SSGCID: Target Page

KlpnC.20478.a

Protoporphyrinogen oxidase

CENTER ID:	KlpnC.20478.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20478.a.B1.GE44441	Full length(KlpnC.20478.a)		1	177

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1125630.4.peg.187
UniProt:	A0A0H3GH88

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTLILFSTR DGQTREIASF LASELKELGI DADTLNLNRT DVVEWHHYDR VVIGASIRYG HFHPAVDRFV KKHLAALQAL PGAFFSVNLV ARKPEKRTPQ TNSYTRKFLL NSPWQPQSCA VFAGALRYPR YSWYDRFMIR LIMKMTGGET DTRKEVVYTD WQQVSRFARE IAQMARK

NT Sequence

GTGAAAACTT TAATTCTCTT CTCCACCCGC GACGGGCAGA CCCGTGAAAT CGCTTCGTTT CTCGCCTCTG AGCTTAAAGA GCTGGGCATC GATGCGGACA CCCTGAACCT GAACCGTACC GATGTCGTCG AATGGCATCA CTACGATCGG GTGGTCATTG GCGCGTCGAT TCGCTATGGG CACTTTCATC CGGCGGTGGA CCGCTTTGTG AAAAAGCATC TGGCAGCATT GCAGGCGCTG CCGGGGGCAT TCTTTTCGGT TAACCTGGTG GCGCGTAAAC CCGAGAAGCG CACGCCGCAG ACCAACAGCT ATACGCGTAA GTTTCTGCTG AACTCGCCGT GGCAGCCGCA GAGCTGCGCG GTATTTGCCG GCGCGCTGCG CTACCCGCGC TACAGCTGGT ACGATCGGTT TATGATCCGC CTGATTATGA AAATGACCGG GGGTGAAACG GATACCCGCA AAGAAGTGGT CTATACCGAC TGGCAACAGG TATCCCGGTT TGCTCGGGAA ATCGCACAAA TGGCGCGCAA A

Details for KlpnC.20478.a.B1.GE44441

HARVESTED ON:	data unavailable
SEQUENCED ON:	7/22/2021
EXPECTED MW:	22kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KTLILFSTRD GQTREIASFL ASELKELGID ADTLNLNRTD VVEWHHYDRV VIGASIRYGH FHPAVDRFVK KHLAALQALP GAFFSVNLVA RKPEKRTPQT NSYTRKFLLN SPWQPQSCAV FAGALRYPRY SWYDRFMIRL IMKMTGGETD TRKEVVYTDW QQVSRFAREI AQMARK

Validated NT Sequence

atcttactca cttatttgcg cgccatttgt gcgatttccc gagcaaaccg ggatacctgt tgccagtcgg tatagaccac ttctttgcgg gtatccgttt cacccccggt cattttcata atcaggcgga tcataaaccg atcgtaccag ctgtagcgcg ggtagcgcag cgcgccggca aataccgcgc agctctgcgg ctgccacggc gagttcagca gaaacttacg cgtatagctg ttggtctgcg gcgtgcgctt ctcgggttta cgcgccacca ggttaaccga aaagaatgcc cccggcagcg cctgcaatgc tgccagatgc tttttcacaa agcggtccac cgccggatga aagtgcccat agcgaatcga cgcgccaatg accacccgat cgtagtgatg ccattcgacg acatcggtac ggttcagatt cagggtgtcc gcatcgatgc ccagctcttt aagctcagag gcgagaaacg aagcgatttc acgggtctgc ccgtcgcggg tggagaagag aattaaagtt ttcaccatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMK TLILFSTRDG QTREIASFLA SELKELGIDA DTLNLNRTDV VEWHHYDRVV IGASIRYGHF HPAVDRFVKK HLAALQALPG AFFSVNLVAR KPEKRTPQTN SYTRKFLLNS PWQPQSCAVF AGALRYPRYS WYDRFMIRLI MKMTGGETDT RKEVVYTDWQ QVSRFAREIA QMARK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaaaa ctttaattct cttctccacc cgcgacgggc agacccgtga aatcgcttcg tttctcgcct ctgagcttaa agagctgggc atcgatgcgg acaccctgaa cctgaaccgt accgatgtcg tcgaatggca tcactacgat cgggtggtca ttggcgcgtc gattcgctat gggcactttc atccggcggt ggaccgcttt gtgaaaaagc atctggcagc attgcaggcg ctgccggggg cattcttttc ggttaacctg gtggcgcgta aacccgagaa gcgcacgccg cagaccaaca gctatacgcg taagtttctg ctgaactcgc cgtggcagcc gcagagctgc gcggtatttg ccggcgcgct gcgctacccg cgctacagct ggtacgatcg gtttatgatc cgcctgatta tgaaaatgac cgggggtgaa acggataccc gcaaagaagt ggtctatacc gactggcaac aggtatcccg gtttgctcgg gaaatcgcac aaatggcgcg caaatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat