KlpnC.20440.a

Formate dehydrogenase iron-sulfur subunit

CENTER ID: KlpnC.20440.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20440.a.B1.GE44407 Full length(KlpnC.20440.a) 1 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.38
UniProt: A0A0H3GLC3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAYQSQDIIR RSATNGFTPA PQARDHQQEV AKLIDVTTCI GCKACQVACS EWNDIRDEVG HNVGVYDNPA DLTAKSWTVM RFSEVEQNDK LEWLIRKDGC MHCADPGCLK ACPSEGAIIQ YANGIVDFQS EQCIGCGYCI AGCPFDVPRL NPEDNRVYKC TLCVDRVTVG QEPACVKTCP TGAIHFGSKE DMKTLAGERV AELKTRGYDN AGLYDPAGVG GTHVMYVLHH ADKPNLYHGL PENPEISQTV KFWKGIWKPL AAVGFAATFA ASIFHYVGVG PNRAEEEEDN LHEEKDEVRK
NT Sequence
ATGGCTTACC AATCGCAAGA TATTATTCGT CGTTCCGCGA CTAACGGTTT CACCCCCGCG CCTCAGGCGC GGGACCACCA GCAGGAGGTG GCGAAGCTTA TCGACGTCAC CACCTGTATC GGCTGCAAAG CCTGTCAGGT GGCCTGCTCC GAATGGAACG ATATTCGTGA TGAAGTCGGT CACAATGTCG GGGTGTATGA CAACCCGGCG GACCTGACCG CCAAGTCATG GACGGTCATG CGCTTCTCGG AGGTGGAACA AAACGACAAG CTGGAATGGC TCATCCGCAA GGATGGCTGC ATGCACTGCG CGGACCCGGG CTGCCTGAAG GCCTGCCCGT CAGAAGGCGC GATCATTCAG TACGCCAACG GTATTGTCGA CTTCCAGTCC GAGCAGTGCA TCGGCTGCGG CTACTGTATC GCCGGCTGTC CGTTCGATGT ACCGCGTCTG AACCCGGAAG ACAACCGCGT CTACAAATGT ACCCTGTGCG TAGACCGCGT GACCGTCGGC CAGGAGCCTG CCTGCGTGAA AACCTGTCCG ACCGGCGCAA TCCACTTTGG CTCCAAAGAG GATATGAAAA CGCTGGCCGG CGAACGCGTG GCTGAGCTGA AAACCCGCGG TTATGACAAT GCGGGCCTGT ACGATCCGGC CGGCGTCGGT GGCACCCACG TCATGTACGT GCTGCACCAT GCCGACAAGC CGAATCTGTA CCACGGCCTG CCGGAAAACC CGGAGATCAG CCAGACCGTC AAGTTCTGGA AAGGCATCTG GAAACCGCTG GCGGCCGTCG GCTTCGCCGC GACCTTCGCA GCCAGTATCT TCCACTACGT CGGCGTCGGT CCGAACCGCG CGGAAGAGGA AGAGGACAAT CTGCATGAAG AGAAAGACGA GGTGCGCAAA
Details for KlpnC.20440.a.B1.GE44407
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA YQSQDIIRRS ATNGFTPAPQ ARDHQQEVAK LIDVTTCIGC KACQVACSEW NDIRDEVGHN VGVYDNPADL TAKSWTVMRF SEVEQNDKLE WLIRKDGCMH CADPGCLKAC PSEGAIIQYA NGIVDFQSEQ CIGCGYCIAG CPFDVPRLNP EDNRVYKCTL CVDRVTVGQE PACVKTCPTG AIHFGSKEDM KTLAGERVAE LKTRGYDNAG LYDPAGVGGT HVMYVLHHAD KPNLYHGLPE NPEISQTVKF WKGIWKPLAA VGFAATFAAS IFHYVGVGPN RAEEEEDNLH EEKDEVRK
Validated NT Sequence
tactcactta tttgcgcacc tcgtctttct cttcatgcag attgtcctct tcctcttccg cgcggttcgg accgacgccg acgtagtgga agatactggc tgcgaaggtc gcggcgaagc cgacggccgc cagcggtttc cagatgcctt tccagaactt gacggtctgg ctgatctccg ggttttccgg caggccgtgg tacagattcg gcttgtcggc atggtgcagc acgtacatga cgtgggtgcc accgacgccg gccggatcgt acaggcccgc attgtcataa ccgcgggttt tcagctcagc cacgcgttcg ccggccagcg ttttcatatc ctctttggag ccaaagtgga ttgcgccggt cggacaggtt ttcacgcagg caggctcctg gccgacggtc acgcggtcta cgcacagggt acatttgtag acgcggttgt cttccgggtt cagacgcggt acatcgaacg gacagccggc gatacagtag ccgcagccga tgcactgctc ggactggaag tcgacaatgc cgttggcgta ctgaatgatc gcgccttctg acgggcaggc cttcaggcag cccgggtccg cgcagtgcat gcagccatcc ttgcggatga gccattccag cttgtcgttt tgttccacct ccgagaagcg catgaccgtc catgacttgg cggtcaggtc cgccgggttg tcatacaccc cgacattgtg accgacttca tcacgaatat cgttccattc ggagcaggcc acctgacagg ctttacagcc gatacaggtg gtgacgtcga taagcttcgc cacctcctgc tggtggtccc gcgcctgagg cgcgggggtg aaaccgttag tcgcggaacg acgaataata tcttgcgatt ggtaagccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMA YQSQDIIRRS ATNGFTPAPQ ARDHQQEVAK LIDVTTCIGC KACQVACSEW NDIRDEVGHN VGVYDNPADL TAKSWTVMRF SEVEQNDKLE WLIRKDGCMH CADPGCLKAC PSEGAIIQYA NGIVDFQSEQ CIGCGYCIAG CPFDVPRLNP EDNRVYKCTL CVDRVTVGQE PACVKTCPTG AIHFGSKEDM KTLAGERVAE LKTRGYDNAG LYDPAGVGGT HVMYVLHHAD KPNLYHGLPE NPEISQTVKF WKGIWKPLAA VGFAATFAAS IFHYVGVGPN RAEEEEDNLH EEKDEVRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctt accaatcgca agatattatt cgtcgttccg cgactaacgg tttcaccccc gcgcctcagg cgcgggacca ccagcaggag gtggcgaagc ttatcgacgt caccacctgt atcggctgca aagcctgtca ggtggcctgc tccgaatgga acgatattcg tgatgaagtc ggtcacaatg tcggggtgta tgacaacccg gcggacctga ccgccaagtc atggacggtc atgcgcttct cggaggtgga acaaaacgac aagctggaat ggctcatccg caaggatggc tgcatgcact gcgcggaccc gggctgcctg aaggcctgcc cgtcagaagg cgcgatcatt cagtacgcca acggtattgt cgacttccag tccgagcagt gcatcggctg cggctactgt atcgccggct gtccgttcga tgtaccgcgt ctgaacccgg aagacaaccg cgtctacaaa tgtaccctgt gcgtagaccg cgtgaccgtc ggccaggagc ctgcctgcgt gaaaacctgt ccgaccggcg caatccactt tggctccaaa gaggatatga aaacgctggc cggcgaacgc gtggctgagc tgaaaacccg cggttatgac aatgcgggcc tgtacgatcc ggccggcgtc ggtggcaccc acgtcatgta cgtgctgcac catgccgaca agccgaatct gtaccacggc ctgccggaaa acccggagat cagccagacc gtcaagttct ggaaaggcat ctggaaaccg ctggcggccg tcggcttcgc cgcgaccttc gcagccagta tcttccacta cgtcggcgtc ggtccgaacc gcgcggaaga ggaagaggac aatctgcatg aagagaaaga cgaggtgcgc aaatgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat