SSGCID: Target Page

KlpnC.20505.a

Penicillin-insensitive murein endopeptidase

CENTER ID:	KlpnC.20505.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20505.a.B2.GE44464	Mature protein(KlpnC.20505.a)		19	273

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.3691
UniProt:	A0A0H3GR96

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNTVIALLA LLASAGSLAA TPWQKITQPI GGSAQSIGAF SNGCIVGAEA LPLSAPGYQV MRTDQRRYFG HPDLVQFIQR LSNQVHNKGM GTVLIGDMGM PAGGRFNGGH ASHQTGLDVD IFLQLPQTRW TSSQLLKPQA LDLVASDGKH VVPSLWSPQI SQLIKLAAED SEVTRIFVNP AIKQQLCLDA GSDRQWLRKV RPWFQHRAHM HVRLRCPAGS LECEDQAPPP PGDGCGAELQ SWFEPPKPGS TPPVKKTPPL PPSCQALLDE HVL

NT Sequence

ATGAAAAACA CCGTTATCGC ACTGCTTGCG CTGTTGGCTA GCGCCGGTAG CCTGGCCGCC ACGCCATGGC AGAAAATAAC CCAACCGATC GGCGGCAGCG CCCAGTCAAT CGGCGCGTTT TCCAATGGCT GTATCGTTGG CGCCGAGGCG CTCCCCCTGA GTGCCCCCGG CTATCAGGTG ATGCGTACCG ATCAGCGTCG CTACTTCGGT CATCCGGATC TGGTTCAGTT TATTCAGCGC CTGAGTAATC AGGTGCACAA TAAAGGAATG GGAACCGTGC TGATTGGCGA CATGGGGATG CCGGCGGGCG GGCGCTTTAA CGGCGGGCAC GCCAGCCACC AGACGGGCCT CGATGTCGAT ATCTTCCTTC AGCTGCCGCA AACGCGCTGG ACATCGTCCC AGCTGTTGAA GCCGCAGGCG CTGGACCTGG TCGCCAGCGA TGGCAAGCAT GTGGTGCCGT CGCTATGGAG CCCGCAAATC AGCCAGCTTA TCAAGCTGGC GGCGGAAGAT AGCGAAGTTA CCCGCATCTT CGTCAACCCG GCGATTAAAC AGCAGCTGTG CCTGGACGCG GGCAGCGACC GCCAGTGGCT GCGTAAAGTC CGTCCCTGGT TCCAGCATCG CGCTCATATG CACGTCCGAC TGCGTTGCCC GGCGGGAAGT CTGGAGTGCG AGGATCAGGC GCCGCCGCCG CCCGGCGATG GCTGCGGAGC GGAGCTGCAG AGCTGGTTCG AACCACCTAA ACCCGGGTCT ACCCCCCCTG TGAAGAAGAC GCCGCCGCTG CCGCCTTCCT GCCAGGCGCT ACTGGATGAG CATGTGCTG

Details for KlpnC.20505.a.B2.GE44464

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHXHXHMA ATPXQKITQP IGGSAQSIGA FSNGCIVGAE ALPLSAPGYQ VMRTDQRRYF GHPDLVQFIQ RLSNQVHNKG MGTVLIGDMG MPAGGRFNGG HASHQTGLDV DIFLQLPQTR WTSSQLLKPQ ALDLVASDGK HVVPSLWSPQ ISQLIKLAAE DSEVTRIFVN PAIKQQLCLD AGSDRQWLRK VRPWFQHRAH MHVRLRCPAG SLECEDQAPP PPGDGCGAEL QSWFEPPKPG STPPVKKTPP PLPPSCQALL DEHVL

Validated NT Sequence

atcttactca cttacagcac atgctcatcc agtagcgcct ggcaggaagg cggcagcggc ggcggcgtct tcttcacagg gggggtagac ccgggtttag gtggttcgaa ccagctctgc agctccgctc cgcagccatc gccgggcggc ggcggcgcct gatcctcgca ctccagactt cccgccgggc aacgcagtcg gacatgcata tgagcgcgat gctggaacca gggacggact ttacgcagcc actggcggtc gctgcccgcg tccaggcaca gctgctgttt aatcgccggg ttgacgaaga tgcgggtaac ttcgctatct tccgccgcca gcttgataag ctggctgatt tgcgggctcc atagcgacgg caccacatgc ttgccatcgc tggcgaccag gtccagcgcc tgcggcttca acagctggga cgatgtccag cgcgtttgcg gcagctgaag gaagatatcg acatcgaggc ccgtctggtg gctggcgtgc ccgccgttaa agcgcccgcc cgccggcatc cccatgtcgc caatcagcac ggttcccatt cctttattgt gcacctgatt actcaggcgc tgaataaact gaaccagatc cggatgaccg aagtagcgac gctgatcggt acgcatcacc tgatagccgg gggcactcag ggggagcgcc tcggcgccaa cgatacagcc attggaaaac gcgccgattg actgggcgct gccgccgatc ggttgggtta ttttctgnca tggcgtggcg gccatatggn ggtggnggtg gtgagccat

Expected Protein Sequence

MAHHHHHHAA TPWQKITQPI GGSAQSIGAF SNGCIVGAEA LPLSAPGYQV MRTDQRRYFG HPDLVQFIQR LSNQVHNKGM GTVLIGDMGM PAGGRFNGGH ASHQTGLDVD IFLQLPQTRW TSSQLLKPQA LDLVASDGKH VVPSLWSPQI SQLIKLAAED SEVTRIFVNP AIKQQLCLDA GSDRQWLRKV RPWFQHRAHM HVRLRCPAGS LECEDQAPPP PGDGCGAELQ SWFEPPKPGS TPPVKKTPPL PPSCQALLDE HVL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgcca cgccatggca gaaaataacc caaccgatcg gcggcagcgc ccagtcaatc ggcgcgtttt ccaatggctg tatcgttggc gccgaggcgc tccccctgag tgcccccggc tatcaggtga tgcgtaccga tcagcgtcgc tacttcggtc atccggatct ggttcagttt attcagcgcc tgagtaatca ggtgcacaat aaaggaatgg gaaccgtgct gattggcgac atggggatgc cggcgggcgg gcgctttaac ggcgggcacg ccagccacca gacgggcctc gatgtcgata tcttccttca gctgccgcaa acgcgctgga catcgtccca gctgttgaag ccgcaggcgc tggacctggt cgccagcgat ggcaagcatg tggtgccgtc gctatggagc ccgcaaatca gccagcttat caagctggcg gcggaagata gcgaagttac ccgcatcttc gtcaacccgg cgattaaaca gcagctgtgc ctggacgcgg gcagcgaccg ccagtggctg cgtaaagtcc gtccctggtt ccagcatcgc gctcatatgc acgtccgact gcgttgcccg gcgggaagtc tggagtgcga ggatcaggcg ccgccgccgc ccggcgatgg ctgcggagcg gagctgcaga gctggttcga accacctaaa cccgggtcta ccccccctgt gaagaagacg ccgccgctgc cgccttcctg ccaggcgcta ctggatgagc atgtgctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat