gcgggcggcc gcgcttttgt tcttcgcggc ggtcttgtgc agctgcagtt cgctctcatc tcgtacgcgc tcaacttcct catcgcccgc ggctacgagc ctttctatcc gccgttcttc ctcaacaagg aatacatggg tgcagtggcg caactttcgg aattcgacga gtcactctac aaggtgtccg gtgacggaga cgagaagtac ctgattgcaa cgagcgaagc gcccattgct gcctatcaca ccaacaagtg gtttacagaa atgaatgaac cgattaagta cgcgggcgtc tcgtcgtgct tccgcaagga ggctggggcg cacggccgcg acactcttgg tatctttcgt gtccatcagt tcgacaaact tgagcagttt gtcgtatgct cacctcgcga ggatcagtcg tggaaaatgc tggaggagat gatgcagacc tcccaggact tcaacgagag ccttggtctg ccgtaccgcg tcatcaacat ctgttctgga gccctcaaca atgccgccgc caagaagtac gatctggagg catggttccc tggctccggt gcattccgcg agcttgtttc gtgctcgaat tgcacggact accaggcccg cagtgttaac tgccgcttcg gcccgaatac aaagggcagc acggcgaaca acacaaagga atactgccac atgctgaacg gcaccctctg cgctgtcact cgcacaatgt gctgcatctg cgaaaactac cagaccgagg aaggcatcgt catccccgag gttctgcgcc cctttatgat ggggacagag atgctcaagt tcccggcaga agctcctgcg ggcgagaagg cgtaa
Details for LebrA.01238.a.B1.GE42661
HARVESTED ON: 9/28/2018
SEQUENCED ON: 10/17/2018
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG LDIQLFRDPE MADVVRESER RRYAKPEIVD KIIEVDKHWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDSSEVPADV MSAAQEGTLD AQKLESLCIL QLKELSKALT TQVEGLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDENT GNVVVRTFGD VTRRMKMTHV DCMERLGLMD TSKTVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SEFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMN EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMMQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FGPNTKGSTA NNTKEYCHML NGTLCAVTRT MCCICENYQT EEGIVIPEVL RPFMMGTEML KFPA
Validated NT Sequence
tctgccggga acttgagcat ctctgtcccc atcataaagg ggcgcagaac ctcggggatg acgatgcctt cctcggtctg gtagttttcg cagatgcagc acattgtgcg agtgacagcg cagagggtgc cgttcagcat gtggcagtat tcctttgtgt tgttcgccgt gctgcccttt gtgttcgggc cgaagcggca gttaacactg cgggcctggt agtccgtgca attcgagcac gaaacaagct cgcggaatgc accggagcca gggaaccatg cctccagatc gtacttcttg gcggcggcat tgttgagggc gccagaacag atgttgatga cgcggtacgg cagaccaagg ctctcgttga agtcctggga ggtctgcatc atctcctcca gcattttcca cgactgatcc tcgcgcggtg agcatacgac aaactgctca agtttgtcga actgatggac acgaaagata ccaagagtgt cgcggccgtg cgccccagct tccttgcgga agcacgacga gacgcccgcg tacttaatcg gttcattcat ttctgtaaac cacttgttgg tgtgataggc agcaatgggc gcttcgctcg ttgcaatcag gtacttctcg tctccgtcac cggacacctt gtagagtgac tcgtcgaatt ccgaaagttg cgccactgca cccatgtatt ccttgttgag gaagaacggc ggatagaaag gctcgtagcc gcgggcgatg aggaagttga gcgcgtacga gatgagagcg aactgcagct gcacaagacc gccgcgaaga acaaaagcgc ggccgcccgc catcgccgta accgtcttgg atgtatccat gaggcccagg cgctccatgc agtctacatg tgtcatcttc atgcggcgtg tcacatcacc aaaggtgcgc acgaccacgt tgccagtatt ctcgtcgttt gaaaccggca ccgactcgtg caggacgttg ccaacgctga tcaccatacg atcccgttcc gcttcttgct ccgcggctag ctttgcaagt ccctccacct gcgtcgtcag tgccttggag agctccttca gctgaagaat gcacaacgac tccagcttct gtgcgtccag tgtgccctcc tgggccgcgc tcatgacatc tgccggcacc tcagagctgt cgccgtccgc ctccttggcc ttcttcttgg caccgacggc cttgctgcaa gtgttgatca tcttcttgct agcttcagtt agaaactgcg tgcgacgcca gtgcttgtcg acttcgataa ttttatcaac gatctccggc ttggcgtagc ggcggcgctc cgactcgcgc actacatccg ccatctcggg atcgcggaaa agctggatgt cgagacccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMG LDIQLFRDPE MADVVRESER RRYAKPEIVD KIIEVDKHWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDSSEVPADV MSAAQEGTLD AQKLESLCIL QLKELSKALT TQVEGLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDENT GNVVVRTFGD VTRRMKMTHV DCMERLGLMD TSKTVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SEFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMN EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMMQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FGPNTKGSTA NNTKEYCHML NGTLCAVTRT MCCICENYQT EEGIVIPEVL RPFMMGTEML KFPAEAPAGE KA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtc tcgacatcca gcttttccgc gatcccgaga tggcggatgt agtgcgcgag tcggagcgcc gccgctacgc caagccggag atcgttgata aaattatcga agtcgacaag cactggcgtc gcacgcagtt tctaactgaa gctagcaaga agatgatcaa cacttgcagc aaggccgtcg gtgccaagaa gaaggccaag gaggcggacg gcgacagctc tgaggtgccg gcagatgtca tgagcgcggc ccaggagggc acactggacg cacagaagct ggagtcgttg tgcattcttc agctgaagga gctctccaag gcactgacga cgcaggtgga gggacttgca aagctagccg cggagcaaga agcggaacgg gatcgtatgg tgatcagcgt tggcaacgtc ctgcacgagt cggtgccggt ttcgaacgac gagaatactg gcaacgtggt cgtgcgcacc tttggtgatg tgacacgccg catgaagatg acacatgtag actgcatgga gcgcctgggc ctcatggata catccaagac ggttaccgcg atggcgggcg gccgcgcttt tgttcttcgc ggcggtcttg tgcagctgca gttcgctctc atctcgtacg cgctcaactt cctcatcgcc cgcggctacg agcctttcta tccgccgttc ttcctcaaca aggaatacat gggtgcagtg gcgcaacttt cggaattcga cgagtcactc tacaaggtgt ccggtgacgg agacgagaag tacctgattg caacgagcga agcgcccatt gctgcctatc acaccaacaa gtggtttaca gaaatgaatg aaccgattaa gtacgcgggc gtctcgtcgt gcttccgcaa ggaggctggg gcgcacggcc gcgacactct tggtatcttt cgtgtccatc agttcgacaa acttgagcag tttgtcgtat gctcacctcg cgaggatcag tcgtggaaaa tgctggagga gatgatgcag acctcccagg acttcaacga gagccttggt ctgccgtacc gcgtcatcaa catctgttct ggagccctca acaatgccgc cgccaagaag tacgatctgg aggcatggtt ccctggctcc ggtgcattcc gcgagcttgt ttcgtgctcg aattgcacgg actaccaggc ccgcagtgtt aactgccgct tcggcccgaa tacaaagggc agcacggcga acaacacaaa ggaatactgc cacatgctga acggcaccct ctgcgctgtc actcgcacaa tgtgctgcat ctgcgaaaac taccagaccg aggaaggcat cgtcatcccc gaggttctgc gcccctttat gatggggaca gagatgctca agttcccggc agaagctcct gcgggcgaga aggcgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LebrA.01238.a.B2.GE43043
HARVESTED ON: 4/19/2019
SEQUENCED ON: 4/23/2019
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMG LDIQLFRDPE MADVVRESER RRYAKPEIVD KIIEVDKHWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDSSEVPADV MSAAQEGTLD AQKLESLCIL QLKELSKALT TQVEGLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDENT GNVVVRTFGD VTRRMKMTHV DCMERLGLMD TSKTVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SEFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMN EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMMQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQ
Validated NT Sequence
ggcctggtag tccgtgcaat tcgagcacga aacaagctcg cggaatgcac cggagccagg gaaccatgcc tccagatcgt acttcttggc ggcggcattg ttgagggcgc cagaacagat gttgatgacg cggtacggca gaccaaggct ctcgttgaag tcctgggagg tctgcatcat ctcctccagc attttccacg actgatcctc gcgcggtgag catacgacaa actgctcaag tttgtcgaac tgatggacac gaaagatacc aagagtgtcg cggccgtgcg ccccagcttc cttgcggaag cacgacgaga cgcccgcgta cttaatcggt tcattcattt ctgtaaacca cttgttggtg tgataggcag caatgggcgc ttcgctcgtt gcaatcaggt acttctcgtc tccgtcaccg gacaccttgt agagtgactc gtcgaattcc gaaagttgcg ccactgcacc catgtattcc ttgttgagga agaacggcgg atagaaaggc tcgtagccgc gggcgatgag gaagttgagc gcgtacgaga tgagagcgaa ctgcagctgc acaagaccgc cgcgaagaac aaaagcgcgg ccgcccgcca tcgccgtaac cgtcttggat gtatccatga ggcccaggcg ctccatgcag tctacatgtg tcatcttcat gcggcgtgtc acatcaccaa aggtgcgcac gaccacgttg ccagtattct cgtcgtttga aaccggcacc gactcgtgca ggacgttgcc aacgctgatc accatacgat cccgttccgc ttcttgctcc gcggctagct ttgcaagtcc ctccacctgc gtcgtcagtg ccttggagag ctccttcagc tgaagaatgc acaacgactc cagcttctgt gcgtccagtg tgccctcctg ggccgcgctc atgacatctg ccggcacctc agagctgtcg ccgtccgcct ccttggcctt cttcttggca ccgacggcct tgctgcaagt gttgatcatc ttcttgctag cttcagttag aaactgcgtg cgacgccagt gcttgtcgac ttcgataatt ttatcaacga tctccggctt ggcgtagcgg cggcgctccg actcgcgcac tacatccgcc atctcgggat cgcggaaaag ctggatgtcg agacccatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMG LDIQLFRDPE MADVVRESER RRYAKPEIVD KIIEVDKHWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDSSEVPADV MSAAQEGTLD AQKLESLCIL QLKELSKALT TQVEGLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDENT GNVVVRTFGD VTRRMKMTHV DCMERLGLMD TSKTVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SEFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMN EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMMQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggtc tcgacatcca gcttttccgc gatcccgaga tggcggatgt agtgcgcgag tcggagcgcc gccgctacgc caagccggag atcgttgata aaattatcga agtcgacaag cactggcgtc gcacgcagtt tctaactgaa gctagcaaga agatgatcaa cacttgcagc aaggccgtcg gtgccaagaa gaaggccaag gaggcggacg gcgacagctc tgaggtgccg gcagatgtca tgagcgcggc ccaggagggc acactggacg cacagaagct ggagtcgttg tgcattcttc agctgaagga gctctccaag gcactgacga cgcaggtgga gggacttgca aagctagccg cggagcaaga agcggaacgg gatcgtatgg tgatcagcgt tggcaacgtc ctgcacgagt cggtgccggt ttcgaacgac gagaatactg gcaacgtggt cgtgcgcacc tttggtgatg tgacacgccg catgaagatg acacatgtag actgcatgga gcgcctgggc ctcatggata catccaagac ggttaccgcg atggcgggcg gccgcgcttt tgttcttcgc ggcggtcttg tgcagctgca gttcgctctc atctcgtacg cgctcaactt cctcatcgcc cgcggctacg agcctttcta tccgccgttc ttcctcaaca aggaatacat gggtgcagtg gcgcaacttt cggaattcga cgagtcactc tacaaggtgt ccggtgacgg agacgagaag tacctgattg caacgagcga agcgcccatt gctgcctatc acaccaacaa gtggtttaca gaaatgaatg aaccgattaa gtacgcgggc gtctcgtcgt gcttccgcaa ggaggctggg gcgcacggcc gcgacactct tggtatcttt cgtgtccatc agttcgacaa acttgagcag tttgtcgtat gctcacctcg cgaggatcag tcgtggaaaa tgctggagga gatgatgcag acctcccagg acttcaacga gagccttggt ctgccgtacc gcgtcatcaa catctgttct ggagccctca acaatgccgc cgccaagaag tacgatctgg aggcatggtt ccctggctcc ggtgcattcc gcgagcttgt ttcgtgctcg aattgcacgg actaccaggc ccgcagtgtt aactgccgct tcggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for LebrA.01238.a.B1.PW38606
PURIFICATION DATe: 4/24/2019
CONCENTRATION: 18.5mg/ml
OBSERVED MW: 55kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES 500 mM NaCl 5% Glycerol 2 mM DTT 0.025% Azide pH 7
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG LDIQLFRDPE MADVVRESER RRYAKPEIVD KIIEVDKHWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDSSEVPADV MSAAQEGTLD AQKLESLCIL QLKELSKALT TQVEGLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDENT GNVVVRTFGD VTRRMKMTHV DCMERLGLMD TSKTVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SEFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMN EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMMQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FGPNTKGSTA NNTKEYCHML NGTLCAVTRT MCCICENYQT EEGIVIPEVL RPFMMGTEML KFPA
Validated NT Sequence
tctgccggga acttgagcat ctctgtcccc atcataaagg ggcgcagaac ctcggggatg acgatgcctt cctcggtctg gtagttttcg cagatgcagc acattgtgcg agtgacagcg cagagggtgc cgttcagcat gtggcagtat tcctttgtgt tgttcgccgt gctgcccttt gtgttcgggc cgaagcggca gttaacactg cgggcctggt agtccgtgca attcgagcac gaaacaagct cgcggaatgc accggagcca gggaaccatg cctccagatc gtacttcttg gcggcggcat tgttgagggc gccagaacag atgttgatga cgcggtacgg cagaccaagg ctctcgttga agtcctggga ggtctgcatc atctcctcca gcattttcca cgactgatcc tcgcgcggtg agcatacgac aaactgctca agtttgtcga actgatggac acgaaagata ccaagagtgt cgcggccgtg cgccccagct tccttgcgga agcacgacga gacgcccgcg tacttaatcg gttcattcat ttctgtaaac cacttgttgg tgtgataggc agcaatgggc gcttcgctcg ttgcaatcag gtacttctcg tctccgtcac cggacacctt gtagagtgac tcgtcgaatt ccgaaagttg cgccactgca cccatgtatt ccttgttgag gaagaacggc ggatagaaag gctcgtagcc gcgggcgatg aggaagttga gcgcgtacga gatgagagcg aactgcagct gcacaagacc gccgcgaaga acaaaagcgc ggccgcccgc catcgccgta accgtcttgg atgtatccat gaggcccagg cgctccatgc agtctacatg tgtcatcttc atgcggcgtg tcacatcacc aaaggtgcgc acgaccacgt tgccagtatt ctcgtcgttt gaaaccggca ccgactcgtg caggacgttg ccaacgctga tcaccatacg atcccgttcc gcttcttgct ccgcggctag ctttgcaagt ccctccacct gcgtcgtcag tgccttggag agctccttca gctgaagaat gcacaacgac tccagcttct gtgcgtccag tgtgccctcc tgggccgcgc tcatgacatc tgccggcacc tcagagctgt cgccgtccgc ctccttggcc ttcttcttgg caccgacggc cttgctgcaa gtgttgatca tcttcttgct agcttcagtt agaaactgcg tgcgacgcca gtgcttgtcg acttcgataa ttttatcaac gatctccggc ttggcgtagc ggcggcgctc cgactcgcgc actacatccg ccatctcggg atcgcggaaa agctggatgt cgagacccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMG LDIQLFRDPE MADVVRESER RRYAKPEIVD KIIEVDKHWR RTQFLTEASK KMINTCSKAV GAKKKAKEAD GDSSEVPADV MSAAQEGTLD AQKLESLCIL QLKELSKALT TQVEGLAKLA AEQEAERDRM VISVGNVLHE SVPVSNDENT GNVVVRTFGD VTRRMKMTHV DCMERLGLMD TSKTVTAMAG GRAFVLRGGL VQLQFALISY ALNFLIARGY EPFYPPFFLN KEYMGAVAQL SEFDESLYKV SGDGDEKYLI ATSEAPIAAY HTNKWFTEMN EPIKYAGVSS CFRKEAGAHG RDTLGIFRVH QFDKLEQFVV CSPREDQSWK MLEEMMQTSQ DFNESLGLPY RVINICSGAL NNAAAKKYDL EAWFPGSGAF RELVSCSNCT DYQARSVNCR FGPNTKGSTA NNTKEYCHML NGTLCAVTRT MCCICENYQT EEGIVIPEVL RPFMMGTEML KFPAEAPAGE KA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGTC TCGACATCCA GCTTTTCCGC GATCCCGAGA TGGCGGATGT AGTGCGCGAG TCGGAGCGCC GCCGCTACGC CAAGCCGGAG ATCGTTGATA AAATTATCGA AGTCGACAAG CACTGGCGTC GCACGCAGTT TCTAACTGAA GCTAGCAAGA AGATGATCAA CACTTGCAGC AAGGCCGTCG GTGCCAAGAA GAAGGCCAAG GAGGCGGACG GCGACAGCTC TGAGGTGCCG GCAGATGTCA TGAGCGCGGC CCAGGAGGGC ACACTGGACG CACAGAAGCT GGAGTCGTTG TGCATTCTTC AGCTGAAGGA GCTCTCCAAG GCACTGACGA CGCAGGTGGA GGGACTTGCA AAGCTAGCCG CGGAGCAAGA AGCGGAACGG GATCGTATGG TGATCAGCGT TGGCAACGTC CTGCACGAGT CGGTGCCGGT TTCGAACGAC GAGAATACTG GCAACGTGGT CGTGCGCACC TTTGGTGATG TGACACGCCG CATGAAGATG ACACATGTAG ACTGCATGGA GCGCCTGGGC CTCATGGATA CATCCAAGAC GGTTACCGCG ATGGCGGGCG GCCGCGCTTT TGTTCTTCGC GGCGGTCTTG TGCAGCTGCA GTTCGCTCTC ATCTCGTACG CGCTCAACTT CCTCATCGCC CGCGGCTACG AGCCTTTCTA TCCGCCGTTC TTCCTCAACA AGGAATACAT GGGTGCAGTG GCGCAACTTT CGGAATTCGA CGAGTCACTC TACAAGGTGT CCGGTGACGG AGACGAGAAG TACCTGATTG CAACGAGCGA AGCGCCCATT GCTGCCTATC ACACCAACAA GTGGTTTACA GAAATGAATG AACCGATTAA GTACGCGGGC GTCTCGTCGT GCTTCCGCAA GGAGGCTGGG GCGCACGGCC GCGACACTCT TGGTATCTTT CGTGTCCATC AGTTCGACAA ACTTGAGCAG TTTGTCGTAT GCTCACCTCG CGAGGATCAG TCGTGGAAAA TGCTGGAGGA GATGATGCAG ACCTCCCAGG ACTTCAACGA GAGCCTTGGT CTGCCGTACC GCGTCATCAA CATCTGTTCT GGAGCCCTCA ACAATGCCGC CGCCAAGAAG TACGATCTGG AGGCATGGTT CCCTGGCTCC GGTGCATTCC GCGAGCTTGT TTCGTGCTCG AATTGCACGG ACTACCAGGC CCGCAGTGTT AACTGCCGCT TCGGCCCGAA TACAAAGGGC AGCACGGCGA ACAACACAAA GGAATACTGC CACATGCTGA ACGGCACCCT CTGCGCTGTC ACTCGCACAA TGTGCTGCAT CTGCGAAAAC TACCAGACCG AGGAAGGCAT CGTCATCCCC GAGGTTCTGC GCCCCTTTAT GATGGGGACA GAGATGCTCA AGTTCCCGGC AGAAGCTCCT GCGGGCGAGA AGGCGTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT