KlpnC.20457.a

S-adenosylmethionine decarboxylase proenzyme

CENTER ID: KlpnC.20457.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20457.a.B1.GE44424 Full length(KlpnC.20457.a) 1 264
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.821
UniProt: A0A0H3GIB3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKLKLHGFN NLTKSLSFCI YDICYAKTAE ERDGYIAYID ELYNANRLTE ILTETCSIIG ANILNIARQD YEPQGASVTI LVSEEPVDPK LIDQTEHPGP LPETVVAHLD KSHICVHTYP ESHPEGGLCT FRADIEVSTC GVISPLKALN YLIHQLESDI VTIDYRVRGF TRDINGMKHF IDHEINSIQN FMSDDMKSLY DMVDVNVYQE NIFHTKMLLK EFDLKHYMFH TRPEELTAEE RKVITDLLWK EMREIYYGRN IPAV
NT Sequence
TTGAAAAAAT TGAAGCTGCA CGGCTTTAAC AACCTGACCA AAAGCCTGAG TTTTTGTATC TACGATATCT GTTACGCCAA AACGGCAGAA GAGCGCGACG GCTACATCGC CTATATCGAC GAACTCTATA ATGCCAATCG CCTGACGGAG ATCCTGACAG AAACCTGTTC TATTATTGGC GCCAATATCC TGAATATCGC CCGTCAGGAT TATGAACCCC AGGGCGCCAG CGTGACCATT CTGGTCAGCG AAGAGCCTGT CGATCCCAAG CTGATTGACC AGACCGAACA TCCTGGCCCG CTACCGGAAA CCGTGGTCGC CCATCTCGAT AAGAGCCATA TCTGCGTGCA TACCTACCCG GAGAGCCATC CGGAGGGCGG CCTGTGCACC TTCCGCGCCG ATATCGAAGT TTCAACGTGC GGCGTCATTT CCCCGCTTAA AGCGCTGAAC TATCTGATTC ACCAGCTGGA ATCGGACATT GTGACCATTG ACTACCGTGT GCGCGGCTTT ACCCGCGATA TCAATGGGAT GAAGCATTTT ATCGATCATG AAATCAATTC GATTCAGAAC TTTATGTCCG ACGATATGAA GTCGTTGTAT GACATGGTCG ACGTCAACGT TTATCAGGAA AATATCTTCC ATACCAAAAT GTTGCTCAAG GAGTTCGACC TTAAGCACTA CATGTTCCAT ACCCGACCGG AAGAGTTGAC TGCCGAAGAG CGCAAGGTCA TTACCGACCT GCTATGGAAA GAGATGCGCG AGATTTACTA CGGCCGCAAT ATTCCTGCCG TG
Details for KlpnC.20457.a.B1.GE44424
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KKLKLHGFNN LTKSLSFCIY DICYAKTAEE RDGYIAYIDE LYNANRLTEI LTETCSIIGA NILNIARQDY EPQGASVTIL VSEEPVDPKL IDQTEHPGPL PETVVAHLDK SHICVHTYPE SHPEGGLCTF RADIEVSTCG VISPLKALNY LIHQLESDIV TIDYRVRGFT RDINGMKHFI DHEINSIQNF MSDDMKSLYD MVDVNVYQEN IFHTKMLLKE FDLKHYMFHT RPEELTAEER KVITDLLWKE MREIYYGRNI PAV
Validated NT Sequence
atcttactca cttacacggc aggaatattg cggccgtagt aaatctcgcg catctctttc cataacaggt cggtaatgac cttgcgctct tcggcagtca actcttccgg tcgggtatgg aacatgtagt gcttaaggtc gaactccttg agcaacattt tggtatggaa gatattttcc tgataaacgt tgacgtcgac catgtcatac aacgacttca tatcgtcgga cataaagttc tgaatcgaat tgatttcatg atcgataaaa tgcttcatcc cattgatatc gcgggtaaag ccgcgcacac ggtagtcaat ggtcacaatg tccgattcca gctggtgaat cagatagttc agcgctttaa gcggggaaat gacgccgcac gttgaaactt cgatatcggc gcggaaggtg cacaggccgc cctccggatg gctctccggg taggtatgca cgcagatatg gctcttatcg agatgggcga ccacggtttc cggtagcggg ccaggatgtt cggtctggtc aatcagcttg ggatcgacag gctcttcgct gaccagaatg gtcacgctgg cgccctgggg ttcataatcc tgacgggcga tattcaggat attggcgcca ataatagaac aggtttctgt caggatctcc gtcaggcgat tggcattata gagttcgtcg atataggcga tgtagccgtc gcgctcttct gccgttttgg cgtaacagat atcgtagata caaaaactca ggcttttggt caggttgtta aagccgtgca gcttcaattt tttcaacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMK KLKLHGFNNL TKSLSFCIYD ICYAKTAEER DGYIAYIDEL YNANRLTEIL TETCSIIGAN ILNIARQDYE PQGASVTILV SEEPVDPKLI DQTEHPGPLP ETVVAHLDKS HICVHTYPES HPEGGLCTFR ADIEVSTCGV ISPLKALNYL IHQLESDIVT IDYRVRGFTR DINGMKHFID HEINSIQNFM SDDMKSLYDM VDVNVYQENI FHTKMLLKEF DLKHYMFHTR PEELTAEERK VITDLLWKEM REIYYGRNIP AV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgaaaa aattgaagct gcacggcttt aacaacctga ccaaaagcct gagtttttgt atctacgata tctgttacgc caaaacggca gaagagcgcg acggctacat cgcctatatc gacgaactct ataatgccaa tcgcctgacg gagatcctga cagaaacctg ttctattatt ggcgccaata tcctgaatat cgcccgtcag gattatgaac cccagggcgc cagcgtgacc attctggtca gcgaagagcc tgtcgatccc aagctgattg accagaccga acatcctggc ccgctaccgg aaaccgtggt cgcccatctc gataagagcc atatctgcgt gcatacctac ccggagagcc atccggaggg cggcctgtgc accttccgcg ccgatatcga agtttcaacg tgcggcgtca tttccccgct taaagcgctg aactatctga ttcaccagct ggaatcggac attgtgacca ttgactaccg tgtgcgcggc tttacccgcg atatcaatgg gatgaagcat tttatcgatc atgaaatcaa ttcgattcag aactttatgt ccgacgatat gaagtcgttg tatgacatgg tcgacgtcaa cgtttatcag gaaaatatct tccataccaa aatgttgctc aaggagttcg accttaagca ctacatgttc catacccgac cggaagagtt gactgccgaa gagcgcaagg tcattaccga cctgctatgg aaagagatgc gcgagattta ctacggccgc aatattcctg ccgtgtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat