KlpnC.20453.a

Amine oxidase

CENTER ID: KlpnC.20453.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20453.a.B2.GE44420 Mature protein(KlpnC.20453.a) 32 755
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.2300
UniProt: A0A0H3GMJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANGLKFSPR KTALALAVAV VCAWQSPVFA HGSEAHMVPL DKTLQEFGAD VQWDDYAQMF TLIKDGAYVK VKPGAKTAIV NGKSLDLPVP VVMKEGKAWV SDTFINDVFQ SGLDQTFQVE KRPHPLNSLS AAEISEAVTI VKAAPEFQPN TRFTEISLHE PDKAAVWAFA LQGTPVDAPR TADVVMLDGK HVIEAVVDLQ NKKILSWTPI KGAHGMVLLD DFVSVQNIIN TSSEFAEVLK KHGITDPGKV VTTPLTVGFF DGKDGLQQDA RLLKVVSYLD TGDGNYWAHP IENLVAVVDL EAKKIIKIEE GPVIPVPMEP RPYDGRDRNA PAVKPLEITE PEGKNYTITG DTIHWQNWDF HLRLNSRVGP ILSTVTYNDN GTKRQVMYEG SLGGMIVPYG DPDVGWYFKA YLDSGDYGMG TLTSPIVRGK DAPSNAVLLD ETIADYTGKP TTIPGAVAIF ERYAGPEYKH LEMGKPNVST ERRELVVRWI STVGNYDYIF DWVFHDNGTI GIDAGATGIE AVKGVLAKTM HDPSAKEDTR YGTLIDHNIV GTTHQHIYNF RLDLDVDGEN NTLVAMDPEV KPNTAGGPRT STMQVNQYTI DSEQKAAQKF DPGTIRLLSN TSKENRMGNP VSYQIIPYAG GTHPAATGAK FAPDEWIYHR LSFMDKQLWV TRYHPTERYP EGKYPNRSAH DTGLGQYAKD DESLTNHDDV VWITTGTTHV ARAEEWPIMP TEWAHALLKP WNFFDETPTL GEKKK
NT Sequence
ATGGCAAACG GCTTGAAATT TTCTCCTCGT AAAACCGCGC TGGCGCTGGC CGTGGCGGTG GTTTGCGCCT GGCAATCACC GGTCTTCGCT CACGGTAGCG AAGCCCACAT GGTACCGCTG GATAAAACGC TGCAGGAATT CGGCGCTGAT GTGCAGTGGG ATGACTACGC GCAAATGTTC ACCCTGATAA AAGACGGCGC TTACGTCAAA GTAAAGCCCG GCGCCAAAAC GGCGATCGTC AACGGTAAAT CCCTTGACCT ACCGGTGCCT GTGGTAATGA AGGAAGGTAA AGCCTGGGTT TCCGATACCT TTATCAACGA TGTCTTCCAG TCCGGTCTCG ATCAGACCTT CCAGGTGGAA AAGCGCCCGC ACCCGTTAAA CTCGCTCTCG GCGGCGGAAA TCAGTGAAGC GGTGACCATT GTCAAAGCCG CGCCGGAGTT TCAGCCGAAT ACCCGCTTTA CTGAGATTTC CTTACACGAG CCGGACAAGG CGGCGGTATG GGCCTTTGCC CTGCAGGGAA CGCCGGTCGA TGCTCCCCGC ACTGCCGATG TGGTGATGCT CGATGGCAAA CACGTCATTG AAGCCGTGGT CGATCTGCAA AACAAAAAAA TCCTTTCCTG GACGCCGATC AAAGGCGCCC ACGGGATGGT GCTGCTCGAT GATTTTGTCA GCGTGCAGAA CATCATCAAT ACCAGCAGCG AATTCGCTGA GGTGCTGAAA AAGCACGGTA TTACCGATCC TGGCAAAGTG GTCACCACCC CGCTCACCGT TGGCTTTTTT GATGGCAAAG ATGGCCTGCA GCAGGATGCG CGTCTGCTGA AGGTGGTCAG TTATCTGGAT ACCGGCGACG GCAACTACTG GGCGCACCCG ATTGAAAACC TGGTGGCGGT GGTCGACCTT GAAGCGAAGA AAATCATCAA AATCGAAGAA GGTCCGGTGA TCCCGGTCCC AATGGAGCCC CGTCCGTATG ATGGTCGCGA CCGCAACGCC CCGGCGGTGA AACCGCTGGA GATAACCGAA CCGGAAGGCA AAAACTACAC CATTACCGGC GATACCATTC ACTGGCAGAA CTGGGATTTC CACCTGCGTC TTAACTCGCG CGTCGGACCA ATTCTGTCAA CGGTGACTTA CAACGATAAC GGCACAAAAC GCCAGGTGAT GTATGAAGGT TCCCTCGGCG GGATGATCGT CCCCTACGGC GACCCGGACG TCGGCTGGTA TTTCAAAGCC TACCTCGACT CCGGCGATTA CGGCATGGGC ACCCTGACGT CGCCGATTGT TCGCGGTAAA GATGCGCCGT CAAATGCGGT ACTGCTGGAC GAAACTATCG CCGATTACAC CGGCAAACCG ACCACGATTC CAGGCGCGGT GGCCATATTC GAACGCTATG CCGGGCCTGA GTATAAGCAC CTGGAGATGG GCAAACCCAA CGTCAGCACC GAACGCCGGG AACTGGTGGT ACGCTGGATC AGCACCGTCG GCAACTACGA CTATATCTTT GACTGGGTGT TCCACGACAA CGGCACCATC GGTATCGATG CCGGCGCCAC CGGCATTGAA GCGGTGAAAG GCGTGCTGGC GAAGACCATG CATGACCCCA GTGCCAAAGA GGATACCCGC TACGGAACGT TGATCGACCA TAATATTGTC GGCACCACTC ACCAGCACAT CTATAACTTC CGCCTCGACC TCGATGTGGA CGGCGAGAAC AACACCCTGG TGGCGATGGA TCCTGAAGTG AAGCCCAACA CCGCCGGCGG CCCGCGCACC AGCACCATGC AGGTGAATCA GTACACAATC GATAGCGAGC AGAAAGCGGC GCAGAAATTC GACCCGGGCA CTATCCGCCT GCTGAGCAAC ACCAGCAAAG AGAACCGCAT GGGCAACCCG GTCTCATACC AGATTATCCC TTACGCCGGC GGCACGCACC CGGCGGCGAC CGGTGCCAAA TTCGCCCCGG ACGAGTGGAT ATATCATCGC CTGAGCTTTA TGGATAAACA GCTGTGGGTG ACGCGCTACC ACCCGACAGA GCGATATCCG GAAGGGAAAT ACCCGAACCG TTCCGCCCAC GATACCGGCC TGGGCCAGTA CGCGAAGGAT GATGAGTCGC TGACTAACCA CGATGACGTC GTGTGGATCA CCACCGGCAC CACCCACGTG GCGCGCGCCG AAGAGTGGCC GATCATGCCG ACCGAGTGGG CGCACGCGCT GCTCAAGCCG TGGAACTTCT TTGACGAAAC CCCTACTCTG GGCGAAAAGA AAAAG
Details for KlpnC.20453.a.B2.GE44420
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 81kDa
OBSERVED MW: 81kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHGS EAHMVPLDKT LQEFGADVQW DDYAQMFTLI KDGAYVKVKP GAKTAIVNGK SLDLPVPVVM KEGKAWVSDT FINDVFQSGL DQTFQVEKRP HPLNSLSAAE ISEAVTIVKA APEFQPNTRF TEISLHEPDK AAVWAFALQG TPVDAPRTAD VVMLDGKHVI EAVVDLQNKK ILSWTPIKGA HGMVLLDDFV SVQNIINTSS EFAEVLKKHG ITDPGKVVTT PLTVGFFDGK DGLQQDARLL KVVSYLDTGD GNYWAHPIEN LVAVVDLEAK KIIKIEEGPV IPVPMEPRPY DGRDRNAPAV KPLEITEPEG KNYTITGDTI HWQNWDFHLR LNSRVGPILS TVTYNDNGTK RQVMYEGSLG GMIVPYGDPD VGWYFKAYLD SGDYGMGTLT SPIVRGKDAP SNAVLLDETI ADYTGKPTTI PGAVAIFERY AGPEYKHLEM GKPNVSTERR ELVVRWISTV GNYDYIFDWV FHDNGTIGID AGATGIEAVK GVLAKTMHDP SAKEDTRYGT LIDHNIVGTT HQHIYNFRLD LDVDGENNTL VAMDPEVKPN TAGGPRTSTM QVNQYTIDSE QKAAQKFDPG TIRLLSNTSK ENRMGNPVSY QIIPYAGGTH PAATGAKFAP DEWIYHRLSF MDKQLWVTRY HPTERYPEGK YPNRSAHDTG LGQYAKDDES LTNHDDVVWI TTGTTHVARA EEWPIMPTEW AHALLKPWNF FDETPTLGEK KK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggtagcg aagcccacat ggtaccgctg gataaaacgc tgcaggaatt cggcgctgat gtgcagtggg atgactacgc gcaaatgttc accctgataa aagacggcgc ttacgtcaaa gtaaagcccg gcgccaaaac ggcgatcgtc aacggtaaat cccttgacct accggtgcct gtggtaatga aggaaggtaa agcctgggtt tccgatacct ttatcaacga tgtcttccag tccggtctcg atcagacctt ccaggtggaa aagcgcccgc acccgttaaa ctcgctctcg gcggcggaaa tcagtgaagc ggtgaccatt gtcaaagccg cgccggagtt tcagccgaat acccgcttta ctgagatttc cttacacgag ccggacaagg cggcggtatg ggcctttgcc ctgcagggaa cgccggtcga tgctccccgc actgccgatg tggtgatgct cgatggcaaa cacgtcattg aagccgtggt cgatctgcaa aacaaaaaaa tcctttcctg gacgccgatc aaaggcgccc acgggatggt gctgctcgat gattttgtca gcgtgcagaa catcatcaat accagcagcg aattcgctga ggtgctgaaa aagcacggta ttaccgatcc tggcaaagtg gtcaccaccc cgctcaccgt tggctttttt gatggcaaag atggcctgca gcaggatgcg cgtctgctga aggtggtcag ttatctggat accggcgacg gcaactactg ggcgcacccg attgaaaacc tggtggcggt ggtcgacctt gaagcgaaga aaatcatcaa aatcgaagaa ggtccggtga tcccggtccc aatggagccc cgtccgtatg atggtcgcga ccgcaacgcc ccggcggtga aaccgctgga gataaccgaa ccggaaggca aaaactacac cattaccggc gataccattc actggcagaa ctgggatttc cacctgcgtc ttaactcgcg cgtcggacca attctgtcaa cggtgactta caacgataac ggcacaaaac gccaggtgat gtatgaaggt tccctcggcg ggatgatcgt cccctacggc gacccggacg tcggctggta tttcaaagcc tacctcgact ccggcgatta cggcatgggc accctgacgt cgccgattgt tcgcggtaaa gatgcgccgt caaatgcggt actgctggac gaaactatcg ccgattacac cggcaaaccg accacgattc caggcgcggt ggccatattc gaacgctatg ccgggcctga gtataagcac ctggagatgg gcaaacccaa cgtcagcacc gaacgccggg aactggtggt acgctggatc agcaccgtcg gcaactacga ctatatcttt gactgggtgt tccacgacaa cggcaccatc ggtatcgatg ccggcgccac cggcattgaa gcggtgaaag gcgtgctggc gaagaccatg catgacccca gtgccaaaga ggatacccgc tacggaacgt tgatcgacca taatattgtc ggcaccactc accagcacat ctataacttc cgcctcgacc tcgatgtgga cggcgagaac aacaccctgg tggcgatgga tcctgaagtg aagcccaaca ccgccggcgg cccgcgcacc agcaccatgc aggtgaatca gtacacaatc gatagcgagc agaaagcggc gcagaaattc gacccgggca ctatccgcct gctgagcaac accagcaaag agaaccgcat gggcaacccg gtctcatacc agattatccc ttacgccggc ggcacgcacc cggcggcgac cggtgccaaa ttcgccccgg acgagtggat atatcatcgc ctgagcttta tggataaaca gctgtgggtg acgcgctacc acccgacaga gcgatatccg gaagggaaat acccgaaccg ttccgcccac gataccggcc tgggccagta cgcgaaggat gatgagtcgc tgactaacca cgatgacgtc gtgtggatca ccaccggcac cacccacgtg gcgcgcgccg aagagtggcc gatcatgccg accgagtggg cgcacgcgct gctcaagccg tggaacttct ttgacgaaac ccctactctg ggcgaaaaga aaaagtgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat