KlpnC.20451.a

Iron-enterobactin transporter periplasmic binding protein

CENTER ID: KlpnC.20451.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20451.a.B2.GE44418 Mature protein(KlpnC.20451.a) 26 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1125630.4.peg.1405
UniProt: A0A0H3GL81

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNFFSFCRRG ALTGMLLLLG ITSAQAADWP RQVTDSYGTH TLPSQPLRIV STSVTLTGSL LAIDAPVVAS GATTPNNRVA DSQGFLRQWS EVAKARKLAR LYIGEPSAEA VAAQMPDLIL VSATGGDSAL PLYDQLKTIA PTLVINYDDK SWQTLLTQLG QITGHEQQAS ARIADFNKQL VSLKEKMKLP PQPVTALVYT AAAHSANIWT PESAQGQMLQ QLGFSLATLP GGLPASHSQG KRHDIVQLGG ENLAAGLNGQ SLFLFAGDQK DADAIYANPL LAHLPAVAGK RVYPLGTETF RLDYYSALLV LQRLSSLFG
NT Sequence
GTGAACTTCT TTTCTTTCTG CCGCCGCGGT GCGCTGACCG GCATGCTGTT GCTTTTAGGA ATTACCTCAG CCCAGGCGGC GGACTGGCCG CGCCAGGTCA CCGACAGCTA CGGGACGCAT ACCCTGCCGA GTCAGCCGCT GCGCATTGTC TCCACCAGCG TGACGCTTAC CGGTTCGCTG CTGGCGATTG ACGCTCCGGT GGTCGCCAGC GGCGCGACCA CGCCGAACAA CCGCGTCGCC GATAGCCAGG GCTTTCTGCG CCAGTGGAGC GAGGTTGCCA AAGCCCGCAA GCTGGCGCGG CTGTACATCG GCGAACCAAG CGCCGAAGCG GTGGCCGCCC AGATGCCGGA TCTGATCCTG GTGAGCGCGA CCGGCGGCGA CTCCGCCCTG CCGCTGTACG ATCAGCTTAA AACCATCGCC CCGACGCTGG TCATCAACTA TGACGATAAA AGCTGGCAAA CCCTGCTCAC CCAGCTGGGG CAGATCACCG GTCATGAACA ACAGGCCAGC GCGCGCATTG CCGACTTCAA TAAACAGCTC GTTTCGTTAA AAGAAAAAAT GAAACTGCCG CCGCAGCCGG TGACCGCCCT GGTCTATACC GCCGCCGCGC ACAGCGCCAA TATCTGGACG CCGGAATCGG CTCAGGGGCA GATGCTTCAA CAGCTGGGCT TTAGCCTGGC GACGCTGCCG GGCGGCCTGC CCGCCAGCCA TAGCCAGGGT AAACGCCACG ATATCGTGCA GCTGGGCGGG GAGAACCTCG CGGCCGGTCT GAACGGCCAG AGCCTGTTCC TGTTCGCCGG CGACCAGAAA GATGCGGATG CCATCTACGC CAACCCGCTG CTGGCGCATC TCCCGGCGGT CGCAGGCAAA CGCGTCTACC CGCTGGGCAC CGAGACCTTC CGCCTTGACT ACTACAGCGC TCTGCTGGTG CTCCAGCGTC TCTCCAGCCT GTTCGGC
Details for KlpnC.20451.a.B2.GE44418
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA ADWPRQVTDS YGTHTLPSQP LRIVSTSVTL TGSLLAIDAP VVASGATTPN NRVADSQGFL RQWSEVAKAR KLARLYIGEP SAEAVAAQMP DLILVSATGG DSALPLYDQL KTIAPTLVIN YDDKSWQTLL TQLGQITGHE QQASARIADF NKQLVSLKEK MKLPPQPVTA LVYTAAAHSA NIWTPESTQG QMLEQLGFSL ATLPGGLPAS HSQGKRHDIV QLGGENLAAG LNGQSLFLFA GDQKDADAIY ANPLLAHLPA VAGKRVYPLG TETFRLDYYS ALLVLQRLSS LFG
Validated NT Sequence
tactcactta gccgaacagg ctggagagac gctggagcac cagcagagcg ctgtagtagt caaggcggaa ggtctcggtg cccagcgggt agacgcgttt gcctgcgacc gccgggagat gcgccagcag cgggttggcg tagatggcat ccgcatcttt ctggtcgccg gcgaacagga acagactctg gccgttcaga ccggccgcga ggttctcccc gcccagctgc acgatatcgt ggcgtttacc ctggctatgg ctggcgggca ggccgcccgg cagcgtcgcc aggctaaagc ccagctgttc gagcatctgc ccctgagtcg attccggcgt ccagatattg gcgctgtgcg cggcggcggt atagaccagg gcggtcaccg gctgcggcgg cagtttcatt ttttctttta acgaaacgag ctgtttattg aagtcggcaa tgcgcgcgct ggcctgttgt tcatgaccgg tgatctgccc cagctgggtg agcagggttt gccagctttt atcgtcatag ttgatgacca gcgtcggggc gatggtttta agctgatcgt acagcggcag ggcggagtcg ccaccggtcg cgctcaccag gatcagatcc ggcatctggg cggccaccgc ttcggcgctt ggttcgccga tgtacagccg cgccagcttg cgggctttgg caacctcgct ccactggcgc agaaagccct ggctatcggc gacgcggttg ttcggcgtgg tcgcgccgct ggcgaccacc ggagcgtcaa tcgccagcag cgaaccggta agcgtcacgc tggtggagac aatgcgcagc ggctgactcg gcagggtatg cgtcccgtag ctgtcggtga cctggcgcgg ccagtccgcc gccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHAA DWPRQVTDSY GTHTLPSQPL RIVSTSVTLT GSLLAIDAPV VASGATTPNN RVADSQGFLR QWSEVAKARK LARLYIGEPS AEAVAAQMPD LILVSATGGD SALPLYDQLK TIAPTLVINY DDKSWQTLLT QLGQITGHEQ QASARIADFN KQLVSLKEKM KLPPQPVTAL VYTAAAHSAN IWTPESAQGQ MLQQLGFSLA TLPGGLPASH SQGKRHDIVQ LGGENLAAGL NGQSLFLFAG DQKDADAIYA NPLLAHLPAV AGKRVYPLGT ETFRLDYYSA LLVLQRLSSL FG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcggcgg actggccgcg ccaggtcacc gacagctacg ggacgcatac cctgccgagt cagccgctgc gcattgtctc caccagcgtg acgcttaccg gttcgctgct ggcgattgac gctccggtgg tcgccagcgg cgcgaccacg ccgaacaacc gcgtcgccga tagccagggc tttctgcgcc agtggagcga ggttgccaaa gcccgcaagc tggcgcggct gtacatcggc gaaccaagcg ccgaagcggt ggccgcccag atgccggatc tgatcctggt gagcgcgacc ggcggcgact ccgccctgcc gctgtacgat cagcttaaaa ccatcgcccc gacgctggtc atcaactatg acgataaaag ctggcaaacc ctgctcaccc agctggggca gatcaccggt catgaacaac aggccagcgc gcgcattgcc gacttcaata aacagctcgt ttcgttaaaa gaaaaaatga aactgccgcc gcagccggtg accgccctgg tctataccgc cgccgcgcac agcgccaata tctggacgcc ggaatcggct caggggcaga tgcttcaaca gctgggcttt agcctggcga cgctgccggg cggcctgccc gccagccata gccagggtaa acgccacgat atcgtgcagc tgggcgggga gaacctcgcg gccggtctga acggccagag cctgttcctg ttcgccggcg accagaaaga tgcggatgcc atctacgcca acccgctgct ggcgcatctc ccggcggtcg caggcaaacg cgtctacccg ctgggcaccg agaccttccg ccttgactac tacagcgctc tgctggtgct ccagcgtctc tccagcctgt tcggctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat