KlpnC.20495.a

DNA-specific endonuclease I

CENTER ID: KlpnC.20495.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20495.a.B2.GE44454 Mature protein(KlpnC.20495.a) 22 235
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4345
UniProt: A0A0H3GT48

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRMHVLAVA VLSAAVSGPL AAAGINSFSQ AKAAGVKVNA DVPGDFYCGC KIDWQGKKGV IDLESCGYKV RKNENRASRV EWEHVVPAWQ FGHQRQCWQE GGRKNCAKDP EYRKMESDMH NLQPAVGEVN GDRGNFMYSQ WNGGEGQYGQ CTMKVDFKDK IAEPPARARG AIARTYFYMR DRYQLNLSRQ QTQLFTAWNK QYPVTAWECE RDERIAKVQG NHNPYVQQAC QAQKS
NT Sequence
ATGTCCCGTA TGCATGTTTT AGCCGTAGCG GTATTGAGCG CGGCAGTCAG TGGTCCGCTT GCGGCCGCTG GTATTAATAG TTTTTCCCAG GCCAAAGCCG CTGGCGTCAA AGTGAATGCC GACGTTCCGG GCGATTTTTA TTGCGGCTGT AAAATCGACT GGCAGGGGAA AAAAGGCGTT ATCGATTTGG AATCCTGCGG CTATAAAGTG CGTAAAAATG AGAACCGCGC CAGCCGGGTT GAATGGGAGC ACGTAGTGCC GGCATGGCAG TTCGGCCATC AGCGTCAGTG CTGGCAGGAG GGTGGACGTA AAAACTGCGC CAAAGATCCG GAATACCGCA AGATGGAAAG CGATATGCAC AACCTGCAGC CGGCGGTGGG AGAAGTGAAT GGCGATCGCG GCAACTTTAT GTACAGCCAG TGGAACGGCG GTGAAGGCCA GTACGGCCAG TGCACAATGA AGGTCGATTT TAAAGATAAA ATCGCCGAGC CCCCTGCCCG CGCTCGCGGC GCCATCGCCC GCACCTACTT CTATATGCGC GACCGCTATC AGCTGAATCT TTCCCGCCAG CAAACTCAGC TGTTTACCGC CTGGAATAAA CAGTACCCGG TCACCGCCTG GGAATGTGAA CGTGATGAGC GCATCGCGAA AGTGCAGGGC AACCATAATC CGTATGTCCA GCAGGCTTGC CAGGCGCAAA AGAGC
Details for KlpnC.20495.a.B2.GE44454
HARVESTED ON: data unavailable
SEQUENCED ON: 7/22/2021
EXPECTED MW: 26kDa
OBSERVED MW: 15kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA AGINSFSQAK AAGVKVNADV PGDFYCGCKI DWQGKKGVID LESCGYKVRK NENRASRVEW EHVVPAWQFG HQRQCWQEGG RKNCAKDPEY RKMESDMHNL QPAVGEVNGD RGNFMYSQWN GGEGQYGQCT MKVDFKDKIA EPPARARGAI ARTYFYMRDR YQLNLSRQQT QLFTAWNKQY PVTAWECERD ERIAKVQGNH NPYVQQACQA QKS
Validated NT Sequence
atcttactca cttagctctt ttgcgcctgg caagcctgct ggacatacgg attatggttg ccctgcactt tcgcgatgcg ctcatcgcgt tcacattccc aggcggtgac cgggtactgt ttattccagg cggtaaacag ctgagtttgc tggcgggaaa gattcagctg atagcggtcg cgcatataga agtaggtgcg ggcgatggcg ccgcgagcgc gggcaggggg ctcggcgatt ttatctttaa aatcgacctt catggtgcac tggccgtact ggccttcacc gccgttccac tgactgtaca taaagttgcc gcgatcgcca ttcacttctc ccaccgccgg ctgcaggttg tgcatgtcgc tttccatctt gcggtattcc ggatctttgg cgcagttttt acgtccaccc tcctgccagc actgacgctg atggccgaac tgccatgccg gcactacgtg ctcccattca acccggctgg cgcggttctc atttttacgc actttatagc cgcaggattc caaatcgata acgccttttt tcccctgcca gtcgatttta cagccgcaat aaaaatcgcc cggaacgtcg gcattcactt tgacgccagc ggctttggcc tgggaaaaac tattaatacc agcggccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHAA GINSFSQAKA AGVKVNADVP GDFYCGCKID WQGKKGVIDL ESCGYKVRKN ENRASRVEWE HVVPAWQFGH QRQCWQEGGR KNCAKDPEYR KMESDMHNLQ PAVGEVNGDR GNFMYSQWNG GEGQYGQCTM KVDFKDKIAE PPARARGAIA RTYFYMRDRY QLNLSRQQTQ LFTAWNKQYP VTAWECERDE RIAKVQGNHN PYVQQACQAQ KS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgctg gtattaatag tttttcccag gccaaagccg ctggcgtcaa agtgaatgcc gacgttccgg gcgattttta ttgcggctgt aaaatcgact ggcaggggaa aaaaggcgtt atcgatttgg aatcctgcgg ctataaagtg cgtaaaaatg agaaccgcgc cagccgggtt gaatgggagc acgtagtgcc ggcatggcag ttcggccatc agcgtcagtg ctggcaggag ggtggacgta aaaactgcgc caaagatccg gaataccgca agatggaaag cgatatgcac aacctgcagc cggcggtggg agaagtgaat ggcgatcgcg gcaactttat gtacagccag tggaacggcg gtgaaggcca gtacggccag tgcacaatga aggtcgattt taaagataaa atcgccgagc cccctgcccg cgctcgcggc gccatcgccc gcacctactt ctatatgcgc gaccgctatc agctgaatct ttcccgccag caaactcagc tgtttaccgc ctggaataaa cagtacccgg tcaccgcctg ggaatgtgaa cgtgatgagc gcatcgcgaa agtgcagggc aaccataatc cgtatgtcca gcaggcttgc caggcgcaaa agagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat