KlpnC.20485.a

Isochorismatase

CENTER ID: KlpnC.20485.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20485.a.B1.GE44447 Full length(KlpnC.20485.a) 1 283
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.1408
UniProt: A0A0H3GPR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAIPKLQAYA LPEASDIPAN KVNWAFEPSR AALLIHDMQE YFLNFWGENS AMMEKVVANI AALRDFCKQN GIPVYYTAQP KEQSDEDRAL LNDMWGPGLT RSPEQQQVIA ALAPDEDDTV LVKWRYSAFH RSPLEEMLKE TGRDQLIITG VYAHIGCMTT ATDAFMRDIK PFFVADALAD FSREEHLMAL KYVAGRSGRV VMTEELLPIP VSKAALRALI LPLLDESDEP LDDENLIDYG LDSVRMMALA ARWRKVHGDI DFVMLAKNPT IDAWWALLTR EVQ
NT Sequence
ATGGCAATCC CTAAATTACA GGCATATGCG CTGCCGGAAG CCAGCGATAT CCCGGCGAAC AAGGTCAACT GGGCCTTTGA GCCGTCGCGC GCCGCGCTGC TGATCCACGA CATGCAGGAG TATTTCCTCA ACTTCTGGGG CGAAAACAGC GCGATGATGG AGAAAGTGGT GGCTAATATC GCCGCCCTGC GCGACTTCTG CAAACAGAAC GGCATTCCGG TGTACTACAC CGCCCAGCCG AAAGAGCAGA GCGATGAAGA CCGCGCCCTG CTGAATGATA TGTGGGGGCC GGGTCTGACC CGCTCGCCGG AGCAGCAGCA GGTGATTGCC GCGCTGGCGC CGGATGAAGA CGATACCGTG CTGGTGAAAT GGCGCTACAG CGCGTTTCAT CGCTCGCCGC TGGAGGAGAT GCTGAAAGAG ACCGGCCGCG ATCAGCTGAT CATCACCGGC GTTTACGCCC ATATCGGCTG CATGACCACC GCCACCGATG CTTTCATGCG CGATATCAAA CCGTTCTTTG TCGCCGACGC GCTGGCAGAT TTCAGCCGTG AAGAGCACCT GATGGCGCTG AAATACGTCG CCGGCCGCTC CGGACGGGTG GTGATGACCG AAGAGCTGCT ACCGATCCCA GTCTCCAAAG CGGCGCTGCG CGCGCTGATC CTGCCGCTGC TCGACGAATC CGATGAACCG CTGGATGATG AAAACCTGAT CGACTACGGT CTGGATTCGG TGCGGATGAT GGCCCTGGCC GCCCGCTGGC GCAAAGTGCA CGGCGATATC GACTTCGTGA TGCTGGCGAA AAACCCGACC ATCGACGCCT GGTGGGCGCT GCTCACCCGC GAGGTGCAA
Details for KlpnC.20485.a.B1.GE44447
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA IPKLQAYALP EASDIPANKV NWAFEPSRAA LLIHDMQEYF LNFWGENSAM MEKVVANIAA LRDFCKQNGI PVYYTAQPKE QSDEDRALLN DMWGPGLTRS PEQQQVIAAL APDEDDTVLV KWRYSAFHRS PLEEMLKETG RDQLIITGVY AHIGCMTTAT DAFMRDIKPF FVADALADFS REEHLMALKY VAGRSGRVVM TEELLPLPAS KAALRALILP LLDESDEPLD DENLIDYGLD SVRMMALAAR WRKVHGDIDF VMLAKNPTID AWWALLTREV Q
Validated NT Sequence
atcttactca cttattgcac ctcgcgggtg agcagcgccc accaggcgtc gatggtcggg ttcttcgcca gcatcacgaa gtcgatatcg ccgtgcactt tgcgccagcg ggcggccagg gccatcatcc gcaccgaatc cagaccgtag tcgatcagat tttcatcatc cagcggttcg tcggattcgt cgagcagcgg caggatcagc gcgcgcagcg ccgctttgga ggccggcagc ggcagcagct cttcggtcat caccacgcgg ccggagcggc cagcgacgta tttcagcgcc atcaggtgct cttcacggct gaaatctgcc agcgcgtcgg cgacaaagaa cggtttgata tcgcgcatga aagcatcggt ggcggtggtc atgcagccga tatgggcgta aacgccggtg attatcagct ggtcgcgacc ggtctctttc agcatctcct ccagcggcga gcgatgaaac gcgctgtagc gccatttcac cagcacggta tcgtcttcat ccggcgccag cgcggcaatc acctgctgct gctccggcga gcgggtcaga cccggccccc acatatcatt cagcagggcg cggtcttcat cgctctgctc tttcggctgg gcggtgtagt acaccggaat gccgttctgt ttgcagaagt cgcgcagggc ggcgatatta gccaccactt tctccatcat cgcgctgttt tcgccccaga agttgaggaa atattcctgc atatcgtgga tcagcagcgc ggcgcgcgac ggctcaaagg cccagttgac cttgttcgcc gggatatcgc tggcttccgg cagcgcatat gcctgtaatt tagggattgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMA IPKLQAYALP EASDIPANKV NWAFEPSRAA LLIHDMQEYF LNFWGENSAM MEKVVANIAA LRDFCKQNGI PVYYTAQPKE QSDEDRALLN DMWGPGLTRS PEQQQVIAAL APDEDDTVLV KWRYSAFHRS PLEEMLKETG RDQLIITGVY AHIGCMTTAT DAFMRDIKPF FVADALADFS REEHLMALKY VAGRSGRVVM TEELLPIPVS KAALRALILP LLDESDEPLD DENLIDYGLD SVRMMALAAR WRKVHGDIDF VMLAKNPTID AWWALLTREV Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa tccctaaatt acaggcatat gcgctgccgg aagccagcga tatcccggcg aacaaggtca actgggcctt tgagccgtcg cgcgccgcgc tgctgatcca cgacatgcag gagtatttcc tcaacttctg gggcgaaaac agcgcgatga tggagaaagt ggtggctaat atcgccgccc tgcgcgactt ctgcaaacag aacggcattc cggtgtacta caccgcccag ccgaaagagc agagcgatga agaccgcgcc ctgctgaatg atatgtgggg gccgggtctg acccgctcgc cggagcagca gcaggtgatt gccgcgctgg cgccggatga agacgatacc gtgctggtga aatggcgcta cagcgcgttt catcgctcgc cgctggagga gatgctgaaa gagaccggcc gcgatcagct gatcatcacc ggcgtttacg cccatatcgg ctgcatgacc accgccaccg atgctttcat gcgcgatatc aaaccgttct ttgtcgccga cgcgctggca gatttcagcc gtgaagagca cctgatggcg ctgaaatacg tcgccggccg ctccggacgg gtggtgatga ccgaagagct gctaccgatc ccagtctcca aagcggcgct gcgcgcgctg atcctgccgc tgctcgacga atccgatgaa ccgctggatg atgaaaacct gatcgactac ggtctggatt cggtgcggat gatggccctg gccgcccgct ggcgcaaagt gcacggcgat atcgacttcg tgatgctggc gaaaaacccg accatcgacg cctggtgggc gctgctcacc cgcgaggtgc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat