LebrA.00612.a

lysyl-tRNA synthetase, putative

CENTER ID: LebrA.00612.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.00612.a.B1.GE42519 full length 1 587
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.15.0260
UniProt: A4H7Z6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSSPKDLRIQ IEALAFQIAD IKKAGGTDCD EYKSLVKEMA ALRSRLPQDE KKAKKEKTIE PSYFESRLAM VKEMGLLGAA YPHKYHRQYT IPQYRRKYAP LIAEPGTSLD ETVTIAGRII KKRSSGSKLH FITIQGDMET VQVISAIANY TDESKFAEIH SKLKRGDIIG VAGKPSLSKS NEFSLNAVEV TLLSTCYHML PDDYFGLSSF EQRFRQRYLD LIVNRDNIKV FIQRANIIKY IRKFFDERDF VEVETPVLNQ IAGGAAARPF VTHHNDLNQT MFLRIAPELY LKELVVGGMD RVYELGKQFR NESIDLTHNP EFTSCEAYWA YMDYNDWMTA TEDLLYGLAM ELHGSPIVRY APKDSEGKLL PEVTFNFNKP FKRLHIIPEL EKRLKISFDN VDFESDAGIQ FLMDLCKKHK ADCPPPYTAP RLLDALIAEF LEPECHDPCF ICDHPRVMSP LAKWHRNDPR LTERFELFVN KKELANAYTE LNNPIVQREE FVKQMLNRDK GDDESMKIDE GFIAALEHAL PPTGGWGLGI DRLVMFLTSQ SNIKEVLLFP AMKPEGKNAI SYPPGTMLNG QGVPLLK
NT Sequence
atgtcgtccc ccaaagacct ccgtattcag atcgaggctc ttgccttcca aatcgcggac atcaagaagg cgggaggtac tgattgcgat gagtacaagt cactggtaaa ggaaatggct gcgcttcgca gccgacttcc gcaagatgag aagaaggcga agaaggaaaa gacaatagag ccgtcgtact ttgagtcgcg cctggcgatg gtcaaggaga tgggtcttct cggtgctgcc tacccgcata agtaccaccg tcagtatacg attcctcagt accgcaggaa gtacgcacct ttgatagcag agcccggtac ttcccttgac gaaacggtca ctatagcggg tcgcatcatt aagaagcgta gttccggatc aaagctgcac ttcattacta tccagggtga tatggagact gtgcaggtca tttccgccat tgctaattac actgacgagt cgaagtttgc tgagatccac tctaagctaa agcgtggtga tattatcggc gttgcgggaa agccatccct atcaaagagc aacgagttct cactcaatgc tgtcgaagtt acgcttctct caacttgcta ccacatgcta cccgacgact acttcggtct ttcttccttt gagcagcgct tccgtcagcg ctaccttgac ttgatcgtca atcgcgacaa tatcaaggta ttcattcagc gtgcaaatat catcaaatac attcgtaagt tctttgatga acgtgacttt gtggaggttg agacaccggt gctcaaccaa atcgctggtg gcgctgcggc tcgtcccttt gtgacgcacc ataacgatct caaccagacc atgttcctgc gtatagcacc agagctctac ttgaaagagc ttgtcgtcgg tggtatggat cgcgtgtacg aactcggcaa gcagttccgt aacgagagca ttgatctgac gcacaacccg gaattcacaa gctgcgaggc atactgggcg tatatggact acaacgattg gatgaccgcc acagaagacc ttttgtacgg cctggccatg gagcttcatg gctcacctat cgtccgctat gcgccaaagg attctgaagg aaagctgctg ccagaggtta cctttaattt caacaaaccg tttaaacgcc tgcacattat cccggagctg gagaagcgct tgaagatatc ctttgacaac gttgacttcg agtcggatgc gggtatccag tttctgatgg acttatgcaa gaagcacaag gccgactgcc cgccacccta cacagcacct cgtctcctcg atgccctcat cgccgagttt ctcgagcctg agtgccacga tccctgcttc atctgcgatc accctcgcgt gatgtcacct ctcgctaagt ggcaccgtaa tgacccccgc ctgacggagc gctttgagct gtttgtcaac aagaaagagc tcgctaatgc gtacaccgag ctgaacaacc ctatcgtgca acgtgaggag tttgtcaaac agatgctaaa ccgcgacaag ggcgatgacg agtcaatgaa gattgatgag ggcttcatcg ctgcgctgga gcatgcgctg ccgcccactg gtggctgggg tctcggtatt gaccgccttg tgatgttcct cacaagccag tccaacatca aggaagtgct tctgtttcct gccatgaagc ccgagggcaa gaatgcgatt agctaccctc cgggcacaat gcttaacggc cagggcgttc ctctgttgaa gtaa
Details for LebrA.00612.a.B1.GE42519
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS SPKDLRIQIE ALAFQIADIK KAGGTDCDEY KSLVKEMAAL RSRLPQDEKK AKKEKTIEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLI AEPGTSLDET VTIAGRIIKK RSSGSKLHFI TIQGDMETVQ VISAIANYTD ESKFAEIHSK LKRGDIIGVA GKPSLSKSNE FSLNAVEVTL LSTCYHMLPD DYFGLSSFEQ RFRQRYLDLI VNRDNIKVFI QRANIIKYIR KFFDERDFVE VETPVLNQIA GGAAARPFVT HHNDLNQTMF LRIAPELYLK ELVVGGMDRV YELGKQFRNE SIDLTHNPEF TSCEAYWAYM DYNDWMSATE DLLYGLAMEL HGSPIVRYAP KDSEGKLLPE VTFNFNKPFK RLHIIPELEK RLKISFDNVD FESDAGIQFL MDLCKKHKAD CPSPYTAPRL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFV KQMLNRDKGD DESMKIDEGF IAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTMLNGQG VPLLK
Validated NT Sequence
atggctcacc accaccacca ccatatgtcg tcccccaaag acctccgtat tcagatcgag gctcttgcct tccaaatcgc ggacatcaag aaggcgggag gtactgattg cgatgagtac aagtcactgg taaaggaaat ggctgcgctt cgcagccgac ttccgcaaga tgagaagaag gcgaagaagg aaaagacaat agagccgtcg tactttgagt cgcgcctggc gatggtcaag gagatgggtc ttctcggtgc tgcctacccg cataagtacc accgtcagta tacgattcct cagtaccgca ggaagtacgc acctttgata gcagagcccg gtacttccct tgacgaaacg gtcactatag cgggtcgcat cattaagaag cgtagttccg gatcaaagct gcacttcatt actatccagg gtgatatgga gactgtgcag gtcatttccg ctattgctaa ttacactgac gagtcgaagt ttgctgagat ccactctaag ctaaagcgtg gtgatattat cggcgttgcg ggaaagccat ccctatcaaa gagcaacgag ttctcactca atgctgtcga agttacgctt ctctcaactt gctaccacat gctacccgac gactacttcg gtctttcttc ctttgagcag cgcttccgtc agcgctacct tgacttgatc gtcaatcgcg acaatatcaa ggtattcatt cagcgtgcaa atatcatcaa atacattcgt aagttctttg atgaacgtga ctttgtggag gttgagacac cagtgctcaa ccaaatcgct ggtggcgctg cggctcgtcc ctttgtgacg caccataacg atctcaacca gaccatgttc ctgcgtatag caccagagct ctacttgaaa gagcttgtcg tcggtggtat ggatcgcgtg tacgaactcg gcaagcagtt ccgtaacgag agcattgatc tgacgcacaa cccggaattc acaagctgcg aagcatactg ggcgtatatg gactacaacg attggatgtc cgccacagaa gaccttttgt acggtctggc catggagctt catggctcac ctatcgtccg ctatgcgcca aaggattctg aaggaaagct gctgccagag gttaccttta atttcaacaa accgtttaaa cgcctgcaca ttatcccgga gctggagaag cgcttgaaga tatcctttga caacgttgac ttcgagtcgg atgcgggtat ccagtttctg atggacttat gcaagaagca caaggccgac tgcccgtcac cctacacagc acctcgtctc ctcgatgccc tcatcgccga gtttctcgag cctgagtgcc acgatccctg cttcatctgc gatcaccctc gcgtgatgtc acctctcgct aagtggcacc gtaatgaccc ccgcctgacg gagcgctttg agctgtttgt caacaagaaa gagctcgcta atgcgtacac cgagctgaac aaccctatcg tgcaacgtga ggagtttgtc aagcagatgc taaaccgcga caagggcgat gacgagtcaa tgaagattga tgagggcttc atcgctgcgc tggagcatgc gctgcctccc actggtggct ggggtctcgg tattgaccgc cttgtgatgt tcctcacaag ccagtccaac atcaaggaag tgcttctgtt tcctgccatg aagcccgagg gcaagaatgc gattagctac cctccaggca caatgcttaa cggncagggc gttcctctgt tgaag
Expected Protein Sequence
MAHHHHHHMS SPKDLRIQIE ALAFQIADIK KAGGTDCDEY KSLVKEMAAL RSRLPQDEKK AKKEKTIEPS YFESRLAMVK EMGLLGAAYP HKYHRQYTIP QYRRKYAPLI AEPGTSLDET VTIAGRIIKK RSSGSKLHFI TIQGDMETVQ VISAIANYTD ESKFAEIHSK LKRGDIIGVA GKPSLSKSNE FSLNAVEVTL LSTCYHMLPD DYFGLSSFEQ RFRQRYLDLI VNRDNIKVFI QRANIIKYIR KFFDERDFVE VETPVLNQIA GGAAARPFVT HHNDLNQTMF LRIAPELYLK ELVVGGMDRV YELGKQFRNE SIDLTHNPEF TSCEAYWAYM DYNDWMTATE DLLYGLAMEL HGSPIVRYAP KDSEGKLLPE VTFNFNKPFK RLHIIPELEK RLKISFDNVD FESDAGIQFL MDLCKKHKAD CPPPYTAPRL LDALIAEFLE PECHDPCFIC DHPRVMSPLA KWHRNDPRLT ERFELFVNKK ELANAYTELN NPIVQREEFV KQMLNRDKGD DESMKIDEGF IAALEHALPP TGGWGLGIDR LVMFLTSQSN IKEVLLFPAM KPEGKNAISY PPGTMLNGQG VPLLK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgt cccccaaaga cctccgtatt cagatcgagg ctcttgcctt ccaaatcgcg gacatcaaga aggcgggagg tactgattgc gatgagtaca agtcactggt aaaggaaatg gctgcgcttc gcagccgact tccgcaagat gagaagaagg cgaagaagga aaagacaata gagccgtcgt actttgagtc gcgcctggcg atggtcaagg agatgggtct tctcggtgct gcctacccgc ataagtacca ccgtcagtat acgattcctc agtaccgcag gaagtacgca cctttgatag cagagcccgg tacttccctt gacgaaacgg tcactatagc gggtcgcatc attaagaagc gtagttccgg atcaaagctg cacttcatta ctatccaggg tgatatggag actgtgcagg tcatttccgc cattgctaat tacactgacg agtcgaagtt tgctgagatc cactctaagc taaagcgtgg tgatattatc ggcgttgcgg gaaagccatc cctatcaaag agcaacgagt tctcactcaa tgctgtcgaa gttacgcttc tctcaacttg ctaccacatg ctacccgacg actacttcgg tctttcttcc tttgagcagc gcttccgtca gcgctacctt gacttgatcg tcaatcgcga caatatcaag gtattcattc agcgtgcaaa tatcatcaaa tacattcgta agttctttga tgaacgtgac tttgtggagg ttgagacacc ggtgctcaac caaatcgctg gtggcgctgc ggctcgtccc tttgtgacgc accataacga tctcaaccag accatgttcc tgcgtatagc accagagctc tacttgaaag agcttgtcgt cggtggtatg gatcgcgtgt acgaactcgg caagcagttc cgtaacgaga gcattgatct gacgcacaac ccggaattca caagctgcga ggcatactgg gcgtatatgg actacaacga ttggatgacc gccacagaag accttttgta cggcctggcc atggagcttc atggctcacc tatcgtccgc tatgcgccaa aggattctga aggaaagctg ctgccagagg ttacctttaa tttcaacaaa ccgtttaaac gcctgcacat tatcccggag ctggagaagc gcttgaagat atcctttgac aacgttgact tcgagtcgga tgcgggtatc cagtttctga tggacttatg caagaagcac aaggccgact gcccgccacc ctacacagca cctcgtctcc tcgatgccct catcgccgag tttctcgagc ctgagtgcca cgatccctgc ttcatctgcg atcaccctcg cgtgatgtca cctctcgcta agtggcaccg taatgacccc cgcctgacgg agcgctttga gctgtttgtc aacaagaaag agctcgctaa tgcgtacacc gagctgaaca accctatcgt gcaacgtgag gagtttgtca aacagatgct aaaccgcgac aagggcgatg acgagtcaat gaagattgat gagggcttca tcgctgcgct ggagcatgcg ctgccgccca ctggtggctg gggtctcggt attgaccgcc ttgtgatgtt cctcacaagc cagtccaaca tcaaggaagt gcttctgttt cctgccatga agcccgaggg caagaatgcg attagctacc ctccgggcac aatgcttaac ggccagggcg ttcctctgtt gaagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat