KlpnC.20450.a

Probable phosphatase KPHS_19330

CENTER ID: KlpnC.20450.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20450.a.B1.GE44417 Full length(KlpnC.20450.a) 1 245
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.1883
UniProt: A0A0H3GRB9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MYPVDLHMHT VASTHAYSTL HDYIAEAKRK GIKLFAITDH GPDMADAPHY WHFVNMRIWP RLVDGVGILR GIESNIKNIE GEIDCSGPML TSLDLIIAGF HEPVFPPQDR DTHTQAMIAA MASGKVHMIS HPGNPKFPVD IPAIAEAAAR YQVALEINNS SFVSSRVGSE DNCRAIAAAV RDAGGWVALG SDSHTAFTLG EFTECRKILD AVDFPEERIL NVSPCRLLNF LESRGMPAIP EFADL
NT Sequence
ATGTATCCCG TTGACCTGCA CATGCATACC GTCGCCAGCA CCCATGCCTA CAGCACCCTG CACGATTATA TTGCGGAAGC AAAGCGTAAA GGGATCAAAC TCTTCGCCAT CACCGACCAC GGCCCGGACA TGGCGGATGC TCCGCACTAC TGGCACTTCG TTAATATGCG CATCTGGCCC CGCCTGGTAG ACGGGGTGGG GATCCTGCGC GGTATTGAGT CGAATATTAA AAATATTGAA GGCGAGATCG ACTGTTCCGG ACCGATGTTG ACTTCTCTCG ATCTGATCAT CGCCGGATTT CATGAGCCGG TGTTTCCGCC GCAGGATCGT GACACCCATA CACAGGCGAT GATAGCCGCG ATGGCCAGCG GTAAAGTGCA TATGATTAGC CATCCGGGTA ATCCGAAATT CCCGGTGGAT ATCCCGGCGA TTGCCGAAGC GGCGGCTCGC TACCAGGTGG CGCTGGAGAT TAATAACTCC TCGTTCGTAT CATCGCGCGT GGGCAGTGAA GACAACTGCC GGGCCATCGC CGCCGCAGTG CGTGATGCCG GGGGATGGGT GGCGCTGGGC TCGGACTCTC ACACCGCCTT TACCCTTGGG GAATTTACCG AATGCCGCAA GATCCTTGAT GCGGTCGATT TCCCGGAGGA GAGGATCCTC AACGTTTCTC CGTGTCGCCT GCTTAACTTC CTCGAGTCGC GGGGCATGCC GGCGATCCCG GAATTTGCTG ACCTT
Details for KlpnC.20450.a.B1.GE44417
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 28kDa
OBSERVED MW: 28kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMY PVDLHMHTVA STHAYSTLHD YIAEAKRKGI KLFAITDHGP DMADAPHYWH FVNMRIWPRL VDGVGILRGI ESNIKNIEGE IDCSGPMLTS LDLIIAGFHE PVFPPQDRDT HTQAMIAAMA SGKVHMISHP GNPKFPVDIP AIAEAAARYQ VALEINNSSF VSSRMGSEDN CRAIAAAVRD AGGWVALGSD SHTAFTLGEF TECRKILDAV DFPEERILNV SPRRLLNFLE SRGMPAIPEF ADL
Validated NT Sequence
atcttactca cttaaaggtc agcaaattcc gggatcgccg gcatgccccg cgactcgagg aagttaagca ggcgacgcgg agaaacgttg aggatcctct cctccgggaa atcgaccgca tcaaggatct tgcggcattc ggtaaattcc ccgagggtaa aggcggtgtg agagtccgag cccagcgcca cccatccccc ggcatcacgc actgcggcgg cgatggcccg gcagttgtct tcactgccca tgcgcgatga tacgaacgag gagttattaa tctccagcgc cacctggtag cgagccgccg cttcggcaat ggccgggata tccaccggga atttcggatt acccggatgg ctaatcatat gcactttacc gctggccatc gcggctatca tcgcctgtgt atgggtgtca cgatcctgcg gcggaaacac cggctcatga aatccggcga tgatcagatc gagggaagtc aacatcggtc cggaacagtc gatctcgcct tcaatatttt taatattcga ctcaataccg cgcaggatcc ccaccccgtc taccaggcgg ggccagatgc gcatattaac gaagtgccag tagtgcggag catccgccat gtccgggccg tggtcggtga tggcgaagag tttgatccct ttacgctttg cttccgcaat ataatcgtgc agggtgctgt aggcatgggt gctggcgacg gtatgcatgt gcaggtcaac gggatacata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMY PVDLHMHTVA STHAYSTLHD YIAEAKRKGI KLFAITDHGP DMADAPHYWH FVNMRIWPRL VDGVGILRGI ESNIKNIEGE IDCSGPMLTS LDLIIAGFHE PVFPPQDRDT HTQAMIAAMA SGKVHMISHP GNPKFPVDIP AIAEAAARYQ VALEINNSSF VSSRVGSEDN CRAIAAAVRD AGGWVALGSD SHTAFTLGEF TECRKILDAV DFPEERILNV SPCRLLNFLE SRGMPAIPEF ADL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtatc ccgttgacct gcacatgcat accgtcgcca gcacccatgc ctacagcacc ctgcacgatt atattgcgga agcaaagcgt aaagggatca aactcttcgc catcaccgac cacggcccgg acatggcgga tgctccgcac tactggcact tcgttaatat gcgcatctgg ccccgcctgg tagacggggt ggggatcctg cgcggtattg agtcgaatat taaaaatatt gaaggcgaga tcgactgttc cggaccgatg ttgacttctc tcgatctgat catcgccgga tttcatgagc cggtgtttcc gccgcaggat cgtgacaccc atacacaggc gatgatagcc gcgatggcca gcggtaaagt gcatatgatt agccatccgg gtaatccgaa attcccggtg gatatcccgg cgattgccga agcggcggct cgctaccagg tggcgctgga gattaataac tcctcgttcg tatcatcgcg cgtgggcagt gaagacaact gccgggccat cgccgccgca gtgcgtgatg ccgggggatg ggtggcgctg ggctcggact ctcacaccgc ctttaccctt ggggaattta ccgaatgccg caagatcctt gatgcggtcg atttcccgga ggagaggatc ctcaacgttt ctccgtgtcg cctgcttaac ttcctcgagt cgcggggcat gccggcgatc ccggaatttg ctgacctttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat