SSGCID: Target Page

KlpnC.20470.a

Uncharacterized protein

CENTER ID:	KlpnC.20470.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20470.a.B1.GE44436	Full length(KlpnC.20470.a)		1	196

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
PATRIC ID:	fig\|1125630.4.peg.215
UniProt:	A0A0H3GKQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLQNPIHLRL EKLESWQHVT FMACLCERMY PNYAMFCKQT EFGDGLLYRR ILDLIWETLT VKDAKVNFDS QLEKLEEAIP AADDFDMYGV YPAIDACVAL SELIHSRLSG ETLEHAIEVS KTSITTVAML EMTQEGREMT DEELKANPAV EQEWDIQWEI FRLLADCEER DLELIKGLRA DLREAGESNI GINFQQ

NT Sequence

ATGTTACAAA ACCCGATTCA CCTGCGCCTG GAGAAGCTGG AAAGCTGGCA GCATGTCACT TTTATGGCCT GCCTGTGCGA ACGTATGTAT CCCAACTACG CGATGTTCTG CAAGCAAACC GAGTTTGGCG ACGGCCTGCT TTATCGTCGT ATCCTGGACC TGATCTGGGA AACGCTGACG GTTAAAGATG CGAAGGTGAA TTTCGACAGC CAGCTGGAGA AGCTGGAAGA GGCGATCCCG GCGGCAGATG ATTTCGACAT GTACGGTGTC TATCCGGCCA TAGACGCTTG CGTGGCGTTG AGTGAACTGA TTCATTCTCG TCTGAGCGGC GAAACGCTCG AGCACGCGAT CGAGGTGAGC AAGACCTCCA TCACCACCGT GGCGATGCTG GAAATGACCC AGGAAGGTCG CGAAATGACC GACGAAGAGT TGAAAGCGAA TCCGGCGGTA GAGCAGGAAT GGGATATTCA GTGGGAAATT TTCCGCCTGC TGGCCGACTG TGAAGAGCGT GATCTTGAGC TGATAAAAGG CCTGCGCGCG GACCTGCGCG AGGCTGGCGA GAGCAATATT GGTATAAATT TTCAGCAG

Details for KlpnC.20470.a.B1.GE44436

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	24kDa
OBSERVED MW:	24kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML QNPIHLRLEK LESWQHVTFM ACLCERMYPN YAMFCKQTEF GDGLLYRRIM DLIWETLTVK DAKVNFDSQL EKLEEAIPAA DDFDMYGVYP AIDACVALSE LIHSRLSGET LEHAIEVSKT SITTVAMLEM TQEGREMTDE ELKANPAVEQ EWDIQWEIFR LLADCEERDL ELIKGLRADL REAGESNIGI NFQQ

Validated NT Sequence

atcttactca cttactgctg aaaatttata ccaatattgc tctcgccagc ctcgcgcagg tccgcgcgca ggccttttat cagctcaaga tcacgctctt cacagtcggc cagcaggcgg aaaatttccc actgaatatc ccattcctgc tctaccgccg gattcgcttt caactcttcg tcggtcattt cgcgaccttc ctgggtcatt tccagcatcg ccacggtggt gatggaggtc ttgctcacct cgatcgcgtg ctcgagcgtt tcgccgctca gacgagaatg aatcagttca ctcaacgcca cgcaagcgtc tatggccgga tagacaccgt acatgtcgaa atcatctgcc gccgggatcg cctcttccag cttctccagc tggctgtcga aattcacctt cgcatcttta accgtcagcg tttcccagat caggtccatg atacgacgat aaagcaggcc gtcgccaaac tcggtttgct tgcagaacat cgcgtagttg ggatacatac gttcgcacag gcaggccata aaagtgacat gctgccagct ttccagcttc tccaggcgca ggtgaatcgg gttttgtaac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHML QNPIHLRLEK LESWQHVTFM ACLCERMYPN YAMFCKQTEF GDGLLYRRIL DLIWETLTVK DAKVNFDSQL EKLEEAIPAA DDFDMYGVYP AIDACVALSE LIHSRLSGET LEHAIEVSKT SITTVAMLEM TQEGREMTDE ELKANPAVEQ EWDIQWEIFR LLADCEERDL ELIKGLRADL REAGESNIGI NFQQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttac aaaacccgat tcacctgcgc ctggagaagc tggaaagctg gcagcatgtc acttttatgg cctgcctgtg cgaacgtatg tatcccaact acgcgatgtt ctgcaagcaa accgagtttg gcgacggcct gctttatcgt cgtatcctgg acctgatctg ggaaacgctg acggttaaag atgcgaaggt gaatttcgac agccagctgg agaagctgga agaggcgatc ccggcggcag atgatttcga catgtacggt gtctatccgg ccatagacgc ttgcgtggcg ttgagtgaac tgattcattc tcgtctgagc ggcgaaacgc tcgagcacgc gatcgaggtg agcaagacct ccatcaccac cgtggcgatg ctggaaatga cccaggaagg tcgcgaaatg accgacgaag agttgaaagc gaatccggcg gtagagcagg aatgggatat tcagtgggaa attttccgcc tgctggccga ctgtgaagag cgtgatcttg agctgataaa aggcctgcgc gcggacctgc gcgaggctgg cgagagcaat attggtataa attttcagca gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat