LebrA.00825.a

aspartyl-tRNA synthetase, putative

CENTER ID: LebrA.00825.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.00825.a.B1.GE42521 full length 1 550
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.30.0540
UniProt: A4HHZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSNHAEAKA PAVEKKISDK EARKAARLAE EKARAEEKAA LVEKYKDMFG AAPMVQSTAY MSHTYVPVAD LSQPALVDKT VLIRARVSTT RKKGKMAFMV LRDGRDSVQA MVAVEGDVPK EMIDFVGQIP TESIVDVEAS VCKVEQSITS TSHSDIELKV KKIHTVTESL RTLPFTLEDA NRKESDEGAK VNFDTRLNSR WMDLRTPASG AIFRLQSRVC QYFRQFLIDS DFCEIHSPKI INAPSEGGAN VFKLEYFNRF AYLAQSPQLY KQMVLQGDVP RVFEVGPVFR SENSNTHRHL TEFVGLDVEM RINEHYYEVL DLAESLFNYM FEHLATHTKE LKDVCQQYPF EPLVWKVTPE TMKELGVGVI SEGVEPTDKY QARVHNMDSR MLRMNYMHCI ELLNTVLEEK MAPTDDINTT NEKLLGKLVK ERYGTDFFIS DRFPSSARPF YTMECKDDAR FTNSYDMFIR GEEISSGAQR IHDPDLLLAR AKMLNVDLTP IKEYVDSFRL GAWPHGGFGV GLERVVMLYL GLGNVRLASL FPRDPQRTTP
NT Sequence
atgagctcca accacgccga ggctaaggca cccgcggtgg agaagaagat aagcgacaag gaggcccgca aggcggcgcg cctggctgag gagaaggccc gcgccgagga aaaggcggcc ctcgtggaga aatataaaga catgtttggt gctgcaccga tggtgcagtc gacggcgtac atgtcgcaca cgtacgttcc ggttgcagac ctgtcgcagc cggcgttggt agacaagacc gtgttgatcc gtgcccgggt ctcgacgacg cgcaagaagg ggaagatggc gtttatggtg ctgcgcgacg ggagggactc ggtgcaggcg atggttgctg tggagggcga cgtaccgaag gagatgattg acttcgtggg acagattccg acagagtcga ttgtggacgt cgaggcctcg gtatgcaagg tggagcagtc catcacatcg acgtcgcact cggacattga gctgaaggtg aagaaaattc acacagtgac ggagtcgctg cgcacgctgc cgtttacact agaggacgcg aaccgcaagg agtcggacga gggcgcgaag gtgaactttg acacgcgcct gaacagtcga tggatggatc tgcgcacgcc ggcgtccggc gcgatctttc gtcttcagtc gcgtgtgtgc cagtacttcc gccagtttct tatcgacagc gacttctgtg aaatccactc gcccaagatc atcaacgcgc cgagcgaagg tggcgccaat gtgttcaagc tggagtactt caaccgcttt gcatatctcg cccagtcgcc acagctctac aagcagatgg tgctacaagg tgatgtgcca cgcgtgttcg aagtgggacc ggtctttcgc tccgagaaca gcaacacaca ccgccacctg acggagttcg tcgggctgga cgtggagatg cgcattaatg agcactacta tgaggtgctg gatctagcag agagcctctt taactacatg ttcgagcacc tcgctacaca cacaaaggag ctgaaggatg tgtgccaaca gtacccgttt gagcctcttg tatggaaggt taccccagag acgatgaagg agcttggtgt tggcgtcatc tctgagggcg tggagccgac agacaagtac caggcacgcg tgcacaacat ggatagtcgt atgctgcgga tgaactacat gcactgcatc gagctgctga acactgtcct ggaggagaag atggcgccga ctgatgacat caacacgacg aacgaaaagc tccttggcaa gcttgtgaag gagcgctacg gcacggattt tttcatctca gatcgctttc cgtcctcggc acgccccttc tacacgatgg agtgcaagga tgacgcgcgc ttcacgaact cgtatgatat gttcatccgc ggagaggaga tctccagcgg agcgcagcgc attcacgatc ctgatttgct cctggcgcgc gccaagatgc tgaacgtcga cctcacgccg atcaaggagt atgtcgactc gttccgtctc ggtgcgtggc cgcatggcgg ctttggcgtt gggctggagc gcgtggtgat gctgtacctt gggctgggca acgtgcgcct tgcttcgctg tttccccgtg acccgcagcg cacgactccg tag
Details for LebrA.00825.a.B1.GE42521
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 64kDa
OBSERVED MW: 64kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMS SNHAEAKAPA VEKKISDKEA RKAARLAEEK ARAEEKAALV EKYKDMFGAA PMVQSTAYMS HTYVPVADLS QPALVDKTVL IRARVSTTRK KGKMAFMVLR DGRDSVQAMV AVEGDVPKEM IDFVGQIPTE SIVDVEASVC KVEQSITSTS HSDIELKVKK IHTVTESLRT LPFTLEDANR KESDEGAKVN FDTRLNSRWM DLRTPASGAI FRLQSRVCQY FRQFLIDSDF CEXXSPKXIN APSEGGANVF KLEYFNRFAY LXXSPQLYKQ MVLQGDVPRV FEVGPVFRSE NSNTHRHLTE FVGLDVEMRI NEHYYEVLDL AESLFNYMFE HLATHTKELK DVCQQYPFEP LVWKVTPETM KELGVGVISK GVEPTDKYQA RVHNMDSRML RMNYMHCIEL LNTVLEEKMA PTDDINTTNE KLLGKLVKER YGTDFFISDR FPSSARPFYT MECKDDARFT NSYDMFIRGE EISSGAQRIH DPDLLLARAK MLNVDLTPIK EYVDSFRLGA WPHGGFGVGL ERVVMLYLGL GNVRLASLFP
Validated NT Sequence
agatatacca tggctcacca ccaccaccac catatgagct ccaaccacgc cgaggctaag gcacccgcgg tggagaagaa gataagcgac aaggaggccc gcaaggcggc gcgcctggct gaggagaagg cccgcgccga ggaaaaggcg gccctcgtgg agaaatataa agacatgttt ggtgctgcac cgatggtgca gtcgacggcg tacatgtcgc acacgtacgt tccggttgca gacctgtcgc agccggcgtt ggtagacaag accgtgttga tccgtgcccg ggtctcgacg acgcgcaaga aggggaagat ggcgtttatg gtgctgcgcg acgggaggga ctcggtgcag gcgatggttg ctgtggaggg cgacgtaccg aaggagatga ttgacttcgt gggacagatt ccgacagagt cgattgtgga cgtcgaggcc tcggtatgca aggtggagca gtccatcaca tcgacgtcgc actcagacat tgagctgaag gtgaagaaaa ttcacacagt gacggagtcg ctgcgcacgc tgccgtttac actagaggac gcgaaccgca aggagtcgga cgagggtgcg aaggtgaact ttgacacgcg cctgaacagt cgatggatgg atctgcgcac gccggcgtcc ggcgcgatct ttcgtcttca gtcgcgtgtg tgccagtact tccgccagtt tcttatcgac agcgacttct gtgaannnnn ctcgcccaag ntnatcaacg cgccgagcga aggtggcgcc aatgtgttca agctggagta cttcaaccgc tttgcatatc tcnnnnagtc gccacagctc tacaagcaga tggtgctaca aggtgatgtg ccacgcgtgt tcgaagtggg accggtcttt cgctccgaga acagcaacac acaccgccac ctgacggagt tcgtcgggct ggacgtggag atgcgcatta atgagcacta ctatgaggtg ctggatctag cagagagcct ctttaactac atgttcgagc acctcgctac acacacaaag gagctgaagg atgtgtgcca acagtacccg tttgagcctc ttgtatggaa ggttacccca gagacgatga aggagcttgg tgttggcgtc atctctaagg gcgtggagcc gacagacaag taccaggcac gcgtgcacaa catggatagt cgtatgctgc ggatgaacta catgcactgc atcgagctgc tgaacactgt cctggaggag aagatggcgc cgactgatga catcaacacg acgaacgaaa agctccttgg caagcttgtg aaggagcgct acggcacgga ttttttcatc tcagatcgct ttccgtcctc ggcacgcccc ttctacacga tggagtgcaa ggatgacgcg cgcttcacga actcgtatga tatgttcatc cgcggagagg agatctccag cggagcgcag cgcattcacg atcctgattt actcctggcg cgcgccaaga tgctgaacgt cgacctcacg ccgatcaagg agtatgtcga ctcgttccgt ctcggtgcgt ggccgcatgg cggctttggc gttgggctgg agcgcgtggt gatgctgtac cttgggctgg gcaacgtgcg ccttgcttcg ctgtttcccc g
Expected Protein Sequence
MAHHHHHHMS SNHAEAKAPA VEKKISDKEA RKAARLAEEK ARAEEKAALV EKYKDMFGAA PMVQSTAYMS HTYVPVADLS QPALVDKTVL IRARVSTTRK KGKMAFMVLR DGRDSVQAMV AVEGDVPKEM IDFVGQIPTE SIVDVEASVC KVEQSITSTS HSDIELKVKK IHTVTESLRT LPFTLEDANR KESDEGAKVN FDTRLNSRWM DLRTPASGAI FRLQSRVCQY FRQFLIDSDF CEIHSPKIIN APSEGGANVF KLEYFNRFAY LAQSPQLYKQ MVLQGDVPRV FEVGPVFRSE NSNTHRHLTE FVGLDVEMRI NEHYYEVLDL AESLFNYMFE HLATHTKELK DVCQQYPFEP LVWKVTPETM KELGVGVISE GVEPTDKYQA RVHNMDSRML RMNYMHCIEL LNTVLEEKMA PTDDINTTNE KLLGKLVKER YGTDFFISDR FPSSARPFYT MECKDDARFT NSYDMFIRGE EISSGAQRIH DPDLLLARAK MLNVDLTPIK EYVDSFRLGA WPHGGFGVGL ERVVMLYLGL GNVRLASLFP RDPQRTTP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct ccaaccacgc cgaggctaag gcacccgcgg tggagaagaa gataagcgac aaggaggccc gcaaggcggc gcgcctggct gaggagaagg cccgcgccga ggaaaaggcg gccctcgtgg agaaatataa agacatgttt ggtgctgcac cgatggtgca gtcgacggcg tacatgtcgc acacgtacgt tccggttgca gacctgtcgc agccggcgtt ggtagacaag accgtgttga tccgtgcccg ggtctcgacg acgcgcaaga aggggaagat ggcgtttatg gtgctgcgcg acgggaggga ctcggtgcag gcgatggttg ctgtggaggg cgacgtaccg aaggagatga ttgacttcgt gggacagatt ccgacagagt cgattgtgga cgtcgaggcc tcggtatgca aggtggagca gtccatcaca tcgacgtcgc actcggacat tgagctgaag gtgaagaaaa ttcacacagt gacggagtcg ctgcgcacgc tgccgtttac actagaggac gcgaaccgca aggagtcgga cgagggcgcg aaggtgaact ttgacacgcg cctgaacagt cgatggatgg atctgcgcac gccggcgtcc ggcgcgatct ttcgtcttca gtcgcgtgtg tgccagtact tccgccagtt tcttatcgac agcgacttct gtgaaatcca ctcgcccaag atcatcaacg cgccgagcga aggtggcgcc aatgtgttca agctggagta cttcaaccgc tttgcatatc tcgcccagtc gccacagctc tacaagcaga tggtgctaca aggtgatgtg ccacgcgtgt tcgaagtggg accggtcttt cgctccgaga acagcaacac acaccgccac ctgacggagt tcgtcgggct ggacgtggag atgcgcatta atgagcacta ctatgaggtg ctggatctag cagagagcct ctttaactac atgttcgagc acctcgctac acacacaaag gagctgaagg atgtgtgcca acagtacccg tttgagcctc ttgtatggaa ggttacccca gagacgatga aggagcttgg tgttggcgtc atctctgagg gcgtggagcc gacagacaag taccaggcac gcgtgcacaa catggatagt cgtatgctgc ggatgaacta catgcactgc atcgagctgc tgaacactgt cctggaggag aagatggcgc cgactgatga catcaacacg acgaacgaaa agctccttgg caagcttgtg aaggagcgct acggcacgga ttttttcatc tcagatcgct ttccgtcctc ggcacgcccc ttctacacga tggagtgcaa ggatgacgcg cgcttcacga actcgtatga tatgttcatc cgcggagagg agatctccag cggagcgcag cgcattcacg atcctgattt gctcctggcg cgcgccaaga tgctgaacgt cgacctcacg ccgatcaagg agtatgtcga ctcgttccgt ctcggtgcgt ggccgcatgg cggctttggc gttgggctgg agcgcgtggt gatgctgtac cttgggctgg gcaacgtgcg ccttgcttcg ctgtttcccc gtgacccgca gcgcacgact ccgtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat