KlpnC.20493.a

TraT complement resistance protein

CENTER ID: KlpnC.20493.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20493.a.B2.GE44452 Mature protein(KlpnC.20493.a) 23 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.5368
UniProt: A0A0H3H5Q3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLNKFMAVM VVSSALVLSG CSAMGTAIKK RNLEVKTQMS ETIWLEPSNN KTVYLQIKNT SDKDMSGLQA KIASAVTSKG YQVVSNPDTA GYWIQANVLK ADKMDLRESQ GWLSRGYEGA VTGAALGAGI TAYNSSSAGA TLGVGLAAGL VGMAADAMVE DVNYTMITDV QIAERTKTQV QTDNVAVLRQ GTSGAKVQTS TETGNQHKYQ TRVVSNANKV NLKFPEAQPV LEDQLAKSIA NIL
NT Sequence
ATGAAGCTTA ATAAATTCAT GGCTGTGATG GTCGTCAGCT CTGCTCTGGT GTTGTCTGGA TGTAGTGCAA TGGGAACCGC TATTAAAAAG AGAAATCTGG AAGTAAAAAC CCAGATGAGC GAAACAATCT GGCTGGAACC TTCAAATAAT AAGACGGTTT ATCTGCAAAT CAAAAATACC TCCGATAAGG ATATGAGTGG CCTGCAGGCA AAAATTGCCA GCGCTGTCAC TTCAAAGGGA TATCAGGTCG TCAGTAACCC CGATACGGCG GGCTACTGGA TCCAGGCGAA TGTGCTCAAG GCCGATAAAA TGGACCTTCG TGAGTCTCAG GGCTGGTTAA GCCGGGGTTA TGAGGGCGCT GTGACTGGCG CCGCTCTTGG GGCGGGGATT ACCGCTTACA ACTCCAGTTC CGCGGGAGCA ACCCTGGGAG TAGGGCTTGC AGCCGGTCTG GTCGGAATGG CGGCTGACGC GATGGTGGAA GATGTGAACT ACACCATGAT CACCGATGTT CAGATCGCGG AAAGAACCAA AACACAGGTT CAGACTGATA ACGTGGCCGT ATTACGTCAG GGAACATCCG GAGCAAAAGT GCAGACAAGT ACTGAAACGG GTAATCAGCA TAAATACCAG ACTCGCGTCG TCTCTAACGC GAATAAAGTT AACCTCAAGT TCCCTGAAGC CCAGCCTGTT CTTGAAGACC AGCTGGCAAA ATCGATCGCT AATATCCTG
Details for KlpnC.20493.a.B2.GE44452
HARVESTED ON: data unavailable
SEQUENCED ON: 7/22/2021
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA MGTAIKKRNL EVKTQMSETI WLEPSNNKTV YLQIKNTSDK DMSGLQAKIA SAVTSKGYQV VSNPDTAGYW IQANVLKADK MDLRESQGWL SRGYEGAVTG AALGAGITAY NSSSAGATLG VGLAAGLVGM AADAMVEDVN YTMITDVQIA ERTKTQVQTD NVAVLRQGTS GAKVQTSTET GNQHKYQTRV VSNANKVNLK FPEAQPVLED QLAKSIANIL
Validated NT Sequence
atcttactca cttacaggat attagcgatc gattttgcca gctggtcttc aagaacaggc tgggcttcag ggaacttgag gttaacttta ttagcgttag agacgacgcg agtctggtat ttatgctgat tacccgtttc cgtacttgtc tgcacttttg cgccagatgt tccctgacgt aatacggcca cgttatcagt ctgaacctgt gttttggttc tttccgcgat ctgaacatcg gtgatcatgg tgtagttcac atcttccacc atcgcgtcag ccgccatccc caccagaccg gctgcaagcc ctacccccag ggtcgctccc gcagaactgg agttgtaagc ggtaatcccc gccccaagag cggcgccagt cactgccccc tcataacccc ggcttaacca accctgagat tcacgaaggt ccattttatc ggccttgagc acattagcct ggatccagta gcccgccgta tccgggttac tgacgacctg atatcccttt gaagtgacgg cgctggcaat ttttgcctgc aggccactca tatccttatc ggaggtattt ttgatttgca gataaaccgt cttattattt gaaggttcca gccagattgt ttcgctcatc tgggttttca cttccagatt tctcttttta atagcggttc ccattgccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHAM GTAIKKRNLE VKTQMSETIW LEPSNNKTVY LQIKNTSDKD MSGLQAKIAS AVTSKGYQVV SNPDTAGYWI QANVLKADKM DLRESQGWLS RGYEGAVTGA ALGAGITAYN SSSAGATLGV GLAAGLVGMA ADAMVEDVNY TMITDVQIAE RTKTQVQTDN VAVLRQGTSG AKVQTSTETG NQHKYQTRVV SNANKVNLKF PEAQPVLEDQ LAKSIANIL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcaatgg gaaccgctat taaaaagaga aatctggaag taaaaaccca gatgagcgaa acaatctggc tggaaccttc aaataataag acggtttatc tgcaaatcaa aaatacctcc gataaggata tgagtggcct gcaggcaaaa attgccagcg ctgtcacttc aaagggatat caggtcgtca gtaaccccga tacggcgggc tactggatcc aggcgaatgt gctcaaggcc gataaaatgg accttcgtga gtctcagggc tggttaagcc ggggttatga gggcgctgtg actggcgccg ctcttggggc ggggattacc gcttacaact ccagttccgc gggagcaacc ctgggagtag ggcttgcagc cggtctggtc ggaatggcgg ctgacgcgat ggtggaagat gtgaactaca ccatgatcac cgatgttcag atcgcggaaa gaaccaaaac acaggttcag actgataacg tggccgtatt acgtcaggga acatccggag caaaagtgca gacaagtact gaaacgggta atcagcataa ataccagact cgcgtcgtct ctaacgcgaa taaagttaac ctcaagttcc ctgaagccca gcctgttctt gaagaccagc tggcaaaatc gatcgctaat atcctgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t