LebrA.00145.a

aspartyl-tRNA synthetase, putative

CENTER ID: LebrA.00145.a
ORGANISM: Leishmania braziliensis MHOM/BR/75/M2904
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
LebrA.00145.a.B2.GE42516 soluble domain 27 644
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
EuPathDB: TriTrypDB:LbrM.21.1130
UniProt: A4HBV0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLRVSLSSCV LWRLHGAVGT ASAAVAASVV VTSSSVMSDE WASPPSSLVS ARRRCSASTC SVHGTSSSTL PHCPASSPSS TPSTASMETA ILTSGATSGV DPGSACLTAH GVSVASLRSA FSGSCGESGR IREGDTVTVR ARLERTRSRG KRLAFLHLRQ PPLESVQAVC EGKALTKQAR NITPESIVDV TGVLCRAATS VQSTTCQGWE LQVTALQVVS QATTPLPFPY HDTNTKLDTR LNHRVMDLRT EQMAATSRLV SALGQSFRNE LLALDFIEVH TPKLLGAASE GGSNVFQVDY FDRKAYLAQS PQLHKQMMLM GDAMRVFEVG PVFRAEKSLT HRHLTEFIGL DGEMVIKDSH TEVLDVLEPV MCAVLAQLAQ KHSNLIDAVW KQQQQQQQGL ELENSDGGPQ PDPATEPPRC DGRGNAQRDV CCEVALERIE FLGITTDASP TAQTPFKISA QGDPYHARVG GDGSGCRVLR MSFANAAQLL VSCGESEGVV DGALPADDFT LPQERRLGQL IKERYGVDLY VVDGFPSSAR PFYTMPVDPS TPDGPTRSYD MYLRGEEICS GAQRVHEIEL LEQRLLAKRV DRVGLKDYVD SFRYGAWPHG GFGLGLERIA LLFLGLNDIR QVSLFPRDPK RISP
NT Sequence
atgctgcgag tttcactgtc gtcgtgcgtt ttatggcgtc tgcacggtgc cgtagggacc gcttcggctg ctgtggcagc ctctgtcgtc gttacgtcct cttcagtgat gtccgacgaa tgggcgtctc ccccttcctc actggtctcc gcgcggcgcc gctgtagtgc gagcacctgc agcgtccacg gtacttccag cagcacattg cctcattgcc ccgcctcgtc tccatcctct actccatcta ctgcctcgat ggagacggcc atattgacca gtggtgctac ttcgggagtc gaccctggca gcgcgtgtct gacggcacac ggtgtctccg ttgcttctct acgttctgcc tttagcggca gctgcggtga gagcggccgc attcgcgagg gcgacacggt caccgtgcgg gctcgcttag agcggacgcg cagccgcggc aagcgccttg cctttctcca cctccgtcag cctccccttg agtccgtgca ggctgtgtgc gaaggtaagg cgctgacgaa gcaggcgagg aacattacgc cagagtcaat tgtcgatgtc acaggggtct tgtgccgcgc agccacctct gtgcagtcga ccacgtgcca ggggtgggag ctgcaggtga cggcgctgca ggtcgtcagt caagcgacca ccccactgcc atttccatac cacgacacga acacaaagct ggacacacgc ctcaatcacc gcgtcatgga cctccgcaca gagcaaatgg ccgcgacgtc acggctcgtg tcggcgctcg ggcagagctt ccgcaacgag cttctcgcac tcgacttcat cgaggtgcac acaccgaagc tccttggggc cgcgtcggag ggaggcagta acgtgtttca ggtggactac tttgatcgca aggcgtacct ggctcaatca ccgcagctgc acaagcagat gatgctgatg ggcgacgcga tgcgcgtgtt tgaggtcggg ccggtgtttc gcgctgagaa gagtctgaca caccggcact tgacagagtt cattggtctc gatggtgaga tggtgatcaa agatagtcac acggaggtgc tagacgtcct ggagcccgtc atgtgtgccg ttctggcgca gctcgcacag aagcacagca accttatcga tgctgtctgg aagcagcagc agcagcagca gcaggggtta gagctggaaa acagcgacgg tggtccacag cctgaccctg ctacggagcc gcctcgttgt gacggccgcg gtaacgcgca gcgtgatgtg tgctgcgagg tagcgctgga gcggatcgag tttttaggaa tcactacgga tgcctctcca acggcgcaga caccattcaa aatctccgcg caaggcgatc catatcacgc tcgcgtgggc ggtgacggta gcgggtgccg tgtgcttcgc atgagctttg caaatgcggc acagctgctg gtgagctgcg gtgagtcaga gggcgtagtt gacggcgcac tgcccgctga tgactttacg ctgccgcagg agcgccgcct tggccaactc ataaaagagc gctacggggt tgacctctac gtggtcgatg ggtttccctc ctccgccaga cccttttaca cgatgccggt tgacccatca acgccggatg gcccgacgcg gagctacgat atgtacctac gtggggaaga gatctgcagt ggtgcacagc gtgtccatga gatagagctg ctggaacaac gactcttggc gaagcgcgtg gacagggtgg gtctgaaaga ctacgttgac tccttccgct acggcgcgtg gccacacggc ggcttcggtc tggggctcga gcgcattgct ctcttgtttc ttggcctcaa cgacatccgg caggtgtcac tgtttccacg cgacccgaag cggattagtc catga
Details for LebrA.00145.a.B2.GE42516
HARVESTED ON: 7/3/2018
SEQUENCED ON: 7/10/2018
EXPECTED MW: 68kDa
OBSERVED MW: 68kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 75
PERCENT COVERAGE: 53
Validated AA Sequence
MAHHHHHHMA SVVVTSSSVM SEEWASPPSS LVSARRRCSA STCSVHGASS STSPHCPASS PSSTPSTASM ETTILTSGAT SGVDPGSECL TAHGVSIASL RSAFSGSCGE SGRIREGDTV TVRARLERTR SRGKRLAFLH LRQPPLESVQ AVCEGKALTK QARNITPESI VDVTGVLCRA ATSVQSTTCQ GWELQVSALQ IVSQATTPLP FPYHDTNTKL DTRLNHRVMD LRTEXXAATS RLVXALGQSF RNELLALXFI EVXXXXXXGX XXXGGXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXTXXXXS SLXXMXRW
Validated NT Sequence
ccatggctca ccaccaccac caccatatgg cctctgtcgt cgttacgtct tcttcagtga tgtccgagga atgggcgtct cccccttcct cactggtctc cgcgcggcgc cgctgtagtg cgagcacctg cagcgtccac ggtgcttcca gcagcacatc gcctcattgc cccgcctcgt ctccatcctc tactccatct actgcctcga tggagacgac catattgacc agtggtgcta cttcgggagt cgaccctggc agcgagtgtc tgacggcaca cggtgtctcc attgcttctc tacgttctgc ctttagcggc agctgcggtg agagcggccg cattcgcgag ggcgacacag tcaccgtgcg ggctcgctta gagcggacgc gcagccgcgg caagcgcctt gcctttctcc acctccgtca gcctcccctt gagtccgtgc aggctgtgtg cgaaggtaag gcgctgacga agcaggcgag gaacattacg ccagagtcaa ttgtcgatgt cacaggggtc ttgtgccgcg cagccacctc tgtgcagtcg accacgtgcc aggggtggga gctgcaggtg tcggcgctgc agatcgtcag tcaagcgacc accccactgc catttccata ccacgacacg aacacaaagc tggacacacg cctcaatcac cgcgtcatgg acctccgcac agagcnnntn gccgcgacgt cacggctcgt gtnggcgctc gggcagagct tccgcaacga gcttctcgca ctnnnnttca tcgaggtgnn nnnnncgaan nnnnttgggn nnnnnnnnna nggaggcann nnnnnnttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnann nnnnnnnnnn annnnnnnnn nnnnngnnnn nnatnnnnnn nnnnnnnnnn nnntnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncacnnnn nnnnnnnnga gttcattggn nncgatggng agatggtgat caaagnnngt cacacggagg tgctagacgt cctggagccc gtcatgtgtg ccgttctgnc gcagcttgca cagaagcaca gcaaccttat cgatgctgtc tggaagcagc agcagcagca gcaggggtca gagctggaaa acagcgacgg tgatccacag cctgaccctg ctacggagcc gcctcgttgt gacggccgcg gtaacgcgca gcgtgatgtg tgctgcgagg tagcgctgga gcggatcgag tttctgggaa tcactacgga tgcctctcca acggcgcaga caccattcaa aatctccgcg caaggtgatc catatcacgc tcgcgtgggc ggtgacggta gcgggtgccg tgtgcttcgc atgagctttg caaatgcggc acagctgctg gtgagctgcg gtgagtcaga gggcgtagtt gactgcgcac cgcccgctga tgactttacg ctgccgcagg agcgccgcct tggccaactc ataaaagagc gttacggggt tgacctctac gtggtcgatg ggtttccctc cgccgccaga cccttttaca cgatgccggt tgacccatca acgccggatg gcccgacgcg gagctacgat atgtacctac gtggggaaga gatctgcagt ggcgcacagc gtgtccatga gatagagctg ctggaacaac gactcttggc gaagaaggtg gacagggtgg gtctgaaaga ctacgttgac tccttccgct acggcgcgtg gccacacggc ggcttcggtc tggggctcga gcgcattgct ctcttgtttc ttggtctcaa cgacatccgg caggtgtcac tgtttccac
Expected Protein Sequence
MAHHHHHHAS VVVTSSSVMS DEWASPPSSL VSARRRCSAS TCSVHGTSSS TLPHCPASSP SSTPSTASME TAILTSGATS GVDPGSACLT AHGVSVASLR SAFSGSCGES GRIREGDTVT VRARLERTRS RGKRLAFLHL RQPPLESVQA VCEGKALTKQ ARNITPESIV DVTGVLCRAA TSVQSTTCQG WELQVTALQV VSQATTPLPF PYHDTNTKLD TRLNHRVMDL RTEQMAATSR LVSALGQSFR NELLALDFIE VHTPKLLGAA SEGGSNVFQV DYFDRKAYLA QSPQLHKQMM LMGDAMRVFE VGPVFRAEKS LTHRHLTEFI GLDGEMVIKD SHTEVLDVLE PVMCAVLAQL AQKHSNLIDA VWKQQQQQQQ GLELENSDGG PQPDPATEPP RCDGRGNAQR DVCCEVALER IEFLGITTDA SPTAQTPFKI SAQGDPYHAR VGGDGSGCRV LRMSFANAAQ LLVSCGESEG VVDGALPADD FTLPQERRLG QLIKERYGVD LYVVDGFPSS ARPFYTMPVD PSTPDGPTRS YDMYLRGEEI CSGAQRVHEI ELLEQRLLAK RVDRVGLKDY VDSFRYGAWP HGGFGLGLER IALLFLGLND IRQVSLFPRD PKRISP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcctctg tcgtcgttac gtcctcttca gtgatgtccg acgaatgggc gtctccccct tcctcactgg tctccgcgcg gcgccgctgt agtgcgagca cctgcagcgt ccacggtact tccagcagca cattgcctca ttgccccgcc tcgtctccat cctctactcc atctactgcc tcgatggaga cggccatatt gaccagtggt gctacttcgg gagtcgaccc tggcagcgcg tgtctgacgg cacacggtgt ctccgttgct tctctacgtt ctgcctttag cggcagctgc ggtgagagcg gccgcattcg cgagggcgac acggtcaccg tgcgggctcg cttagagcgg acgcgcagcc gcggcaagcg ccttgccttt ctccacctcc gtcagcctcc ccttgagtcc gtgcaggctg tgtgcgaagg taaggcgctg acgaagcagg cgaggaacat tacgccagag tcaattgtcg atgtcacagg ggtcttgtgc cgcgcagcca cctctgtgca gtcgaccacg tgccaggggt gggagctgca ggtgacggcg ctgcaggtcg tcagtcaagc gaccacccca ctgccatttc cataccacga cacgaacaca aagctggaca cacgcctcaa tcaccgcgtc atggacctcc gcacagagca aatggccgcg acgtcacggc tcgtgtcggc gctcgggcag agcttccgca acgagcttct cgcactcgac ttcatcgagg tgcacacacc gaagctcctt ggggccgcgt cggagggagg cagtaacgtg tttcaggtgg actactttga tcgcaaggcg tacctggctc aatcaccgca gctgcacaag cagatgatgc tgatgggcga cgcgatgcgc gtgtttgagg tcgggccggt gtttcgcgct gagaagagtc tgacacaccg gcacttgaca gagttcattg gtctcgatgg tgagatggtg atcaaagata gtcacacgga ggtgctagac gtcctggagc ccgtcatgtg tgccgttctg gcgcagctcg cacagaagca cagcaacctt atcgatgctg tctggaagca gcagcagcag cagcagcagg ggttagagct ggaaaacagc gacggtggtc cacagcctga ccctgctacg gagccgcctc gttgtgacgg ccgcggtaac gcgcagcgtg atgtgtgctg cgaggtagcg ctggagcgga tcgagttttt aggaatcact acggatgcct ctccaacggc gcagacacca ttcaaaatct ccgcgcaagg cgatccatat cacgctcgcg tgggcggtga cggtagcggg tgccgtgtgc ttcgcatgag ctttgcaaat gcggcacagc tgctggtgag ctgcggtgag tcagagggcg tagttgacgg cgcactgccc gctgatgact ttacgctgcc gcaggagcgc cgccttggcc aactcataaa agagcgctac ggggttgacc tctacgtggt cgatgggttt ccctcctccg ccagaccctt ttacacgatg ccggttgacc catcaacgcc ggatggcccg acgcggagct acgatatgta cctacgtggg gaagagatct gcagtggtgc acagcgtgtc catgagatag agctgctgga acaacgactc ttggcgaagc gcgtggacag ggtgggtctg aaagactacg ttgactcctt ccgctacggc gcgtggccac acggcggctt cggtctgggg ctcgagcgca ttgctctctt gtttcttggc ctcaacgaca tccggcaggt gtcactgttt ccacgcgacc cgaagcggat tagtccatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at