KlpnC.20159.a

Ribonucleoside-diphosphate reductase

CENTER ID: KlpnC.20159.a
ORGANISM: Klebsiella pneumoniae subsp. pneumoniae (strain HS11286)
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
unclassified

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
KlpnC.20159.a.B1.GE44401 Full length(KlpnC.20159.a) 1 713
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
PATRIC ID: fig|1125630.4.peg.3982
UniProt: A0A0H3GWI4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATTTAERIT AAVDFHALNA MLNLYDSEGR IPFEKDRQAV EAFMATQVQP NALTFPSQED KLSWLVSEGY YDPQVLAGYD RGFVLALFAH ARRAPFRFQT FLGAWKFYTS YALKTFDGKH YLEDFAERSV MVALTLARGD EQQARQLTEE ILSGRFQPAT PTFLNAGKQQ RGELISCFLL RIEDNMESIG RAVNSALQLS KRGGGVAFLL SNLREAGAPI KRIENQSSGV VPVMKMLEDA FSYANQLGAR QGAGAVWLHV HHPDILRFLD TRRENADEKI RIKTLSLGVV IPDITFQLAK EDAQMALFSP YDVERLYGKP FADCAIGDLY PQLVADERVR KRWIRARDLF QRLAEIQFES GYPYIMFEDT VNRASPVAGR VTMSNLCSEI LQVSTPSAYN EDLSYAHIGE DISCNLGSLN IAHTMDSPDF GRTIATAVRA LTAVSDMSDI QSVPSVAAGN AASHAIGLGQ MNLHGYLARE GIAYGSPEGL DFTNIYFYTV TWHALHTSMQ LARERGQRFA GFEQSRYASG AYFDKYLQEE WQPKTERVRT LFARAGISLP DRESWRQLRD DVMRYGIYNR YLQAVPPTGS ISYINHATSS IHPIVSKIEI RKEGKTGRVY YPAPFMNNDN LALYQDAYEI GPEKIIDTYA EATRHVDQGL SLTLFFPDTA TTRDINKAQI YAWKKGIKTL YYIRLRQLAL EGTEIEGCVS CAL
NT Sequence
TTGGCAACGA CAACCGCAGA ACGTATAACC GCCGCCGTGG ATTTTCACGC CCTCAACGCG ATGCTGAATT TGTACGACAG CGAGGGCCGC ATCCCGTTTG AAAAAGATCG CCAGGCGGTT GAGGCCTTTA TGGCGACCCA GGTTCAGCCC AACGCCCTCA CCTTCCCCAG CCAGGAGGAT AAGCTCAGCT GGCTCGTCAG CGAGGGCTAT TACGATCCGC AGGTGCTGGC CGGCTATGAC CGCGGGTTTG TGCTGGCGTT GTTCGCCCAT GCCCGGCGCG CTCCGTTTCG CTTTCAGACC TTCCTCGGCG CCTGGAAGTT CTATACCAGC TATGCGCTGA AGACCTTTGA CGGCAAGCAC TACCTCGAGG ATTTCGCCGA ACGCAGCGTG ATGGTGGCCC TGACGCTGGC CCGGGGCGAT GAACAGCAGG CGCGGCAGCT GACGGAGGAG ATACTCAGCG GCCGCTTTCA GCCCGCCACA CCGACCTTCC TTAACGCTGG CAAACAGCAG CGCGGGGAGC TGATCTCCTG CTTCCTGCTG CGTATTGAGG ATAATATGGA GTCCATCGGC CGGGCGGTGA ACTCGGCGCT ACAGCTGTCG AAGCGCGGCG GCGGGGTGGC CTTTTTACTC TCCAACCTGC GCGAGGCCGG GGCGCCGATC AAGCGCATCG AAAACCAGTC TTCTGGGGTG GTGCCGGTGA TGAAGATGCT GGAGGACGCC TTCTCCTATG CCAATCAGCT CGGCGCCCGC CAGGGGGCGG GTGCGGTCTG GCTGCATGTT CACCATCCCG ATATTTTACG CTTTCTTGAT ACCCGGCGTG AAAATGCCGA TGAGAAAATC CGCATCAAAA CCTTGTCGCT GGGGGTGGTG ATCCCTGATA TCACCTTCCA GCTGGCGAAA GAAGATGCGC AGATGGCCCT GTTCTCGCCT TATGACGTCG AACGGCTGTA CGGCAAACCT TTCGCCGATT GCGCCATCGG CGACCTCTAT CCGCAGCTGG TGGCCGACGA GCGGGTCCGC AAACGCTGGA TCCGCGCCCG CGACCTGTTC CAGCGCCTGG CGGAGATCCA GTTTGAATCC GGCTACCCGT ACATCATGTT TGAAGACACC GTGAACCGCG CCAGTCCGGT GGCCGGGCGG GTCACCATGA GCAATCTGTG TTCGGAGATC CTGCAGGTCA GTACGCCGTC CGCCTATAAT GAAGACCTCA GCTATGCGCA TATCGGGGAA GATATCTCCT GCAACCTCGG CTCGCTGAAC ATCGCCCATA CCATGGACTC GCCGGATTTC GGCCGCACAA TCGCCACCGC GGTGCGCGCC CTGACCGCCG TGTCCGACAT GAGCGACATT CAGTCGGTTC CCTCGGTGGC GGCCGGCAAC GCGGCCTCCC ACGCCATCGG CCTGGGGCAG ATGAATCTGC ACGGTTACCT GGCGCGGGAA GGCATTGCCT ACGGCAGCCC GGAGGGGCTG GACTTCACCA ATATCTACTT CTACACCGTG ACCTGGCACG CGCTGCACAC CTCGATGCAG CTGGCCCGCG AGCGCGGCCA GCGCTTCGCC GGCTTCGAGC AGTCGCGCTA CGCCAGCGGG GCGTACTTCG ACAAATATCT GCAGGAGGAG TGGCAGCCGA AAACCGAACG GGTGCGCACG CTGTTCGCCC GCGCCGGCAT CTCTCTGCCC GACCGGGAAA GCTGGCGCCA GCTGCGCGAC GATGTCATGC GCTATGGCAT CTACAATCGC TATCTGCAGG CGGTGCCGCC CACCGGCTCG ATCTCGTACA TCAACCATGC CACCTCCAGC ATTCACCCGA TCGTCTCGAA GATTGAGATC CGCAAAGAGG GGAAAACCGG GCGGGTGTAC TACCCCGCGC CATTTATGAA TAACGACAAT CTGGCCCTCT ACCAGGATGC GTATGAGATA GGGCCGGAGA AGATCATTGA CACTTACGCC GAGGCGACGC GCCACGTCGA CCAGGGGCTG TCGCTGACCC TGTTCTTCCC CGATACCGCC ACCACCCGCG ATATCAACAA GGCGCAAATT TACGCCTGGA AGAAAGGCAT CAAGACGCTC TACTACATCC GTCTGCGCCA GCTGGCGCTG GAAGGCACTG AAATTGAAGG CTGCGTGTCC TGCGCGCTG
Details for KlpnC.20159.a.B1.GE44401
HARVESTED ON: 6/30/2021
SEQUENCED ON: 7/22/2021
EXPECTED MW: 81kDa
OBSERVED MW: 81kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMA TTTAERITAA VDFHALNAML NLYDSEGRIP FEKDRQAVEA FMATQVQPNA LTFPSQEDKL SWLVSEGYYD PQVLAGYDRG FVLALFAHAR RAPFRFQTFL GAWKFYTSYA LKTFDGKHYL EDFAERSVMV ALTLARGDEQ QARQLTEEIL SGRFQPATPT FLNAGKQQRG ELISCFLLRI EDNMESIGRA VNSALQLSKR GGGVAFLLSN LREAGAPIKR IENQSSGVVP VMKMLEDAFS YANQLGARQG AGAVWLHVHH PDILRFLDTR RENADEKIRI KTLSLGVVIP DITFQLAKED AQMALFSPYD VERLYGKPFA DCAIGDLYPQ LVADERVRKR WIRARDLFQR LAEIQFESGY PYIMFEDTVN RASPVAGRVT MSNLCSEILQ VSTPSAYNED LSYAHIGEDI SCNLGSLNIA HTMDSPDFGR TIATAVRALT AVSDMSDIQS VPSVAAGNAA SHAIGLGQMN LHGYLAREGI AYGSPEGLDF TNIYFYTVTW HALHTSMQLA RERGQRFAGF EQSRYASGAY FDKYLQEEWQ PKTERVRTLF ARAGISLPDR ESWRQLRDDV MRYGIYNRYL QAVPPTGSIS YINHATSSIH PIVSKIEIRK EGKTGRVYYP APFMNNDNLA LYQDAYEIGP EKIIDTYAEA TRHVDQGLSL TLFFPDTATT RDINKAQIYA WKKGIKTLYY IRLRQLALEG TEIEGCVSCA L
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggcaa cgacaaccgc agaacgtata accgccgccg tggattttca cgccctcaac gcgatgctga atttgtacga cagcgagggc cgcatcccgt ttgaaaaaga tcgccaggcg gttgaggcct ttatggcgac ccaggttcag cccaacgccc tcaccttccc cagccaggag gataagctca gctggctcgt cagcgagggc tattacgatc cgcaggtgct ggccggctat gaccgcgggt ttgtgctggc gttgttcgcc catgcccggc gcgctccgtt tcgctttcag accttcctcg gcgcctggaa gttctatacc agctatgcgc tgaagacctt tgacggcaag cactacctcg aggatttcgc cgaacgcagc gtgatggtgg ccctgacgct ggcccggggc gatgaacagc aggcgcggca gctgacggag gagatactca gcggccgctt tcagcccgcc acaccgacct tccttaacgc tggcaaacag cagcgcgggg agctgatctc ctgcttcctg ctgcgtattg aggataatat ggagtccatc ggccgggcgg tgaactcggc gctacagctg tcgaagcgcg gcggcggggt ggccttttta ctctccaacc tgcgcgaggc cggggcgccg atcaagcgca tcgaaaacca gtcttctggg gtggtgccgg tgatgaagat gctggaggac gccttctcct atgccaatca gctcggcgcc cgccaggggg cgggtgcggt ctggctgcat gttcaccatc ccgatatttt acgctttctt gatacccggc gtgaaaatgc cgatgagaaa atccgcatca aaaccttgtc gctgggggtg gtgatccctg atatcacctt ccagctggcg aaagaagatg cgcagatggc cctgttctcg ccttatgacg tcgaacggct gtacggcaaa cctttcgccg attgcgccat cggcgacctc tatccgcagc tggtggccga cgagcgggtc cgcaaacgct ggatccgcgc ccgcgacctg ttccagcgcc tggcggagat ccagtttgaa tccggctacc cgtacatcat gtttgaagac accgtgaacc gcgccagtcc ggtggccggg cgggtcacca tgagcaatct gtgttcggag atcctgcagg tcagtacgcc gtccgcctat aatgaagacc tcagctatgc gcatatcggg gaagatatct cctgcaacct cggctcgctg aacatcgccc ataccatgga ctcgccggat ttcggccgca caatcgccac cgcggtgcgc gccctgaccg ccgtgtccga catgagcgac attcagtcgg ttccctcggt ggcggccggc aacgcggcct cccacgccat cggcctgggg cagatgaatc tgcacggtta cctggcgcgg gaaggcattg cctacggcag cccggagggg ctggacttca ccaatatcta cttctacacc gtgacctggc acgcgctgca cacctcgatg cagctggccc gcgagcgcgg ccagcgcttc gccggcttcg agcagtcgcg ctacgccagc ggggcgtact tcgacaaata tctgcaggag gagtggcagc cgaaaaccga acgggtgcgc acgctgttcg cccgcgccgg catctctctg cccgaccggg aaagctggcg ccagctgcgc gacgatgtca tgcgctatgg catctacaat cgctatctgc aggcggtgcc gcccaccggc tcgatctcgt acatcaacca tgccacctcc agcattcacc cgatcgtctc gaagattgag atccgcaaag aggggaaaac cgggcgggtg tactaccccg cgccatttat gaataacgac aatctggccc tctaccagga tgcgtatgag atagggccgg agaagatcat tgacacttac gccgaggcga cgcgccacgt cgaccagggg ctgtcgctga ccctgttctt ccccgatacc gccaccaccc gcgatatcaa caaggcgcaa atttacgcct ggaagaaagg catcaagacg ctctactaca tccgtctgcg ccagctggcg ctggaaggca ctgaaattga aggctgcgtg tcctgcgcgc tgtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat