KlpnC.20439.b

Putative alcohol dehydrogenase

CENTER ID: KlpnC.20439.b
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20439.b.B1.GE44406 Full length(KlpnC.20439.b) 1 387
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4450
UniProt: A0A0H3GYI2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNNFDLHTPT RILFGKGAIE KLREQIPAEA RVLITYGGGS VKKTGVLDQV LTALNGLDVL EFGGIEPNPS YETLMNAVKL AREEKVTFLL AVGGGSVLDG TKFIAAAAHY DADIDPWEIL ETYGSKIASA IPMGSVLTLP ATGSESNKGA VISRKTTGDK RAFMSSHVQP QFAILDPVYT YTLPPRQVAN GVVDAFVHTL EQYVTYPVDG KIQDRFAEGI LLTLIEDGPK ALQEPENYNV RANIMWAATQ ALNGLIGAGV PQDWATHMLG HELTAMHGLD HAQTLAIVLP ALWNEKRDAK REKLLQYAER VWNITEGSDD QRIDAAIAAT RQFFEQMGVP TRLSDYGLDG SSIPALLAKL EEHGMTKLGE HQDITLDVSR RIYEAAR
NT Sequence
ATGAATAATT TCGACCTGCA TACCCCAACC CGCATTCTGT TTGGCAAAGG CGCGATTGAA AAGCTGCGTG AACAGATCCC GGCGGAAGCC CGCGTACTGA TCACCTACGG CGGCGGCAGC GTCAAAAAAA CAGGCGTCCT GGATCAGGTC CTCACCGCTC TGAATGGCCT GGATGTCCTT GAATTTGGCG GCATCGAGCC GAACCCGTCT TACGAAACCC TGATGAATGC GGTGAAACTC GCCCGGGAAG AGAAAGTGAC CTTCCTGCTG GCGGTCGGCG GCGGTTCGGT GCTGGATGGC ACCAAGTTTA TCGCTGCAGC AGCCCACTAC GACGCGGATA TCGATCCGTG GGAAATTCTG GAAACCTATG GCAGCAAAAT TGCCAGCGCC ATTCCAATGG GCTCGGTACT GACTCTGCCG GCGACCGGTT CTGAATCCAA CAAAGGCGCG GTCATATCGC GGAAAACCAC CGGCGACAAA CGCGCGTTTA TGTCTTCGCA CGTCCAGCCG CAGTTCGCGA TCCTCGATCC GGTTTATACC TATACCCTGC CGCCGCGCCA GGTCGCGAAC GGCGTGGTTG ACGCCTTCGT CCATACCCTC GAGCAGTACG TGACCTACCC GGTTGACGGC AAAATCCAGG ACCGCTTCGC CGAAGGCATT CTCCTGACCC TGATCGAGGA TGGCCCGAAA GCCCTGCAGG AACCGGAGAA CTATAACGTG CGCGCCAATA TTATGTGGGC GGCGACGCAG GCGCTGAACG GCCTGATCGG CGCAGGCGTG CCACAGGACT GGGCGACGCA TATGCTCGGC CACGAGCTGA CGGCGATGCA CGGCCTGGAT CACGCCCAGA CGCTGGCTAT CGTGCTGCCG GCGCTGTGGA ATGAGAAACG CGATGCCAAG CGCGAGAAGC TGCTGCAGTA TGCCGAGCGC GTGTGGAATA TTACCGAAGG CTCTGACGAC CAACGTATCG ATGCCGCCAT CGCCGCAACG CGTCAGTTCT TCGAGCAGAT GGGCGTGCCG ACCCGCCTTT CCGATTACGG TCTCGACGGT AGCTCCATCC CGGCGCTGCT GGCGAAACTG GAAGAGCACG GCATGACTAA ACTGGGCGAA CATCAGGATA TCACCCTGGA CGTCAGCCGC CGTATCTACG AGGCTGCACG C
Details for KlpnC.20439.b.B1.GE44406
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN NFDLHTPXRI LFGKGAIEKL REQIPAEARV LITYGGGSVK KTGVLDQVLT ALNGLDVLEF GGIEPNPSYE TLMNAVKLAR EEKVTFLLAV GGGSVLDGTK FIAAAAHYDA DIDPWEILET YGSKIASAIP MGSVLTLPAT GSESNKGAVI SRKTTGDKRA FMSSHVQPQF AILDPVYTYT LPPRQVANGV VDAFVHTVEQ YVTYPVDGKI QDRFAEGILL TLIEDGPKAL QEPENYNVRA NIMWAATQAL NGLIGAGVPQ DWATHMLGHE LTAMHGLDHA QTLAIVLPAL WNEKRDAKRE KLLQYAERVW NITEGSDDQR IDAAIAATRQ FFEQMGVPTR LSDYGLDGSS IPALLAKLEE HGMTKLGEHQ DITLDVS
Validated NT Sequence
cggctgacgt ccagggtgat atcctgatgt tcgccaagtt tagtcatgcc gtgctcttcc agtttcgcca gcagcgccgg gatggagcta ccgtcgagac cgtaatcgga aaggcgggtc ggcacgccca tctgctcgaa gaactgacgc gttgcggcga tggcggcatc gatacgttgg tcgtcagagc cttcggtaat attccacacg cgctcggcat actgcagcag cttctcgcgc ttggcatcgc gtttctcatt ccacagcgcc ggcagcacga tagccagcgt ctgggcgtga tccaggccgt gcatcgccgt cagctcgtgg ccgagcatat gcgtcgccca gtcctgtggc acgcctgcgc cgatcaggcc gttcagcgcc tgcgtcgccg cccacataat attggcgcgc acgttatagt tctccggttc ctgcagggct ttcgggccat cctcgatcag ggtcaggaga atgccttcgg cgaaacggtc ctggattttg ccgtctaccg ggtaggtcac gtactgctcg acggtatgga cgaaggcgtc aaccacgccg ttcgcgacct ggcgcggcgg cagggtatag gtataaaccg gatcgaggat cgcgaactgc ggctggacgt gcgaagacat aaacgcgcgt ttgtcgccgg tggttttccg cgatatgacc gcgcctttgt tggattcaga accggtcgcc ggcagagtca gtaccgagcc cattggaatg gcgctggcaa ttttgctgcc ataggtttcc agaatttccc acggatcgat atccgcgtcg tagtgggctg ctgcagcgat aaacttggtg ccatccagca ccgaaccgcc gccgaccgcc agcaggaagg tcactttctc ttcccgggcg agtttcaccg cattcatcag ggtttcgtaa gacgggttcg gctcgatgcc gccaaattca aggacatcca ggccattcag agcggtgagg acctgatcca ggacgcctgt ttttttgacg ctgccgccgc cgtaggtgat cagtacgcgg gcttccgccg ggatctgttc acgcagcttt tcaatcgcgc ctttgccaaa cagaatgcgg nnnggggtat gcaggtcgaa attattcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMN NFDLHTPTRI LFGKGAIEKL REQIPAEARV LITYGGGSVK KTGVLDQVLT ALNGLDVLEF GGIEPNPSYE TLMNAVKLAR EEKVTFLLAV GGGSVLDGTK FIAAAAHYDA DIDPWEILET YGSKIASAIP MGSVLTLPAT GSESNKGAVI SRKTTGDKRA FMSSHVQPQF AILDPVYTYT LPPRQVANGV VDAFVHTLEQ YVTYPVDGKI QDRFAEGILL TLIEDGPKAL QEPENYNVRA NIMWAATQAL NGLIGAGVPQ DWATHMLGHE LTAMHGLDHA QTLAIVLPAL WNEKRDAKRE KLLQYAERVW NITEGSDDQR IDAAIAATRQ FFEQMGVPTR LSDYGLDGSS IPALLAKLEE HGMTKLGEHQ DITLDVSRRI YEAAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaata atttcgacct gcatacccca acccgcattc tgtttggcaa aggcgcgatt gaaaagctgc gtgaacagat cccggcggaa gcccgcgtac tgatcaccta cggcggcggc agcgtcaaaa aaacaggcgt cctggatcag gtcctcaccg ctctgaatgg cctggatgtc cttgaatttg gcggcatcga gccgaacccg tcttacgaaa ccctgatgaa tgcggtgaaa ctcgcccggg aagagaaagt gaccttcctg ctggcggtcg gcggcggttc ggtgctggat ggcaccaagt ttatcgctgc agcagcccac tacgacgcgg atatcgatcc gtgggaaatt ctggaaacct atggcagcaa aattgccagc gccattccaa tgggctcggt actgactctg ccggcgaccg gttctgaatc caacaaaggc gcggtcatat cgcggaaaac caccggcgac aaacgcgcgt ttatgtcttc gcacgtccag ccgcagttcg cgatcctcga tccggtttat acctataccc tgccgccgcg ccaggtcgcg aacggcgtgg ttgacgcctt cgtccatacc ctcgagcagt acgtgaccta cccggttgac ggcaaaatcc aggaccgctt cgccgaaggc attctcctga ccctgatcga ggatggcccg aaagccctgc aggaaccgga gaactataac gtgcgcgcca atattatgtg ggcggcgacg caggcgctga acggcctgat cggcgcaggc gtgccacagg actgggcgac gcatatgctc ggccacgagc tgacggcgat gcacggcctg gatcacgccc agacgctggc tatcgtgctg ccggcgctgt ggaatgagaa acgcgatgcc aagcgcgaga agctgctgca gtatgccgag cgcgtgtgga atattaccga aggctctgac gaccaacgta tcgatgccgc catcgccgca acgcgtcagt tcttcgagca gatgggcgtg ccgacccgcc tttccgatta cggtctcgac ggtagctcca tcccggcgct gctggcgaaa ctggaagagc acggcatgac taaactgggc gaacatcagg atatcaccct ggacgtcagc cgccgtatct acgaggctgc acgctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat