KlpnC.20460.a

Deoxyguanosinetriphosphate triphosphohydrolase

CENTER ID: KlpnC.20460.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20460.a.B1.GE44427 Full length(KlpnC.20460.a) 1 504
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20460.a.B1.PW39076 Full length(KlpnC.20460.a) 1 504

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.881
UniProt: A0A0H3GNI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKIDFRNKI NWRRRFRSPP RVETERDILR IFESDRGRIV NSPAIRRLQQ KTQVFPLERN AAVRTRLTHS LEVQQVGRYI AKEVLSRLKE QRLLEEYGLE ELTGPFESVV EMACLMHDIG NPPFGHFGEA AINDWFRQRL APGDALGQPL IDDRCEVQAL RLHDGETSLN ALRRKVRQDL CSFEGNAQGI RLVHTLMRMN LTWAQVGCIL KYTRPAWWSE ETPASHSYLM KKPGYYLAEE EYVARLRKEL DLAPYNRFPL TWIMEAADDI SYCVADLEDA VEKRIFSADQ LYQHLYDAWG SHEKGSLFSQ VVENAWEKSR ANYLKQSAED QFFMYLRVNT LNKLVPYAAR RFIDNLPAIF TGDFNHALLE DDSDCSQLLE LYKNVAMKQV FSHPDVEQLE LQGYRVISGL LDIYQPLLKL SLEDFSELVA QERVRRLPIA SRLYQKLSTR HRLAYVEAVN KLARTAPEFA LMEYYYRCRL IQDYISGMTD LYAWDEYRRL MAVE
NT Sequence
ATGGCAAAGA TTGATTTTCG CAACAAGATA AACTGGCGTC GACGCTTCCG TTCGCCGCCG CGGGTGGAGA CGGAGCGCGA CATCCTGCGG ATCTTTGAAA GCGATCGGGG ACGGATCGTC AACTCGCCGG CAATCCGCCG CCTGCAGCAA AAAACTCAGG TCTTCCCGCT CGAGCGCAAC GCGGCGGTGC GCACTCGCCT GACCCACTCG CTGGAGGTGC AGCAGGTGGG GCGCTATATC GCCAAAGAGG TGCTGAGCCG GCTGAAAGAG CAGAGGCTGC TGGAAGAGTA TGGCCTGGAA GAGCTGACCG GGCCATTCGA GAGCGTGGTG GAGATGGCCT GCCTGATGCA CGATATCGGC AATCCCCCGT TTGGCCATTT TGGCGAAGCG GCGATTAACG ACTGGTTCCG TCAGCGCCTG GCGCCCGGCG ATGCGCTCGG GCAGCCGCTG ATCGACGATC GCTGCGAAGT GCAGGCCTTA CGCCTGCATG ACGGAGAGAC ATCGCTTAAT GCGCTGCGCC GTAAAGTGCG CCAGGATCTC TGCAGCTTTG AGGGCAACGC GCAGGGCATT CGTCTGGTCC ATACGCTAAT GCGTATGAAC CTGACCTGGG CGCAGGTGGG ATGTATCCTG AAATATACTC GTCCGGCGTG GTGGTCTGAG GAGACGCCGG CCAGCCACAG TTATTTAATG AAGAAACCTG GCTATTATTT AGCCGAAGAA GAGTATGTCG CGCGTTTGCG TAAAGAACTG GATCTCGCGC CTTACAATCG GTTTCCATTA ACCTGGATTA TGGAGGCTGC GGATGACATC TCATATTGCG TCGCGGATCT CGAGGACGCG GTGGAAAAAC GTATTTTTAG CGCAGACCAG CTCTATCAGC ATCTTTACGA CGCCTGGGGC AGTCATGAAA AAGGCTCGTT ATTTTCGCAG GTAGTAGAAA ATGCCTGGGA GAAATCGCGA GCCAATTATC TGAAGCAGAG CGCGGAAGAT CAGTTTTTTA TGTATTTGCG GGTGAATACG CTGAATAAGC TGGTGCCCTA CGCGGCGCGC CGATTTATTG ACAACCTGCC GGCAATCTTC ACGGGGGACT TTAATCATGC GTTGCTGGAG GACGACAGCG ATTGCAGTCA GCTACTGGAG CTCTATAAAA ATGTGGCGAT GAAACAGGTC TTTAGCCATC CGGATGTTGA GCAGCTGGAA TTGCAGGGCT ACCGGGTGAT CAGCGGCCTG TTGGATATCT ATCAGCCGCT GCTGAAATTA TCGCTGGAGG ATTTCAGCGA ATTAGTAGCG CAGGAGCGGG TACGACGTTT ACCCATTGCC TCCCGGCTGT ATCAGAAACT TTCGACCCGT CACCGGCTGG CCTATGTTGA AGCGGTAAAT AAACTGGCGC GTACGGCGCC CGAATTTGCG CTGATGGAAT ACTATTATCG CTGCCGTCTG ATTCAGGACT ATATCAGCGG GATGACGGAT TTGTATGCGT GGGATGAATA TCGGCGACTC ATGGCGGTGG AA
Details for KlpnC.20460.a.B1.GE44427
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMA KIDFRNKINW RRRFRSPPRV ETERDILRIF ESDRGRIVNS PAIRRLQQKT QVFPLERNAA VRTRLTHSLE VQQVGRYIAK EVLSRLKELR LLEEYGLEEL TGPFESVVEM ACLMHDIGNP PFGHFGEAAI NDWFRQRLAP GDALGQPLTD DRCEVQALRL HDGETSLNAL RRKVRQDLCS FXGNAXGIRL VHTLMRMNLT WAQVGCILKY TRPAWWSEET PASHSYLMKK PGYYLAEEEY VARLRKELDL APYNRFPLTW IMEAADDISY CVADLEDAVE KRIFSAEQLY QHLYDAWGSH EKGSLFSQVV ENAWEKSRAN YLKQSAEDQF FMYLRVNTLN KLVPYAARRF IDNLPAIFTG DFNHALLEDD SDCSQLLELY KNVAMKQVFS HPDVEQLELQ GYRVISGLLD IYQPLLKLSL EDFSELVAQE RVRRLPIASR LYQKLSTRHR LAYVEAVNKL ARTAPEFALM EYYYRCRLIQ DYISGMTDLY AWDE
Validated NT Sequence
attcatccca cgcatacaaa tccgtcatcc cgctgatata gtcctgaatc agacggcagc gataatagta ttccatcagc gcaaattcgg gcgccgtacg cgccagttta tttaccgctt caacataggc cagccggtga cgggtcgaaa gtttctgata cagccgggag gcaatgggta aacgtcgtac ccgctcctgc gctactaatt cgctgaaatc ctccagcgat aatttcagca gcggctgata gatatccaac aggccgctga tcacccggta gccctgcaat tccagctgct caacatccgg atggctaaag acctgtttca tcgccacatt tttatagagc tccagtagct gactgcaatc gctgtcgtcc tccagcaacg catgattaaa gtcccccgtg aagattgccg gcaggttgtc aataaatcgg cgcgccgcgt agggcaccag cttattcagc gtattcaccc gcaaatacat aaaaaactga tcttccgcgc tctgcttcag ataattggct cgcgatttct cccaggcatt ttctactacc tgcgaaaata acgagccttt ttcatgactg ccccaggcgt cgtaaagatg ctgatagagc tgttctgcgc taaaaatacg tttttccacc gcgtcctcga gatccgcgac gcaatatgag atgtcatccg cagcctccat aatccaggtt aatggaaacc gattgtaagg cgcgagatcc agttctttac gcaaacgcgc gacatactct tcttcggcta aataatagcc aggtttcttc attaaataac tgtggctggc cggcgtctcc tcagaccacc acgccggacg agtatatttc aggatacatc ccacctgcgc ccaggtcagg ttcatacgca ttagcgtatg gaccagacga atgccntgcg cgttgccntc aaagctgcag agatcctggc gcactttacg gcgcagcgca ttaagcgatg tctctccgtc atgcaggcgt aaggcctgca cttcgcagcg atcgtcggtc agcggctgcc cgagcgcatc gccgggcgcc aggcgctgac ggaaccagtc gttaatcgcc gcttcgccaa aatggccaaa cgggggattg ccgatatcgt gcatcaggca ggccatctcc accacgctct cgaatggccc ggtcagctct tccaggccat actcttccag cagcctcagc tctttcagcc ggctcagcac ctctttggcg atatagcgcc ccacctgctg cacctccagc gagtgggtca ggcgagtgcg caccgccgcg ttgcgctcga gcgggaagac ctgagttttt tgctgcaggc ggcggattgc aggcgagttg acgatccgtc cccgatcgct ttcaaagatc cgcaggatgt cgcgctccgt ctccacccgc ggcggcgaac ggaagcgtcg acgccagttt atcttgttgc gaaaatcaat ctttgccata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMA KIDFRNKINW RRRFRSPPRV ETERDILRIF ESDRGRIVNS PAIRRLQQKT QVFPLERNAA VRTRLTHSLE VQQVGRYIAK EVLSRLKEQR LLEEYGLEEL TGPFESVVEM ACLMHDIGNP PFGHFGEAAI NDWFRQRLAP GDALGQPLID DRCEVQALRL HDGETSLNAL RRKVRQDLCS FEGNAQGIRL VHTLMRMNLT WAQVGCILKY TRPAWWSEET PASHSYLMKK PGYYLAEEEY VARLRKELDL APYNRFPLTW IMEAADDISY CVADLEDAVE KRIFSADQLY QHLYDAWGSH EKGSLFSQVV ENAWEKSRAN YLKQSAEDQF FMYLRVNTLN KLVPYAARRF IDNLPAIFTG DFNHALLEDD SDCSQLLELY KNVAMKQVFS HPDVEQLELQ GYRVISGLLD IYQPLLKLSL EDFSELVAQE RVRRLPIASR LYQKLSTRHR LAYVEAVNKL ARTAPEFALM EYYYRCRLIQ DYISGMTDLY AWDEYRRLMA VE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa agattgattt tcgcaacaag ataaactggc gtcgacgctt ccgttcgccg ccgcgggtgg agacggagcg cgacatcctg cggatctttg aaagcgatcg gggacggatc gtcaactcgc cggcaatccg ccgcctgcag caaaaaactc aggtcttccc gctcgagcgc aacgcggcgg tgcgcactcg cctgacccac tcgctggagg tgcagcaggt ggggcgctat atcgccaaag aggtgctgag ccggctgaaa gagcagaggc tgctggaaga gtatggcctg gaagagctga ccgggccatt cgagagcgtg gtggagatgg cctgcctgat gcacgatatc ggcaatcccc cgtttggcca ttttggcgaa gcggcgatta acgactggtt ccgtcagcgc ctggcgcccg gcgatgcgct cgggcagccg ctgatcgacg atcgctgcga agtgcaggcc ttacgcctgc atgacggaga gacatcgctt aatgcgctgc gccgtaaagt gcgccaggat ctctgcagct ttgagggcaa cgcgcagggc attcgtctgg tccatacgct aatgcgtatg aacctgacct gggcgcaggt gggatgtatc ctgaaatata ctcgtccggc gtggtggtct gaggagacgc cggccagcca cagttattta atgaagaaac ctggctatta tttagccgaa gaagagtatg tcgcgcgttt gcgtaaagaa ctggatctcg cgccttacaa tcggtttcca ttaacctgga ttatggaggc tgcggatgac atctcatatt gcgtcgcgga tctcgaggac gcggtggaaa aacgtatttt tagcgcagac cagctctatc agcatcttta cgacgcctgg ggcagtcatg aaaaaggctc gttattttcg caggtagtag aaaatgcctg ggagaaatcg cgagccaatt atctgaagca gagcgcggaa gatcagtttt ttatgtattt gcgggtgaat acgctgaata agctggtgcc ctacgcggcg cgccgattta ttgacaacct gccggcaatc ttcacggggg actttaatca tgcgttgctg gaggacgaca gcgattgcag tcagctactg gagctctata aaaatgtggc gatgaaacag gtctttagcc atccggatgt tgagcagctg gaattgcagg gctaccgggt gatcagcggc ctgttggata tctatcagcc gctgctgaaa ttatcgctgg aggatttcag cgaattagta gcgcaggagc gggtacgacg tttacccatt gcctcccggc tgtatcagaa actttcgacc cgtcaccggc tggcctatgt tgaagcggta aataaactgg cgcgtacggc gcccgaattt gcgctgatgg aatactatta tcgctgccgt ctgattcagg actatatcag cgggatgacg gatttgtatg cgtgggatga atatcggcga ctcatggcgg tggaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for KlpnC.20460.a.B1.PW39076
PURIFICATION DATe: 8/3/2021
CONCENTRATION: 16mg/ml
OBSERVED MW: 60kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 30
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA KIDFRNKINW RRRFRSPPRV ETERDILRIF ESDRGRIVNS PAIRRLQQKT QVFPLERNAA VRTRLTHSLE VQQVGRYIAK EVLSRLKELR LLEEYGLEEL TGPFESVVEM ACLMHDIGNP PFGHFGEAAI NDWFRQRLAP GDALGQPLTD DRCEVQALRL HDGETSLNAL RRKVRQDLCS FXGNAXGIRL VHTLMRMNLT WAQVGCILKY TRPAWWSEET PASHSYLMKK PGYYLAEEEY VARLRKELDL APYNRFPLTW IMEAADDISY CVADLEDAVE KRIFSAEQLY QHLYDAWGSH EKGSLFSQVV ENAWEKSRAN YLKQSAEDQF FMYLRVNTLN KLVPYAARRF IDNLPAIFTG DFNHALLEDD SDCSQLLELY KNVAMKQVFS HPDVEQLELQ GYRVISGLLD IYQPLLKLSL EDFSELVAQE RVRRLPIASR LYQKLSTRHR LAYVEAVNKL ARTAPEFALM EYYYRCRLIQ DYISGMTDLY AWDE
Validated NT Sequence
attcatccca cgcatacaaa tccgtcatcc cgctgatata gtcctgaatc agacggcagc gataatagta ttccatcagc gcaaattcgg gcgccgtacg cgccagttta tttaccgctt caacataggc cagccggtga cgggtcgaaa gtttctgata cagccgggag gcaatgggta aacgtcgtac ccgctcctgc gctactaatt cgctgaaatc ctccagcgat aatttcagca gcggctgata gatatccaac aggccgctga tcacccggta gccctgcaat tccagctgct caacatccgg atggctaaag acctgtttca tcgccacatt tttatagagc tccagtagct gactgcaatc gctgtcgtcc tccagcaacg catgattaaa gtcccccgtg aagattgccg gcaggttgtc aataaatcgg cgcgccgcgt agggcaccag cttattcagc gtattcaccc gcaaatacat aaaaaactga tcttccgcgc tctgcttcag ataattggct cgcgatttct cccaggcatt ttctactacc tgcgaaaata acgagccttt ttcatgactg ccccaggcgt cgtaaagatg ctgatagagc tgttctgcgc taaaaatacg tttttccacc gcgtcctcga gatccgcgac gcaatatgag atgtcatccg cagcctccat aatccaggtt aatggaaacc gattgtaagg cgcgagatcc agttctttac gcaaacgcgc gacatactct tcttcggcta aataatagcc aggtttcttc attaaataac tgtggctggc cggcgtctcc tcagaccacc acgccggacg agtatatttc aggatacatc ccacctgcgc ccaggtcagg ttcatacgca ttagcgtatg gaccagacga atgccntgcg cgttgccntc aaagctgcag agatcctggc gcactttacg gcgcagcgca ttaagcgatg tctctccgtc atgcaggcgt aaggcctgca cttcgcagcg atcgtcggtc agcggctgcc cgagcgcatc gccgggcgcc aggcgctgac ggaaccagtc gttaatcgcc gcttcgccaa aatggccaaa cgggggattg ccgatatcgt gcatcaggca ggccatctcc accacgctct cgaatggccc ggtcagctct tccaggccat actcttccag cagcctcagc tctttcagcc ggctcagcac ctctttggcg atatagcgcc ccacctgctg cacctccagc gagtgggtca ggcgagtgcg caccgccgcg ttgcgctcga gcgggaagac ctgagttttt tgctgcaggc ggcggattgc aggcgagttg acgatccgtc cccgatcgct ttcaaagatc cgcaggatgt cgcgctccgt ctccacccgc ggcggcgaac ggaagcgtcg acgccagttt atcttgttgc gaaaatcaat ctttgccata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMA KIDFRNKINW RRRFRSPPRV ETERDILRIF ESDRGRIVNS PAIRRLQQKT QVFPLERNAA VRTRLTHSLE VQQVGRYIAK EVLSRLKEQR LLEEYGLEEL TGPFESVVEM ACLMHDIGNP PFGHFGEAAI NDWFRQRLAP GDALGQPLID DRCEVQALRL HDGETSLNAL RRKVRQDLCS FEGNAQGIRL VHTLMRMNLT WAQVGCILKY TRPAWWSEET PASHSYLMKK PGYYLAEEEY VARLRKELDL APYNRFPLTW IMEAADDISY CVADLEDAVE KRIFSADQLY QHLYDAWGSH EKGSLFSQVV ENAWEKSRAN YLKQSAEDQF FMYLRVNTLN KLVPYAARRF IDNLPAIFTG DFNHALLEDD SDCSQLLELY KNVAMKQVFS HPDVEQLELQ GYRVISGLLD IYQPLLKLSL EDFSELVAQE RVRRLPIASR LYQKLSTRHR LAYVEAVNKL ARTAPEFALM EYYYRCRLIQ DYISGMTDLY AWDEYRRLMA VE
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA AGATTGATTT TCGCAACAAG ATAAACTGGC GTCGACGCTT CCGTTCGCCG CCGCGGGTGG AGACGGAGCG CGACATCCTG CGGATCTTTG AAAGCGATCG GGGACGGATC GTCAACTCGC CGGCAATCCG CCGCCTGCAG CAAAAAACTC AGGTCTTCCC GCTCGAGCGC AACGCGGCGG TGCGCACTCG CCTGACCCAC TCGCTGGAGG TGCAGCAGGT GGGGCGCTAT ATCGCCAAAG AGGTGCTGAG CCGGCTGAAA GAGCAGAGGC TGCTGGAAGA GTATGGCCTG GAAGAGCTGA CCGGGCCATT CGAGAGCGTG GTGGAGATGG CCTGCCTGAT GCACGATATC GGCAATCCCC CGTTTGGCCA TTTTGGCGAA GCGGCGATTA ACGACTGGTT CCGTCAGCGC CTGGCGCCCG GCGATGCGCT CGGGCAGCCG CTGATCGACG ATCGCTGCGA AGTGCAGGCC TTACGCCTGC ATGACGGAGA GACATCGCTT AATGCGCTGC GCCGTAAAGT GCGCCAGGAT CTCTGCAGCT TTGAGGGCAA CGCGCAGGGC ATTCGTCTGG TCCATACGCT AATGCGTATG AACCTGACCT GGGCGCAGGT GGGATGTATC CTGAAATATA CTCGTCCGGC GTGGTGGTCT GAGGAGACGC CGGCCAGCCA CAGTTATTTA ATGAAGAAAC CTGGCTATTA TTTAGCCGAA GAAGAGTATG TCGCGCGTTT GCGTAAAGAA CTGGATCTCG CGCCTTACAA TCGGTTTCCA TTAACCTGGA TTATGGAGGC TGCGGATGAC ATCTCATATT GCGTCGCGGA TCTCGAGGAC GCGGTGGAAA AACGTATTTT TAGCGCAGAC CAGCTCTATC AGCATCTTTA CGACGCCTGG GGCAGTCATG AAAAAGGCTC GTTATTTTCG CAGGTAGTAG AAAATGCCTG GGAGAAATCG CGAGCCAATT ATCTGAAGCA GAGCGCGGAA GATCAGTTTT TTATGTATTT GCGGGTGAAT ACGCTGAATA AGCTGGTGCC CTACGCGGCG CGCCGATTTA TTGACAACCT GCCGGCAATC TTCACGGGGG ACTTTAATCA TGCGTTGCTG GAGGACGACA GCGATTGCAG TCAGCTACTG GAGCTCTATA AAAATGTGGC GATGAAACAG GTCTTTAGCC ATCCGGATGT TGAGCAGCTG GAATTGCAGG GCTACCGGGT GATCAGCGGC CTGTTGGATA TCTATCAGCC GCTGCTGAAA TTATCGCTGG AGGATTTCAG CGAATTAGTA GCGCAGGAGC GGGTACGACG TTTACCCATT GCCTCCCGGC TGTATCAGAA ACTTTCGACC CGTCACCGGC TGGCCTATGT TGAAGCGGTA AATAAACTGG CGCGTACGGC GCCCGAATTT GCGCTGATGG AATACTATTA TCGCTGCCGT CTGATTCAGG ACTATATCAG CGGGATGACG GATTTGTATG CGTGGGATGA ATATCGGCGA CTCATGGCGG TGGAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT