SSGCID: Target Page

KlpnC.20474.a

AMP nucleosidase

CENTER ID:	KlpnC.20474.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20474.a.B1.GE44438	Full length(KlpnC.20474.a)		1	484

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20474.a.B1.PW39069	Full length(KlpnC.20474.a)		1	484

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.3414
UniProt:	A0A0H3GV47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNIKAASLTP EQALAELEAR YEASVTALRK AIGDYIDHNT LPDTEARAEG LFVYPQLSVS WDGADHKALK TRAWGRFTHA GCYTTTITNP KLFRNYLLEQ LTLLYQDYGA HISVELSQHE IPYPYVIDGS TLTLDRSMSA GLTRYFPTTE LSQIGDETAD GLFHPTEFYP LSHFDARRVD FSLARLRHYT GTPAEHFQPY VLFTNYTRYV DEFVSWGCSQ ILDPDSPYIA LSCAGGIWIT AETEAPEQAI SDLAWKKHQM PAWHLITHDG KGITLINIGV GPANAKTICD HLAVLRPDVW LMIGHCGGLR ESQAIGDYVL AHAYLRDDHV LDAVLPPDIP IPSIAEVQRA LYDATKQVSG MPGEEVKQRL RTGTVVTTDD RNWELRYSAS ALRFNLSRAV AIDMESATIA AQGYRFRVPY GTLLCVSDKP LHGEIKLPGQ ANRFYEGAIS EHLQIGIRAI DLLRAEGDHM HSRKLRTFNE PPFR

NT Sequence

ATGAATATCA AGGCCGCCAG TCTGACCCCG GAACAAGCGC TTGCCGAGCT GGAAGCGCGC TATGAAGCCT CGGTCACGGC GTTACGCAAA GCCATCGGCG ACTATATCGA CCATAATACG CTCCCCGATA CCGAAGCCCG CGCGGAAGGC CTTTTTGTCT ATCCGCAGCT GTCGGTCTCC TGGGACGGCG CCGATCATAA AGCCCTCAAA ACGCGCGCCT GGGGACGTTT CACCCATGCC GGTTGCTATA CCACCACCAT TACGAATCCG AAACTGTTCC GCAACTATTT GCTGGAACAG CTGACCCTGC TGTATCAGGA CTATGGCGCA CATATCAGCG TCGAGCTGTC GCAGCATGAG ATCCCCTATC CTTACGTGAT AGATGGTTCC ACCCTGACAC TCGACCGCTC GATGAGCGCC GGTCTGACGC GCTATTTCCC TACCACCGAG CTGTCACAAA TTGGCGATGA GACCGCGGAT GGCCTGTTCC ATCCCACCGA GTTCTATCCG CTGTCGCACT TTGACGCCCG CCGCGTCGAT TTTTCGCTGG CGCGTCTGCG TCACTACACC GGCACGCCGG CGGAACACTT CCAGCCCTAC GTGCTGTTCA CCAACTATAC CCGCTACGTG GATGAGTTCG TCAGCTGGGG CTGCAGCCAG ATCCTCGATC CCGACAGCCC CTATATCGCT CTCTCCTGCG CCGGCGGGAT CTGGATCACC GCCGAGACCG AAGCGCCGGA ACAGGCCATT TCCGATCTGG CGTGGAAGAA GCACCAGATG CCGGCCTGGC ATCTGATCAC CCACGACGGC AAAGGGATCA CCCTGATTAA CATTGGCGTC GGCCCGGCCA ATGCCAAAAC CATCTGCGAT CATCTCGCGG TACTGCGGCC GGACGTCTGG TTAATGATCG GCCACTGCGG CGGCCTGCGC GAAAGCCAGG CCATCGGCGA CTATGTGCTG GCCCACGCCT ATCTGCGTGA CGATCATGTA CTGGATGCGG TGCTGCCGCC AGATATTCCG ATCCCCAGCA TTGCCGAAGT GCAGCGCGCC CTCTACGACG CCACCAAGCA GGTGAGCGGG ATGCCCGGCG AAGAGGTTAA GCAGCGGCTG CGTACCGGGA CGGTGGTCAC CACCGACGAC CGTAACTGGG AGTTGCGCTA TTCCGCTTCG GCGCTGCGCT TTAATCTCAG CCGGGCGGTA GCTATCGATA TGGAGAGCGC GACCATCGCC GCCCAGGGCT ATCGCTTCCG CGTCCCCTAC GGCACGCTGC TCTGCGTCTC GGACAAGCCG CTGCACGGTG AAATCAAGCT CCCCGGCCAG GCTAACCGCT TTTACGAGGG CGCTATCTCC GAGCATCTGC AGATAGGCAT CCGGGCGATT GACCTGCTGC GCGCCGAGGG AGATCACATG CACTCGCGTA AGCTGCGCAC CTTCAACGAG CCGCCGTTCC GT

Details for KlpnC.20474.a.B1.GE44438

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	55kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMN IKAASLTPEQ ALAELEARYE ASVTALRKAI GDYIDHNTLP DTEARAEGLF VYPQLSVSWD GADHKALKTR AWGRFTHAGC YTTTITNPKL FRNYLLEQLT LLYQDYGAHI SVELSQHEIP YPYVIDGSTL TLDRSMSAGL TRYFPTTELS QIGDETADGL FHPTEFYPLS HFDARRVDFS LARLRHYTGT PAEHFQPYVL FTNYTRYVDE FVSWGCSQIL DPDSPYIALS CAGGIWITAE TEAPEQXISD LXWKKHQMPA WHLITHDGKG ITLINIGVGP ANAKTICDHL AVLRPDVWLM IGHCGGLRES QAIGDYVLAH AYLRDDHVLD AVLPPDIPIP SIAEVQRALY DATKQVSGMP GEEVKQRLRT GTVVTTDDRN WELRYSASAL RFNLSRAVAI DMESATIAAQ GYRFRVPYGT LLCVSDKPLH GEIKLPGQAN RFYEGAISEH LQIGIRAIDL LRAEGDHMHS RKL

Validated NT Sequence

gcgcagctta cgcgagtgca tgtgatctcc ctcggcgcgc agcaggtcaa tcgcccggat gcctatctgc agatgctcgg agatagcgcc ctcgtaaaag cggttagcct ggccggggag cttgatctca ccgtgcagcg gcttgtccga gacgcagagc agcgtgccgt aggggacgcg gaagcgatag ccctgggcgg cgatggtcgc gctctccata tcgatagcta ccgcgcggct gagattaaag cgcagcgccg aagcggaata gcgcagctcc cagttacggt cgtcggtggt gaccaccgtc ccggtacgca gccgctgctt aacctcttcg ccgggcatcc cgctcacctg cttggtggcg tcgtagaggg cgcgctgcac ttcggcaatg ctggggatcg gaatatctgg cggcagcacc gcatccagta catgatcgtc acgcagatag gcgtgggcca gcacatagtc gccgatggcc tggctttcgc gcaggccgcc gcagtggccg atcattaacc agacgtccgg ccgcagtacc gcgagatgat cgcagatggt tttggcgttg gccgggccga cgccaatgtt aatcagggtg atccctttgc cgtcgtgggt gatcagatgc caggccggca tctgatgctt cttccacncc agatcggaaa tggnctgttc cggcgcttcg gtctcggcgg tgatccagat cccgccggcg caggagagag cgatataggg gctgtcggga tcgaggatct ggctgcagcc ccagctgacg aactcatcca cgtagcgggt atagttggtg aacagcacgt agggctggaa gtgttccgcc ggcgtgccgg tgtagtgacg cagacgcgcc agcgaaaaat cgacgcggcg ggcgtcaaag tgcgacagcg gatagaactc ggtgggatgg aacaggccat ccgcggtctc atcgccaatt tgtgacagct cggtggtagg gaaatagcgc gtcagaccgg cgctcatcga gcggtcgagt gtcagggtgg aaccatcgat cacgtaagga taggggatct catgctgcga cagctcgacg ctgatatgcg cgccatagtc ctgatacagc agggtcagct gttccagcaa atagttgcgg aacagtttcg gattcgtaat ggtggtggta tagcaaccgg catgggtgaa acgtccccag gcgcgcgttt tgagggcttt atgatcggcg ccgtcccagg agaccgacag ctgcggatag acaaaaagac cttccgcgcg ggcttcggta tcggggagcg tattatggtc gatatagtcg ccgatggctt tgcgtaacgc cgtgaccgag gcttcatagc gcgcttccag ctcggcaagc gcttgttccg gggtcagact ggcggccttg atattcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

MAHHHHHHMN IKAASLTPEQ ALAELEARYE ASVTALRKAI GDYIDHNTLP DTEARAEGLF VYPQLSVSWD GADHKALKTR AWGRFTHAGC YTTTITNPKL FRNYLLEQLT LLYQDYGAHI SVELSQHEIP YPYVIDGSTL TLDRSMSAGL TRYFPTTELS QIGDETADGL FHPTEFYPLS HFDARRVDFS LARLRHYTGT PAEHFQPYVL FTNYTRYVDE FVSWGCSQIL DPDSPYIALS CAGGIWITAE TEAPEQAISD LAWKKHQMPA WHLITHDGKG ITLINIGVGP ANAKTICDHL AVLRPDVWLM IGHCGGLRES QAIGDYVLAH AYLRDDHVLD AVLPPDIPIP SIAEVQRALY DATKQVSGMP GEEVKQRLRT GTVVTTDDRN WELRYSASAL RFNLSRAVAI DMESATIAAQ GYRFRVPYGT LLCVSDKPLH GEIKLPGQAN RFYEGAISEH LQIGIRAIDL LRAEGDHMHS RKLRTFNEPP FR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata tcaaggccgc cagtctgacc ccggaacaag cgcttgccga gctggaagcg cgctatgaag cctcggtcac ggcgttacgc aaagccatcg gcgactatat cgaccataat acgctccccg ataccgaagc ccgcgcggaa ggcctttttg tctatccgca gctgtcggtc tcctgggacg gcgccgatca taaagccctc aaaacgcgcg cctggggacg tttcacccat gccggttgct ataccaccac cattacgaat ccgaaactgt tccgcaacta tttgctggaa cagctgaccc tgctgtatca ggactatggc gcacatatca gcgtcgagct gtcgcagcat gagatcccct atccttacgt gatagatggt tccaccctga cactcgaccg ctcgatgagc gccggtctga cgcgctattt ccctaccacc gagctgtcac aaattggcga tgagaccgcg gatggcctgt tccatcccac cgagttctat ccgctgtcgc actttgacgc ccgccgcgtc gatttttcgc tggcgcgtct gcgtcactac accggcacgc cggcggaaca cttccagccc tacgtgctgt tcaccaacta tacccgctac gtggatgagt tcgtcagctg gggctgcagc cagatcctcg atcccgacag cccctatatc gctctctcct gcgccggcgg gatctggatc accgccgaga ccgaagcgcc ggaacaggcc atttccgatc tggcgtggaa gaagcaccag atgccggcct ggcatctgat cacccacgac ggcaaaggga tcaccctgat taacattggc gtcggcccgg ccaatgccaa aaccatctgc gatcatctcg cggtactgcg gccggacgtc tggttaatga tcggccactg cggcggcctg cgcgaaagcc aggccatcgg cgactatgtg ctggcccacg cctatctgcg tgacgatcat gtactggatg cggtgctgcc gccagatatt ccgatcccca gcattgccga agtgcagcgc gccctctacg acgccaccaa gcaggtgagc gggatgcccg gcgaagaggt taagcagcgg ctgcgtaccg ggacggtggt caccaccgac gaccgtaact gggagttgcg ctattccgct tcggcgctgc gctttaatct cagccgggcg gtagctatcg atatggagag cgcgaccatc gccgcccagg gctatcgctt ccgcgtcccc tacggcacgc tgctctgcgt ctcggacaag ccgctgcacg gtgaaatcaa gctccccggc caggctaacc gcttttacga gggcgctatc tccgagcatc tgcagatagg catccgggcg attgacctgc tgcgcgccga gggagatcac atgcactcgc gtaagctgcg caccttcaac gagccgccgt tccgttgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for KlpnC.20474.a.B1.PW39069

PURIFICATION DATe:	7/29/2021
CONCENTRATION:	47mg/ml
OBSERVED MW:	55kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMN IKAASLTPEQ ALAELEARYE ASVTALRKAI GDYIDHNTLP DTEARAEGLF VYPQLSVSWD GADHKALKTR AWGRFTHAGC YTTTITNPKL FRNYLLEQLT LLYQDYGAHI SVELSQHEIP YPYVIDGSTL TLDRSMSAGL TRYFPTTELS QIGDETADGL FHPTEFYPLS HFDARRVDFS LARLRHYTGT PAEHFQPYVL FTNYTRYVDE FVSWGCSQIL DPDSPYIALS CAGGIWITAE TEAPEQAISD LAWKKHQMPA WHLITHDGKG ITLINIGVGP ANAKTICDHL AVLRPDVWLM IGHCGGLRES QAIGDYVLAH AYLRDDHVLD AVLPPDIPIP SIAEVQRALY DATKQVSGMP GEEVKQRLRT GTVVTTDDRN WELRYSASAL RFNLSRAVAI DMESATIAAQ GYRFRVPYGT LLCVSDKPLH GEIKLPGQAN RFYEGAISEH LQIGIRAIDL LRAEGDHMHS RKLRTFNEPP FR

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATA TCAAGGCCGC CAGTCTGACC CCGGAACAAG CGCTTGCCGA GCTGGAAGCG CGCTATGAAG CCTCGGTCAC GGCGTTACGC AAAGCCATCG GCGACTATAT CGACCATAAT ACGCTCCCCG ATACCGAAGC CCGCGCGGAA GGCCTTTTTG TCTATCCGCA GCTGTCGGTC TCCTGGGACG GCGCCGATCA TAAAGCCCTC AAAACGCGCG CCTGGGGACG TTTCACCCAT GCCGGTTGCT ATACCACCAC CATTACGAAT CCGAAACTGT TCCGCAACTA TTTGCTGGAA CAGCTGACCC TGCTGTATCA GGACTATGGC GCACATATCA GCGTCGAGCT GTCGCAGCAT GAGATCCCCT ATCCTTACGT GATAGATGGT TCCACCCTGA CACTCGACCG CTCGATGAGC GCCGGTCTGA CGCGCTATTT CCCTACCACC GAGCTGTCAC AAATTGGCGA TGAGACCGCG GATGGCCTGT TCCATCCCAC CGAGTTCTAT CCGCTGTCGC ACTTTGACGC CCGCCGCGTC GATTTTTCGC TGGCGCGTCT GCGTCACTAC ACCGGCACGC CGGCGGAACA CTTCCAGCCC TACGTGCTGT TCACCAACTA TACCCGCTAC GTGGATGAGT TCGTCAGCTG GGGCTGCAGC CAGATCCTCG ATCCCGACAG CCCCTATATC GCTCTCTCCT GCGCCGGCGG GATCTGGATC ACCGCCGAGA CCGAAGCGCC GGAACAGGCC ATTTCCGATC TGGCGTGGAA GAAGCACCAG ATGCCGGCCT GGCATCTGAT CACCCACGAC GGCAAAGGGA TCACCCTGAT TAACATTGGC GTCGGCCCGG CCAATGCCAA AACCATCTGC GATCATCTCG CGGTACTGCG GCCGGACGTC TGGTTAATGA TCGGCCACTG CGGCGGCCTG CGCGAAAGCC AGGCCATCGG CGACTATGTG CTGGCCCACG CCTATCTGCG TGACGATCAT GTACTGGATG CGGTGCTGCC GCCAGATATT CCGATCCCCA GCATTGCCGA AGTGCAGCGC GCCCTCTACG ACGCCACCAA GCAGGTGAGC GGGATGCCCG GCGAAGAGGT TAAGCAGCGG CTGCGTACCG GGACGGTGGT CACCACCGAC GACCGTAACT GGGAGTTGCG CTATTCCGCT TCGGCGCTGC GCTTTAATCT CAGCCGGGCG GTAGCTATCG ATATGGAGAG CGCGACCATC GCCGCCCAGG GCTATCGCTT CCGCGTCCCC TACGGCACGC TGCTCTGCGT CTCGGACAAG CCGCTGCACG GTGAAATCAA GCTCCCCGGC CAGGCTAACC GCTTTTACGA GGGCGCTATC TCCGAGCATC TGCAGATAGG CATCCGGGCG ATTGACCTGC TGCGCGCCGA GGGAGATCAC ATGCACTCGC GTAAGCTGCG CACCTTCAAC GAGCCGCCGT TCCGTTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT