SSGCID: Target Page

KlpnC.20459.a

6-phospho-beta-glucosidase

CENTER ID:	KlpnC.20459.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20459.a.B1.GE44426	Full length(KlpnC.20459.a)		1	448

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.2079
UniProt:	A0A0H3GRL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQKLKVVTI GGGSSYTPEL LEGFLKRYHE LPVSELWLVD VEEGQEKLDI IHALCQRMVE KAGVPMKVYK TLDRRAALQG ADFVTTQLRV GQLKAREKDE RIPLSHGYLG QETNGAGGLF KGLRTIPVIF DIVKDVQEIC PDAWIINFTN PAGMVTEAVY RHTNFKRFIG VCNIPIGMKM FITDVLQLSP SDELNIDLFG LNHLVFVRDV LVNGVSRFDE LLDGVASGRL TANSVKNIFD LPFSEGLIRS LRLIPCSYLL YYFKPKEMLA IEMGEYYKGG ARAQVVQKVE KQLFELYKNP DLNVKPKELE QRGGAYYSDA ACEVINAIYN DKQTEHYVNI PHHGHVDNIP ADWAVEMSCT LGRDGAKPTP RITHFDEKVL GLIYTIKGFE VAASQAAISG ELNDVLLALN LSPLIHSDRD AEQLAREMIL AHEKWLPNFA ATIEKLKS

NT Sequence

ATGAGCCAGA AATTAAAAGT AGTCACTATT GGCGGCGGCA GCAGCTATAC CCCGGAATTA CTGGAAGGAT TTCTGAAACG TTATCATGAA CTGCCGGTAA GCGAACTGTG GTTGGTAGAC GTCGAAGAGG GACAGGAGAA GCTCGATATT ATCCACGCGC TGTGCCAGCG GATGGTGGAA AAAGCCGGCG TGCCGATGAA AGTCTATAAA ACGCTGGATC GCCGCGCAGC GCTGCAGGGC GCAGATTTCG TCACCACCCA GCTGCGCGTG GGCCAGCTGA AGGCGCGTGA AAAAGATGAG CGCATCCCGC TGAGTCACGG CTACCTGGGC CAGGAGACCA ACGGGGCGGG CGGGTTGTTT AAAGGACTGC GCACCATCCC GGTGATTTTC GACATCGTGA AAGATGTGCA GGAAATTTGC CCCGACGCAT GGATCATCAA CTTTACCAAC CCGGCGGGCA TGGTCACCGA GGCGGTCTAT CGCCACACCA ATTTCAAACG CTTTATCGGG GTGTGCAACA TTCCTATCGG CATGAAGATG TTCATCACCG ACGTGCTGCA GCTGAGCCCG AGCGATGAGC TGAATATCGA CCTGTTTGGT CTGAACCACC TGGTGTTCGT CCGCGACGTG CTGGTTAACG GCGTCTCGCG CTTCGATGAA CTGCTGGACG GCGTGGCCTC CGGCCGTCTG ACCGCGAACT CGGTGAAAAA CATCTTCGAC CTGCCGTTTA GCGAAGGGCT GATCCGCTCC CTGCGCCTGA TCCCATGCTC TTACCTGCTC TACTACTTTA AGCCAAAAGA GATGCTGGCG ATTGAGATGG GCGAGTACTA CAAAGGCGGT GCGCGCGCCC AGGTGGTGCA GAAGGTGGAG AAACAGCTGT TCGAGCTGTA TAAAAACCCG GATCTTAACG TTAAGCCGAA GGAGCTGGAG CAGCGCGGCG GGGCCTATTA CTCTGATGCC GCTTGCGAAG TAATCAATGC CATCTATAAC GACAAGCAGA CTGAGCACTA TGTGAATATC CCGCATCATG GTCATGTCGA TAATATCCCG GCGGACTGGG CGGTAGAGAT GAGCTGCACC TTAGGCCGCG ACGGCGCGAA GCCGACGCCG CGCATTACTC ATTTCGATGA GAAGGTGTTA GGGCTTATCT ACACCATCAA GGGGTTCGAA GTGGCGGCCA GCCAGGCGGC GATCAGCGGT GAGCTGAACG ATGTCCTGTT GGCGCTGAAC TTAAGCCCGC TGATCCATTC CGATCGTGAC GCCGAACAGC TGGCGCGGGA GATGATTCTG GCGCATGAAA AATGGCTGCC GAACTTTGCG GCGACGATCG AAAAACTAAA ATCG

Details for KlpnC.20459.a.B1.GE44426

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMS QKLKVVTIGG GSSYTPELLE GFLKRYHELP VSELWLVDVE EGQEKLDIIH ALCQRMVEKA GVPMKVYKTL DRRAALQGAD FVTTQLRVGQ LKAREKDERI PLSHGYLGQE TNGAGGLFKG LRTIPVIFDI VKDVQEICPD AWIINFTNPA GMVTEAVYRH TNFKRFIGVC NIPIGMKMFI TDVLQLSPSD ELNIDLFGLN HLVFVRDVLV NGVSRFDELL DGVASGRLTA NSVKNIFDLP FSEGLIRSLR LIPCSYLLYY FKPKEMLAIE MGEYYKGGAR AQVVQKVEKQ LFELYKNPDL NVKPKELEQR GGAYYSDAAC EVINAIYNDK QTEHYVNIPH HGHVDNIPAD WAVEMSCTLG RDGAKPTPRI THFDEKVLGL IYTIKGFEVA ASQAAISGEL NDVLLALNLS PLIHSDRDAE QLAREMILAH EKWLPNFAXX

Validated NT Sequence

tnnnnnnngc aaagttcggc agccattttt catgcgccag aatcatctcc cgcgccagct gttcggcgtc acgatcggaa tggatcagcg ggcttaagtt cagcgccaac aggacatcgt tcagctcacc gctgatcgcc gcctggctgg ccgccacttc gaaccccttg atggtgtaga taagccctaa caccttctca tcgaaatgag taatgcgcgg cgtcggcttc gcgccgtcgc ggcctaaggt gcagctcatc tctaccgccc agtccgccgg gatattatcg acatggccat gatgcgggat attcacatag tgctcagtct gcttgtcgtt atagatggca ttgattactt cgcaagcggc atcagagtaa taggccccgc cgcgctgctc cagctccttc ggcttaacgt taagatccgg gtttttatac agctcgaaca gctgtttctc caccttctgc accacctggg cgcgcgcacc gcctttgtag tactcgccca tctcaatcgc cagcatctct tttggcttaa agtagtagag caggtaagag catgggatca ggcgcaggga gcggatcagc ccttcgctaa acggcaggtc gaagatgttt ttcaccgagt tcgcggtcag acggccggag gccacgccgt ccagcagttc atcgaagcgc gagacgccgt taaccagcac gtcgcggacg aacaccaggt ggttcagacc aaacaggtcg atattcagct catcgctcgg gctcagctgc agcacgtcgg tgatgaacat cttcatgccg ataggaatgt tgcacacgcc gataaagcgt ttgaaattgg tgtggcgata gaccgcctcg gtgaccatgc ccgccgggtt ggtgaagttg atgatccatg cgtcggggca aatttcctgc acatctttca cgatatcgaa aatcaccggg atggtgcgca gtcctttaaa caacccgccc gccccgttgg tctcctggcc caggtagccg tggctcagcg ggatgcgctc atctttttca cgcgccttca gctggcccac gcgcagctgg gtggtgacga aatctgcgcc ctgcagcgct gcgcggcgat ccagcgtttt atagactttc atcggcacgc cggctttttc taccatccgc tggcacagcg cgtggataat atcgagcttc tcctggccct cttcgacgtc taccaaccac agttcgctta ccggcagttc atgataacgt ttcagaaatc cttccagtaa ttccggggta tagctgctgc cgccgccaat agtgactact tttaatttct ggctcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc agaaattaaa agtagtcact attggcggcg gcagcagcta taccccggaa ttactggaag gatttctgaa acgttatcat gaactgccgg taagcgaact gtggttggta gacgtcgaag agggacagga gaagctcgat attatccacg cgctgtgcca gcggatggtg gaaaaagccg gcgtgccgat gaaagtctat aaaacgctgg atcgccgcgc agcgctgcag ggcgcagatt tcgtcaccac ccagctgcgc gtgggccagc tgaaggcgcg tgaaaaagat gagcgcatcc cgctgagtca cggctacctg ggccaggaga ccaacggggc gggcgggttg tttaaaggac tgcgcaccat cccggtgatt ttcgacatcg tgaaagatgt gcaggaaatt tgccccgacg catggatcat caactttacc aacccggcgg gcatggtcac cgaggcggtc tatcgccaca ccaatttcaa acgctttatc ggggtgtgca acattcctat cggcatgaag atgttcatca ccgacgtgct gcagctgagc ccgagcgatg agctgaatat cgacctgttt ggtctgaacc acctggtgtt cgtccgcgac gtgctggtta acggcgtctc gcgcttcgat gaactgctgg acggcgtggc ctccggccgt ctgaccgcga actcggtgaa aaacatcttc gacctgccgt ttagcgaagg gctgatccgc tccctgcgcc tgatcccatg ctcttacctg ctctactact ttaagccaaa agagatgctg gcgattgaga tgggcgagta ctacaaaggc ggtgcgcgcg cccaggtggt gcagaaggtg gagaaacagc tgttcgagct gtataaaaac ccggatctta acgttaagcc gaaggagctg gagcagcgcg gcggggccta ttactctgat gccgcttgcg aagtaatcaa tgccatctat aacgacaagc agactgagca ctatgtgaat atcccgcatc atggtcatgt cgataatatc ccggcggact gggcggtaga gatgagctgc accttaggcc gcgacggcgc gaagccgacg ccgcgcatta ctcatttcga tgagaaggtg ttagggctta tctacaccat caaggggttc gaagtggcgg ccagccaggc ggcgatcagc ggtgagctga acgatgtcct gttggcgctg aacttaagcc cgctgatcca ttccgatcgt gacgccgaac agctggcgcg ggagatgatt ctggcgcatg aaaaatggct gccgaacttt gcggcgacga tcgaaaaact aaaatcgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at