KlpnC.20449.a

Siroheme synthase

CENTER ID: KlpnC.20449.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20449.a.B1.GE44416 Full length(KlpnC.20449.a) 1 457
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4792
UniProt: A0A0H3H3Z9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDHLPIFCQL RQRDCLLVGG GDVAERKARL LLDAGANVTV NALDFTPQFQ VWADSQMLTL VQGEFIPSLL DNCWLAIAAT DDETVNQQVS EAAEARRIFC NVVDAPRQAS FIMPSIIDRS PLMVAVSSGG TSPVLARLLR EKLESILPLH LGQLARYAGH LRARVKQQFA TVGERRRFWE KLFVNDRLAQ SLANDDRQAV ADTTEQLLTE PLEHRGEVVL VGAGPGDAGL LTLKGLQQIQ QADVVVYDRL VSDDIMNLVR RDADRVFVGK RSGYHCVPQE EINQILLREA QKGRRVVRLK GGDPFIFGRG GEELETLCEA GIPFSVVPGI TAASGCSAYS GIPLTHRDFA QGVRLVTGHL KTGGELDWAN LAVEKQTLVF YMGLNQAPAI REKLIAHGMA EDMPAAIVEN GTAVTQKVVS GTLGQLDILA QQMASPALII VGRVVGLRDK LNWFSNH
NT Sequence
GTGGATCACT TACCCATATT CTGCCAGTTG CGCCAGCGCG ATTGTTTACT GGTTGGCGGC GGGGATGTCG CCGAACGCAA AGCCCGTCTG CTGCTGGATG CTGGCGCTAA CGTCACGGTC AATGCGCTCG ACTTCACGCC CCAGTTTCAG GTGTGGGCTG ATTCACAGAT GCTAACTCTG GTGCAGGGGG AGTTTATCCC CTCGCTGCTG GATAACTGCT GGCTGGCCAT CGCGGCTACT GATGATGAGA CGGTCAACCA GCAGGTTAGC GAGGCGGCCG AAGCGCGGCG TATCTTCTGT AACGTGGTGG ATGCCCCGCG CCAGGCCAGC TTTATCATGC CGTCGATAAT CGATCGCTCG CCGCTGATGG TCGCCGTCTC CTCGGGCGGG ACATCACCGG TTCTGGCGCG CCTGCTGCGC GAGAAACTGG AGTCAATTCT GCCCCTGCAT CTCGGCCAGC TGGCCCGCTA TGCCGGCCAT CTGCGCGCGC GGGTTAAGCA GCAGTTTGCC ACCGTAGGCG AGCGTCGCCG CTTCTGGGAG AAGCTGTTTG TTAACGACCG TCTGGCGCAG TCGCTGGCTA ATGACGATCG GCAGGCAGTC GCGGATACCA CTGAGCAGTT ACTGACGGAG CCGTTGGAGC ATCGCGGCGA GGTGGTGCTG GTTGGCGCCG GCCCCGGTGA CGCCGGGCTG CTCACGCTCA AAGGCCTGCA GCAGATCCAG CAGGCTGACG TGGTGGTCTA TGATCGGCTG GTCTCCGATG ACATCATGAA TCTGGTGCGC CGCGATGCCG ATCGGGTATT CGTCGGCAAG CGCTCAGGCT ACCACTGCGT CCCGCAGGAG GAGATCAACC AGATCCTGCT GCGCGAAGCG CAAAAAGGAA GACGCGTGGT CCGGCTGAAA GGCGGCGATC CCTTTATCTT TGGCCGCGGG GGGGAAGAGC TGGAGACGCT TTGCGAGGCG GGCATCCCCT TCTCCGTTGT GCCTGGCATT ACCGCCGCCT CCGGCTGCTC CGCTTATTCG GGGATCCCGT TAACACATCG CGATTTCGCC CAGGGCGTCC GCCTGGTCAC CGGCCACCTG AAAACCGGCG GCGAGCTGGA CTGGGCGAAC CTGGCGGTGG AGAAGCAAAC CCTGGTGTTC TACATGGGTC TGAACCAGGC GCCGGCGATC CGCGAGAAGC TTATCGCTCA CGGCATGGCT GAAGATATGC CCGCCGCCAT CGTCGAGAAC GGCACCGCTG TGACGCAAAA AGTGGTCAGC GGCACCTTAG GACAACTGGA TATTCTGGCG CAGCAGATGG CCAGCCCGGC GCTGATCATC GTCGGCCGCG TGGTCGGTCT GCGGGATAAA CTGAACTGGT TTTCGAACCA T
Details for KlpnC.20449.a.B1.GE44416
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMV DHLPIFCQLR QRDCLLVGGG DVAERKARLL LDAGANVTVN ALDFTPQFQV WADSQMLTLV QGEFIPSLLD NCWLAIAATD DETVNQQVSE AAEARRIFCN VVDAPRQASF IMPSIIDRSP LMVAVSSGGT SPVLARLLRE KLESILPLHL GQLARYAGHL RARVKQQFAT VGERRRFWEK LFVNDRLAQS LANDDRQAVA DTTEQLLTEP LEHRGEVVLV GAGPGDAGLL TLKGLQQIQQ ADVVVYDRLV SDDIMNLVRR DADRVFVGKR SGYHCVPQEE INQILLREAQ KGRRVVRLKG GDPFIFGRGG EELETLCEAG IPFSVVPGIT AASGCSAYSG IPLTHRDFAQ GVRLVTGHLK TGGELDWANL AVEKQTLVFY MGLNQAPAIR EKLIAHGMAE DMPAAIVENG TAVTQKVVSG TLGQLDILAQ QMASPALIIV GRVVGLRD
Validated NT Sequence
tttatcccgc agaccgacca cgcggccaac gatgatcagc gccgggctgg ccatctgctg cgccagaata tccagttgtc ctaaggtgcc gctgaccact ttttgcgtca cagcggtgcc gttctcgacg atggcggcgg gcatatcttc agccatgccg tgagcgataa gcttctcgcg gatcgccggc gcctggttca gacccatgta gaacaccagg gtttgcttct ccaccgccag gttcgcccag tccagctcgc cgccggtttt caggtggcca gtgaccaggc ggacgccctg cgcgaaatcg cggtgggtca aggggatccc cgaataagcg gagcagccgg aggcggcggt aatgccaggc acaacggaga aggggatgcc cgcctcgcaa agcgtctcca gctcttcccc cccgcggcca aagataaagg gatcgccgcc tttcagccgg accacgcgtc ttcctttttg cgcttcgcgc agcaggatct ggttgatctc ctcctgcggg acgcagtggt agcctgagcg cttgccgacg aatacccgat cggcatcgcg gcgcaccaga ttcatgatgt catcggagac cagccgatca tagaccacca cgtcagcctg ctggatctgc tgcaggcctt tgagcgtgag cagcccggcg tcaccggggc cggcgccaac cagcaccacc tcgccgcgat gctccaacgg ctccgtcagc aactgctcag tggtatccgc gactgcctgc cgatcgtcat tagccagcga ctgcgccaga cggtcgttaa caaacagctt ctcccagaag cggcgacgct cgcctacggt ggcaaactgc tgcttaaccc gcgcgcgcag atggccggca tagcgggcca gctggccgag atgcaggggc agaattgact ccagtttctc gcgcagcagg cgcgccagaa ccggtgatgt cccgcccgag gagacggcga ccatcagcgg cgagcgatcg atgatcgacg gcatgataaa gctggcctgg cgcggggcat ccaccacgtt acagaagata cgccgcgctt cggccgcctc gctaacctgc tggttgaccg tctcatcatc agtagcggcg atggccagcc agcagttatc cagcagcgag gggataaact ccccctgcac cagagttagc atctgtgaat cagcccacac ctgaaactgg ggcgtgaagt cgagcgcatt gaccgtgacg ttagcgccag catccagcag cagacgggct ttgcgttcgg cgacatcccc gccgccaacc agtaaacaat cgcgctggcg caactggcag aatatgggta agtgatccac catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMD HLPIFCQLRQ RDCLLVGGGD VAERKARLLL DAGANVTVNA LDFTPQFQVW ADSQMLTLVQ GEFIPSLLDN CWLAIAATDD ETVNQQVSEA AEARRIFCNV VDAPRQASFI MPSIIDRSPL MVAVSSGGTS PVLARLLREK LESILPLHLG QLARYAGHLR ARVKQQFATV GERRRFWEKL FVNDRLAQSL ANDDRQAVAD TTEQLLTEPL EHRGEVVLVG AGPGDAGLLT LKGLQQIQQA DVVVYDRLVS DDIMNLVRRD ADRVFVGKRS GYHCVPQEEI NQILLREAQK GRRVVRLKGG DPFIFGRGGE ELETLCEAGI PFSVVPGITA ASGCSAYSGI PLTHRDFAQG VRLVTGHLKT GGELDWANLA VEKQTLVFYM GLNQAPAIRE KLIAHGMAED MPAAIVENGT AVTQKVVSGT LGQLDILAQQ MASPALIIVG RVVGLRDKLN WFSNH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggatc acttacccat attctgccag ttgcgccagc gcgattgttt actggttggc ggcggggatg tcgccgaacg caaagcccgt ctgctgctgg atgctggcgc taacgtcacg gtcaatgcgc tcgacttcac gccccagttt caggtgtggg ctgattcaca gatgctaact ctggtgcagg gggagtttat cccctcgctg ctggataact gctggctggc catcgcggct actgatgatg agacggtcaa ccagcaggtt agcgaggcgg ccgaagcgcg gcgtatcttc tgtaacgtgg tggatgcccc gcgccaggcc agctttatca tgccgtcgat aatcgatcgc tcgccgctga tggtcgccgt ctcctcgggc gggacatcac cggttctggc gcgcctgctg cgcgagaaac tggagtcaat tctgcccctg catctcggcc agctggcccg ctatgccggc catctgcgcg cgcgggttaa gcagcagttt gccaccgtag gcgagcgtcg ccgcttctgg gagaagctgt ttgttaacga ccgtctggcg cagtcgctgg ctaatgacga tcggcaggca gtcgcggata ccactgagca gttactgacg gagccgttgg agcatcgcgg cgaggtggtg ctggttggcg ccggccccgg tgacgccggg ctgctcacgc tcaaaggcct gcagcagatc cagcaggctg acgtggtggt ctatgatcgg ctggtctccg atgacatcat gaatctggtg cgccgcgatg ccgatcgggt attcgtcggc aagcgctcag gctaccactg cgtcccgcag gaggagatca accagatcct gctgcgcgaa gcgcaaaaag gaagacgcgt ggtccggctg aaaggcggcg atccctttat ctttggccgc gggggggaag agctggagac gctttgcgag gcgggcatcc ccttctccgt tgtgcctggc attaccgccg cctccggctg ctccgcttat tcggggatcc cgttaacaca tcgcgatttc gcccagggcg tccgcctggt caccggccac ctgaaaaccg gcggcgagct ggactgggcg aacctggcgg tggagaagca aaccctggtg ttctacatgg gtctgaacca ggcgccggcg atccgcgaga agcttatcgc tcacggcatg gctgaagata tgcccgccgc catcgtcgag aacggcaccg ctgtgacgca aaaagtggtc agcggcacct taggacaact ggatattctg gcgcagcaga tggccagccc ggcgctgatc atcgtcggcc gcgtggtcgg tctgcgggat aaactgaact ggttttcgaa ccattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat