SSGCID: Target Page

KlpnC.20498.a

Leucyl/phenylalanyl-tRNA--protein transferase

CENTER ID:	KlpnC.20498.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20498.a.B1.GE44457	Full length(KlpnC.20498.a)		1	239

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.1748
UniProt:	A0A0H3GM83

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRIEVKMRLV QLSRHSIAFP SPEGALREPN GLLALGGDLS PARLLMAYQR GIFPWFSPGD PILWWSPDPR AVLWPEQFHL SRSMKRFHQR SPYRVTLNHA FGEVIEGCAS DRDEGTWITS SIVRAYHQLH ELGHAHSIEV WQENTLVGGM YGVAQGALFC GESMFSRAEN ASKTALLVFC QDFAHSGGKL IDCQVLNNHT ASLGAVDIPR RDYLDYLSVL RGYRLPERFW VPRVLFPGG

NT Sequence

ATGCGTATTG AGGTTAAAAT GCGTCTGGTT CAGCTCTCCC GGCACTCTAT CGCCTTCCCC TCTCCGGAAG GCGCCTTGCG AGAACCTAAT GGCCTGCTGG CGCTCGGCGG CGATCTGAGC CCCGCGCGGC TGCTGATGGC CTATCAGCGT GGTATTTTTC CCTGGTTTTC CCCTGGCGAC CCGATCCTCT GGTGGTCGCC CGACCCCCGG GCGGTACTTT GGCCAGAGCA ATTCCATCTC AGCCGCAGCA TGAAGCGCTT TCATCAGCGT TCGCCCTACC GGGTCACGCT CAACCACGCC TTTGGCGAAG TGATTGAGGG CTGCGCCAGC GATCGTGACG AAGGCACCTG GATAACCAGC AGTATCGTTC GCGCCTATCA CCAGCTGCAT GAGCTGGGTC ATGCCCACTC CATTGAAGTC TGGCAGGAGA ATACCCTGGT GGGAGGAATG TACGGCGTAG CGCAGGGAGC GCTGTTTTGC GGCGAATCGA TGTTTAGCCG GGCGGAAAAT GCCTCCAAAA CCGCGCTGCT GGTATTTTGC CAGGACTTTG CGCACAGCGG CGGTAAATTA ATTGACTGTC AGGTACTGAA CAACCATACC GCGTCATTAG GCGCAGTGGA TATTCCCCGC CGCGATTATC TGGACTATCT GTCGGTATTA CGCGGCTACC GACTGCCCGA GCGATTTTGG GTGCCGCGGG TGCTATTTCC TGGCGGA

Details for KlpnC.20498.a.B1.GE44457

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMR IEVKMRLVQL SRHSIAFPSP EGALREPNGL LALGGDLSPA RLLMAYQRGI FPWFSPGDPI LWWSPDPRAV LWPEQFHLSR SMKRFHQRSP YRVTLNHAFG EVIEGCASDR DEGTWITSSI VRAYHQLHEL GHAHSIEVWQ ENTLVGGMYG VAQGALFCGE SMFSRAENAS KTALLVFCQD FAHSGGKLID CQVLNNHTAS LGAVDIPRRD YLDYLSVLRG YRLPERFWVP RVLFPGG

Validated NT Sequence

atcttactca cttatccgcc aggaaatagc acccgcggca cccaaaatcg ctcgggcagt cggtagccgc gtaataccga cagatagtcc agataatcgc ggcggggaat atccactgcg cctaatgacg cggtatggtt gttcagtacc tggcagtcaa ttaatttacc gccgctgtgc gcaaagtcct ggcaaaatac cagcagcgcg gttttggagg cattttctgc ccggctaaac atcgattcgc cgcaaaacag cgctccctgc gctacgccgt acattcctcc caccagggta ttctcctgcc agacttcaat ggagtgggca tgacccagct catgcagctg gtgataggcg cgaacgatac tgctggttat ccaggtgcct tcgtcacgat cgctggcgca gccctcaatc acttcgccaa aggcgtggtt gagcgtgacc cggtagggcg aacgctgatg aaagcgcttc atgctgcggc tgagatggaa ttgctctggc caaagtaccg cccgggggtc gggcgaccac cagaggatcg ggtcgccagg ggaaaaccag ggaaaaatac cacgctgata ggccatcagc agccgcgcgg ggctcagatc gccgccgagc gccagcaggc cattaggttc tcgcaaggcg ccttccggag aggggaaggc aatagagtgc cgggagagct gaaccagacg cattttaacc tcaatacgca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgta ttgaggttaa aatgcgtctg gttcagctct cccggcactc tatcgccttc ccctctccgg aaggcgcctt gcgagaacct aatggcctgc tggcgctcgg cggcgatctg agccccgcgc ggctgctgat ggcctatcag cgtggtattt ttccctggtt ttcccctggc gacccgatcc tctggtggtc gcccgacccc cgggcggtac tttggccaga gcaattccat ctcagccgca gcatgaagcg ctttcatcag cgttcgccct accgggtcac gctcaaccac gcctttggcg aagtgattga gggctgcgcc agcgatcgtg acgaaggcac ctggataacc agcagtatcg ttcgcgccta tcaccagctg catgagctgg gtcatgccca ctccattgaa gtctggcagg agaataccct ggtgggagga atgtacggcg tagcgcaggg agcgctgttt tgcggcgaat cgatgtttag ccgggcggaa aatgcctcca aaaccgcgct gctggtattt tgccaggact ttgcgcacag cggcggtaaa ttaattgact gtcaggtact gaacaaccat accgcgtcat taggcgcagt ggatattccc cgccgcgatt atctggacta tctgtcggta ttacgcggct accgactgcc cgagcgattt tgggtgccgc gggtgctatt tcctggcgga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat