KlpnC.20467.a

Beta-D-glucoside glucohydrolase, periplasmic

CENTER ID: KlpnC.20467.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20467.a.B1.GE44433 Full length(KlpnC.20467.a) 1 755
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3539
UniProt: A0A0H3GQV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLALQPALA DELFGNHPLT PQARDAFVTD LLKKMTVDEK IGQLRLISVG PDNPKEAIRE MIKNGQVGAI FNTVTRPDIR VMQDQVMQLS RLKIPLFFAY DVLHGQRTVF PISLGLASSF NLDAVKTVGR VSAYEAADDG LNMTWAPMVD VSRDPRWGRA SEGFGEDTYL TTMMGQAMVE SMQGKSPADR YSVMTSVKHF AAYGAVEGGK EYNTVDMSPQ RLFNDYMPPY KAGLDAGSGA VMVALNSLNG TPATSDSWLL KDVLRDQWGF KGITVSDHGA IKELIKHGVA SDPEDAVRVA LKSGINMSMS DEYYSKYLPG LVKSGKVTMA ELDDATRHVL NVKYDMGLFN DPYSHLGPKD SDPQDTNAES RLHRKEAREV ARESLVLLKN RLDTLPLKKS GTIAVVGALA DSKRDMMGSW SAAGVADQSV TVLTGIKEAL GDNGKVIYAK GANVTDDKGI VDFLNLYENA VQVDPRSPQE MIDEAVAAAK QSDVVVAVVG EAQGMAHEAS SRTDITLPQS QRNLIAALKA TGKPLVLVLM NGRPLALVKE DQQADALLET WFAGTEGGHA IADVLFGVYN PSGKLPMSFP RSVGQIPTYY SHLNTGRPYN PEKPNKYTSR YFDEANGPLY PFGYGLSYTT FSVSDVNMSS ATMPRDGSVT ASVQVTNTGN REGATVTQLY LQDVTASMSR PVKMLRGFKK VTLKPGETQT VSFPIDVDAL KFWNQQMKYV AEPGKFNVFI GVDSARVKQS EFELL
NT Sequence
GTGAGTCTGG CGCTGCAACC CGCGCTGGCG GATGAACTGT TTGGTAACCA CCCCCTGACC CCGCAGGCGC GGGATGCCTT TGTCACCGAC CTGCTGAAAA AAATGACGGT GGACGAGAAA ATCGGCCAGC TGCGTCTGAT AAGCGTTGGC CCGGATAACC CGAAAGAGGC GATCCGTGAA ATGATCAAGA ACGGTCAGGT GGGGGCGATT TTTAACACCG TTACCCGCCC GGATATCCGT GTGATGCAGG ATCAGGTGAT GCAGCTTAGC CGCCTGAAGA TCCCGCTGTT CTTTGCCTAC GATGTGCTGC ACGGTCAGCG CACCGTGTTT CCGATAAGCC TCGGCCTGGC CTCGTCGTTT AACCTTGATG CGGTGAAGAC CGTGGGCCGC GTGTCAGCTT ATGAAGCCGC CGATGACGGC TTGAACATGA CCTGGGCGCC GATGGTCGAC GTCTCCCGCG ACCCGCGCTG GGGACGCGCT TCCGAAGGGT TCGGGGAAGA CACCTATCTG ACCACGATGA TGGGCCAGGC GATGGTGGAG TCGATGCAGG GCAAGAGCCC GGCGGACCGC TATTCGGTGA TGACCAGCGT CAAGCATTTC GCCGCCTACG GCGCGGTAGA GGGCGGGAAA GAGTACAACA CGGTCGACAT GAGCCCGCAG CGCCTGTTCA ACGACTATAT GCCGCCGTAC AAAGCCGGGC TTGATGCCGG CAGCGGCGCG GTGATGGTAG CCCTGAACTC GCTGAACGGC ACCCCGGCGA CCTCTGATTC CTGGCTGCTG AAAGACGTCC TGCGCGACCA GTGGGGCTTT AAAGGCATCA CCGTTTCCGA CCACGGCGCC ATCAAAGAGT TGATTAAGCA CGGCGTGGCC TCCGACCCGG AAGACGCGGT GCGCGTAGCG CTGAAGTCCG GCATCAACAT GAGCATGAGC GACGAGTATT ACAGTAAGTA CCTGCCGGGG CTGGTGAAGT CCGGCAAGGT GACCATGGCC GAGCTGGATG ATGCCACCCG ACACGTGCTC AACGTGAAAT ATGATATGGG ACTGTTCAAC GATCCCTACA GCCACCTGGG ACCGAAAGAT TCTGACCCGC AGGACACCAA TGCGGAAAGT CGTCTGCATC GTAAAGAGGC GCGCGAAGTG GCTCGCGAGA GCCTGGTGCT GCTGAAGAAC CGTCTTGATA CGCTGCCGCT GAAAAAATCC GGCACTATCG CGGTGGTGGG CGCGCTGGCG GACAGCAAGC GCGACATGAT GGGCAGCTGG TCCGCGGCCG GGGTGGCCGA CCAGTCGGTG ACCGTCCTGA CCGGCATCAA AGAAGCGCTG GGCGATAACG GCAAAGTGAT TTACGCCAAA GGGGCGAACG TTACCGATGA CAAAGGCATT GTCGATTTCC TCAATTTGTA TGAGAACGCG GTGCAGGTTG ACCCACGCTC GCCGCAGGAA ATGATCGACG AAGCGGTCGC GGCGGCGAAG CAATCCGATG TGGTGGTCGC CGTGGTGGGC GAGGCGCAGG GCATGGCGCA TGAGGCCTCC AGCCGGACCG ATATCACCCT GCCGCAGAGC CAGCGCAATC TGATTGCCGC TCTTAAAGCC ACCGGCAAAC CGCTGGTGCT GGTGCTGATG AACGGGCGTC CGCTGGCGCT GGTGAAAGAG GATCAGCAAG CCGATGCGCT GCTGGAGACC TGGTTTGCTG GCACCGAAGG CGGCCACGCC ATCGCCGACG TGCTGTTTGG CGTCTACAAC CCGTCGGGCA AGCTGCCGAT GTCTTTCCCG CGCTCGGTGG GGCAGATCCC AACCTACTAC AGCCACCTGA ATACCGGCCG GCCGTATAAT CCGGAGAAGC CGAACAAGTA CACCTCGCGT TACTTCGATG AAGCCAATGG CCCGCTGTAT CCGTTTGGTT ACGGCCTGAG CTACACCACC TTTAGCGTCT CCGATGTCAA CATGTCGTCG GCCACCATGC CGCGCGATGG CAGCGTGACC GCCAGCGTGC AGGTGACCAA CACCGGCAAT CGCGAAGGGG CGACGGTGAC CCAGCTCTAC CTGCAGGACG TCACCGCCTC CATGAGCCGG CCGGTGAAAA TGCTGCGCGG CTTTAAAAAG GTCACCCTCA AACCGGGCGA AACGCAAACC GTTAGCTTCC CAATCGACGT CGACGCGCTG AAGTTCTGGA ATCAGCAAAT GAAATACGTT GCTGAGCCGG GCAAATTCAA TGTCTTTATC GGCGTGGACT CAGCCCGCGT GAAGCAGAGC GAGTTTGAGC TGCTG
Details for KlpnC.20467.a.B1.GE44433
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 83kDa
OBSERVED MW: 83kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS LALQPALADE LFGNHPLTPQ ARDAFVTDLL KKMTVDEKIG QLRLISVGPD NPKEAIREMI KNGQVGAIFN TVTRPDIRVM QDQVMQLSRL KIPLFFAYDV LHGQRTVFPI SLGLASSFNL DAVKTVGRVS AYEAADDGLN MTWAPMVDVS RDPRWGRASE GFGEDTYLTT MMGQAMVESM QGKSPADRYS VMTSVKHFAA YGAVEGGKEY NTVDMSPQRL FNDYMPPYKA GLDAGSGAVM VALNSLNGTP ATSDSWLLKD VLRDQWGFKG ITVSDHGAIK ELIKHGVASD PEDAVRVALK SGINMSMSDE YYSKYLPGLV KSGKVTMAEL DDATRHVLNV KYDMGLFNDP YSHLGPKDSD PQDTNAESRL HRKEAREVAR ESLVLLKNRL DTLPLKKSGT IAVVGALADS KRDMMGSWSA AGVADQSVTV LTGIKEALGD NGKVIYAKGA NVTDDKGIVD FLNLYENAVQ VDPRSPQEMI DEAVAAAKQS DVVVAVVGEA QGMAHEASSR TDITLPQSQR NLIAALKATG KPLVLVLMNG RPLALVKEDQ QADALLETWF AGTEGGHAIA DVLFGVYNPS GKLPMSFPRS VGQIPTYYSH LNTGRPYNPE KPNKYTSRYF DEANGPLYPF GYGLSYTTFS VSDVNMSSAT MPRDGSVTAS VQVTNTGNRE GATVTQLYLQ DVTASMSRPV KMLRGFKKVT LKPGETQTVS FPIDVDALKF WNQQMKYVAE PGKFNVFIGV DSARVKQSEF ELL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgagtc tggcgctgca acccgcgctg gcggatgaac tgtttggtaa ccaccccctg accccgcagg cgcgggatgc ctttgtcacc gacctgctga aaaaaatgac ggtggacgag aaaatcggcc agctgcgtct gataagcgtt ggcccggata acccgaaaga ggcgatccgt gaaatgatca agaacggtca ggtgggggcg atttttaaca ccgttacccg cccggatatc cgtgtgatgc aggatcaggt gatgcagctt agccgcctga agatcccgct gttctttgcc tacgatgtgc tgcacggtca gcgcaccgtg tttccgataa gcctcggcct ggcctcgtcg tttaaccttg atgcggtgaa gaccgtgggc cgcgtgtcag cttatgaagc cgccgatgac ggcttgaaca tgacctgggc gccgatggtc gacgtctccc gcgacccgcg ctggggacgc gcttccgaag ggttcgggga agacacctat ctgaccacga tgatgggcca ggcgatggtg gagtcgatgc agggcaagag cccggcggac cgctattcgg tgatgaccag cgtcaagcat ttcgccgcct acggcgcggt agagggcggg aaagagtaca acacggtcga catgagcccg cagcgcctgt tcaacgacta tatgccgccg tacaaagccg ggcttgatgc cggcagcggc gcggtgatgg tagccctgaa ctcgctgaac ggcaccccgg cgacctctga ttcctggctg ctgaaagacg tcctgcgcga ccagtggggc tttaaaggca tcaccgtttc cgaccacggc gccatcaaag agttgattaa gcacggcgtg gcctccgacc cggaagacgc ggtgcgcgta gcgctgaagt ccggcatcaa catgagcatg agcgacgagt attacagtaa gtacctgccg gggctggtga agtccggcaa ggtgaccatg gccgagctgg atgatgccac ccgacacgtg ctcaacgtga aatatgatat gggactgttc aacgatccct acagccacct gggaccgaaa gattctgacc cgcaggacac caatgcggaa agtcgtctgc atcgtaaaga ggcgcgcgaa gtggctcgcg agagcctggt gctgctgaag aaccgtcttg atacgctgcc gctgaaaaaa tccggcacta tcgcggtggt gggcgcgctg gcggacagca agcgcgacat gatgggcagc tggtccgcgg ccggggtggc cgaccagtcg gtgaccgtcc tgaccggcat caaagaagcg ctgggcgata acggcaaagt gatttacgcc aaaggggcga acgttaccga tgacaaaggc attgtcgatt tcctcaattt gtatgagaac gcggtgcagg ttgacccacg ctcgccgcag gaaatgatcg acgaagcggt cgcggcggcg aagcaatccg atgtggtggt cgccgtggtg ggcgaggcgc agggcatggc gcatgaggcc tccagccgga ccgatatcac cctgccgcag agccagcgca atctgattgc cgctcttaaa gccaccggca aaccgctggt gctggtgctg atgaacgggc gtccgctggc gctggtgaaa gaggatcagc aagccgatgc gctgctggag acctggtttg ctggcaccga aggcggccac gccatcgccg acgtgctgtt tggcgtctac aacccgtcgg gcaagctgcc gatgtctttc ccgcgctcgg tggggcagat cccaacctac tacagccacc tgaataccgg ccggccgtat aatccggaga agccgaacaa gtacacctcg cgttacttcg atgaagccaa tggcccgctg tatccgtttg gttacggcct gagctacacc acctttagcg tctccgatgt caacatgtcg tcggccacca tgccgcgcga tggcagcgtg accgccagcg tgcaggtgac caacaccggc aatcgcgaag gggcgacggt gacccagctc tacctgcagg acgtcaccgc ctccatgagc cggccggtga aaatgctgcg cggctttaaa aaggtcaccc tcaaaccggg cgaaacgcaa accgttagct tcccaatcga cgtcgacgcg ctgaagttct ggaatcagca aatgaaatac gttgctgagc cgggcaaatt caatgtcttt atcggcgtgg actcagcccg cgtgaagcag agcgagtttg agctgctgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat