SSGCID: Target Page

KlpnC.20468.a

Polyamine aminopropyltransferase

CENTER ID:	KlpnC.20468.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20468.a.B1.GE44434	Full length(KlpnC.20468.a)		1	286

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20468.a.B1.PW39072	Full length(KlpnC.20468.a)		1	286

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.822
UniProt:	A0A0H3GMP3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MADNPLWHET LHDHFGQYFS VDNVLYHEKT DHQDLIIFDN RAFGRVMALD GVVQTTERDE FIYHEMMTHV PLLAHGNAKH VLIIGGGDGA MLREVSRHRS IETITMVEID AGVVSFCRQY LPNHNAGAYD DPRFTLVIDD GVNFVNQTTQ TFDVIISDCT DPIGPGESLF TSAFYEGCKR CLNPGGIFVA QNGVCFLQQD EAVGSHRKLS HYFRDVSFYQ AAIPTYYGGI MTFAWASDNE ALRHLSSEII QARFHKANLT CRYYNPAIHT AAFALPQYLH DALSAP

NT Sequence

GTGGCCGATA ATCCACTGTG GCATGAAACG CTGCATGACC ATTTTGGTCA GTATTTTAGC GTTGATAACG TGCTGTACCA CGAAAAAACC GATCATCAGG ATCTGATCAT CTTTGATAAC CGCGCCTTTG GCCGGGTGAT GGCGCTGGAT GGCGTCGTAC AAACCACCGA ACGCGACGAG TTCATCTATC ACGAAATGAT GACCCATGTG CCGCTGCTGG CTCACGGCAA CGCGAAGCAT GTGTTGATCA TTGGCGGCGG CGATGGCGCG ATGCTGCGCG AAGTCTCTCG TCACCGTAGC ATCGAAACCA TCACCATGGT GGAAATCGAC GCCGGGGTAG TTTCATTCTG CCGCCAGTAC CTGCCGAACC ATAACGCCGG CGCCTATGAC GATCCGCGCT TTACGCTGGT GATTGATGAT GGCGTCAATT TTGTTAATCA GACGACGCAA ACGTTTGACG TGATCATTTC GGACTGTACC GACCCTATCG GCCCGGGAGA AAGCCTGTTC ACCTCGGCTT TTTATGAAGG CTGCAAACGC TGCCTGAACC CGGGTGGGAT CTTTGTTGCC CAGAATGGCG TCTGCTTCCT GCAACAGGAT GAAGCGGTAG GCAGCCATCG CAAGCTAAGC CACTATTTTC GCGACGTCAG CTTCTACCAG GCGGCAATTC CAACCTATTA CGGCGGGATC ATGACCTTTG CCTGGGCGAG CGACAATGAG GCGCTGCGCC ATCTCTCCAG CGAAATCATT CAGGCGCGCT TTCACAAAGC CAACCTGACC TGCCGTTACT ACAATCCGGC AATCCATACG GCGGCCTTCG CCTTACCACA ATATCTGCAT GATGCTCTGT CCGCACCG

Details for KlpnC.20468.a.B1.GE44434

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV ADNPLWHETL HDHFGQYFSV DNVLYHEKTD HQDLIIFDNR AFGRVMALDG VVQTTERDEF IYHEMMTHVP LLAHGNAKHV LIIGGGDGAM LREVSRHRSI ETITMVEIDA GVVSFCRQYL PNHNAGAYDD PRFTLVIDDG VNFVNQTTQT FDVIISDCTD PIGPGESLFT SAFYEGCKRC LNPGGIFVAQ NGVCFLQQDE AVGSHRKLSH YFRDVSFYQA AIPTYYGGIM TFAWASDNEA LRHLSSEIIQ ARFHKANLTC RYYNPAIHTA AFALPQYLHD ALSAP

Validated NT Sequence

atcttactca cttacggtgc ggacagagca tcatgcagat attgtggtaa ggcgaaggcc gccgtatgga ttgccggatt gtagtaacgg caggtcaggt tggctttgtg aaagcgcgcc tgaatgattt cgctggagag atggcgcagc gcctcattgt cgctcgccca ggcaaaggtc atgatcccgc cgtaataggt tggaattgcc gcctggtaga agctgacgtc gcgaaaatag tggcttagct tgcgatggct gcctaccgct tcatcctgtt gcaggaagca gacgccattc tgggcaacaa agatcccacc cgggttcagg cagcgtttgc agccttcata aaaagccgag gtgaacaggc tttctcccgg gccgataggg tcggtacagt ccgaaatgat cacgtcaaac gtttgcgtcg tctgattaac aaaattgacg ccatcatcaa tcaccagcgt aaagcgcgga tcgtcatagg cgccggcgtt atggttcggc aggtactggc ggcagaatga aactaccccg gcgtcgattt ccaccatggt gatggtttcg atgctacggt gacgagagac ttcgcgcagc atcgcgccat cgccgccgcc aatgatcaac acatgcttcg cgttgccgtg agccagcagc ggcacatggg tcatcatttc gtgatagatg aactcgtcgc gttcggtggt ttgtacgacg ccatccagcg ccatcacccg gccaaaggcg cggttatcaa agatgatcag atcctgatga tcggtttttt cgtggtacag cacgttatca acgctaaaat actgaccaaa atggtcatgc agcgtttcat gccacagtgg attatcggcc accatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMA DNPLWHETLH DHFGQYFSVD NVLYHEKTDH QDLIIFDNRA FGRVMALDGV VQTTERDEFI YHEMMTHVPL LAHGNAKHVL IIGGGDGAML REVSRHRSIE TITMVEIDAG VVSFCRQYLP NHNAGAYDDP RFTLVIDDGV NFVNQTTQTF DVIISDCTDP IGPGESLFTS AFYEGCKRCL NPGGIFVAQN GVCFLQQDEA VGSHRKLSHY FRDVSFYQAA IPTYYGGIMT FAWASDNEAL RHLSSEIIQA RFHKANLTCR YYNPAIHTAA FALPQYLHDA LSAP

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggccg ataatccact gtggcatgaa acgctgcatg accattttgg tcagtatttt agcgttgata acgtgctgta ccacgaaaaa accgatcatc aggatctgat catctttgat aaccgcgcct ttggccgggt gatggcgctg gatggcgtcg tacaaaccac cgaacgcgac gagttcatct atcacgaaat gatgacccat gtgccgctgc tggctcacgg caacgcgaag catgtgttga tcattggcgg cggcgatggc gcgatgctgc gcgaagtctc tcgtcaccgt agcatcgaaa ccatcaccat ggtggaaatc gacgccgggg tagtttcatt ctgccgccag tacctgccga accataacgc cggcgcctat gacgatccgc gctttacgct ggtgattgat gatggcgtca attttgttaa tcagacgacg caaacgtttg acgtgatcat ttcggactgt accgacccta tcggcccggg agaaagcctg ttcacctcgg ctttttatga aggctgcaaa cgctgcctga acccgggtgg gatctttgtt gcccagaatg gcgtctgctt cctgcaacag gatgaagcgg taggcagcca tcgcaagcta agccactatt ttcgcgacgt cagcttctac caggcggcaa ttccaaccta ttacggcggg atcatgacct ttgcctgggc gagcgacaat gaggcgctgc gccatctctc cagcgaaatc attcaggcgc gctttcacaa agccaacctg acctgccgtt actacaatcc ggcaatccat acggcggcct tcgccttacc acaatatctg catgatgctc tgtccgcacc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for KlpnC.20468.a.B1.PW39072

PURIFICATION DATe:	7/30/2021
CONCENTRATION:	69mg/ml
OBSERVED MW:	33kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGGCCG ATAATCCACT GTGGCATGAA ACGCTGCATG ACCATTTTGG TCAGTATTTT AGCGTTGATA ACGTGCTGTA CCACGAAAAA ACCGATCATC AGGATCTGAT CATCTTTGAT AACCGCGCCT TTGGCCGGGT GATGGCGCTG GATGGCGTCG TACAAACCAC CGAACGCGAC GAGTTCATCT ATCACGAAAT GATGACCCAT GTGCCGCTGC TGGCTCACGG CAACGCGAAG CATGTGTTGA TCATTGGCGG CGGCGATGGC GCGATGCTGC GCGAAGTCTC TCGTCACCGT AGCATCGAAA CCATCACCAT GGTGGAAATC GACGCCGGGG TAGTTTCATT CTGCCGCCAG TACCTGCCGA ACCATAACGC CGGCGCCTAT GACGATCCGC GCTTTACGCT GGTGATTGAT GATGGCGTCA ATTTTGTTAA TCAGACGACG CAAACGTTTG ACGTGATCAT TTCGGACTGT ACCGACCCTA TCGGCCCGGG AGAAAGCCTG TTCACCTCGG CTTTTTATGA AGGCTGCAAA CGCTGCCTGA ACCCGGGTGG GATCTTTGTT GCCCAGAATG GCGTCTGCTT CCTGCAACAG GATGAAGCGG TAGGCAGCCA TCGCAAGCTA AGCCACTATT TTCGCGACGT CAGCTTCTAC CAGGCGGCAA TTCCAACCTA TTACGGCGGG ATCATGACCT TTGCCTGGGC GAGCGACAAT GAGGCGCTGC GCCATCTCTC CAGCGAAATC ATTCAGGCGC GCTTTCACAA AGCCAACCTG ACCTGCCGTT ACTACAATCC GGCAATCCAT ACGGCGGCCT TCGCCTTACC ACAATATCTG CATGATGCTC TGTCCGCACC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT