SSGCID: Target Page

KlpnC.20452.a

Methylthioribose kinase

CENTER ID:	KlpnC.20452.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20452.a.B1.GE44419	Full length(KlpnC.20452.a)		1	399

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.1423
UniProt:	A0A0H3GPU2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQYHTFTAH DAVAYAQQFA GIDNPSELVS AQEVGDGNLN LVFKVFDRQG VSRAIVKQAL PYVRCVGESW PLTLDRARLE AQTLVAHYQH SPQHTVKIHH FDPELAVMVM EDLSDHRIWR GELIANVYYP QAARQLGDYL AQVLFHTSDF YLHPHEKKAQ VAQFINPAMC EITEDLFFND PYQIHERNNY PAELEADVAA LRDDAQLKLA VAALKHRFFA HAEALLHGDI HSGSIFVAEG SLKAIDAEFG YFGPIGFDIG TAIGNLLLNY CGLPGQLGIR DAAAAREQRL NDIHQLWTTF AERFQALAAE KTRDAALAYP GYASAFLKKV WADAVGFCGS ELIRRSVGLS HVADIDTIQD DAMRHECLRH AITLGRALIV LAERIDSVDE LLARVRQYS

NT Sequence

ATGTCGCAAT ACCATACCTT CACCGCCCAC GATGCCGTGG CTTACGCGCA ACAGTTCGCC GGCATCGACA ACCCATCTGA GCTGGTCAGC GCGCAGGAAG TGGGCGATGG CAACCTCAAT CTGGTGTTTA AAGTGTTCGA TCGTCAGGGC GTCAGCCGGG CGATCGTCAA ACAGGCCCTG CCCTACGTGC GCTGCGTCGG CGAATCCTGG CCGCTGACCC TCGACCGCGC CCGTCTCGAA GCGCAGACCC TGGTCGCCCA CTATCAGCAC AGCCCGCAGC ACACGGTAAA AATCCATCAC TTTGATCCCG AGCTGGCGGT GATGGTGATG GAAGATCTTT CCGACCACCG CATCTGGCGC GGAGAGCTTA TCGCTAACGT CTACTATCCC CAGGCGGCCC GCCAGCTTGG CGACTATCTG GCGCAGGTGT TGTTCCACAC CAGCGATTTC TACCTCCATC CCCACGAGAA AAAGGCGCAG GTGGCGCAGT TTATTAACCC GGCGATGTGC GAGATCACCG AGGATCTGTT CTTTAACGAC CCGTATCAGA TCCACGAGCG CAATAACTAC CCGGCGGAGC TGGAGGCCGA TGTCGCCGCC CTGCGCGACG ACGCCCAGCT TAAGCTGGCG GTGGCGGCGC TGAAGCACCG TTTCTTTGCC CATGCGGAAG CGCTGCTGCA CGGCGATATC CACAGCGGGT CGATCTTCGT TGCCGAAGGT AGCCTGAAGG CCATCGACGC CGAGTTCGGC TACTTCGGCC CCATCGGCTT CGATATCGGC ACCGCCATCG GCAACCTGCT GCTGAACTAC TGCGGCCTGC CGGGCCAGCT CGGCATTCGC GATGCCGCCG CCGCGCGCGA GCAGCGGCTG AACGACATCC ACCAGCTGTG GACCACCTTC GCCGAGCGCT TCCAGGCGCT GGCGGCGGAG AAAACCCGCG ACGCGGCGCT GGCTTACCCC GGCTACGCCT CCGCCTTTCT GAAGAAAGTC TGGGCGGACG CGGTCGGCTT CTGCGGCAGC GAACTGATCC GCCGCAGCGT CGGACTGTCG CACGTCGCGG ATATCGACAC TATCCAGGAC GACGCCATGC GTCATGAGTG CCTGCGCCAC GCCATTACCC TGGGCAGAGC GCTGATCGTG CTGGCCGAGC GTATCGACAG CGTCGACGAG CTGCTGGCGC GGGTACGCCA GTACAGC

Details for KlpnC.20452.a.B1.GE44419

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMS QYHTFTAHDA VAYAQQFAGI DNPSELVSAQ EVGDGNLNLV FKVFDRQGVS RAIVKQALPY VRCVGESWPL TLDRARLEAQ TLVAHYQHSP QHTVKIHHFD PELAVMVMED LSDHRIWRGE LIANVYYPQA ARQLGDYLAQ VLFHTSDFYL HPHEKKAQVA QFINPAMCEI TEDLFFNDPY QIHERNNYPA ELEADVAALR DDAQLKLAVA ALKHRFFAHA EALLHGDIHS GSIFVAEGSL KAIDAEFGYF GPIGFDIGTA IGNLLLNYCG LPGQLGIRDA AAAREQRLND IHQLWTTFAE RFQALAAEKT RDAALAYPGY ASAFLKKVWA DAVGFCGSEL IRRSVGLSHV ADIDTIQDDA MRHECLRHAI TLGRALIVLA ERIDSVDE

Validated NT Sequence

agctcgtcga cgctgtcgat acgctcggcc agcacgatca gcgctctgcc cagggtaatg gcgtggcgca ggcactcatg acgcatggcg tcgtcctgga tagtgtcgat atccgcgacg tgcgacagtc cgacgctgcg gcggatcagt tcgctgccgc agaagccgac cgcgtccgcc cacacctttt tcagaaaggc ggaggcgtag ccggggtaag ccagcgccgc gtcgcgggtt ttctccgccg ccagcgcctg gaagcgctcg gcaaaggtgg tccacagctg gtggatgtcg ttcagccgct gctcgcgcgc ggcggcggca tcgcgaatgc cgagctggcc cggcaggccg cagtagttca gcagcaggtt gccgatggcg gtgccgatat cgaagccaat ggggccgaag tagccgaact cggcgtcgat ggccttcagg ctgccttcgg caacgaagat cgacccgctg tggatatcgc cgtgcagcag cgcttccgca tgggcaaaga aacggtgctt cagcgccgcc accgccagct taagctgggc gtcgtcgcgc agggcggcga catcggcctc cagctccgcc gggtagttat tgcgctcgtg gatctgatac gggtcgttaa agaacagatc ctcggtgatc tcgcacatcg ccgggttaat aaactgcgcc acctgcgcct ttttctcgtg gggatggagg tagaaatcgc tggtgtgaaa cagcacctgc gccagatagt cgccaagctg gcgggccgcc tggggatagt agacgttagc gataagctct ccgcgccaga tgcggtggtc ggaaagatct tccatcacca tcaccgccag ctcgggatca aagtgatgga tttttaccgt gtgctgcggg ctgtgctgat agtgggcgac cagggtctgc gcttcgagac gggcgcggtc gagggtcagc ggccaggatt cgccgacgca gcgcacgtag ggcagggcct gtttgacgat cgcccggctg acgccctgac gatcgaacac tttaaacacc agattgaggt tgccatcgcc cacttcctgc gcgctgacca gctcagatgg gttgtcgatg ccggcgaact gttgcgcgta agccacggca tcgtgggcgg tgaaggtatg gtattgcgac atatggtggt ggtggtggtg agccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcgc aataccatac cttcaccgcc cacgatgccg tggcttacgc gcaacagttc gccggcatcg acaacccatc tgagctggtc agcgcgcagg aagtgggcga tggcaacctc aatctggtgt ttaaagtgtt cgatcgtcag ggcgtcagcc gggcgatcgt caaacaggcc ctgccctacg tgcgctgcgt cggcgaatcc tggccgctga ccctcgaccg cgcccgtctc gaagcgcaga ccctggtcgc ccactatcag cacagcccgc agcacacggt aaaaatccat cactttgatc ccgagctggc ggtgatggtg atggaagatc tttccgacca ccgcatctgg cgcggagagc ttatcgctaa cgtctactat ccccaggcgg cccgccagct tggcgactat ctggcgcagg tgttgttcca caccagcgat ttctacctcc atccccacga gaaaaaggcg caggtggcgc agtttattaa cccggcgatg tgcgagatca ccgaggatct gttctttaac gacccgtatc agatccacga gcgcaataac tacccggcgg agctggaggc cgatgtcgcc gccctgcgcg acgacgccca gcttaagctg gcggtggcgg cgctgaagca ccgtttcttt gcccatgcgg aagcgctgct gcacggcgat atccacagcg ggtcgatctt cgttgccgaa ggtagcctga aggccatcga cgccgagttc ggctacttcg gccccatcgg cttcgatatc ggcaccgcca tcggcaacct gctgctgaac tactgcggcc tgccgggcca gctcggcatt cgcgatgccg ccgccgcgcg cgagcagcgg ctgaacgaca tccaccagct gtggaccacc ttcgccgagc gcttccaggc gctggcggcg gagaaaaccc gcgacgcggc gctggcttac cccggctacg cctccgcctt tctgaagaaa gtctgggcgg acgcggtcgg cttctgcggc agcgaactga tccgccgcag cgtcggactg tcgcacgtcg cggatatcga cactatccag gacgacgcca tgcgtcatga gtgcctgcgc cacgccatta ccctgggcag agcgctgatc gtgctggccg agcgtatcga cagcgtcgac gagctgctgg cgcgggtacg ccagtacagc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat