KlpnC.20499.a

Dihydropteridine reductase/oxygen-insensitive NAD(P)H nitroreductase

CENTER ID:	KlpnC.20499.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	in PDB
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20499.a.B1.GE44458	Full length(KlpnC.20499.a)		1	217

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Structures

8DOR

DEPOSITED: 7/14/2022

DETERMINATION: XRay

Diffraction Image

CLONE: KlpnC.20499.a.B1.GE44458

PROTEIN: KlpnC.20499.a.B1.PW39082

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1125630.4.peg.1345
UniProt:	A0A0H3GL02

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDIVSVALKR YSTKAFDATK KLTAGEAEQL KTLLQYSPSS TNSQPWHFIV ASTDEGKARV AKAASGTYVF NERKILDASH VVVFCAKTAM DDAWLQRVVD QEEADGRFAT PDAKAANHKG RTFFADMHRK ELKDDDQWMA KQVYLNVGNF LLGVAAMGLD AVPIEGVDFA ILDEEFDLKA QGYTSLVVVR VGHHSVEDFN ATLPKSRLPQ STTITEI

NT Sequence

ATGGATATCG TATCGGTCGC TTTGAAGCGC TATTCCACCA AGGCCTTTGA CGCCACGAAA AAGCTGACTG CCGGGGAAGC GGAACAGCTG AAAACGCTGC TGCAGTACAG CCCGTCTAGC ACCAACTCCC AGCCGTGGCA CTTTATTGTC GCCAGCACCG ATGAAGGGAA AGCTCGCGTG GCGAAAGCCG CCAGCGGCAC CTACGTGTTC AACGAACGTA AAATTCTTGA CGCCTCGCAC GTGGTGGTGT TCTGCGCGAA AACCGCGATG GACGACGCCT GGCTGCAGCG CGTGGTGGAT CAGGAAGAGG CCGATGGCCG TTTCGCCACC CCGGACGCAA AAGCCGCTAA CCACAAGGGT CGCACTTTCT TTGCCGATAT GCACCGCAAA GAGCTGAAAG ATGACGATCA GTGGATGGCA AAGCAGGTGT ATCTCAACGT CGGCAATTTC CTGCTGGGCG TAGCGGCGAT GGGTCTCGAC GCCGTGCCGA TTGAAGGGGT CGATTTTGCG ATCCTCGATG AGGAATTTGA CCTGAAAGCC CAGGGCTACA CCAGCCTGGT GGTGGTCCGG GTCGGCCACC ATAGCGTAGA AGATTTCAAC GCTACCCTGC CGAAGTCGCG TCTGCCGCAG TCGACGACCA TTACCGAGAT T

Details for KlpnC.20499.a.B1.GE44458

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMD IVSVALKRYS TKAFDATKKL TAGEAEQLKT LLQYSPSSTN SQPWHFIVAS TDEGKARVAK AASGTYVFNE RKILDASHVV VFCAKTAMDD AWLQRVVDQE EADGRFATPD AKAANHKGRT FFADMHRKEL KDDDQWMAKQ VYLNVGNFLL GVAAMGLDAV PIEGVDFAIL DEEFDLKAQG YTSLVVVPVG HHSAEDFNAT LPKSRLPQST TITEI

Validated NT Sequence

atcttactca cttaaatctc ggtaatggtc gtcgactgcg gcagacgcga cttcggcagg gtagcgttga aatcttctgc gctatggtgg ccgaccggga ccaccaccag gctggtgtag ccctgggctt tcaggtcaaa ttcctcatcg aggatcgcaa aatcgacccc ttcaatcggc acggcgtcga gacccatcgc cgctacgccc agcaggaaat tgccgacgtt gagatacacc tgtttcgcca tccactgatc gtcatctttc agctctttgc ggtgcatatc ggcaaagaaa gtacgaccct tgtggttagc ggcttttgcg tccggggtgg cgaaacggcc atcggcctct tcctgatcca ccacgcgctg cagccaggcg tcgtccatcg cggttttcgc gcagaacacc accacgtgcg aggcgtcgag aattttacgt tcgttgaaca cgtaggtgcc gctggcggct ttcgccacgc gagctttccc ttcatcggtg ctggcgacaa taaagtgcca cggctgggag ttggtgctag acgggctgta ctgcagcagc gttttcagct gttccgcttc cccggcagtc agctttttcg tggcgtcaaa ggccttggtg gaatagcgct tcaaagcgac cgatacgata tccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggata tcgtatcggt cgctttgaag cgctattcca ccaaggcctt tgacgccacg aaaaagctga ctgccgggga agcggaacag ctgaaaacgc tgctgcagta cagcccgtct agcaccaact cccagccgtg gcactttatt gtcgccagca ccgatgaagg gaaagctcgc gtggcgaaag ccgccagcgg cacctacgtg ttcaacgaac gtaaaattct tgacgcctcg cacgtggtgg tgttctgcgc gaaaaccgcg atggacgacg cctggctgca gcgcgtggtg gatcaggaag aggccgatgg ccgtttcgcc accccggacg caaaagccgc taaccacaag ggtcgcactt tctttgccga tatgcaccgc aaagagctga aagatgacga tcagtggatg gcaaagcagg tgtatctcaa cgtcggcaat ttcctgctgg gcgtagcggc gatgggtctc gacgccgtgc cgattgaagg ggtcgatttt gcgatcctcg atgaggaatt tgacctgaaa gcccagggct acaccagcct ggtggtggtc cgggtcggcc accatagcgt agaagatttc aacgctaccc tgccgaagtc gcgtctgccg cagtcgacga ccattaccga gatttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat