SSGCID: Target Page

LebrA.00600.b

tryptophanyl-tRNA synthetase, putative

CENTER ID:	LebrA.00600.b
ORGANISM:	Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
LebrA.00600.b.B2.GE42518	soluble domain		17	489

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	TriTrypDB:LbrM.23.0330
UniProt:	A4HCP4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRRVTCWAWT LRAGVCTFTA LRSTSERSDC AKDPRELAQH PPGGGDADTE DVITPWTVVA KGPQGINYDR VLTTFKAERV DDSARQHIRD AIVKCHERTA SVTSVNAQNT PTPVSTAVSP GGVPAQVSVS PAAQQQQPPP PPLHHFFSRG IAFSHRDLHK ALDDIEASIK PDEPSVFLYT GRGPSAGTMH AGHVLPFMLA KYLQDVFNLP LVIQITDDEK FLFRGVPFEG AVAEEVILNN IKDIIAFGFH PRHTFIFRNT HYMGELYPTV LPLQRSMTGN SVKHTLGITD SDNVGKFAFP ATQAAPCFST AFRRVLPNGD RPMRCLIPCA IDQDPFFVLT RAAAPRLKQP PPALLHTQFL PALKGLEHKM SSSAEENGVI TLHDTDKQVR KKLRRAFSGG CATLEQMQET GANLKVDVAY QYLRFFCRDD ALFADVTQRY GRGTMNSGDV KDLAADCIIR DVLHDWREQR AKVSDDDVVN FCRIRDILH

NT Sequence

atgcgtcgcg tcacctgctg ggcatggact ttgcgcgccg gcgtgtgcac tttcacagca ctgcgtagca caagcgagag atctgactgc gcgaaggatc ctcgcgaact tgcccaacac ccccccggag gcggtgatgc cgatacggaa gacgtcatta ccccctggac ggtagtcgcc aagggcccgc aggggatcaa ctatgatcgc gtgttaacca cattcaaagc ggagcgagtg gacgacagcg cacgtcagca tattcgtgat gccatcgtca agtgccacga gcgaaccgca tccgtcacct ctgtcaacgc ccagaacacc ccgacccccg tttcgactgc tgtgtcacct ggaggtgtgc cagcgcaggt ctccgtgtct cctgccgcgc agcagcagca accaccacca ccgccgcttc accacttctt cagccgcggc attgccttct cgcaccgcga cttgcacaag gcactcgacg acatcgaggc ttccatcaag ccggatgagc cttccgtctt cctctacacc ggccgtggcc ccagcgctgg caccatgcac gcgggccatg tgctgccctt catgctggcc aagtacctcc aagacgtctt caatctccct ctcgtgatac aaatcaccga cgacgaaaag tttctctttc gcggcgtccc cttcgagggg gccgtggcgg aggaggtcat tctcaacaac atcaaggata tcatcgcctt tggcttccac ccgcgccata ccttcatctt tcgcaacacg cactacatgg gcgagctgta cccgacagtg ctgccgctcc agcgtagcat gacaggcaac tcagtgaagc acactctcgg catcaccgac agcgacaacg tcggcaaatt cgcctttcct gcaacccagg cagctccgtg cttcagcacc gcttttcgtc gcgtcctgcc gaacggtgac agaccgatgc ggtgcctgat tccctgcgcc attgaccagg accctttctt tgtgctcacc cgcgctgccg cgccgcggct gaagcagccg ccaccggccc ttcttcacac tcagttcctg cccgcactca aggggctgga gcacaagatg agcagcagtg cagaggagaa cggcgtcatt acgctacacg acacggataa gcaggtgcgc aagaagctgc gccgtgcctt ctcgggtggc tgcgccaccc tagagcagat gcaggagacg ggggcgaacc tgaaggtgga tgtggcctac cagtacctgc gcttcttctg ccgtgacgac gcactcttcg ccgacgtgac gcagcgctac ggcagaggta cgatgaacag cggggatgtg aaggacctgg cggcggactg catcatccgt gacgtgctgc acgactggcg ggaacagcgc gcaaaggtaa gcgacgatga cgtggtaaac ttctgcagga ttcgagacat tcttcactga

Details for LebrA.00600.b.B2.GE42518

HARVESTED ON:	7/3/2018
SEQUENCED ON:	7/10/2018
EXPECTED MW:	53kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHMTFT ALRSTSERSD CATDPRELAQ HPPGGGDADT EDVITPWTVV AKGPQGINYD RVLTTFKAER VDDSARQHIR DAIAKCHERT ASVTSVNAQN TPTPVSIAVS PGGVPAQVCV SPAAQQQQPP PPPLHHFFSR GIAFSHRDLH KALDDIEASI KPEEPSVFLY TGRGPSAGTM HAGHVLPFML AKYLQDVFNL PLVIQITDDE KFLFRGVPFE GAVAEEVILN NIKDIIAFGF HPRHTFIFRN THYMGELYPT VLPLQRSMTG NSVKHTLGLT DSDNVGKFAF PATQAAPCFS TAFRRVLPNG DRPMRCLIPC AIDQDPFFVL TRAAAPRLKQ PPPALLHTQF LPALKGLEHK MSSSAEENGV ITLHDTDKQV RKKLRRAFSG GCATLEQMQE TGANLKVDVA YQYLRFFCRD DALFADVTQR YGRGTMNSGD VKDLAADCII CDVLHDWREQ REKVSDDDVV NF

Validated NT Sequence

atggctcacc accaccatat gactttcaca gcactgcgta gcacaagcga gagatctgac tgcgcgacgg atcctcgcga acttgcccaa cacccccccg gaggcggtga tgccgatacg gaagacgtca ttaccccctg gacggtagtc gccaagggcc cgcaggggat caactatgat cgcgtgttaa ccacattcaa agcagagcga gtggacgaca gcgcacgtca gcatattcgt gatgccatcg ccaagtgcca cgaacgaacc gcatccgtca cctctgtcaa cgcccagaac accccgaccc ccgtttcgat tgctgtgtca cctggaggtg tgccagcgca ggtctgcgtg tctcctgccg cgcagcagca gcaaccacca ccaccgccgc ttcaccactt cttcagccgc ggcattgcct tctcgcaccg cgatttgcac aaggcactcg acgacatcga ggcttccatc aagccggagg agccttccgt cttcctctac accggccgtg gccccagcgc tggcaccatg cacgcgggcc atgtgctgcc cttcatgctg gccaagtacc tccaagacgt cttcaatctc cctctcgtga tacaaatcac cgacgacgaa aagtttctct ttcgcggcgt ccccttcgag ggggccgtgg cggaggaggt cattctcaac aacatcaagg atatcatcgc ctttggcttc cacccgcgcc ataccttcat ctttcgcaac acgcactaca tgggcgagct gtacccgaca gtgctgccgc tccagcgtag catgacaggc aactcagtga agcacactct cggcctcacc gacagcgaca acgtcggcaa attcgccttt cctgcaaccc aggcagctcc gtgcttcagc accgcttttc gtcgcgtcct gccgaacggt gacagaccga tgcggtgcct gattccctgc gccattgacc aggacccttt ctttgtgctc acccgcgctg ccgcgccgcg gctgaagcag ccgccaccgg cccttcttca cactcagttc ctgcccgcac tcaaggggtt ggagcacaag atgagcagca gtgcagagga gaacggcgtc attacgctac acgacacgga taagcaggtg cgcaagaagc tgcgccgcgc tttctcgggt ggctgcgcca ccctagagca gatgcaggag acgggggcga acctgaaggt ggatgtggcc taccagtacc tgcgcttctt ctgccgtgac gacgcactct tcgccgacgt gacacagcgc tacggcagag gtacgatgaa cagcggggat gtgaaggacc tggcggcgga ctgcatcatc tgtgacgttc tgcacgactg gcgggaacag cgcgaaaagg taagcgacga tgacgtggta aacttct

Expected Protein Sequence

MAHHHHHHTF TALRSTSERS DCAKDPRELA QHPPGGGDAD TEDVITPWTV VAKGPQGINY DRVLTTFKAE RVDDSARQHI RDAIVKCHER TASVTSVNAQ NTPTPVSTAV SPGGVPAQVS VSPAAQQQQP PPPPLHHFFS RGIAFSHRDL HKALDDIEAS IKPDEPSVFL YTGRGPSAGT MHAGHVLPFM LAKYLQDVFN LPLVIQITDD EKFLFRGVPF EGAVAEEVIL NNIKDIIAFG FHPRHTFIFR NTHYMGELYP TVLPLQRSMT GNSVKHTLGI TDSDNVGKFA FPATQAAPCF STAFRRVLPN GDRPMRCLIP CAIDQDPFFV LTRAAAPRLK QPPPALLHTQ FLPALKGLEH KMSSSAEENG VITLHDTDKQ VRKKLRRAFS GGCATLEQMQ ETGANLKVDV AYQYLRFFCR DDALFADVTQ RYGRGTMNSG DVKDLAADCI IRDVLHDWRE QRAKVSDDDV VNFCRIRDIL H

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catactttca cagcactgcg tagcacaagc gagagatctg actgcgcgaa ggatcctcgc gaacttgccc aacacccccc cggaggcggt gatgccgata cggaagacgt cattaccccc tggacggtag tcgccaaggg cccgcagggg atcaactatg atcgcgtgtt aaccacattc aaagcggagc gagtggacga cagcgcacgt cagcatattc gtgatgccat cgtcaagtgc cacgagcgaa ccgcatccgt cacctctgtc aacgcccaga acaccccgac ccccgtttcg actgctgtgt cacctggagg tgtgccagcg caggtctccg tgtctcctgc cgcgcagcag cagcaaccac caccaccgcc gcttcaccac ttcttcagcc gcggcattgc cttctcgcac cgcgacttgc acaaggcact cgacgacatc gaggcttcca tcaagccgga tgagccttcc gtcttcctct acaccggccg tggccccagc gctggcacca tgcacgcggg ccatgtgctg cccttcatgc tggccaagta cctccaagac gtcttcaatc tccctctcgt gatacaaatc accgacgacg aaaagtttct ctttcgcggc gtccccttcg agggggccgt ggcggaggag gtcattctca acaacatcaa ggatatcatc gcctttggct tccacccgcg ccataccttc atctttcgca acacgcacta catgggcgag ctgtacccga cagtgctgcc gctccagcgt agcatgacag gcaactcagt gaagcacact ctcggcatca ccgacagcga caacgtcggc aaattcgcct ttcctgcaac ccaggcagct ccgtgcttca gcaccgcttt tcgtcgcgtc ctgccgaacg gtgacagacc gatgcggtgc ctgattccct gcgccattga ccaggaccct ttctttgtgc tcacccgcgc tgccgcgccg cggctgaagc agccgccacc ggcccttctt cacactcagt tcctgcccgc actcaagggg ctggagcaca agatgagcag cagtgcagag gagaacggcg tcattacgct acacgacacg gataagcagg tgcgcaagaa gctgcgccgt gccttctcgg gtggctgcgc caccctagag cagatgcagg agacgggggc gaacctgaag gtggatgtgg cctaccagta cctgcgcttc ttctgccgtg acgacgcact cttcgccgac gtgacgcagc gctacggcag aggtacgatg aacagcgggg atgtgaagga cctggcggcg gactgcatca tccgtgacgt gctgcacgac tggcgggaac agcgcgcaaa ggtaagcgac gatgacgtgg taaacttctg caggattcga gacattcttc actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat