SSGCID: Target Page

KlpnC.20506.a

Transcription antitermination protein RfaH

CENTER ID:	KlpnC.20506.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20506.a.B1.GE44465	Full length(KlpnC.20506.a)		1	162

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1125630.4.peg.178
UniProt:	A0A0H3GPY2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQAWYLLYCK RGQLQRAQEH LERQSVNCLM PTIALEKIIR GKRTMVSEPL FPNYLFIEFD PEVIHTTTIS ATRGVNNFVR FGALPAIVPS AVIHQLSIYK PEGITDPETP HEGDSVLITD GAFEGLEAIF TEPDGEARSM LLLNLLNKQV LQSVKNTDFQ KI

NT Sequence

ATGCAAGCCT GGTACTTACT GTATTGCAAA CGTGGGCAGC TACAACGAGC CCAGGAACAT CTGGAACGTC AGTCAGTTAA CTGCCTGATG CCGACGATTG CGCTGGAAAA AATCATTCGC GGTAAACGCA CCATGGTCAG CGAACCGTTG TTCCCGAACT ACCTGTTTAT TGAGTTCGAT CCAGAGGTGA TTCATACCAC CACCATCAGC GCCACTCGCG GGGTTAACAA CTTCGTGCGC TTTGGCGCCC TACCCGCTAT CGTCCCCTCG GCGGTCATCC ATCAGCTTTC TATCTACAAA CCGGAAGGGA TTACCGACCC GGAAACGCCG CATGAGGGCG ATAGCGTGCT AATCACCGAC GGCGCGTTTG AAGGCCTGGA GGCGATCTTC ACCGAGCCCG ACGGCGAAGC GCGCTCTATG TTGCTGTTAA ACCTGCTGAA TAAGCAAGTC CTGCAGAGCG TTAAAAATAC CGACTTCCAG AAAATC

Details for KlpnC.20506.a.B1.GE44465

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	19kDa
OBSERVED MW:	19kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMQ AWYLLYCKRG QLQRAQEHLE RQSVNCLMPT IALEKIIRGK RTMVSEPLFP NYLFIEFDPE VIHTTTISAT RGVNNFVRFG ALPAIVPSAV IHQLSIYKPE GITDPETPHE GDSVLITDGA FEGLEAIFTE PDGEARSMLL LNLLNKQVLQ SVKNTDFQKI

Validated NT Sequence

atcttactca cttagatttt ctggaagtcg gtatttttaa cgctctgcag gacttgctta ttcagcaggt ttaacagcaa catagagcgc gcttcgccgt cgggctcggt gaagatcgcc tccaggcctt caaacgcgcc gtcggtgatt agcacgctat cgccctcatg cggcgtttcc gggtcggtaa tcccttccgg tttgtagata gaaagctgat ggatgaccgc cgaggggacg atagcgggta gggcgccaaa gcgcacgaag ttgttaaccc cgcgagtggc gctgatggtg gtggtatgaa tcacctctgg atcgaactca ataaacaggt agttcgggaa caacggttcg ctgaccatgg tgcgtttacc gcgaatgatt ttttccagcg caatcgtcgg catcaggcag ttaactgact gacgttccag atgttcctgg gctcgttgta gctgcccacg tttgcaatac agtaagtacc aggcttgcat atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHMQ AWYLLYCKRG QLQRAQEHLE RQSVNCLMPT IALEKIIRGK RTMVSEPLFP NYLFIEFDPE VIHTTTISAT RGVNNFVRFG ALPAIVPSAV IHQLSIYKPE GITDPETPHE GDSVLITDGA FEGLEAIFTE PDGEARSMLL LNLLNKQVLQ SVKNTDFQKI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag cctggtactt actgtattgc aaacgtgggc agctacaacg agcccaggaa catctggaac gtcagtcagt taactgcctg atgccgacga ttgcgctgga aaaaatcatt cgcggtaaac gcaccatggt cagcgaaccg ttgttcccga actacctgtt tattgagttc gatccagagg tgattcatac caccaccatc agcgccactc gcggggttaa caacttcgtg cgctttggcg ccctacccgc tatcgtcccc tcggcggtca tccatcagct ttctatctac aaaccggaag ggattaccga cccggaaacg ccgcatgagg gcgatagcgt gctaatcacc gacggcgcgt ttgaaggcct ggaggcgatc ttcaccgagc ccgacggcga agcgcgctct atgttgctgt taaacctgct gaataagcaa gtcctgcaga gcgttaaaaa taccgacttc cagaaaatct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat