LebrA.00433.a

Protein kinase domain-containing protein

CENTER ID: LebrA.00433.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.00433.a.HZ2.GE43703 kinase domain 1 337
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.27.0110
UniProt: A4HFC9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKYEILAQI GDGTFGSVAK AVSKKTGQLV AIKKMKQKFY TWEECVKLPE VDVVRRIHGH PNVVKLREVI RENNELFFVF EYMDGDLLSV IKKAKQQVGP SASRPSAAPL IPYPLVKNYM RQILQALVYI HKRGYFHRDM KPENLLIRKE TSGDDVLKLA DFGLVKEIRS RPPFTDYVST RWYRAPELLL QDRYYGAAVD VWAAGCIMAE LITMRPLFAG TNEVDQLFKI MSVLGSPTEE VWAGGLRLAK KIRYTFPAVA GSGLAQVLPS HIPLPALDLL RQMLVYDPKV RLTAEQCLQH PFFKVSIDEH NAPSSAVLDQ LALVAKRMLP GSKTAPPALK SPTVDQLAAL KVARASTDAS LSATSPRKFY QLSTLKDSAP SFPKVPVPLS TSPPSLDALS NAPSPLRGLG TLSPEGLPAL ANGYPRPSLT TASKGKRLLQ ETAGTSLAKK LAGLKNIKAG NGKLSVPYTL AAASAAHSPS AAPNLMVSAP KARPSTLVPI TNNRDTTTPQ WRRNPHKEAE TTSPTATSAG AKREVNLDEL MDEFASEMTA MGVLAQRYDA GASGELNNKS CTSQSALTHN PVATLLNNSR YPRKSIANSN PPFKASNLPR TTRPLDTRKM DDGVARSDLP RTDHSSPKGV STSLKALLAK HKMSGLAFQ
NT Sequence
atggacaagt acgaaatcct cgctcaaatt ggcgacggca ccttcgggtc cgttgcaaag gctgtgagca agaaaactgg ccagcttgta gccatcaaga agatgaagca gaaattctac acgtgggagg agtgcgtcaa gcttcccgag gtagacgttg tgcggcgcat tcacggccac cccaacgtgg tgaagctgcg tgaggtaatt cgcgagaaca acgaactgtt cttcgtcttc gagtacatgg acggagacct cctcagcgtc atcaagaaag caaagcaaca ggtcggaccg tccgccagta gaccgtctgc cgcgccgtta attccgtatc cgcttgtgaa gaactacatg cgccaaattc tgcaggcact tgtgtacatc cacaagcgcg gctacttcca tcgcgacatg aagcctgaaa accttctcat tcggaaagag acaagcggcg acgatgtgct gaagctggca gatttcggac ttgtcaagga gatccgttcc cgcccgccgt tcaccgacta tgtgtcgacg cggtggtacc gcgcgccaga gctgctgctg caggatcgct actacggcgc tgcggtggat gtatgggctg ccggctgcat tatggcggag ctcatcacca tgcggcctct ttttgcaggc accaacgagg tagatcagct tttcaagata atgtcggtgc ttgggtctcc cactgaggag gtgtgggctg gtgggctgcg cttggccaaa aagatccgct atacgtttcc agcagtagcc ggttccggtc ttgctcaagt gctgccgtcg catatcccgt taccggcgct ggatctgctg cgtcagatgc ttgtctacga cccgaaggtg aggctgacgg cggagcaatg cctgcagcat ccctttttca aagtcagcat tgacgagcac aacgcgccat cctccgccgt gctggatcag ctggcgctcg tggcaaagcg gatgctacca gggtcgaaaa cagcgccacc ggcgctgaag agccccaccg tagatcaatt ggcggcgctg aaggtggcca gagcctccac cgacgcatca ctctcggcta cctctccacg taagttttac caactcagca ccctcaagga ctccgccccg tcgttcccaa aggtcccggt ccccttgtcc acctccccac cgtcccttga cgcgctgtcg aacgctccat cacctctgcg tgggcttggg accctctcac cagaggggct accagcactc gccaacggct acccgcgccc cagcctgacg acggcgtcca agggaaaacg cctcttacag gaaacagcag gcacttcgct tgcaaagaag ctagcgggct tgaaaaacat caaggctgga aacgggaagc tctctgttcc ttacacgctg gcggctgcca gcgctgcgca ctcgcccagt gcagcaccta atttgatggt ctccgcgccc aaggcgaggc catccaccct ggtacccatc accaacaacc gcgacaccac gacgccgcag tggcgccgca acccgcacaa ggaagctgag actacttctc ctacggcaac gtctgcaggg gcgaagcggg aagtgaacct ggacgagcta atggatgagt ttgcttcaga gatgaccgcc atgggagtgc tagcgcagcg atacgacgcg ggtgcctcag gcgaactgaa caacaaaagc tgcacttctc agtcagcgct tacgcacaat cctgtggcca cgctgctgaa caacagccgg tacccaagaa agtccatcgc gaactctaac cctccgttta aggcaagcaa cttgcctcgc accacacgcc cactagatac aaggaagatg gacgatggtg tcgcgaggag cgaccttcct cgcacagatc actcatcccc aaagggggtc tcgacatcgc tgaaggcgct cctcgcaaag cacaagatga gtggtctggc gtttcaatag
Details for LebrA.00433.a.HZ2.GE43703
HARVESTED ON: 7/6/2020
SEQUENCED ON: 6/23/2020
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 87
PERCENT COVERAGE: 49
Validated AA Sequence
MAHHHHHHHH MKSDSEVNQE AKPEVKPEVK PETHINLKVS DGSSEIFFKI KKTTPLRRLM EAFAKRQGKE MDSLRFLYDG IRIQADQTPE DLDMEDNDII EAHREQIGGT LEAQTQGPGS MDKYEILAQI GDGTFGSVAK AVXKKTGQLV AXKKXKQKFY XWXEXVKLPX XXVVRXIXGH PXVVKLREVI RENNELXFXF EYMXXXPPXR HXRKXKQQVG PSRQ
Validated NT Sequence
ttntnacggn ggcgctgttt tcgaccctgg tagcatccgc tttgccacga gcgccagctg atccagcacg gcggaggatg gcgcgttgtg ctcgtcaatg ctgactttga aaaagggatg ctgcaggcat tgctccgccg tcagcctcac cttcgggtcg tagacaagca tctgacgcag cagatccagc gccggcaacg ggatatgcga cggcagcact tgagcaagac cggaaccggc tactgctgga aacgtatagc ggatcttttt ggccaagcgc agnccnccng cccacacctc nnagtgggag acccaagcac cgacattatc ttgaaaagct gatctacctc gttggtgcct gcaaaaagag gccgcatggt gatgagctcc gccatantgc agccggcagc ccacacatcc accgcagcgc cgtantagcg atcnngnagc ancagctctg gcgcgcggta ncnccgcgtc nacacntnnn nngtgaacng cgggcgggaa cggatctcct tgacaagtcc naaatctgcc agcttcannn nnnnnnngcn nnntgtctct tnnnnnatga naaggttttc aggcttcatg tcgcgatgga agtanccgcg cttngtggat gnncnnnant gccngnnnaa tttggcgcat gtanttncnt tcacnnncgg anacggannt aacggcgcgg cagacggtct actggcggga cggcccgacc tgttgcttnt gctttcttng atgacgntga ggaggtntcc nntccatgta ctcgaagang aagancagtt cgttgttctc gcgaattacc tcacgcagct tcaccacgtn ggggtgnccg ngaatgcncc gcacaacgtn tncntcggga agcttgacnc actcntccca cgngtagaat ttctgcttcn tcttcttgan ggctacaagc tggccagttt tcttgntcac agcctttgca acggacccga aggtgccgtc gccaatttga gcgaggattt cgtacttgtc catcgaacca ggaccctggg tctgagcttc cagggtacca ccaatctgtt ctctgtgagc ctcaataata tcgttatcct ccatgtccaa atcttcaggg gtctgatcag cttgaattct aataccgtcg tacaagaatc ttaaggagtc catttcctta ccctgtcttt tagcgaacgc ttccatcagc cttcttaaag gagtggtctt tttgatcttg aagaagatct ctgaagatcc atcggacacc tttaaattga tgtgagtctc aggcttgact tctggcttga cctctggctt agcttcttga ttgacttctg agtccgactt catatgatgg tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMK SDSEVNQEAK PEVKPEVKPE THINLKVSDG SSEIFFKIKK TTPLRRLMEA FAKRQGKEMD SLRFLYDGIR IQADQTPEDL DMEDNDIIEA HREQIGGTLE AQTQGPGSMD KYEILAQIGD GTFGSVAKAV SKKTGQLVAI KKMKQKFYTW EECVKLPEVD VVRRIHGHPN VVKLREVIRE NNELFFVFEY MDGDLLSVIK KAKQQVGPSA SRPSAAPLIP YPLVKNYMRQ ILQALVYIHK RGYFHRDMKP ENLLIRKETS GDDVLKLADF GLVKEIRSRP PFTDYVSTRW YRAPELLLQD RYYGAAVDVW AAGCIMAELI TMRPLFAGTN EVDQLFKIMS VLGSPTEEVW AGGLRLAKKI RYTFPAVAGS GLAQVLPSHI PLPALDLLRQ MLVYDPKVRL TAEQCLQHPF FKVSIDEHNA PSSAVLDQLA LVAKRMLPGS KTAPP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgaagtcgga ctcagaagtc aatcaagaag ctaagccaga ggtcaagcca gaagtcaagc ctgagactca catcaattta aaggtgtccg atggatcttc agagatcttc ttcaagatca aaaagaccac tcctttaaga aggctgatgg aagcgttcgc taaaagacag ggtaaggaaa tggactcctt aagattcttg tacgacggta ttagaattca agctgatcag acccctgaag atttggacat ggaggataac gatattattg aggctcacag agaacagatt ggtggtaccc tggaagctca gacccagggt cctggttcga tggacaagta cgaaatcctc gctcaaattg gcgacggcac cttcgggtcc gttgcaaagg ctgtgagcaa gaaaactggc cagcttgtag ccatcaagaa gatgaagcag aaattctaca cgtgggagga gtgcgtcaag cttcccgagg tagacgttgt gcggcgcatt cacggccacc ccaacgtggt gaagctgcgt gaggtaattc gcgagaacaa cgaactgttc ttcgtcttcg agtacatgga cggagacctc ctcagcgtca tcaagaaagc aaagcaacag gtcggaccgt ccgccagtag accgtctgcc gcgccgttaa ttccgtatcc gcttgtgaag aactacatgc gccaaattct gcaggcactt gtgtacatcc acaagcgcgg ctacttccat cgcgacatga agcctgaaaa ccttctcatt cggaaagaga caagcggcga cgatgtgctg aagctggcag atttcggact tgtcaaggag atccgttccc gcccgccgtt caccgactat gtgtcgacgc ggtggtaccg cgcgccagag ctgctgctgc aggatcgcta ctacggcgct gcggtggatg tatgggctgc cggctgcatt atggcggagc tcatcaccat gcggcctctt tttgcaggca ccaacgaggt agatcagctt ttcaagataa tgtcggtgct tgggtctccc actgaggagg tgtgggctgg tgggctgcgc ttggccaaaa agatccgcta tacgtttcca gcagtagccg gttccggtct tgctcaagtg ctgccgtcgc atatcccgtt accggcgctg gatctgctgc gtcagatgct tgtctacgac ccgaaggtga ggctgacggc ggagcaatgc ctgcagcatc cctttttcaa agtcagcatt gacgagcaca acgcgccatc ctccgccgtg ctggatcagc tggcgctcgt ggcaaagcgg atgctaccag ggtcgaaaac agcgccaccg taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa