KlpnC.20439.a

L-1,2-propanediol oxidoreductase

CENTER ID: KlpnC.20439.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
KlpnC.20439.a.B1.GE44405 Full length(KlpnC.20439.a) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|1125630.4.peg.4130
UniProt: A0A0H3GUJ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MANRMILNET AWFGRGAINA LTDEAVRRGY RKALIVTDST LARCGVAAKV TDKLDAAGLA WDIFSDVIPN PTIAVVQQGR QAFQRSGADY LIAIGGGSPQ DTCKAIGIIQ RNPEFADVRS LEGLSPTRQP SVPIFAVPTT AGTAAEVTIN YVITDEEQRR KFVCVDPHDI PQVAFIDADM MDAMPPALKA ATGVDALTHA IEGYLTRGAW ALTDALHLKA IEIIAGALRG SVAGDAGAGE AMALGQYVAG MGFSNVGLGL VHGMAHPLGA FYNTPHGVAN AILLPHVMRF NAEATGEKYR DIARAMGVRV EALSLTAARE AAVEAVCQLN RDVGIPGHLR EVGVRKEDIP ALAQAALADV CTGGNPREAS LADIVGLYQA AW
NT Sequence
ATGGCAAACA GAATGATCCT CAATGAAACG GCATGGTTTG GCCGGGGGGC GATAAACGCA TTAACCGATG AAGCGGTGCG GCGCGGTTAT CGTAAAGCAT TGATCGTCAC TGACAGCACT CTCGCCCGCT GCGGCGTGGC GGCGAAAGTC ACCGACAAAC TGGATGCCGC CGGGCTGGCA TGGGATATAT TCAGCGACGT GATCCCTAAC CCGACCATCG CCGTGGTCCA GCAGGGGCGG CAGGCCTTTC AACGCAGCGG GGCGGATTAC CTGATCGCCA TCGGCGGCGG TTCGCCGCAG GATACCTGTA AGGCGATCGG TATTATTCAG CGTAACCCGG AATTCGCCGA TGTCCGTAGC CTCGAGGGGC TGTCGCCCAC CCGTCAGCCC AGCGTGCCGA TCTTCGCGGT GCCGACCACC GCGGGTACCG CGGCGGAGGT GACGATTAAC TATGTCATCA CTGATGAAGA ACAGCGGCGC AAATTCGTCT GTGTCGACCC GCACGATATT CCGCAGGTGG CCTTTATCGA TGCCGATATG ATGGACGCTA TGCCGCCGGC GCTGAAGGCG GCTACCGGCG TGGACGCCCT GACGCATGCT ATCGAAGGGT ACCTCACTCG CGGCGCCTGG GCTTTGACCG ATGCTCTGCA CCTCAAGGCC ATCGAGATCA TCGCCGGCGC GCTGCGCGGC TCGGTTGCCG GCGACGCCGG CGCGGGGGAA GCTATGGCGC TCGGCCAGTA TGTGGCGGGG ATGGGATTTT CCAACGTCGG GCTCGGGCTG GTGCACGGCA TGGCGCATCC GCTCGGCGCT TTTTACAACA CGCCGCACGG CGTGGCGAAT GCCATCCTGC TGCCGCACGT GATGCGCTTT AATGCTGAGG CGACCGGGGA AAAATATCGC GACATCGCCC GGGCGATGGG CGTCAGGGTG GAAGCGTTAA GCCTGACCGC CGCGCGCGAG GCGGCAGTAG AGGCGGTCTG CCAGCTTAAC CGCGATGTCG GTATTCCCGG CCATCTGCGC GAGGTGGGCG TCAGGAAAGA GGATATTCCG GCGCTGGCCC AGGCGGCGCT GGCGGATGTG TGTACCGGCG GCAACCCGCG CGAGGCGAGC CTGGCGGATA TCGTCGGGTT ATATCAGGCT GCCTGG
Details for KlpnC.20439.a.B1.GE44405
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 41kDa
OBSERVED MW: 41kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMA NRMILNETAW FGRGAINALT DEAVRRGYRK ALIVTDSTLA RCGVAAKVTD KLDAAGLAWD MFSDVIPNPT IAVVQQGLQA FQRSGADYLI AIGGGSPQDT CKAIGIIQRN PEFADVRSLE GLSPTRQPSV PIFAVPTTAG TAAEVTINYV ITDEEQRRKF VCVDPHDIPQ VAFIDADMMD AMPPALKAAT GVDALTHAIE GYLTRGAWAL TDALHLKAIE IIAGALRGSV AGDAGAGEAM ALGQYVAGMG FSNVGLGLVH GMAHPLGAFY NTPHGVANAI LLPHVMRFNA EATGEKYRDI ARAMGVRVEA LSLTAAREAA VEAVCQLNRD VVIPGHLREV GVRKEDIPAL AQAALADVCT GGNPREASLA DI
Validated NT Sequence
acgatatccg ccaggctcgc ctcgcgcggg ttgccgccgg tacacacatc cgccagcgcc gcctgggcca gcgccggaat atcctctttc ctgacgccca cctcgcgcag atggccggga ataacgacat cgcggttaag ctggcagacc gcctctactg ccgcctcgcg cgcggcggtc aggcttaacg cttccaccct gacgcccatc gcccgggcga tgtcgcgata tttttccccg gtcgcctcag cattaaagcg catcacgtgc ggcagcagga tggcattcgc cacgccgtgc ggcgtgttgt aaaaagcgcc gagcggatgc gccatgccgt gcaccagccc gagcccgacg ttggaaaatc ccatccccgc cacatactgg ccgagcgcca tagcttcccc cgcgcctgcg tcgccggcaa ccgagccgcg cagcgcgccg gcgatgatct cgatggcctt gaggtgcaga gcatcggtca aagcccaggc gccgcgagtg aggtaccctt cgatagcatg cgtcagggcg tccacgccgg tagccgcctt cagcgccggc ggcatagcgt ccatcatatc ggcatcgata aaggccacct gcggaatatc gtgcgggtcg acacagacga atttgcgccg ctgttcttca tcagtgatga catagttaat cgtcacctcc gccgcggtac ccgcggtggt cggcaccgcg aagatcggca cgctgggctg acgggtgggc gacagcccct cgaggctacg gacatcagcg aattccgggt tacgctgaat aataccgatc gccttacagg tatcctgcgg cgaaccgccg ccgatggcga tcaggtaatc cgccccgctg cgttgaaagg cctgcagccc ctgctggacc acggcgatgg tcgggttagg gatcacgtcg ctgaacatat cccatgccag cccggcggca tccagtttgt cggtgacttt cgccgccacg ccgcagcggg cgagagtgct gtcggtgacg atcaatgctt tacgatagcc gcgccgcacc gcttcatcgg ttaatgcgtt tatcgccccc cggccaaacc atgccgtttc attgaggatc attctgtttg ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMA NRMILNETAW FGRGAINALT DEAVRRGYRK ALIVTDSTLA RCGVAAKVTD KLDAAGLAWD IFSDVIPNPT IAVVQQGRQA FQRSGADYLI AIGGGSPQDT CKAIGIIQRN PEFADVRSLE GLSPTRQPSV PIFAVPTTAG TAAEVTINYV ITDEEQRRKF VCVDPHDIPQ VAFIDADMMD AMPPALKAAT GVDALTHAIE GYLTRGAWAL TDALHLKAIE IIAGALRGSV AGDAGAGEAM ALGQYVAGMG FSNVGLGLVH GMAHPLGAFY NTPHGVANAI LLPHVMRFNA EATGEKYRDI ARAMGVRVEA LSLTAAREAA VEAVCQLNRD VGIPGHLREV GVRKEDIPAL AQAALADVCT GGNPREASLA DIVGLYQAAW
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa acagaatgat cctcaatgaa acggcatggt ttggccgggg ggcgataaac gcattaaccg atgaagcggt gcggcgcggt tatcgtaaag cattgatcgt cactgacagc actctcgccc gctgcggcgt ggcggcgaaa gtcaccgaca aactggatgc cgccgggctg gcatgggata tattcagcga cgtgatccct aacccgacca tcgccgtggt ccagcagggg cggcaggcct ttcaacgcag cggggcggat tacctgatcg ccatcggcgg cggttcgccg caggatacct gtaaggcgat cggtattatt cagcgtaacc cggaattcgc cgatgtccgt agcctcgagg ggctgtcgcc cacccgtcag cccagcgtgc cgatcttcgc ggtgccgacc accgcgggta ccgcggcgga ggtgacgatt aactatgtca tcactgatga agaacagcgg cgcaaattcg tctgtgtcga cccgcacgat attccgcagg tggcctttat cgatgccgat atgatggacg ctatgccgcc ggcgctgaag gcggctaccg gcgtggacgc cctgacgcat gctatcgaag ggtacctcac tcgcggcgcc tgggctttga ccgatgctct gcacctcaag gccatcgaga tcatcgccgg cgcgctgcgc ggctcggttg ccggcgacgc cggcgcgggg gaagctatgg cgctcggcca gtatgtggcg gggatgggat tttccaacgt cgggctcggg ctggtgcacg gcatggcgca tccgctcggc gctttttaca acacgccgca cggcgtggcg aatgccatcc tgctgccgca cgtgatgcgc tttaatgctg aggcgaccgg ggaaaaatat cgcgacatcg cccgggcgat gggcgtcagg gtggaagcgt taagcctgac cgccgcgcgc gaggcggcag tagaggcggt ctgccagctt aaccgcgatg tcggtattcc cggccatctg cgcgaggtgg gcgtcaggaa agaggatatt ccggcgctgg cccaggcggc gctggcggat gtgtgtaccg gcggcaaccc gcgcgaggcg agcctggcgg atatcgtcgg gttatatcag gctgcctggt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat