KlpnC.20494.a

Acetaldehyde dehydrogenase

CENTER ID: KlpnC.20494.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20494.a.B1.GE44453 Full length(KlpnC.20494.a) 1 316
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3020
UniProt: A0A0H3GR43

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRKRKVAIIG SGNIGTDLMI KILRHGQHLE MAVMVGIDPQ SDGLARARRL GVATTHEGVG GLMQMAEFAD IDFVFDATSA GAHIKNDAAL REAKPGIRVI DLTPAAIGPY CVPVVNLADN LHQGNVNMVT CGGQATIPMI AAVSRVAKVH YAEIVASIAS QSAGPGTRAN IDEFTETTSQ AIEKVGGAGK GKAIIVLNPA EPPLMMRDTV YVLSELASQE AIAASIVEMA AAVQAYVPGY RLKQQVQFEV IPEDRPVNLP GVGCFSGLKT AVYLEVEGAA HYLPAYAGNL DIMTSAALAT AEQMAGAMHS AAGATA
NT Sequence
ATGCGTAAAC GTAAAGTCGC CATTATCGGC TCCGGGAATA TCGGCACCGA TTTAATGATT AAAATCCTGC GCCACGGCCA GCATCTGGAG ATGGCGGTGA TGGTCGGCAT CGACCCGCAG TCGGATGGCC TGGCCCGCGC CCGGCGCCTG GGCGTTGCGA CCACTCACGA AGGGGTGGGC GGCCTGATGC AGATGGCGGA ATTTGCCGAC ATCGATTTTG TTTTTGACGC CACCAGCGCC GGGGCGCACA TCAAAAACGA TGCCGCGCTG CGCGAAGCGA AGCCGGGGAT CCGCGTTATC GACCTGACGC CGGCGGCGAT TGGCCCGTAC TGCGTACCGG TGGTCAACCT CGCGGACAAT CTCCATCAGG GCAACGTCAA TATGGTGACC TGCGGCGGGC AAGCGACCAT CCCGATGATC GCCGCGGTCT CGCGAGTGGC GAAGGTGCAT TACGCGGAGA TTGTGGCCTC GATCGCCAGT CAATCCGCCG GGCCGGGCAC GCGGGCCAAT ATTGACGAAT TCACCGAAAC CACCTCGCAG GCGATTGAAA AAGTCGGCGG GGCGGGCAAG GGCAAAGCGA TTATTGTACT GAACCCGGCG GAGCCGCCGC TGATGATGCG CGATACCGTC TACGTCCTGA GCGAGCTGGC GTCGCAGGAG GCCATTGCCG CCTCGATCGT CGAAATGGCG GCGGCAGTGC AGGCTTACGT CCCAGGCTAC CGGCTGAAGC AGCAGGTGCA GTTTGAGGTT ATCCCCGAGG ACAGGCCGGT CAACCTGCCC GGCGTCGGCT GTTTTTCCGG CCTGAAAACC GCGGTTTACC TCGAAGTGGA AGGGGCGGCG CACTATCTGC CAGCGTACGC GGGCAACCTC GATATTATGA CCTCGGCGGC GCTGGCGACG GCGGAGCAAA TGGCCGGGGC AATGCACAGC GCGGCGGGAG CGACAGCA
Details for KlpnC.20494.a.B1.GE44453
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMR KRKVAIIGSG NIGTDLMIKI LRHGQHLEMA VMVGIDPQSD GLARARRLGV ATTHEGVGGL MQMAEFADID FVFDATSAGA HIKNDAALRE AKPGIRVIDL TPAAIGPYCV PVVNLADNLH QGNVNMVTCG GQATIPMVAA VSRVAKVHYA EIVASIASQS AGPGTRANID EFTETTSQAI EKVGGAGKGK AIIVLNPAEP PLMMRDTVYI LSELASQEAI AASIAEMAAA VQAYVPGYRL KQQVQFEVIP EDRPVNLPGV GCFSGLKTAV YLEVEGAAHY LPAYAGNLDI MTSAALATAE QMAGAXHSAA GATA
Validated NT Sequence
tactcnctta tgctgtcgct cccgccgcgc tgtgnattgc cccggccatt tgctccgccg tcgccagcgc cgccgaggtc ataatatcga ggttgcccgc gtacgctggc agatagtgcg ccgccccttc cacttcgagg taaaccgcgg ttttcaggcc ggaaaaacag ccgacgccgg gcaggttgac cggcctgtcc tcggggataa cctcaaactg cacctgctgc ttcagccggt agcccgggac gtaagcctgc actgccgccg ccatttcggc gatcgaggcg gcaatcgcct cctgcgacgc cagctcgctc aggatgtaga cggtatcgcg catcatcagc ggcggctccg cagggttcag tacaataatc gctttgccct tgcccgcccc accgactttt tcaatcgcct gcgaggtggt ttcggtgaat tcgtcaatat tggcccgcgt gcccggcccg gcggattggc tggcgatcga ggccacaatc tccgcgtaat gcaccttcgc cactcgcgag accgcggcga ccatcgggat ggtcgcctgc ccgccgcagg tcaccatatt gacgttgccc tgatggagat tgtccgcgag gttgaccacc ggtacgcagt acgggccaat cgccgccggc gtcaggtcga taacgcggat ccccggcttc gcttcgcgca gcgcggcatc gtttttgatg tgcgccccgg cgctggtggc gtcaaaaaca aaatcgatgt cggcaaattc cgccatctgc atcaggccgc ccaccccttc gtgagtggtc gcaacgccca ggcgccgggc gcgggccagg ccatccgact gcgggtcgat gccgaccatc accgccatct ccagatgctg gccgtggcgc aggattttaa tcattaaatc ggtgccgata ttcccggagc cgataatggc gactttacgt ttacgcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMR KRKVAIIGSG NIGTDLMIKI LRHGQHLEMA VMVGIDPQSD GLARARRLGV ATTHEGVGGL MQMAEFADID FVFDATSAGA HIKNDAALRE AKPGIRVIDL TPAAIGPYCV PVVNLADNLH QGNVNMVTCG GQATIPMIAA VSRVAKVHYA EIVASIASQS AGPGTRANID EFTETTSQAI EKVGGAGKGK AIIVLNPAEP PLMMRDTVYV LSELASQEAI AASIVEMAAA VQAYVPGYRL KQQVQFEVIP EDRPVNLPGV GCFSGLKTAV YLEVEGAAHY LPAYAGNLDI MTSAALATAE QMAGAMHSAA GATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgta aacgtaaagt cgccattatc ggctccggga atatcggcac cgatttaatg attaaaatcc tgcgccacgg ccagcatctg gagatggcgg tgatggtcgg catcgacccg cagtcggatg gcctggcccg cgcccggcgc ctgggcgttg cgaccactca cgaaggggtg ggcggcctga tgcagatggc ggaatttgcc gacatcgatt ttgtttttga cgccaccagc gccggggcgc acatcaaaaa cgatgccgcg ctgcgcgaag cgaagccggg gatccgcgtt atcgacctga cgccggcggc gattggcccg tactgcgtac cggtggtcaa cctcgcggac aatctccatc agggcaacgt caatatggtg acctgcggcg ggcaagcgac catcccgatg atcgccgcgg tctcgcgagt ggcgaaggtg cattacgcgg agattgtggc ctcgatcgcc agtcaatccg ccgggccggg cacgcgggcc aatattgacg aattcaccga aaccacctcg caggcgattg aaaaagtcgg cggggcgggc aagggcaaag cgattattgt actgaacccg gcggagccgc cgctgatgat gcgcgatacc gtctacgtcc tgagcgagct ggcgtcgcag gaggccattg ccgcctcgat cgtcgaaatg gcggcggcag tgcaggctta cgtcccaggc taccggctga agcagcaggt gcagtttgag gttatccccg aggacaggcc ggtcaacctg cccggcgtcg gctgtttttc cggcctgaaa accgcggttt acctcgaagt ggaaggggcg gcgcactatc tgccagcgta cgcgggcaac ctcgatatta tgacctcggc ggcgctggcg acggcggagc aaatggccgg ggcaatgcac agcgcggcgg gagcgacagc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat