SSGCID: Target Page

KlpnC.20488.a

Nicotinate phosphoribosyltransferase

CENTER ID:	KlpnC.20488.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20488.a.B1.GE44450	Full length(KlpnC.20488.a)		1	400

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20488.a.B1.PW39079	Full length(KlpnC.20488.a)		1	400

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.1798
UniProt:	A0A0H3GV33

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTQFTSPVLH SLLDTDAYKL HMQQAVFHRY GDVHVAAEFR CRGDDLLGIY ADAIREQVES MRDLRLRDDE YRWLSTLPFF RQDYLNWLRD FRYDPSQVTV SNDNGKLNIR LTGPWREAIM WEVPLLAVIS ELVHRYRSPE MGVDQALNTL EHKLGDFATM TADLDMSAFR LMDFGTRRRF SREVQEGIVR RLQQEPWFVG TSNYDLARRL NLTPMGTQAH EWFQAHQQIS PSLASSQRAA LAAWLEEYPD QLGIALTDCI TMDAFLRDFG PEFATRYQGL RHDSGDPVEW GEKAIAHYQK LGIDPLSKVL VFSDNLDLAK AVDLYRHFAS RVKLSFGIGT RLTCDLPQVK PLNIVIKLVE CNGKPVAKLS DSPGKTICHD KAFVRALREA FDLPPIKKAS

NT Sequence

ATGACACAAT TCACTTCTCC TGTACTGCAC TCGCTGCTTG ATACGGATGC CTATAAGCTG CACATGCAGC AGGCTGTCTT CCACCGCTAC GGCGATGTGC ATGTGGCGGC GGAGTTTCGC TGCCGCGGAG ACGACCTGCT CGGCATCTAT GCCGACGCGA TCCGCGAGCA GGTGGAGTCC ATGCGCGACC TGAGGCTGCG GGATGATGAA TATCGCTGGC TGTCCACCCT TCCCTTTTTT CGCCAGGATT ACCTCAACTG GCTGCGCGAT TTTCGCTATG ACCCGAGCCA GGTCACGGTC AGCAATGACA ATGGTAAGCT GAACATTCGC CTTACCGGCC CATGGCGGGA AGCCATCATG TGGGAAGTCC CGCTGCTGGC GGTGATAAGC GAGTTGGTCC ATCGCTATCG TTCACCAGAG ATGGGCGTCG ATCAGGCGCT CAACACGCTG GAACATAAGC TAGGCGACTT CGCCACCATG ACCGCTGACC TCGACATGAG CGCCTTCCGT CTGATGGACT TCGGCACCCG CCGTCGTTTC TCACGCGAGG TGCAGGAAGG TATTGTGCGG CGTCTGCAGC AGGAACCATG GTTTGTCGGC ACCAGCAATT ACGATCTGGC GCGCCGTCTG AATTTAACCC CGATGGGTAC CCAGGCCCAT GAGTGGTTCC AGGCCCACCA GCAGATCAGC CCCAGCCTTG CCAGCAGCCA GCGGGCGGCC CTTGCCGCCT GGCTGGAAGA GTATCCCGAC CAGCTGGGCA TCGCGCTGAC CGACTGCATC ACTATGGATG CGTTCCTGCG CGATTTCGGT CCGGAATTCG CCACCCGCTA TCAGGGGTTG CGCCACGATT CCGGCGACCC GGTGGAATGG GGAGAAAAAG CTATCGCCCA TTATCAGAAG CTGGGTATCG ATCCGCTGAG CAAAGTGCTG GTCTTCTCCG ACAATCTCGA CCTGGCGAAA GCCGTCGATC TGTATCGCCA CTTCGCCTCG CGGGTGAAGC TCAGCTTTGG GATCGGCACC CGTTTAACCT GCGATCTGCC GCAGGTTAAA CCGCTCAATA TCGTGATTAA GCTGGTGGAA TGTAACGGCA AGCCGGTGGC GAAACTCTCC GACAGCCCGG GGAAAACTAT CTGCCACGAT AAGGCCTTCG TCCGCGCCCT GCGCGAAGCG TTCGACCTGC CGCCGATTAA AAAGGCAAGC

Details for KlpnC.20488.a.B1.GE44450

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	47kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	89
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMT QFTSPVLHSL LDTDAYKLHM QQAVFHRYGD VHVAAEFRCR GDDLLGIYAD AIREQVESMR DLRLRDDEYR WLSTLPFFRQ DYLNWLRDFR YDPSQVTVSN DNGKLNIRLT GPWREAIMWE VPLLAVISEL VHRYRSPEMG VDQALNTLEH KLGDFATMTA DLDMSAFRLM DFGTRRRFSR EVXEGIVRRL QXEPWFVGTS NYDLXRRLNL TPMGTQAHXW XQAHQQISPS LASSQRXXLX AWLEEYPDQL GIALXXCITM DAFLRDFGPE XXXRYXGXXX DSXXPVEWGE KAIAHYQKXG IDPXSKVXVX XDNLXXAKXV XXYXXFASRX KXSFGXGTRL TCDXPQVXPL NIXIKLVECN GKPVAKLSDS PGKTXCHDKA XVRALREAXX

Validated NT Sequence

ggnnnaaagc ctcgcgcagg gcgcggacna aggccttatc gtggcanatg gttttccccg ggctgtcgga nagtttcgcc accggcttgc cgttacattc caccagctta atcncgatat tgagcggntt aacctgcggn anatcgcagg ttaaacgggt gccnatccca aagctganct tcncccgcga ggcgaanngn nnatacnnat nnacggnttt cgccnnnncn agattatcgn anaagaccan cactttgctc ancggatcga tncccnnctt ctgataatgg gcgatagctt tttctcccca ttccaccggg tnnncngaat cgnggnncan ccccngatag cgggnggnna nttccggacc gaaatcgcgc aggaacgcat ccatagtgat gcagtngntc agcgcgatgc ccagctggtc gggatactct tccagccagg cgncaaganc cncccgctgg ctgctggcaa ggctggggct gatctgctgg tgggcctgga nccantcatg ggcctgggta cccatcgggg ttaaattcag ncggcgcncc agatcgtaat tgctggtgcc gacaaaccat ggttcntgct gcagacgccg cacaatacct tcntgcacct cgcgtgagaa acgacggcgg gtgccgaagt ccatcagacg gaaggcgctc atgtcgaggt cagcggtcat ggtggcgaag tcgcctagct tatgttccag cgtgttgagc gcctgatcga cgcccatctc tggtgaacga tagcgatgga ccaactcgct tatcaccgcc agcagcggga cttcccacat gatggcttcc cgccatgggc cggtaaggcg aatgttcagc ttaccattgt cattgctgac cgtgacctgg ctcgggtcat agcgaaaatc gcgcagccag ttgaggtaat cctggcgaaa aaagggaagg gtggacagcc agcgatattc atcatcccgc agcctcaggt cgcgcatgga ctccacctgc tcgcggatcg cgtcggcata gatgccgagc aggtcgtctc cgcggcagcg aaactccgcc gccacatgca catcgccgta gcggtggaag acagcctgct gcatgtgcag cttataggca tccgtatcaa gcagcgagtg cagtacagga gaagtgaatt gtgtcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHMT QFTSPVLHSL LDTDAYKLHM QQAVFHRYGD VHVAAEFRCR GDDLLGIYAD AIREQVESMR DLRLRDDEYR WLSTLPFFRQ DYLNWLRDFR YDPSQVTVSN DNGKLNIRLT GPWREAIMWE VPLLAVISEL VHRYRSPEMG VDQALNTLEH KLGDFATMTA DLDMSAFRLM DFGTRRRFSR EVQEGIVRRL QQEPWFVGTS NYDLARRLNL TPMGTQAHEW FQAHQQISPS LASSQRAALA AWLEEYPDQL GIALTDCITM DAFLRDFGPE FATRYQGLRH DSGDPVEWGE KAIAHYQKLG IDPLSKVLVF SDNLDLAKAV DLYRHFASRV KLSFGIGTRL TCDLPQVKPL NIVIKLVECN GKPVAKLSDS PGKTICHDKA FVRALREAFD LPPIKKAS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac aattcacttc tcctgtactg cactcgctgc ttgatacgga tgcctataag ctgcacatgc agcaggctgt cttccaccgc tacggcgatg tgcatgtggc ggcggagttt cgctgccgcg gagacgacct gctcggcatc tatgccgacg cgatccgcga gcaggtggag tccatgcgcg acctgaggct gcgggatgat gaatatcgct ggctgtccac ccttcccttt tttcgccagg attacctcaa ctggctgcgc gattttcgct atgacccgag ccaggtcacg gtcagcaatg acaatggtaa gctgaacatt cgccttaccg gcccatggcg ggaagccatc atgtgggaag tcccgctgct ggcggtgata agcgagttgg tccatcgcta tcgttcacca gagatgggcg tcgatcaggc gctcaacacg ctggaacata agctaggcga cttcgccacc atgaccgctg acctcgacat gagcgccttc cgtctgatgg acttcggcac ccgccgtcgt ttctcacgcg aggtgcagga aggtattgtg cggcgtctgc agcaggaacc atggtttgtc ggcaccagca attacgatct ggcgcgccgt ctgaatttaa ccccgatggg tacccaggcc catgagtggt tccaggccca ccagcagatc agccccagcc ttgccagcag ccagcgggcg gcccttgccg cctggctgga agagtatccc gaccagctgg gcatcgcgct gaccgactgc atcactatgg atgcgttcct gcgcgatttc ggtccggaat tcgccacccg ctatcagggg ttgcgccacg attccggcga cccggtggaa tggggagaaa aagctatcgc ccattatcag aagctgggta tcgatccgct gagcaaagtg ctggtcttct ccgacaatct cgacctggcg aaagccgtcg atctgtatcg ccacttcgcc tcgcgggtga agctcagctt tgggatcggc acccgtttaa cctgcgatct gccgcaggtt aaaccgctca atatcgtgat taagctggtg gaatgtaacg gcaagccggt ggcgaaactc tccgacagcc cggggaaaac tatctgccac gataaggcct tcgtccgcgc cctgcgcgaa gcgttcgacc tgccgccgat taaaaaggca agctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for KlpnC.20488.a.B1.PW39079

PURIFICATION DATe:	8/10/2021
CONCENTRATION:	57mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	89
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMT QFTSPVLHSL LDTDAYKLHM QQAVFHRYGD VHVAAEFRCR GDDLLGIYAD AIREQVESMR DLRLRDDEYR WLSTLPFFRQ DYLNWLRDFR YDPSQVTVSN DNGKLNIRLT GPWREAIMWE VPLLAVISEL VHRYRSPEMG VDQALNTLEH KLGDFATMTA DLDMSAFRLM DFGTRRRFSR EVQEGIVRRL QQEPWFVGTS NYDLARRLNL TPMGTQAHEW FQAHQQISPS LASSQRAALA AWLEEYPDQL GIALTDCITM DAFLRDFGPE FATRYQGLRH DSGDPVEWGE KAIAHYQKLG IDPLSKVLVF SDNLDLAKAV DLYRHFASRV KLSFGIGTRL TCDLPQVKPL NIVIKLVECN GKPVAKLSDS PGKTICHDKA FVRALREAFD LPPIKKAS

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAC AATTCACTTC TCCTGTACTG CACTCGCTGC TTGATACGGA TGCCTATAAG CTGCACATGC AGCAGGCTGT CTTCCACCGC TACGGCGATG TGCATGTGGC GGCGGAGTTT CGCTGCCGCG GAGACGACCT GCTCGGCATC TATGCCGACG CGATCCGCGA GCAGGTGGAG TCCATGCGCG ACCTGAGGCT GCGGGATGAT GAATATCGCT GGCTGTCCAC CCTTCCCTTT TTTCGCCAGG ATTACCTCAA CTGGCTGCGC GATTTTCGCT ATGACCCGAG CCAGGTCACG GTCAGCAATG ACAATGGTAA GCTGAACATT CGCCTTACCG GCCCATGGCG GGAAGCCATC ATGTGGGAAG TCCCGCTGCT GGCGGTGATA AGCGAGTTGG TCCATCGCTA TCGTTCACCA GAGATGGGCG TCGATCAGGC GCTCAACACG CTGGAACATA AGCTAGGCGA CTTCGCCACC ATGACCGCTG ACCTCGACAT GAGCGCCTTC CGTCTGATGG ACTTCGGCAC CCGCCGTCGT TTCTCACGCG AGGTGCAGGA AGGTATTGTG CGGCGTCTGC AGCAGGAACC ATGGTTTGTC GGCACCAGCA ATTACGATCT GGCGCGCCGT CTGAATTTAA CCCCGATGGG TACCCAGGCC CATGAGTGGT TCCAGGCCCA CCAGCAGATC AGCCCCAGCC TTGCCAGCAG CCAGCGGGCG GCCCTTGCCG CCTGGCTGGA AGAGTATCCC GACCAGCTGG GCATCGCGCT GACCGACTGC ATCACTATGG ATGCGTTCCT GCGCGATTTC GGTCCGGAAT TCGCCACCCG CTATCAGGGG TTGCGCCACG ATTCCGGCGA CCCGGTGGAA TGGGGAGAAA AAGCTATCGC CCATTATCAG AAGCTGGGTA TCGATCCGCT GAGCAAAGTG CTGGTCTTCT CCGACAATCT CGACCTGGCG AAAGCCGTCG ATCTGTATCG CCACTTCGCC TCGCGGGTGA AGCTCAGCTT TGGGATCGGC ACCCGTTTAA CCTGCGATCT GCCGCAGGTT AAACCGCTCA ATATCGTGAT TAAGCTGGTG GAATGTAACG GCAAGCCGGT GGCGAAACTC TCCGACAGCC CGGGGAAAAC TATCTGCCAC GATAAGGCCT TCGTCCGCGC CCTGCGCGAA GCGTTCGACC TGCCGCCGAT TAAAAAGGCA AGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT