KlpnC.20454.a

Putative protease

CENTER ID: KlpnC.20454.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20454.a.B1.GE44421 Full length(KlpnC.20454.a) 1 453
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20454.a.B1.PW39064 Full length(KlpnC.20454.a) 1 453

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.3499
UniProt: A0A0H3GQR9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFKPELLSPA GTLKNMRYAF AYGADAVYAG QPRYSLRVRN NEFNHENLQL GINEAHALGK KFYVVVNIAP HNAKLKTFIR DLKPVVEMGP DALIMSDPGL IMLVREHFPE MPIHLSVQAN AVNWATVKFW QQMGLTRVIL SRELSLDEIE EIRRQVPEME IEIFVHGALC MAYSGRCLLS GYINKRDPNQ GTCTNACRWE YNVAEGKEDD VGNIVHKYEP IPVQTVEPTL GIGAPTDKVF MIEEAKRPGE YMTAFEDEHG TYIMNSKDLR AIAHVERLTQ MGVHSLKIEG RTKSFYYCAR TAQVYRKAID DAAAGKPFDT SLLETLEGLA HRGYTEGFLR RHTHDDYQNY EYGYSVSERQ QFVGEFTGER NGPLAAVAVK NKFSVGDSLE LMTPQGNVNF TLEHMENGKG ETMPVAPGDG YTVWLPVPDD LPLQYALLMR NFTGQTTRNP HGN
NT Sequence
ATGTTTAAAC CAGAACTCCT CTCCCCGGCG GGAACGCTGA AGAATATGCG TTACGCTTTC GCCTATGGCG CCGATGCCGT TTATGCCGGT CAGCCGCGCT ACTCGCTGCG CGTGCGTAAC AACGAATTCA ATCACGAAAA TCTGCAGCTT GGCATCAATG AAGCCCACGC GCTGGGTAAA AAATTCTATG TGGTGGTCAA CATCGCCCCG CATAACGCCA AGCTGAAAAC GTTTATTCGC GATCTGAAGC CGGTGGTGGA GATGGGTCCG GACGCGCTGA TCATGTCCGA TCCGGGTTTA ATCATGCTGG TGCGGGAACA CTTCCCGGAG ATGCCGATCC ATCTTTCAGT GCAGGCCAAC GCCGTCAACT GGGCGACGGT CAAATTCTGG CAGCAGATGG GGCTGACCCG CGTGATCCTG TCGCGTGAAC TGTCGCTGGA TGAAATCGAA GAGATTCGCC GCCAGGTGCC GGAGATGGAG ATTGAGATTT TCGTCCACGG CGCGCTATGC ATGGCCTACT CCGGCCGCTG CCTGCTCTCC GGGTATATCA ACAAGCGCGA CCCGAACCAG GGGACCTGCA CCAACGCCTG CCGCTGGGAA TACAACGTGG CGGAAGGCAA AGAGGACGAC GTCGGCAACA TCGTCCACAA ATATGAGCCG ATCCCGGTGC AGACCGTGGA GCCTACCCTC GGCATCGGCG CGCCGACCGA TAAAGTCTTT ATGATTGAGG AAGCCAAACG TCCGGGCGAA TACATGACCG CCTTTGAGGA CGAACACGGC ACCTACATCA TGAACTCGAA AGATCTGCGG GCTATCGCCC ACGTTGAGCG TCTGACCCAG ATGGGCGTTC ACTCGCTGAA GATCGAAGGC CGCACCAAAT CCTTCTACTA CTGCGCGCGC ACCGCTCAGG TTTACCGCAA GGCGATCGAC GATGCCGCCG CCGGCAAACC GTTCGATACC AGCCTGCTGG AGACCCTGGA GGGGCTGGCC CACCGCGGGT ACACCGAAGG CTTCCTGCGC CGCCATACCC ATGACGATTA TCAGAACTAC GAGTACGGCT ACTCCGTCTC CGAGCGCCAG CAGTTTGTCG GCGAATTCAC CGGAGAGCGT AACGGCCCGC TGGCGGCCGT GGCGGTCAAA AATAAGTTCA GCGTCGGCGA CAGCCTTGAG CTGATGACCC CGCAGGGTAA CGTCAACTTC ACCCTTGAGC ATATGGAGAA TGGCAAAGGC GAAACCATGC CGGTGGCCCC GGGCGATGGC TATACCGTCT GGTTGCCTGT GCCGGACGAC CTGCCCCTGC AGTATGCCCT GCTGATGCGC AACTTTACTG GTCAAACAAC CAGAAATCCG CACGGTAAC
Details for KlpnC.20454.a.B1.GE44421
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMF KPELLSPAGT LKNMRYAFAY GADAVYAGQP RYSLRVRNNE FNHENLQLGI NEAHALGKKF YVVVNIAPHN AKLKTFIRDL KPVVEMGPDA LIMSDPGLIM LVREHFPEMP IHLSVQANAV NWATVKFWQQ MGLTRVILSR ELSLDEIEEI RRQVPEMEIE IFVHGALCMA YSGRCLLSGY INKRDPNQGT CTNACRWEYN VAEGKEDDVG NIVHKYEPIP VQTVEPTLGI GAPTDKVFMI EEAKRPGEYM TAFEDEHGTY IMNSKDLRAI AHVERLTQMG VHSLKIEGRT KSFYYCARTA QVYRKAIDDA AAGKPFDTSL LETLEGLAHR GYTEGFLRRH THDDYQNYEY GYSVSERQQF VGEFTGERNG PLAAVAVKNK FSVGDSLELM TPQGNVNFTL EHMENGKGET MPVAPGDGYT VWLPVPDDLP LQYALLMRNF TGQ
Validated NT Sequence
tgnttgacca gtaaagttgc gcatcagcag ggcatactgc aggggcaggt cgtccggcac aggcaaccag acggtatagc catcgcccgg ggccaccggc atggtttcgc ctttgccatt ctccatatgc tcaagggtga agttgacgtt accctgcggg gtcatcagct caaggctgtc gccgacgctg aacttatttt tgaccgccac ggccgccagc gggccgttac gctctccggt gaattcgccg acaaactgct ggcgctcgga gacggagtag ccgtactcgt agttctgata atcgtcatgg gtatggcggc gcaggaagcc ttcggtgtac ccgcggtggg ccagcccctc cagggtctcc agcaggctgg tatcgaacgg tttgccggcg gcggcatcgt cgatcgcctt gcggtaaacc tgagcggtgc gcgcgcagta gtagaaggat ttggtgcggc cttcgatctt cagcgagtga acgcccatct gggtcagacg ctcaacgtgg gcgatagccc gcagatcttt cgagttcatg atgtaggtgc cgtgttcgtc ctcaaaggcg gtcatgtatt cgcccggacg tttggcttcc tcaatcataa agactttatc ggtcggcgcg ccgatgccga gggtaggctc cacggtctgc accgggatcg gctcatattt gtggacgatg ttgccgacgt cgtcctcttt gccttccgcc acgttgtatt cccagcggca ggcgttggtg caggtcccct ggttcgggtc gcgcttgttg atatacccgg agagcaggca gcggccggag taggccatgc acagcgcgcc gtggacgaaa atctcaatct ccatctccgg cacctggcgg cgaatctctt cgatttcatc cagcgacagt tcacgcgaca ggatcacgcg ggtgagcccc atctgctgcc agaatttgac cgtcgcccag ttgacggcgt tggcctgcac tgaaagatgg atcggcatct ccgggaagtg ttcccgcacc agcatgatta aacccggatc ggacatgatc agcgcgtccg gacccatctc caccaccggc ttcagatcgc gaataaacgt tttcagcttg gcgttatgcg gggcgatgtt gaccaccaca tagaattttt tacccagcgc gtgggcttca ttgatgccga gctgcagatt ttcgtgattg aattcgttgt tacgcacgcg cagcgagtag cgcggctggc cggcataaac ggcatcggcg ccataggcga aagcgtaacg catattcttc agcgttcccg ccggggagag gagttctggt ttaaacatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMF KPELLSPAGT LKNMRYAFAY GADAVYAGQP RYSLRVRNNE FNHENLQLGI NEAHALGKKF YVVVNIAPHN AKLKTFIRDL KPVVEMGPDA LIMSDPGLIM LVREHFPEMP IHLSVQANAV NWATVKFWQQ MGLTRVILSR ELSLDEIEEI RRQVPEMEIE IFVHGALCMA YSGRCLLSGY INKRDPNQGT CTNACRWEYN VAEGKEDDVG NIVHKYEPIP VQTVEPTLGI GAPTDKVFMI EEAKRPGEYM TAFEDEHGTY IMNSKDLRAI AHVERLTQMG VHSLKIEGRT KSFYYCARTA QVYRKAIDDA AAGKPFDTSL LETLEGLAHR GYTEGFLRRH THDDYQNYEY GYSVSERQQF VGEFTGERNG PLAAVAVKNK FSVGDSLELM TPQGNVNFTL EHMENGKGET MPVAPGDGYT VWLPVPDDLP LQYALLMRNF TGQTTRNPHG N
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttta aaccagaact cctctccccg gcgggaacgc tgaagaatat gcgttacgct ttcgcctatg gcgccgatgc cgtttatgcc ggtcagccgc gctactcgct gcgcgtgcgt aacaacgaat tcaatcacga aaatctgcag cttggcatca atgaagccca cgcgctgggt aaaaaattct atgtggtggt caacatcgcc ccgcataacg ccaagctgaa aacgtttatt cgcgatctga agccggtggt ggagatgggt ccggacgcgc tgatcatgtc cgatccgggt ttaatcatgc tggtgcggga acacttcccg gagatgccga tccatctttc agtgcaggcc aacgccgtca actgggcgac ggtcaaattc tggcagcaga tggggctgac ccgcgtgatc ctgtcgcgtg aactgtcgct ggatgaaatc gaagagattc gccgccaggt gccggagatg gagattgaga ttttcgtcca cggcgcgcta tgcatggcct actccggccg ctgcctgctc tccgggtata tcaacaagcg cgacccgaac caggggacct gcaccaacgc ctgccgctgg gaatacaacg tggcggaagg caaagaggac gacgtcggca acatcgtcca caaatatgag ccgatcccgg tgcagaccgt ggagcctacc ctcggcatcg gcgcgccgac cgataaagtc tttatgattg aggaagccaa acgtccgggc gaatacatga ccgcctttga ggacgaacac ggcacctaca tcatgaactc gaaagatctg cgggctatcg cccacgttga gcgtctgacc cagatgggcg ttcactcgct gaagatcgaa ggccgcacca aatccttcta ctactgcgcg cgcaccgctc aggtttaccg caaggcgatc gacgatgccg ccgccggcaa accgttcgat accagcctgc tggagaccct ggaggggctg gcccaccgcg ggtacaccga aggcttcctg cgccgccata cccatgacga ttatcagaac tacgagtacg gctactccgt ctccgagcgc cagcagtttg tcggcgaatt caccggagag cgtaacggcc cgctggcggc cgtggcggtc aaaaataagt tcagcgtcgg cgacagcctt gagctgatga ccccgcaggg taacgtcaac ttcacccttg agcatatgga gaatggcaaa ggcgaaacca tgccggtggc cccgggcgat ggctataccg tctggttgcc tgtgccggac gacctgcccc tgcagtatgc cctgctgatg cgcaacttta ctggtcaaac aaccagaaat ccgcacggta actgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for KlpnC.20454.a.B1.PW39064
PURIFICATION DATe: 7/26/2021
CONCENTRATION: 34mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMF KPELLSPAGT LKNMRYAFAY GADAVYAGQP RYSLRVRNNE FNHENLQLGI NEAHALGKKF YVVVNIAPHN AKLKTFIRDL KPVVEMGPDA LIMSDPGLIM LVREHFPEMP IHLSVQANAV NWATVKFWQQ MGLTRVILSR ELSLDEIEEI RRQVPEMEIE IFVHGALCMA YSGRCLLSGY INKRDPNQGT CTNACRWEYN VAEGKEDDVG NIVHKYEPIP VQTVEPTLGI GAPTDKVFMI EEAKRPGEYM TAFEDEHGTY IMNSKDLRAI AHVERLTQMG VHSLKIEGRT KSFYYCARTA QVYRKAIDDA AAGKPFDTSL LETLEGLAHR GYTEGFLRRH THDDYQNYEY GYSVSERQQF VGEFTGERNG PLAAVAVKNK FSVGDSLELM TPQGNVNFTL EHMENGKGET MPVAPGDGYT VWLPVPDDLP LQYALLMRNF TGQ
Validated NT Sequence
tgnttgacca gtaaagttgc gcatcagcag ggcatactgc aggggcaggt cgtccggcac aggcaaccag acggtatagc catcgcccgg ggccaccggc atggtttcgc ctttgccatt ctccatatgc tcaagggtga agttgacgtt accctgcggg gtcatcagct caaggctgtc gccgacgctg aacttatttt tgaccgccac ggccgccagc gggccgttac gctctccggt gaattcgccg acaaactgct ggcgctcgga gacggagtag ccgtactcgt agttctgata atcgtcatgg gtatggcggc gcaggaagcc ttcggtgtac ccgcggtggg ccagcccctc cagggtctcc agcaggctgg tatcgaacgg tttgccggcg gcggcatcgt cgatcgcctt gcggtaaacc tgagcggtgc gcgcgcagta gtagaaggat ttggtgcggc cttcgatctt cagcgagtga acgcccatct gggtcagacg ctcaacgtgg gcgatagccc gcagatcttt cgagttcatg atgtaggtgc cgtgttcgtc ctcaaaggcg gtcatgtatt cgcccggacg tttggcttcc tcaatcataa agactttatc ggtcggcgcg ccgatgccga gggtaggctc cacggtctgc accgggatcg gctcatattt gtggacgatg ttgccgacgt cgtcctcttt gccttccgcc acgttgtatt cccagcggca ggcgttggtg caggtcccct ggttcgggtc gcgcttgttg atatacccgg agagcaggca gcggccggag taggccatgc acagcgcgcc gtggacgaaa atctcaatct ccatctccgg cacctggcgg cgaatctctt cgatttcatc cagcgacagt tcacgcgaca ggatcacgcg ggtgagcccc atctgctgcc agaatttgac cgtcgcccag ttgacggcgt tggcctgcac tgaaagatgg atcggcatct ccgggaagtg ttcccgcacc agcatgatta aacccggatc ggacatgatc agcgcgtccg gacccatctc caccaccggc ttcagatcgc gaataaacgt tttcagcttg gcgttatgcg gggcgatgtt gaccaccaca tagaattttt tacccagcgc gtgggcttca ttgatgccga gctgcagatt ttcgtgattg aattcgttgt tacgcacgcg cagcgagtag cgcggctggc cggcataaac ggcatcggcg ccataggcga aagcgtaacg catattcttc agcgttcccg ccggggagag gagttctggt ttaaacatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMF KPELLSPAGT LKNMRYAFAY GADAVYAGQP RYSLRVRNNE FNHENLQLGI NEAHALGKKF YVVVNIAPHN AKLKTFIRDL KPVVEMGPDA LIMSDPGLIM LVREHFPEMP IHLSVQANAV NWATVKFWQQ MGLTRVILSR ELSLDEIEEI RRQVPEMEIE IFVHGALCMA YSGRCLLSGY INKRDPNQGT CTNACRWEYN VAEGKEDDVG NIVHKYEPIP VQTVEPTLGI GAPTDKVFMI EEAKRPGEYM TAFEDEHGTY IMNSKDLRAI AHVERLTQMG VHSLKIEGRT KSFYYCARTA QVYRKAIDDA AAGKPFDTSL LETLEGLAHR GYTEGFLRRH THDDYQNYEY GYSVSERQQF VGEFTGERNG PLAAVAVKNK FSVGDSLELM TPQGNVNFTL EHMENGKGET MPVAPGDGYT VWLPVPDDLP LQYALLMRNF TGQTTRNPHG N
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTTA AACCAGAACT CCTCTCCCCG GCGGGAACGC TGAAGAATAT GCGTTACGCT TTCGCCTATG GCGCCGATGC CGTTTATGCC GGTCAGCCGC GCTACTCGCT GCGCGTGCGT AACAACGAAT TCAATCACGA AAATCTGCAG CTTGGCATCA ATGAAGCCCA CGCGCTGGGT AAAAAATTCT ATGTGGTGGT CAACATCGCC CCGCATAACG CCAAGCTGAA AACGTTTATT CGCGATCTGA AGCCGGTGGT GGAGATGGGT CCGGACGCGC TGATCATGTC CGATCCGGGT TTAATCATGC TGGTGCGGGA ACACTTCCCG GAGATGCCGA TCCATCTTTC AGTGCAGGCC AACGCCGTCA ACTGGGCGAC GGTCAAATTC TGGCAGCAGA TGGGGCTGAC CCGCGTGATC CTGTCGCGTG AACTGTCGCT GGATGAAATC GAAGAGATTC GCCGCCAGGT GCCGGAGATG GAGATTGAGA TTTTCGTCCA CGGCGCGCTA TGCATGGCCT ACTCCGGCCG CTGCCTGCTC TCCGGGTATA TCAACAAGCG CGACCCGAAC CAGGGGACCT GCACCAACGC CTGCCGCTGG GAATACAACG TGGCGGAAGG CAAAGAGGAC GACGTCGGCA ACATCGTCCA CAAATATGAG CCGATCCCGG TGCAGACCGT GGAGCCTACC CTCGGCATCG GCGCGCCGAC CGATAAAGTC TTTATGATTG AGGAAGCCAA ACGTCCGGGC GAATACATGA CCGCCTTTGA GGACGAACAC GGCACCTACA TCATGAACTC GAAAGATCTG CGGGCTATCG CCCACGTTGA GCGTCTGACC CAGATGGGCG TTCACTCGCT GAAGATCGAA GGCCGCACCA AATCCTTCTA CTACTGCGCG CGCACCGCTC AGGTTTACCG CAAGGCGATC GACGATGCCG CCGCCGGCAA ACCGTTCGAT ACCAGCCTGC TGGAGACCCT GGAGGGGCTG GCCCACCGCG GGTACACCGA AGGCTTCCTG CGCCGCCATA CCCATGACGA TTATCAGAAC TACGAGTACG GCTACTCCGT CTCCGAGCGC CAGCAGTTTG TCGGCGAATT CACCGGAGAG CGTAACGGCC CGCTGGCGGC CGTGGCGGTC AAAAATAAGT TCAGCGTCGG CGACAGCCTT GAGCTGATGA CCCCGCAGGG TAACGTCAAC TTCACCCTTG AGCATATGGA GAATGGCAAA GGCGAAACCA TGCCGGTGGC CCCGGGCGAT GGCTATACCG TCTGGTTGCC TGTGCCGGAC GACCTGCCCC TGCAGTATGC CCTGCTGATG CGCAACTTTA CTGGTCAAAC AACCAGAAAT CCGCACGGTA ACTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT