KlpnC.20484.a

Quinolinate synthase A

CENTER ID: KlpnC.20484.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20484.a.B1.GE44446 Full length(KlpnC.20484.a) 1 348
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.1545
UniProt: A0A0H3GU73

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMSVMFDPET AIYPFPAKPQ PLTVDEKQFY REKIKRLLRE RDAVMVAHYY TDPEIQQLAE ETGGCIADSL EMARFGARHS ASTLLVAGVR FMGETAKILS PEKTILMPTL NAECSLDLGC PIEEFNAFCD AHPDRTVVVY ANTSAAVKAR ADWVVTSSIA VELIDHLDSL GQKILWAPDR HLGRYVQRQT GADVLCWQGA CIVHDEFKTQ ALMRMKALHP EAAVLVHPES PQAIVEMADA VGSTSQLIAA AKSLPQRQLI VATDRGIFYK MQQAVPEKTL LEAPTAGEGA TCRSCAHCPW MAMNGLKAIA EGLEQGGAEH EIHVDEALRT GALIPLNRML DFAATLRG
NT Sequence
ATGATGAGCG TAATGTTTGA TCCTGAAACG GCGATTTATC CTTTCCCTGC TAAACCGCAG CCGCTGACCG TCGACGAAAA GCAGTTTTAC CGCGAAAAAA TCAAGCGCCT GCTGCGCGAG CGCGATGCCG TGATGGTGGC GCATTACTAC ACCGATCCTG AAATTCAACA GCTGGCGGAA GAGACCGGCG GCTGTATCGC CGACTCGCTG GAGATGGCGC GCTTTGGCGC CCGCCATTCG GCCTCCACGC TGCTGGTCGC CGGAGTGCGT TTTATGGGGG AAACAGCCAA AATCCTCAGC CCGGAAAAGA CCATTTTGAT GCCGACCCTG AACGCCGAGT GTTCATTGGA TCTGGGCTGT CCGATTGAGG AATTCAACGC CTTTTGCGAC GCCCATCCTG ACCGCACCGT CGTGGTCTAT GCCAATACGT CCGCCGCGGT GAAGGCCCGC GCCGACTGGG TGGTGACCTC CAGCATCGCC GTGGAACTCA TTGACCATCT GGATAGTCTT GGTCAAAAGA TCCTCTGGGC GCCGGACCGC CACCTTGGGC GTTACGTTCA GCGTCAGACC GGCGCAGACG TGCTGTGCTG GCAGGGGGCG TGCATCGTGC ACGACGAGTT TAAAACCCAG GCGTTGATGC GGATGAAGGC TCTGCATCCC GAAGCCGCCG TGCTGGTCCA TCCCGAGTCG CCGCAGGCGA TCGTTGAGAT GGCCGATGCC GTAGGCTCCA CCAGCCAGCT GATTGCGGCG GCGAAAAGCC TGCCCCAGCG CCAGCTGATC GTGGCCACCG ATCGCGGTAT TTTCTATAAA ATGCAGCAGG CGGTGCCGGA GAAAACGCTG CTGGAAGCGC CCACCGCCGG CGAAGGAGCG ACCTGTCGCA GCTGCGCGCA TTGTCCGTGG ATGGCGATGA ATGGCCTGAA AGCCATTGCC GAGGGTCTCG AGCAGGGCGG CGCTGAACAT GAAATCCATG TCGACGAAGC GCTGCGAACC GGCGCATTAA TTCCCCTTAA CCGGATGCTG GATTTTGCGG CTACACTACG GGGA
Details for KlpnC.20484.a.B1.GE44446
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 77
Validated AA Sequence
MARFGARHSA STLLVAGVRF MGETAKILSP EKTILMPTLN AECSLDLGCP IEEFNAFCDA HPDRTVVVYA NTSAAVKARA DWVVTSSIAV ELIDHLDSLG QKILWAPDRH LGRYVQRQTG ADVLCWQGAC IVHDEFKTQA LMRMKALHPE AAVLVHPESP QAIVEMADAV GSTSQLIAAA KSLPQRQLIV ATDRGIFYKM QQAVPEKTLL EAPTAGEGAT CRSCAHCPWM AMNGLKAIAE GLEQGGAAHE IHVDEALRTG ALIPLNRMLD FAATLRG
Validated NT Sequence
tttcnnnntt tgttagcagc cggatcctat cttactcact tatccccgta gtgtagccgc aaaatccagc atccggttaa ggggaattaa tgcgccggtt cgcagcgctt cgtcgacatg gatttcatgt gcagcgccgc cctgctcgag accctcggca atggctttca ggccattcat cgccatccac ggacaatgcg cgcagctgcg acaggtcgct ccttcgccgg cggtgggcgc ttccagcagc gttttctccg gcaccgcctg ctgcatttta tagaaaatac cgcgatcggt ggccacgatc agctggcgct ggggcaggct tttcgccgcc gcaatcagct ggctggtgga gcctacggca tcggccatct caacgatcgc ctgcggcgac tcgggatgga ccagcacggc ggcttcggga tgcagagcct tcatccgcat caacgcctgg gttttaaact cgtcgtgcac gatgcacgcc ccctgccagc acagcacgtc tgcgccggtc tgacgctgaa cgtaacgccc aaggtggcgg tccggcgccc agaggatctt ttgaccaaga ctatccagat ggtcaatgag ttccacggcg atgctggagg tcaccaccca gtcggcgcgg gccttcaccg cggcggacgt attggcatag accacgacgg tgcggtcagg atgggcgtcg caaaaggcgt tgaattcctc aatcggacag cccagatcca atgaacactc ggcgttcagg gtcggcatca aaatggtctt ttccgggctg agaattttgg cggtttcccc cataaaacgc accccggcga ccagcagcgt ggaggccgaa tggcgggcgc caaagcgcgc catctccagc gagtcggcga tacagccgcc ggtctcttcc gccagctgtt gaatttcnna tcggtgtagt aatgcgccan catcanggca tcgcgctcgc gtagcagngc ttgatttttt cgcggtaaac tgcttttcgt cgacggtcag cggctgngnt tacaggaaag ganaaatcgc cntttcagga tcaaacnttn cnncnnntcn tngngnngnn nnnnag
Expected Protein Sequence
MAHHHHHHMM SVMFDPETAI YPFPAKPQPL TVDEKQFYRE KIKRLLRERD AVMVAHYYTD PEIQQLAEET GGCIADSLEM ARFGARHSAS TLLVAGVRFM GETAKILSPE KTILMPTLNA ECSLDLGCPI EEFNAFCDAH PDRTVVVYAN TSAAVKARAD WVVTSSIAVE LIDHLDSLGQ KILWAPDRHL GRYVQRQTGA DVLCWQGACI VHDEFKTQAL MRMKALHPEA AVLVHPESPQ AIVEMADAVG STSQLIAAAK SLPQRQLIVA TDRGIFYKMQ QAVPEKTLLE APTAGEGATC RSCAHCPWMA MNGLKAIAEG LEQGGAEHEI HVDEALRTGA LIPLNRMLDF AATLRG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatga gcgtaatgtt tgatcctgaa acggcgattt atcctttccc tgctaaaccg cagccgctga ccgtcgacga aaagcagttt taccgcgaaa aaatcaagcg cctgctgcgc gagcgcgatg ccgtgatggt ggcgcattac tacaccgatc ctgaaattca acagctggcg gaagagaccg gcggctgtat cgccgactcg ctggagatgg cgcgctttgg cgcccgccat tcggcctcca cgctgctggt cgccggagtg cgttttatgg gggaaacagc caaaatcctc agcccggaaa agaccatttt gatgccgacc ctgaacgccg agtgttcatt ggatctgggc tgtccgattg aggaattcaa cgccttttgc gacgcccatc ctgaccgcac cgtcgtggtc tatgccaata cgtccgccgc ggtgaaggcc cgcgccgact gggtggtgac ctccagcatc gccgtggaac tcattgacca tctggatagt cttggtcaaa agatcctctg ggcgccggac cgccaccttg ggcgttacgt tcagcgtcag accggcgcag acgtgctgtg ctggcagggg gcgtgcatcg tgcacgacga gtttaaaacc caggcgttga tgcggatgaa ggctctgcat cccgaagccg ccgtgctggt ccatcccgag tcgccgcagg cgatcgttga gatggccgat gccgtaggct ccaccagcca gctgattgcg gcggcgaaaa gcctgcccca gcgccagctg atcgtggcca ccgatcgcgg tattttctat aaaatgcagc aggcggtgcc ggagaaaacg ctgctggaag cgcccaccgc cggcgaagga gcgacctgtc gcagctgcgc gcattgtccg tggatggcga tgaatggcct gaaagccatt gccgagggtc tcgagcaggg cggcgctgaa catgaaatcc atgtcgacga agcgctgcga accggcgcat taattcccct taaccggatg ctggattttg cggctacact acggggatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at