SSGCID: Target Page

KlpnC.20443.a

Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

CENTER ID:	KlpnC.20443.a
ORGANISM:	Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	unclassified

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20443.a.B1.GE44410	Full length(KlpnC.20443.a)		1	351

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
KlpnC.20443.a.B1.PW39061	Full length(KlpnC.20443.a)		1	351

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|1125630.4.peg.4146
UniProt:	A0A0H3H267

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQNLNTVLRA IPAPDADAMA RAQQHIDGLL KPPGSLGRLE ALAVQLAGMP GLGGQPQVAK KALLVMCADH GVWDEGVAIS PKAVTAIQAA NMTRGTTGVC VLAAQAGAKV HVIDVGIDSE PLPGVVNMRV ARGCGNIARG PAMSREQGQE LLLEVMRYTR ALAQEGVTLF GVGELGMANT TPAAAIVSVL TGSDAQEVVG IGANLPLAKV GNKMEVVRRA IAVNQPDPND GLDVLSKVGG FDLLGMTGVM LGAASCGLPV VLDGFLSYAA ALAACQIAPE VKPYLIPSHY SAEKGARIAL AHLGLEPYLN MGMRLGEGSG AALAMPIVEA ACAMYHRMGM LAASNIVLPK G

NT Sequence

ATGCAAAATT TAAACACGGT TCTGCGGGCG ATCCCCGCGC CGGACGCCGA CGCCATGGCG CGGGCACAGC AACACATCGA CGGCTTACTC AAGCCCCCCG GCAGCCTCGG GCGTCTGGAG GCGCTGGCGG TGCAGCTGGC GGGAATGCCC GGCCTGGGCG GCCAGCCGCA GGTGGCGAAA AAGGCCTTGC TGGTGATGTG CGCCGACCAT GGAGTGTGGG ACGAAGGGGT GGCGATTTCG CCGAAGGCGG TCACCGCCAT CCAGGCGGCA AATATGACCC GCGGCACCAC CGGCGTCTGC GTGCTCGCCG CCCAGGCCGG GGCGAAAGTG CATGTAATTG ATGTCGGTAT CGATAGCGAA CCGCTGCCCG GCGTGGTGAA TATGCGGGTG GCGCGGGGCT GCGGCAATAT TGCCCGCGGT CCGGCGATGA GCCGTGAGCA GGGGCAGGAG CTGTTGCTGG AGGTGATGCG CTACACCCGC GCGCTGGCCC AGGAGGGCGT TACCCTGTTT GGCGTCGGCG AACTGGGGAT GGCCAATACC ACGCCGGCGG CAGCGATCGT CAGCGTCCTG ACCGGCAGCG ATGCGCAGGA GGTGGTCGGG ATTGGCGCCA ATCTGCCGCT GGCGAAGGTT GGCAACAAGA TGGAGGTGGT GCGGCGGGCT ATCGCCGTCA ATCAGCCCGA CCCGAATGAT GGCCTCGACG TACTGAGCAA AGTCGGTGGT TTCGATCTGC TGGGGATGAC CGGTGTCATG CTGGGGGCGG CGTCCTGTGG GCTGCCGGTG GTGCTGGATG GCTTTCTCTC CTACGCGGCG GCGCTGGCGG CGTGTCAGAT AGCGCCGGAG GTGAAACCGT ATCTGATCCC GTCCCACTAT TCGGCGGAGA AGGGGGCGCG TATCGCGTTG GCGCACCTGG GCCTCGAACC GTATCTCAAT ATGGGCATGC GTCTGGGAGA AGGCAGCGGC GCGGCGCTGG CGATGCCGAT CGTCGAGGCG GCCTGCGCGA TGTACCATCG CATGGGCATG CTGGCGGCAA GCAATATTGT GTTACCGAAG GGG

Details for KlpnC.20443.a.B1.GE44410

HARVESTED ON:	6/30/2021
SEQUENCED ON:	7/22/2021
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ NLNTVLRAIP APDADAMARA QQHIDGLLKP PGSLGRLEAL AVQLAGMPGL GGQPQVAKKA LLVMCADHGV WDEGVAISPK AVTAIQAANM TRGTTGVCVL AAQAGAKVHV IDVGIDSVPL PGVVNMRVAR GCGNIARGPA MSREQGQELL LEVMRYTRAL AQEGVTLFGV GELGMANTTP AAAIVSVLTG SDAQEVVGIG ANLPLAKVGN KVEVVRRAIA VNQPDPNDGL DVLSKVGGFD LLGMTGVMLG AASCGLPVVL DGFLSYAAAL AACQIAPEVK PYLIPSHYSA EKGARIALAH LGLEPYLNMG MRLGEGSGAA LAMPIVEAAC AMYHRMGMLA A

Validated NT Sequence

gcttgccgcc agcatgccca tgcgatggta catcgcgcag gccgcctcga cgatcggcat cgccagcgcc gcgccgctgc cttctcccag acgcatgccc atattgagat acggttcgag acccaggtgc gccaacgcga tacgcgcccc cttctccgcc gaatagtggg acgggatcag atacggtttc acctccggcg ctatctgaca cgccgccagc gccgccgcgt aggagagaaa gccatccagc accaccggca gcccacagga cgccgccccc agcatgacac cggtcatccc cagcagatcg aaaccaccga ctttgctcag cacatcgagg ccatcattcg ggtcgggctg attgacggcg atagcccgcc gcaccacctc caccttgttg ccaaccttcg ccagcggcag attggcgcca atcccgacca cctcctgcgc atcgctgccg gtcaggacgc tgacgatcgc cgccgccggc gtggtattgg ccatccccag ttcgccgacg ccaaacaggg taacgccctc ctgggccagc gcgcgggtgt agcgcatcac ctccagcaac agctcctgcc cctgctcacg gctcatcgcc ggaccgcggg caatattgcc gcagccccgc gccacccgca tattcaccac gccggggagc ggtacgctat cgataccgac atcaattaca tgcactttcg ccccggcctg ggcggcgagc acgcagacgc cggtggtgcc gcgggtcata tttgccgcct ggatggcggt gaccgccttc ggcgaaatcg ccaccccttc gtcccacact ccatggtcgg cgcacatcac cagcaaggcc tttttcgcca cctgcggctg gccgcccagg ccgggcattc ccgccagctg caccgccagc gcctccagac gcccgaggct gccggggggc ttgagtaagc cgtcgatgtg ttgctgtgcc cgcgccatgg cgtcggcgtc cggcgcgggg atcgcccgca gaaccgtgtt taaattttgc atatggtggt ggtggtggtg agccat

Expected Protein Sequence

MAHHHHHHMQ NLNTVLRAIP APDADAMARA QQHIDGLLKP PGSLGRLEAL AVQLAGMPGL GGQPQVAKKA LLVMCADHGV WDEGVAISPK AVTAIQAANM TRGTTGVCVL AAQAGAKVHV IDVGIDSEPL PGVVNMRVAR GCGNIARGPA MSREQGQELL LEVMRYTRAL AQEGVTLFGV GELGMANTTP AAAIVSVLTG SDAQEVVGIG ANLPLAKVGN KMEVVRRAIA VNQPDPNDGL DVLSKVGGFD LLGMTGVMLG AASCGLPVVL DGFLSYAAAL AACQIAPEVK PYLIPSHYSA EKGARIALAH LGLEPYLNMG MRLGEGSGAA LAMPIVEAAC AMYHRMGMLA ASNIVLPKG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaaa atttaaacac ggttctgcgg gcgatccccg cgccggacgc cgacgccatg gcgcgggcac agcaacacat cgacggctta ctcaagcccc ccggcagcct cgggcgtctg gaggcgctgg cggtgcagct ggcgggaatg cccggcctgg gcggccagcc gcaggtggcg aaaaaggcct tgctggtgat gtgcgccgac catggagtgt gggacgaagg ggtggcgatt tcgccgaagg cggtcaccgc catccaggcg gcaaatatga cccgcggcac caccggcgtc tgcgtgctcg ccgcccaggc cggggcgaaa gtgcatgtaa ttgatgtcgg tatcgatagc gaaccgctgc ccggcgtggt gaatatgcgg gtggcgcggg gctgcggcaa tattgcccgc ggtccggcga tgagccgtga gcaggggcag gagctgttgc tggaggtgat gcgctacacc cgcgcgctgg cccaggaggg cgttaccctg tttggcgtcg gcgaactggg gatggccaat accacgccgg cggcagcgat cgtcagcgtc ctgaccggca gcgatgcgca ggaggtggtc gggattggcg ccaatctgcc gctggcgaag gttggcaaca agatggaggt ggtgcggcgg gctatcgccg tcaatcagcc cgacccgaat gatggcctcg acgtactgag caaagtcggt ggtttcgatc tgctggggat gaccggtgtc atgctggggg cggcgtcctg tgggctgccg gtggtgctgg atggctttct ctcctacgcg gcggcgctgg cggcgtgtca gatagcgccg gaggtgaaac cgtatctgat cccgtcccac tattcggcgg agaagggggc gcgtatcgcg ttggcgcacc tgggcctcga accgtatctc aatatgggca tgcgtctggg agaaggcagc ggcgcggcgc tggcgatgcc gatcgtcgag gcggcctgcg cgatgtacca tcgcatgggc atgctggcgg caagcaatat tgtgttaccg aaggggtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t

Details for KlpnC.20443.a.B1.PW39061

PURIFICATION DATe:	7/25/2021
CONCENTRATION:	30mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAA ATTTAAACAC GGTTCTGCGG GCGATCCCCG CGCCGGACGC CGACGCCATG GCGCGGGCAC AGCAACACAT CGACGGCTTA CTCAAGCCCC CCGGCAGCCT CGGGCGTCTG GAGGCGCTGG CGGTGCAGCT GGCGGGAATG CCCGGCCTGG GCGGCCAGCC GCAGGTGGCG AAAAAGGCCT TGCTGGTGAT GTGCGCCGAC CATGGAGTGT GGGACGAAGG GGTGGCGATT TCGCCGAAGG CGGTCACCGC CATCCAGGCG GCAAATATGA CCCGCGGCAC CACCGGCGTC TGCGTGCTCG CCGCCCAGGC CGGGGCGAAA GTGCATGTAA TTGATGTCGG TATCGATAGC GAACCGCTGC CCGGCGTGGT GAATATGCGG GTGGCGCGGG GCTGCGGCAA TATTGCCCGC GGTCCGGCGA TGAGCCGTGA GCAGGGGCAG GAGCTGTTGC TGGAGGTGAT GCGCTACACC CGCGCGCTGG CCCAGGAGGG CGTTACCCTG TTTGGCGTCG GCGAACTGGG GATGGCCAAT ACCACGCCGG CGGCAGCGAT CGTCAGCGTC CTGACCGGCA GCGATGCGCA GGAGGTGGTC GGGATTGGCG CCAATCTGCC GCTGGCGAAG GTTGGCAACA AGATGGAGGT GGTGCGGCGG GCTATCGCCG TCAATCAGCC CGACCCGAAT GATGGCCTCG ACGTACTGAG CAAAGTCGGT GGTTTCGATC TGCTGGGGAT GACCGGTGTC ATGCTGGGGG CGGCGTCCTG TGGGCTGCCG GTGGTGCTGG ATGGCTTTCT CTCCTACGCG GCGGCGCTGG CGGCGTGTCA GATAGCGCCG GAGGTGAAAC CGTATCTGAT CCCGTCCCAC TATTCGGCGG AGAAGGGGGC GCGTATCGCG TTGGCGCACC TGGGCCTCGA ACCGTATCTC AATATGGGCA TGCGTCTGGG AGAAGGCAGC GGCGCGGCGC TGGCGATGCC GATCGTCGAG GCGGCCTGCG CGATGTACCA TCGCATGGGC ATGCTGGCGG CAAGCAATAT TGTGTTACCG AAGGGGTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T