KlpnC.20463.a

Peptidoglycan lytic exotransglycosylase

CENTER ID: KlpnC.20463.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20463.a.B2.GE44430 Mature protein(KlpnC.20463.a) 27 645
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.680
UniProt: A0A0H3GIW3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEKAKKMTWH LLAASVGLLT LSQLAHADSL DEQRSRYAQI KQAWDNRQMD VVDQLMPTLS TYPLYPYLQY RQITDDLMNQ PALVVKNFID ANPTLPPARS LRSRFVNELA RRSDWRGLLA FSPDKPTSTE AQCNYYYAKL SVGQSQEAWS GAKELWLTGK NQPGACEPLF SAWRDSGQQD PLAYLERIRL AMKAGNIGLV KSLAQQMPAN YQSIASAVVA LANDPNSVLT FARTTGATDF TRQMAAVAFA SVARQDVENA RLMIPSLVQA QQLNEDQTQE LRDIVAWRLM GSDVTEEQAI WRDDAIMRSQ STPLVERRVR MALGTGDRHG LNTWLARLPM EAKEKDEWRY WQADLLLERG RDEEAQAILR SLMQQRGFYP MVAAQRLGEE YTFRIDKASG TIDPALASGP EMARVRELMY WNMDNTARTE WANLVTSRTK SQQAQLARYA FDQHWWDLSV QATIAGKLWD QLEERFPLAY NDLFARYVSG KDIPQSYAMA IARQESAWNP KVRSPVGASG LMQIMPGTAT HTVSMFSIPG YSGPSQLLDP ETNINIGTSY LQYVYQQFGN NRIYASAAYN AGPGRVRSWQ GNSAGRIDAV AFVESIPFSE TRGYVKNVLS YDAYYRYFMG QQDKILSDAE WRQRY
NT Sequence
GTGGAAAAAG CCAAAAAAAT GACGTGGCAT CTTTTGGCTG CCAGCGTTGG TTTGTTGACG TTGAGTCAGC TGGCGCATGC GGATTCACTG GATGAACAGC GCAGCCGCTA CGCACAAATT AAGCAGGCGT GGGATAACCG GCAGATGGAT GTAGTCGACC AGCTGATGCC CACGTTAAGC ACCTATCCCC TGTATCCCTA TTTGCAGTAC CGACAGATCA CCGACGATCT GATGAACCAG CCGGCGCTGG TGGTGAAAAA CTTTATTGAC GCCAATCCGA CGCTGCCCCC TGCGCGTTCA TTACGTTCGC GCTTCGTGAA TGAACTGGCC CGGCGCAGCG ACTGGCGCGG CTTGCTGGCG TTTAGCCCGG ATAAGCCGAC CAGCACCGAG GCGCAGTGCA ACTATTACTA CGCCAAGCTG AGCGTGGGAC AGTCGCAGGA GGCCTGGAGC GGGGCGAAAG AGCTGTGGCT GACGGGCAAG AATCAGCCCG GCGCCTGTGA ACCGCTGTTC AGCGCCTGGC GCGACTCCGG TCAGCAGGAT CCGCTGGCAT ATCTCGAACG GATCCGCCTG GCGATGAAGG CCGGCAATAT CGGGCTGGTG AAATCGCTGG CCCAGCAAAT GCCCGCCAAC TACCAGAGCA TCGCCAGCGC CGTGGTCGCC CTGGCTAACG ATCCAAACAG CGTCCTCACC TTCGCCCGCA CCACCGGGGC GACGGACTTT ACCCGTCAGA TGGCCGCCGT CGCCTTCGCC AGCGTCGCCC GCCAGGACGT GGAAAATGCC CGCCTGATGA TCCCTTCGCT GGTACAGGCT CAGCAGCTCA ATGAGGATCA GACCCAGGAA CTGCGCGATA TTGTCGCCTG GCGCCTGATG GGTAGCGATG TAACCGAAGA GCAGGCCATC TGGCGCGATG ACGCAATCAT GCGTTCGCAG TCCACGCCGC TGGTGGAGCG GCGGGTACGG ATGGCGTTGG GGACGGGCGA TCGCCATGGC CTCAACACCT GGCTGGCGCG CCTGCCGATG GAGGCGAAAG AGAAAGACGA ATGGCGCTAC TGGCAGGCGG ATCTGCTGCT GGAGCGCGGC AGGGATGAGG AGGCGCAGGC GATCCTTCGC TCGTTGATGC AACAGCGAGG CTTCTACCCG ATGGTCGCCG CGCAGCGTCT TGGCGAGGAG TATACCTTCC GCATCGATAA AGCCTCGGGC ACCATCGATC CGGCGCTGGC CTCCGGGCCG GAGATGGCGC GGGTACGTGA GCTGATGTAC TGGAATATGG ATAACACCGC GCGCACCGAA TGGGCCAACC TGGTCACTAG TCGCACCAAA TCCCAGCAGG CGCAGCTGGC GCGCTATGCC TTCGATCAGC ACTGGTGGGA TCTGAGCGTG CAGGCGACGA TCGCCGGTAA GCTGTGGGAT CAGCTGGAAG AACGTTTCCC GCTGGCCTAT AACGACCTGT TTGCCCGCTA TGTTAGCGGC AAAGATATTC CGCAGAGCTA CGCGATGGCG ATTGCCCGTC AGGAGAGCGC CTGGAACCCG AAAGTGCGCT CGCCGGTCGG CGCCAGCGGA CTGATGCAGA TTATGCCGGG CACCGCGACG CATACCGTCA GTATGTTCAG CATTCCCGGC TACAGCGGCC CGTCGCAGCT GTTGGATCCG GAAACCAACA TCAACATCGG CACCAGCTAT CTGCAGTACG TTTATCAGCA GTTCGGCAAT AACCGTATCT ACGCCTCAGC GGCCTATAAC GCCGGGCCGG GCCGGGTACG CAGCTGGCAG GGGAACAGCG CCGGACGGAT CGACGCCGTG GCGTTTGTCG AGAGTATTCC GTTTTCGGAA ACCCGCGGCT ACGTGAAGAA TGTCCTCTCC TATGACGCCT ACTACCGCTA CTTTATGGGG CAGCAGGATA AGATCCTCAG CGATGCGGAG TGGCGCCAGC GTTAC
Details for KlpnC.20463.a.B2.GE44430
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 71kDa
OBSERVED MW: 71kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHAD SLDEQRSRYA QIKQAWDNRQ MDVVDQLMPT LSTYPLYPYL QYRQITDDLM NQPALVVKNF IDANPTLPPA RSLRSRFVNE LARRSDWRGL LAFSPDKPTS TEAQCNYYYA KLSVGQSQEA WSGAKELWLT GKNQPGACEP LFSAWRDSGQ QDPLAYLERI RLAMKAGNIG LVKSLAQQMP ANYQSIASAV VALANDPNSV LTFARTTGAT DFTRQMAAVA FASVARQDVE NARLMIPSLV QAQQLNEDQT QELRDIVAWR LMGSDVTEEQ AIWRDDAIMR SQSTPLVERR VRMALGTGDR HGLNTWLARL PMEAKEKDEW RYWQADLLLE RGRDEEAQAI LRSLMQQRGF YPMVAAQRLG EEYTFRIDKA SGTIDPALAS GPEMARVREL MYWNMDNTAR TEWANLVTSR TKSQQAQLAR YAFDQHWWDL SVQATIAGKL WDQLEERFPL AYNDLFARYV SGKDIPQSYA MAIARQESAW NPKVRSPVGA SGLMQIMPGT ATHTVSMFSI PGYSGPSQLL DPETNINIGT SYLQYVYQQF GNNRIYASAA YNAGPGRVRS WQGNSAGRID AVAFVESIPF SETRGYVKNV LSYDAYYRYF MGQQDKILSD AEWRQRY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcggatt cactggatga acagcgcagc cgctacgcac aaattaagca ggcgtgggat aaccggcaga tggatgtagt cgaccagctg atgcccacgt taagcaccta tcccctgtat ccctatttgc agtaccgaca gatcaccgac gatctgatga accagccggc gctggtggtg aaaaacttta ttgacgccaa tccgacgctg ccccctgcgc gttcattacg ttcgcgcttc gtgaatgaac tggcccggcg cagcgactgg cgcggcttgc tggcgtttag cccggataag ccgaccagca ccgaggcgca gtgcaactat tactacgcca agctgagcgt gggacagtcg caggaggcct ggagcggggc gaaagagctg tggctgacgg gcaagaatca gcccggcgcc tgtgaaccgc tgttcagcgc ctggcgcgac tccggtcagc aggatccgct ggcatatctc gaacggatcc gcctggcgat gaaggccggc aatatcgggc tggtgaaatc gctggcccag caaatgcccg ccaactacca gagcatcgcc agcgccgtgg tcgccctggc taacgatcca aacagcgtcc tcaccttcgc ccgcaccacc ggggcgacgg actttacccg tcagatggcc gccgtcgcct tcgccagcgt cgcccgccag gacgtggaaa atgcccgcct gatgatccct tcgctggtac aggctcagca gctcaatgag gatcagaccc aggaactgcg cgatattgtc gcctggcgcc tgatgggtag cgatgtaacc gaagagcagg ccatctggcg cgatgacgca atcatgcgtt cgcagtccac gccgctggtg gagcggcggg tacggatggc gttggggacg ggcgatcgcc atggcctcaa cacctggctg gcgcgcctgc cgatggaggc gaaagagaaa gacgaatggc gctactggca ggcggatctg ctgctggagc gcggcaggga tgaggaggcg caggcgatcc ttcgctcgtt gatgcaacag cgaggcttct acccgatggt cgccgcgcag cgtcttggcg aggagtatac cttccgcatc gataaagcct cgggcaccat cgatccggcg ctggcctccg ggccggagat ggcgcgggta cgtgagctga tgtactggaa tatggataac accgcgcgca ccgaatgggc caacctggtc actagtcgca ccaaatccca gcaggcgcag ctggcgcgct atgccttcga tcagcactgg tgggatctga gcgtgcaggc gacgatcgcc ggtaagctgt gggatcagct ggaagaacgt ttcccgctgg cctataacga cctgtttgcc cgctatgtta gcggcaaaga tattccgcag agctacgcga tggcgattgc ccgtcaggag agcgcctgga acccgaaagt gcgctcgccg gtcggcgcca gcggactgat gcagattatg ccgggcaccg cgacgcatac cgtcagtatg ttcagcattc ccggctacag cggcccgtcg cagctgttgg atccggaaac caacatcaac atcggcacca gctatctgca gtacgtttat cagcagttcg gcaataaccg tatctacgcc tcagcggcct ataacgccgg gccgggccgg gtacgcagct ggcaggggaa cagcgccgga cggatcgacg ccgtggcgtt tgtcgagagt attccgtttt cggaaacccg cggctacgtg aagaatgtcc tctcctatga cgcctactac cgctacttta tggggcagca ggataagatc ctcagcgatg cggagtggcg ccagcgttac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat