LebrA.00424.a

AGC family serine/threonine kinase, putative

CENTER ID: LebrA.00424.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.00424.a.B2.GE40013 PK-like domain 46 335
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TritrypDB:LbrM.25.1930
RefSeq: XP_001565695.1
UniProt: A4HED7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFMKLFKKKD KKDKEEEKKP ELTPPVEEKS GSTPEPPQEK LTKDDFETLD VLGKGSFAYV VLCRRLSTNK YYAMKVINKQ GLLDHKRVQD VFTERNVLTR LNHPYLLKLY WTFQSEHKLF FVMDYMPGGD LDKYMNTVPK KQLDATTAQL YGAEILLAIM CLHEHSVIYR DLKPENILLD AEGHCALADF GLSKDFHKDG QDVSDKDLRA NSFVGSPFYV APDVLRQREY TSAVDFWSFG ILLYRMLCGR TPFNGRSMTE VFDNILYSDL SFPSGVTIPP EAKDLISRLL MKDPNRRIKG PEVKLHPYWN GINFEEVMQR KIPPPRWTPL QSVEEMLAAR KNVVAGTSAS LKNPHQVVNT PAQSSQLNAG QQQLFGGFSC TADSHLHNS
NT Sequence
atgtttatga agttgttcaa gaaaaaggat aaaaaggaca aggaggagga gaagaagccc gagttaacac ctcccgtgga ggaaaagagc ggaagcaccc ctgagccgcc gcaggagaag ctgaccaaag acgacttcga gaccctcgac gtgctcggaa agggctcctt cgcctatgtg gtgctgtgcc gtcgcctgtc cacgaacaag tactacgcca tgaaggtgat caacaagcag ggcttgctgg accacaagcg cgttcaggat gtctttacgg agcgcaacgt gctgactcgc ctgaaccacc cctacctact gaagctgtac tggacttttc agtccgagca caagctgttc ttcgtcatgg actacatgcc tggcggtgac ctcgacaagt acatgaacac cgtccccaaa aagcagcttg atgcaacgac ggcgcagctg tacggcgccg agattctgtt ggccatcatg tgcctgcacg agcacagcgt tatctatcga gacctgaagc cagagaacat tctgctggat gctgagggtc actgcgctct tgcagacttc ggcctgtcta aggatttcca caaggacggt caagacgtgt cggacaagga ccttcgcgcg aactcgttcg ttgggtcgcc gttctacgtg gcgcctgatg tactgcgcca acgcgagtac accagcgcgg tggacttctg gtctttcggc attctcctgt accgcatgct ctgcggccgc actcccttca acgggcgcag catgacagag gtgttcgaca acatcttgta ctcagacctc tctttcccca gtggcgtgac gataccgccg gaggccaagg acctgatcag ccgcttgctg atgaaggacc ccaaccgccg catcaaaggt cctgaagtga agctgcaccc gtactggaac gggatcaact ttgaggaggt gatgcaacgc aagattccac cgccacgctg gaccccgctc cagtcagtgg aggagatgct ggctgcccgc aagaacgtcg tcgctggaac ctcggcctct ttgaagaatc ctcaccaggt cgtaaatacg ccagcgcaga gctcacagct caacgcaggt cagcagcagc tgtttggcgg cttctcctgc acggcggaca gtcacctgca caacagctga
Details for LebrA.00424.a.B2.GE40013
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMF ETLDVLGKGS FAYVVLCRRL STNKYYAMKV INKQGLLDHK RVQDVFTERN VLTRLNHPYL LKLYWTFQSE HKLFFVMDYM PGGDLDKYMN TVPKKQLDAT TAQLYGAEIL LAIMCLHEHS VIYRDLKPEN ILLDAEGHCA LADFGLSKDF HKDGQDVSDK DLRANSFVGS PFYVAPDVLR QREYTSAVDF WSFGILLYRM LCGRTPFNGR SMTEVFDNIL YSDLSFPSGV TVPPEAKDLI SRLLMKDPNR RIKGPEVKLH PYWNGINFEE VMQRKIPPPR WTPLQSVEE
Validated NT Sequence
atcttactca cttactcctc cactgactgg agcggggtcc accgtggcgg tggaatcttg cgttgcatca cctcctcaaa gttgatcccg ttccagtacg ggtgcagctt cacttcagga cctttgatgc ggcggttggg gtccttcatc agcaagcggc tgatcaggtc cttggcctcc ggcggtaccg tcacgccact ggggaaagag aggtctgagt acaagatgtt gtcgaacacc tctgtcatgc tgcgcccgtt gaagggagtg cggccgcaca gcatgcggta caggagaatg ccgaaagacc agaagtccac cgcgctggtg tactcgcgtt ggcgcagtac atcaggcgcc acgtagaacg gcgacccaac gaacgagttc gcgcgaaggt ccttgtccga cacgtcttga ccgtccttgt ggaaatcctt ggacaggccg aagtctgcaa gagcgcagtg accctcagca tccagcagaa tgttctctgg cttcaggtct cgatagataa cgctgtgctc gtgcaggcac atgatggcca acagaatctc ggcgccgtac agctgcgccg tcgttgcatc aagctgcttc ttggggacgg tgttcatgta cttgtcgagg tcaccgccag gcatgtagtc catgacgaag aacagcttgt gctcggactg aaaagtccag tacagcttca gcaggtaagg gtggttcagg cgagtcagca cgttgcgctc cgtaaagaca tcctgaacgc gcttgtggtc cagcaagccc tgcttgttga tcaccttcat ggcgtagtac ttgtttgtgg acaggcgacg gcacagcacc acataggcga aggagccctt tccgagcacg tcgagggtct cgaacatatg gtggtggtgg tggtgagcca t
Expected Protein Sequence
MAHHHHHHFE TLDVLGKGSF AYVVLCRRLS TNKYYAMKVI NKQGLLDHKR VQDVFTERNV LTRLNHPYLL KLYWTFQSEH KLFFVMDYMP GGDLDKYMNT VPKKQLDATT AQLYGAEILL AIMCLHEHSV IYRDLKPENI LLDAEGHCAL ADFGLSKDFH KDGQDVSDKD LRANSFVGSP FYVAPDVLRQ REYTSAVDFW SFGILLYRML CGRTPFNGRS MTEVFDNILY SDLSFPSGVT IPPEAKDLIS RLLMKDPNRR IKGPEVKLHP YWNGINFEEV MQRKIPPPRW TPLQSVEE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttcgaga ccctcgacgt gctcggaaag ggctccttcg cctatgtggt gctgtgccgt cgcctgtcca cgaacaagta ctacgccatg aaggtgatca acaagcaggg cttgctggac cacaagcgcg ttcaggatgt ctttacggag cgcaacgtgc tgactcgcct gaaccacccc tacctactga agctgtactg gacttttcag tccgagcaca agctgttctt cgtcatggac tacatgcctg gcggtgacct cgacaagtac atgaacaccg tccccaaaaa gcagcttgat gcaacgacgg cgcagctgta cggcgccgag attctgttgg ccatcatgtg cctgcacgag cacagcgtta tctatcgaga cctgaagcca gagaacattc tgctggatgc tgagggtcac tgcgctcttg cagacttcgg cctgtctaag gatttccaca aggacggtca agacgtgtcg gacaaggacc ttcgcgcgaa ctcgttcgtt gggtcgccgt tctacgtggc gcctgatgta ctgcgccaac gcgagtacac cagcgcggtg gacttctggt ctttcggcat tctcctgtac cgcatgctct gcggccgcac tcccttcaac gggcgcagca tgacagaggt gttcgacaac atcttgtact cagacctctc tttccccagt ggcgtgacga taccgccgga ggccaaggac ctgatcagcc gcttgctgat gaaggacccc aaccgccgca tcaaaggtcc tgaagtgaag ctgcacccgt actggaacgg gatcaacttt gaggaggtga tgcaacgcaa gattccaccg ccacgctgga ccccgctcca gtcagtggag gagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat