LebrA.00164.a

arginyl-tRNA synthetase, putative

CENTER ID: LebrA.00164.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.00164.a.B1.GE42517 full length 1 692
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.00164.a.B1.PW38604 full length 1 692

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.27.1450
UniProt: A4HFQ8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRTAAATVNV ELALKEIVQA TITKAFPLVP AQEPLITLGK VSEYQCNNAM GLVKVLSQQP QSIKMSPQMV GEELKKNLVE NDIIDEFEPT TKGFINISIR KEWVAQMVNE VLNQGIRPPV VKKQRVLVDF SSPNIAKEMH VGHLRSTIIG ESISRLLEFC LHDVSRINHI GDWGTAFGML ILYIKRKFPD YTTNPPAISD LTAFYRAAKK CFDEDQQFKE QARLEVVKLQ ALEEDSIQAW KYICDVSREE FNGVYARLGA TITERGESFY NPLIPKVLNL LEEADQIEVS DGAKLIISRE ERKLDSLDSR DMTKLASQHL AFLSRDGSSY HPNLIAAMKK VGILTGAEGE ESVVLSKKEV KLWKKFDIRM DLDKLMAQLA PLYKKQLDPL FLEAFKAAGI VKGDCILVPR FSFPLMVVKS DGGYTYDTTD VTATYHRFVV EKMDRVIYCT DVGQYEHFRM CLQTAKDMGW MEHATWDHAG FGLVTGADGK KIKTRSGETA KLKDLMDEAV ERSLAILKER EAGERSQGHS EEEMQTLSNI IGIGAIKYFD LKQTRTSDYA FSYDKMLDMS GNTAVFLLYQ YARICSIKRK AGVAEEELLK ITDISLETPQ EKNLALCALR FQTVILKAVE DLFPHHLADF AYELMTNFST FFQHCRVVDD PLQNSRLCLI ELTRITLKKT LELLAIETAE RL
NT Sequence
atgcgcaccg ctgccgctac cgtgaatgtg gagctggcgc tgaaggagat tgtccaggcg accattacca aggccttccc gctcgtcccg gcccaggagc ctctcatcac ccttggtaag gttagcgagt atcagtgcaa caatgccatg ggactggtga aggtgctctc tcagcagcct cagtcaataa aaatgagccc gcagatggtc ggcgaggagc tcaagaagaa cttggtggag aacgacataa tcgacgagtt cgaacccacg accaagggct tcatcaatat cagcatccgc aaggaatggg tggctcagat ggtgaacgag gtgctgaacc agggcattcg tccacctgtc gtgaagaagc agcgcgtgct cgtcgatttt tcgtcgccga acatcgccaa ggagatgcac gtcggacatc tccgctctac tatcatcggt gagtcgatca gccgtctcct ggagttctgt ctgcacgacg tttcacgcat aaaccacatc ggtgactggg gcacggcgtt cggcatgctc attctgtaca tcaagcgcaa gttccccgac tacaccacga acccgccggc catctccgac ctcaccgcct tctaccgcgc ggcgaagaag tgcttcgacg aggaccagca gttcaaggag caggcccgcc tcgaggtggt gaagctgcag gcgctggagg aggactctat ccaagcgtgg aagtacatct gcgacgtgtc gcgcgaggag ttcaacgggg tctacgcccg cctcggcgcc accatcacgg agcgcggtga gagcttctac aacccgctca ttccaaaggt gctgaatctt ctggaggagg cggaccagat cgaggtgagc gacggcgcga agctcatcat cagccgggag gagcgcaagc tggatagcct cgattcccgt gacatgacga agctagcgag tcagcacctc gcgtttctct cacgcgatgg ttcgtcgtac cacccgaatc tgattgccgc catgaagaag gttggcatct tgaccggggc agagggtgaa gagtcggtgg tgctgtcgaa gaaggaggtg aagctgtgga agaagtttga tatccgcatg gacctggaca agctgatggc ccagctcgcc cccctatata aaaagcagct cgaccccctc ttcctggagg cgttcaaggc tgcggggatt gtcaagggcg actgcatcct tgtcccccgc ttctccttcc ctctgatggt tgtgaagagc gatggtggct atacctacga cacgaccgat gtcacagcca cgtaccaccg cttcgtcgtc gagaagatgg accgtgttat ctactgcacc gatgtgggtc agtacgagca ctttcgcatg tgcctgcaga cggcgaagga catgggctgg atggaacacg ctacgtggga ccacgccggc tttggtctcg tgacaggggc agatggcaag aagatcaaga cgcgctcggg tgagacagca aagctgaagg accttatgga cgaggctgtg gagcgctccc tcgcgatcct gaaggagcgc gaggcgggcg agcgcagcca aggccactcg gaagaagaga tgcagacgct atccaacatt atcggtattg gcgccatcaa gtactttgat ctcaagcaga cacgcaccag tgactacgcc tttagctacg acaagatgct ggacatgtct ggcaacacag ccgtgttcct tctttaccag tacgctcgca tctgctctat caagcgcaag gctggtgtgg cggaggagga gctgctcaag atcaccgaca tctctctcga gaccccgcag gagaagaacc tcgcgctctg cgcgctgcgc ttccagacgg tgattctcaa ggccgtcgag gacctcttcc cgcaccatct cgcagacttt gcgtacgagc tcatgacaaa cttctccacc ttcttccaac actgccgcgt cgtggacgac ccgctgcaga acagccgcct ctgcctcatc gagctcaccc gcatcacact caaaaagacg ctggagctgc tggccatcga gacagccgag cgcctttag
Details for LebrA.00164.a.B1.GE42517
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 79kDa
OBSERVED MW: 80kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMR TAAATVNVEL ALKEIVQATI TKAFPLVPAQ EPLITLGKVS EYQCNNAMGL VKVLSQQPQS IKMSPQMVGE ELKKNLVEND IIDEFEPTTK GFINISIRKE WVAQMVNEVL NQGIRPPVVK KQRVLVDFSS PNIAKEMHVG HLRSTIIGES ISRLLEFCLH DVSRINHIGD WGTAFGMLIL YIKRKFPDYT TNPPAISDLT AFYRAAKKCF DEDQQFKEQA RLEVVKLQAL EEDSIQAWKY ICDVSREEFN GVYARLGATI TERGESFYNP LIPKVLNLLE EADQIEVSDG AKLIISREER KLDSLDSRDM TKLASQHLAF LSRDGSSYHP NLIAAMKKVG ILTGAEGEES VVLSKKEVKL WKKFDIRMDL DKLMAQLAPL YKKQLDPLFL EAFKAAGIVK GDCILVPRFS FPLMVVKSDG GYTYDTTDVT ATYHRFVVEK MDRVIYCTDV GQYEHFRMCL QTAKDMGWME HATWDHAGFG LVTGADGKKI KTRSGETAKL KDLMDEAVER SLAILKEREA GERSQGHSEE EMQTLSNIIG IGAIKYFDLK QTRTSDYAFS YDKMLDMSGN TAVFLLYQYA RICSIKRKAG VAEEELLKIT DISLETPQEK NLALCALRFQ TVILKAVEDL FPHHLADFAY ELMTNFSTFF QHCRVVDDPL QNSRLCLIEL TRITLKKTLE LLAIETAERL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgca ccgctgccgc taccgtgaat gtggagctgg cgctgaagga gattgtccag gcgaccatta ccaaggcctt cccgctcgtc ccggcccagg agcctctcat cacccttggt aaggttagcg agtatcagtg caacaatgcc atgggactgg tgaaggtgct ctctcagcag cctcagtcaa taaaaatgag cccgcagatg gtcggcgagg agctcaagaa gaacttggtg gagaacgaca taatcgacga gttcgaaccc acgaccaagg gcttcatcaa tatcagcatc cgcaaggaat gggtggctca gatggtgaac gaggtgctga accagggcat tcgtccacct gtcgtgaaga agcagcgcgt gctcgtcgat ttttcgtcgc cgaacatcgc caaggagatg cacgtcggac atctccgctc tactatcatc ggtgagtcga tcagccgtct cctggagttc tgtctgcacg acgtttcacg cataaaccac atcggtgact ggggcacggc gttcggcatg ctcattctgt acatcaagcg caagttcccc gactacacca cgaacccgcc ggccatctcc gacctcaccg ccttctaccg cgcggcgaag aagtgcttcg acgaggacca gcagttcaag gagcaggccc gcctcgaggt ggtgaagctg caggcgctgg aggaggactc tatccaagcg tggaagtaca tctgcgacgt gtcgcgcgag gagttcaacg gggtctacgc ccgcctcggc gccaccatca cggagcgcgg tgagagcttc tacaacccgc tcattccaaa ggtgctgaat cttctggagg aggcggacca gatcgaggtg agcgacggcg cgaagctcat catcagccgg gaggagcgca agctggatag cctcgattcc cgtgacatga cgaagctagc gagtcagcac ctcgcgtttc tctcacgcga tggttcgtcg taccacccga atctgattgc cgccatgaag aaggttggca tcttgaccgg ggcagagggt gaagagtcgg tggtgctgtc gaagaaggag gtgaagctgt ggaagaagtt tgatatccgc atggacctgg acaagctgat ggcccagctc gcccccctat ataaaaagca gctcgacccc ctcttcctgg aggcgttcaa ggctgcgggg attgtcaagg gcgactgcat ccttgtcccc cgcttctcct tccctctgat ggttgtgaag agcgatggtg gctataccta cgacacgacc gatgtcacag ccacgtacca ccgcttcgtc gtcgagaaga tggaccgtgt tatctactgc accgatgtgg gtcagtacga gcactttcgc atgtgcctgc agacggcgaa ggacatgggc tggatggaac acgctacgtg ggaccacgcc ggctttggtc tcgtgacagg ggcagatggc aagaagatca agacgcgctc gggtgagaca gcaaagctga aggaccttat ggacgaggct gtggagcgct ccctcgcgat cctgaaggag cgcgaggcgg gcgagcgcag ccaaggccac tcggaagaag agatgcagac gctatccaac attatcggta ttggcgccat caagtacttt gatctcaagc agacacgcac cagtgactac gcctttagct acgacaagat gctggacatg tctggcaaca cagccgtgtt ccttctttac cagtacgctc gcatctgctc tatcaagcgc aaggctggtg tggcggagga ggagctgctc aagatcaccg acatctctct cgagaccccg caggagaaga acctcgcgct ctgcgcgctg cgcttccaga cggtgattct caaggccgtc gaggacctct tcccgcacca tctcgcagac tttgcgtacg agctcatgac aaacttctcc accttcttcc aacactgccg cgtcgtggac gacccgctgc agaacagccg cctctgcctc atcgagctca cccgcatcac actcaaaaag acgctggagc tgctggccat cgagacagcc gagcgccttt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for LebrA.00164.a.B1.PW38604
PURIFICATION DATe: 2/18/2019
CONCENTRATION: 16.41mg/ml
OBSERVED MW: 75kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 2
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMR TAAATVNVEL ALKEIVQATI TKAFPLVPAQ EPLITLGKVS EYQCNNAMGL VKVLSQQPQS IKMSPQMVGE ELKKNLVEND IIDEFEPTTK GFINISIRKE WVAQMVNEVL NQGIRPPVVK KQRVLVDFSS PNIAKEMHVG HLRSTIIGES ISRLLEFCLH DVSRINHIGD WGTAFGMLIL YIKRKFPDYT TNPPAISDLT AFYRAAKKCF DEDQQFKEQA RLEVVKLQAL EEDSIQAWKY ICDVSREEFN GVYARLGATI TERGESFYNP LIPKVLNLLE EADQIEVSDG AKLIISREER KLDSLDSRDM TKLASQHLAF LSRDGSSYHP NLIAAMKKVG ILTGAEGEES VVLSKKEVKL WKKFDIRMDL DKLMAQLAPL YKKQLDPLFL EAFKAAGIVK GDCILVPRFS FPLMVVKSDG GYTYDTTDVT ATYHRFVVEK MDRVIYCTDV GQYEHFRMCL QTAKDMGWME HATWDHAGFG LVTGADGKKI KTRSGETAKL KDLMDEAVER SLAILKEREA GERSQGHSEE EMQTLSNIIG IGAIKYFDLK QTRTSDYAFS YDKMLDMSGN TAVFLLYQYA RICSIKRKAG VAEEELLKIT DISLETPQEK NLALCALRFQ TVILKAVEDL FPHHLADFAY ELMTNFSTFF QHCRVVDDPL QNSRLCLIEL TRITLKKTLE LLAIETAERL
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGCA CCGCTGCCGC TACCGTGAAT GTGGAGCTGG CGCTGAAGGA GATTGTCCAG GCGACCATTA CCAAGGCCTT CCCGCTCGTC CCGGCCCAGG AGCCTCTCAT CACCCTTGGT AAGGTTAGCG AGTATCAGTG CAACAATGCC ATGGGACTGG TGAAGGTGCT CTCTCAGCAG CCTCAGTCAA TAAAAATGAG CCCGCAGATG GTCGGCGAGG AGCTCAAGAA GAACTTGGTG GAGAACGACA TAATCGACGA GTTCGAACCC ACGACCAAGG GCTTCATCAA TATCAGCATC CGCAAGGAAT GGGTGGCTCA GATGGTGAAC GAGGTGCTGA ACCAGGGCAT TCGTCCACCT GTCGTGAAGA AGCAGCGCGT GCTCGTCGAT TTTTCGTCGC CGAACATCGC CAAGGAGATG CACGTCGGAC ATCTCCGCTC TACTATCATC GGTGAGTCGA TCAGCCGTCT CCTGGAGTTC TGTCTGCACG ACGTTTCACG CATAAACCAC ATCGGTGACT GGGGCACGGC GTTCGGCATG CTCATTCTGT ACATCAAGCG CAAGTTCCCC GACTACACCA CGAACCCGCC GGCCATCTCC GACCTCACCG CCTTCTACCG CGCGGCGAAG AAGTGCTTCG ACGAGGACCA GCAGTTCAAG GAGCAGGCCC GCCTCGAGGT GGTGAAGCTG CAGGCGCTGG AGGAGGACTC TATCCAAGCG TGGAAGTACA TCTGCGACGT GTCGCGCGAG GAGTTCAACG GGGTCTACGC CCGCCTCGGC GCCACCATCA CGGAGCGCGG TGAGAGCTTC TACAACCCGC TCATTCCAAA GGTGCTGAAT CTTCTGGAGG AGGCGGACCA GATCGAGGTG AGCGACGGCG CGAAGCTCAT CATCAGCCGG GAGGAGCGCA AGCTGGATAG CCTCGATTCC CGTGACATGA CGAAGCTAGC GAGTCAGCAC CTCGCGTTTC TCTCACGCGA TGGTTCGTCG TACCACCCGA ATCTGATTGC CGCCATGAAG AAGGTTGGCA TCTTGACCGG GGCAGAGGGT GAAGAGTCGG TGGTGCTGTC GAAGAAGGAG GTGAAGCTGT GGAAGAAGTT TGATATCCGC ATGGACCTGG ACAAGCTGAT GGCCCAGCTC GCCCCCCTAT ATAAAAAGCA GCTCGACCCC CTCTTCCTGG AGGCGTTCAA GGCTGCGGGG ATTGTCAAGG GCGACTGCAT CCTTGTCCCC CGCTTCTCCT TCCCTCTGAT GGTTGTGAAG AGCGATGGTG GCTATACCTA CGACACGACC GATGTCACAG CCACGTACCA CCGCTTCGTC GTCGAGAAGA TGGACCGTGT TATCTACTGC ACCGATGTGG GTCAGTACGA GCACTTTCGC ATGTGCCTGC AGACGGCGAA GGACATGGGC TGGATGGAAC ACGCTACGTG GGACCACGCC GGCTTTGGTC TCGTGACAGG GGCAGATGGC AAGAAGATCA AGACGCGCTC GGGTGAGACA GCAAAGCTGA AGGACCTTAT GGACGAGGCT GTGGAGCGCT CCCTCGCGAT CCTGAAGGAG CGCGAGGCGG GCGAGCGCAG CCAAGGCCAC TCGGAAGAAG AGATGCAGAC GCTATCCAAC ATTATCGGTA TTGGCGCCAT CAAGTACTTT GATCTCAAGC AGACACGCAC CAGTGACTAC GCCTTTAGCT ACGACAAGAT GCTGGACATG TCTGGCAACA CAGCCGTGTT CCTTCTTTAC CAGTACGCTC GCATCTGCTC TATCAAGCGC AAGGCTGGTG TGGCGGAGGA GGAGCTGCTC AAGATCACCG ACATCTCTCT CGAGACCCCG CAGGAGAAGA ACCTCGCGCT CTGCGCGCTG CGCTTCCAGA CGGTGATTCT CAAGGCCGTC GAGGACCTCT TCCCGCACCA TCTCGCAGAC TTTGCGTACG AGCTCATGAC AAACTTCTCC ACCTTCTTCC AACACTGCCG CGTCGTGGAC GACCCGCTGC AGAACAGCCG CCTCTGCCTC ATCGAGCTCA CCCGCATCAC ACTCAAAAAG ACGCTGGAGC TGCTGGCCAT CGAGACAGCC GAGCGCCTTT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT